##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295309_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3569342
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.094882193973007	31.0	30.0	33.0	26.0	34.0
2	30.110387572835553	31.0	30.0	34.0	25.0	34.0
3	30.374879459575464	31.0	30.0	34.0	26.0	34.0
4	33.53019856320857	35.0	33.0	37.0	27.0	37.0
5	33.94899031810345	35.0	35.0	37.0	28.0	37.0
6	34.12945551308897	36.0	35.0	37.0	29.0	37.0
7	34.04331078389238	37.0	35.0	37.0	29.0	37.0
8	33.98783081027259	36.0	35.0	37.0	28.0	37.0
9	34.97071505056114	38.0	35.0	39.0	27.0	39.0
10	34.88970235970663	38.0	34.0	39.0	27.0	39.0
11	35.327860709340825	39.0	35.0	39.0	28.0	39.0
12	35.319795637403196	39.0	35.0	39.0	27.0	39.0
13	35.23775950861531	38.0	35.0	39.0	27.0	39.0
14	35.42175560649554	39.0	34.0	41.0	25.0	41.0
15	36.04866863416282	39.0	35.0	41.0	27.0	41.0
16	36.13494980307295	39.0	35.0	41.0	27.0	41.0
17	35.9383491411022	39.0	35.0	41.0	27.0	41.0
18	35.910491345463676	39.0	35.0	41.0	27.0	41.0
19	35.9844335454546	39.0	34.0	41.0	27.0	41.0
20	35.97136278899584	39.0	34.0	41.0	27.0	41.0
21	35.965102251339324	39.0	34.0	41.0	27.0	41.0
22	35.68948758622738	39.0	34.0	41.0	26.0	41.0
23	35.35872858358768	39.0	34.0	40.0	25.0	41.0
24	35.69769302017011	39.0	34.0	41.0	26.0	41.0
25	34.32502265123376	38.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4196495441061501
1101	2	0.6484667518659784
1101	3	0.4310608744064197
1101	4	0.3570940984048434
1101	5	0.32314641388419574
1101	6	0.2629186667592265
1101	7	0.30982440503396447
1101	8	0.31824701370696573
1101	9	0.2767346722803268
1101	10	0.5845439496447753
1101	11	0.590277348840516
1101	12	0.6303430816011755
1101	13	0.6148791369048041
1101	14	1.1380032243788136
1101	15	0.8784332468844696
1101	16	0.6359310421526416
1101	17	0.9759778395126659
1101	18	0.9259356078213514
1101	19	0.8423909135958922
1101	20	1.0114081452900052
1101	21	1.0545276951193898
1101	22	0.7854385652846716
1101	23	-1.413841556939957
1101	24	0.1547047572582514
1101	25	0.7564740959061282
1102	1	0.2561707603565857
1102	2	0.3418667838468572
1102	3	0.23967387532598394
1102	4	0.27517491487582646
1102	5	0.18785677262535927
1102	6	0.005511423412137617
1102	7	0.08735100411836072
1102	8	0.09384810681718392
1102	9	0.26031204032290134
1102	10	0.766728121735099
1102	11	0.55094541516997
1102	12	0.34924798179570615
1102	13	0.3770045971196012
1102	14	0.8245239394879107
1102	15	0.6201580497861627
1102	16	0.32539406172388396
1102	17	0.7564130168233802
1102	18	0.7899668048486959
1102	19	0.642524919940918
1102	20	0.8329671821585762
1102	21	0.7583512652594777
1102	22	0.5995605783383056
1102	23	0.6068246476782377
1102	24	0.7797487079293504
1102	25	0.635187005975105
1103	1	-0.41416725439306035
1103	2	-1.5204320373081792
1103	3	-0.4845048765630331
1103	4	-0.22807805305557594
1103	5	-0.26998029302306037
1103	6	-0.22042273712925464
1103	7	-0.1705340064503389
1103	8	0.009502827463016672
1103	9	0.5290131309932988
1103	10	0.7005164929794105
1103	11	0.31848137692180245
1103	12	0.14170115742719958
1103	13	-0.028198130903504648
1103	14	-1.1929839778533307
1103	15	-0.4337798630728713
1103	16	-0.2289342685192608
1103	17	0.17429788043447303
1103	18	0.35327602080535314
1103	19	0.3273133377218116
1103	20	0.3996231739877274
