FastQCFastQC Report
Wed 25 May 2016
SRR1295309_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295309_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3569342
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG44760.12540126443473334No Hit
GTATCAACGCAGAGTACTTTTTTTT40200.11262580049768278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGC1553.092282E-1011.0416981
GTAGGAC2100.010.406223
GTATTAG6900.09.6459271
GTACTAG3000.09.5081291
TACACCG900.0011126889.5014655
TGCACCG1201.6986976E-59.5014655
GTCCTAA3000.09.1911911
TACACGG1151.10949724E-49.0883585
TCTAGAC3350.08.7922773
CTAGACT2659.094947E-128.60514
GTCTTAC3000.08.5573161
CTAGACA4900.08.5319274
GCGTCAG1803.985624E-78.451671
CTAGGAC2701.4551915E-118.4456283
CCAGGAC5800.08.3546193
GTGGTAT18200.08.3065521
GTCCTAC2803.274181E-118.1498241
TAGACTG3850.08.1441135
GGTATCA30350.08.0513651
CTTAGAC3201.8189894E-128.0167483
Produced by FastQC (version 0.11.5)