FastQCFastQC Report
Wed 25 May 2016
SRR1295310_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295310_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5666787
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT165570.29217614849472906No Hit
GGTATCAACGCAGAGTACTTTTTTT139330.24587124943993835No Hit
TATCAACGCAGAGTACTTTTTTTTT100040.1765374276463894No Hit
GTGGTATCAACGCAGAGTACATGGG81190.1432734281348496No Hit
ACGCAGAGTACTTTTTTTTTTTTTT63910.11277995802559723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3900.012.18831353
CAGCGTC4400.010.8032792
GCAGCGT4700.09.7214391
GGTATCA63900.09.5189091
CGGAGAT1804.0508894E-99.5189091
GGACCGT1002.7675103E-49.4966516
GTCCTAG5950.09.2789361
TATACCG1054.4636725E-49.0541775
TGCACCG950.00181301729.0065235
TCTGTCG3700.08.9830028
GGACCGA850.0074613718.93802456
GTCTTAG5650.08.7607661
CTAGGAC5550.08.7360573
TAGACAG8950.08.7102195
GGACTGT6350.08.6741076
TATGCCG1000.00288785488.5561965
TAGACTG8200.08.4634485
TATACTG13450.08.4112965
GCGTCAG5750.08.2668574
GTCTACG1502.5250334E-58.2497221
Produced by FastQC (version 0.11.5)