1103	21	0.37811179783385285
1103	22	0.03555992682623099
1103	23	0.8756305681104877
1103	24	0.5915105214842313
1103	25	0.3494853691122586
1104	1	-0.04601333312650979
1104	2	0.03889510231153892
1104	3	-0.3217361797966234
1104	4	-0.3309848057815401
1104	5	-0.25976336724148297
1104	6	-0.19816184082689148
1104	7	-0.13185839678681077
1104	8	-0.16296040553713453
1104	9	-2.127866220242751
1104	10	-0.6319313532974462
1104	11	-0.6043670709296194
1104	12	-0.03856080282334062
1104	13	-0.02626327618003188
1104	14	-1.7627624463468905
1104	15	-0.634440938972773
1104	16	-0.286703180776982
1104	17	0.004503008870514691
1104	18	0.18151953499915408
1104	19	0.24180818426304995
1104	20	0.25878512503967954
1104	21	0.4223737786686357
1104	22	0.48979182250013054
1104	23	0.8920296503104552
1104	24	0.5560052072110508
1104	25	0.4543402919953152
1105	1	-0.3513192667558549
1105	2	-0.22734228044095062
1105	3	-1.6621268568825585
1105	4	-0.7824327886093201
1105	5	-0.5838024025545536
1105	6	-0.30689671130634366
1105	7	-0.4125395839921282
1105	8	-0.42214891056783443
1105	9	0.11400441405516659
1105	10	0.2508624109525357
1105	11	-0.4002407231947487
1105	12	-0.3629267737852828
1105	13	-0.4675594082190102
1105	14	0.28248522716840085
1105	15	-0.06494916646452964
1105	16	-0.5464954088311202
1105	17	-0.039870309396739856
1105	18	-0.08105507999621153
1105	19	-0.12025160103236487
1105	20	-0.6405468144221516
1105	21	-0.23977269705448379
1105	22	-0.14848012239119157
1105	23	0.04148971407780522
1105	24	-0.008193719460379612
1105	25	0.11741775034600721
1106	1	-1.6999271106252642
1106	2	-1.1174262241039266
1106	3	-1.2014470216760031
1106	4	-1.460635174451447
1106	5	-0.9619989206972974
1106	6	-0.8113643420537997
1106	7	-0.6427614363714866
1106	8	-0.7329826395263552
1106	9	-0.4850681338243703
1106	10	-1.930209813314491
1106	11	-1.365361339978861
1106	12	-1.0234775196054358
1106	13	-1.0659754850040812
1106	14	-0.5178257608988304
1106	15	-0.9852923024120059
1106	16	-1.4720014089982598
1106	17	-0.797554595164371
1106	18	-0.6412310042922229
1106	19	-0.6209735058653933
1106	20	-0.38109421743174465
1106	21	-0.39026140528935116
1106	22	-0.4353083819426473
1106	23	-0.7527985250967291
1106	24	-0.8714283154159332
1106	25	-1.6648310365515968
1107	1	0.0163142106183507
1107	2	0.10064718793401184
1107	3	-0.08126467794247105
1107	4	0.15857201517724206
1107	5	-0.05863007328918712
1107	6	-0.008582464350901375
1107	7	-0.07234736835948041
1107	8	-0.22475794548545025
1107	9	0.34495476294893734
1107	10	-0.08035315367526863
1107	11	-0.015686507657072468
1107	12	-0.06655335670546947
1107	13	0.08498532808609127
1107	14	-1.152311295316217
1107	15	-0.5677393540599027
1107	16	-0.33602500223544496
1107	17	-0.09280154324989809
1107	18	0.16802125310910299
1107	19	0.22651601402814237
1107	20	0.3476592029525918
1107	21	0.3994721728531232
1107	22	-0.2658287479281327
1107	23	0.5717746814215587
1107	24	0.5447488725309171
1107	25	-4.700979862732879E-4
1108	1	-0.209905670365778
1108	2	0.1950586117207429
1108	3	-0.1604163208333702
1108	4	-0.42793943786208644
1108	5	-0.39591620787408033
1108	6	-0.35121567651813024
1108	7	-0.20955029527848978
1108	8	-0.40843950836963216
1108	9	0.09642269939150339
1108	10	0.23816514481396212
1108	11	-0.09329141018785236
1108	12	-0.3337073593223465
1108	13	-0.449727736603144
1108	14	-0.1152488735864381
1108	15	-0.27178764305119074
1108	16	-0.31355075928370013
1108	17	0.061126783245228467
1108	18	0.17852114319837398
1108	19	0.09441740958940414
1108	20	0.2317077773946039
1108	21	0.17181887788566996
1108	22	0.12094960153723378
1108	23	-0.07827913945835974
1108	24	-0.0014665144341350356
1108	25	-0.8337613862985762
1109	1	-0.8161616013640867
1109	2	-0.6846652927710117
1109	3	-0.7781491656770072
1109	4	-0.6615246373060231
1109	5	-1.0485731825673028
1109	6	-1.1473883235176459
1109	7	-0.7605033228973639
1109	8	-1.2791439220453285
1109	9	-0.9314386746674543
1109	10	-1.134455871669509
1109	11	-1.2774726598883888
1109	12	-1.158033657405035
1109	13	-1.2526615046164125
1109	14	-0.7346230875432553
1109	15	-1.3406199273622263
1109	16	-1.5256155072144608
1109	17	-1.4943820550074634
1109	18	-1.9244670669979769
1109	19	-2.184252027315061
1109	20	-1.7629657319009269
1109	21	-1.6365410682316792
1109	22	-1.907573053710621
1109	23	-1.2431475311886757
1109	24	-2.0822359789616627
1109	25	-1.3163010417853584
1110	1	0.13872671216430277
1110	2	0.20471496127904487
1110	3	0.06350397883873171
1110	4	0.3347179929489812
1110	5	0.25117719175513287
1110	6	0.24695129443052366
1110	7	0.16791063949113294
1110	8	0.18798812219378647
1110	9	0.4342199761068315
1110	10	0.39050356187770774
1110	11	0.23597321422352735
1110	12	0.2023467195259272
1110	13	0.3277645455385638
1110	14	-2.246487230679442
1110	15	-0.5653079450146237
1110	16	-0.034097224907107204
1110	17	0.0011541841635533956
1110	18	-2.7190940372175163
1110	19	-0.3472947906993369
1110	20	-1.8715346767950578
1110	21	-0.19328516534640272
1110	22	-1.558157301837845
1110	23	-0.31574629982582536
1110	24	0.27243977215823634
1110	25	0.29939544337300106
1111	1	-0.06238648119212442
1111	2	-1.2616586673016599
1111	3	-0.2221096838943879
1111	4	0.22487294114644385
1111	5	0.004768132134138625
1111	6	0.1707308390732507
1111	7	0.17057255686030004
1111	8	0.1293480331035255
1111	9	-1.4486476642711423
1111	10	-0.21520987806258773
1111	11	-0.05306560471450439
1111	12	0.02854043034197673
1111	13	0.07011491114963775
1111	14	0.9756309734446802
1111	15	0.3298775034731847
1111	16	0.42460147990233565
1111	17	-0.042951138254409216
1111	18	0.2086207048181521
1111	19	0.12352513185231118
1111	20	0.49422095060021576
1111	21	-0.32280076725624696
1111	22	-0.11795360261925225
1111	23	0.09023199822225081
1111	24	-0.2614248935231629
1111	25	-1.7942410252499528
1112	1	0.40847908499279484
1112	2	0.5228697309517436
1112	3	0.17477378155004786
1112	4	0.5062255757399328
1112	5	0.32941219624314755
1112	6	0.33591272053660504
1112	7	0.37146203084219565
1112	8	0.2005683572458068
1112	9	-0.10554750805161461
1112	10	0.6351194503392321
1112	11	0.33027620085491805
1112	12	0.2989997403070106
1112	13	0.3608680502744974
1112	14	0.6749517481694554
1112	15	0.41742724768253225
1112	16	0.48550545009590707
1112	17	-3.6628240074993457
1112	18	-0.8325438278267612
1112	19	-0.951106622970741
1112	20	0.11739325449160987
1112	21	-0.2863694240531558
1112	22	-1.4294932635240372
1112	23	-1.1232364900538627
1112	24	-0.04023182645989465
1112	25	0.3681505586864873
1113	1	-0.18528168529791955
1113	2	0.01675051950397588
1113	3	0.021487000115154586
1113	4	-0.2847694774029037
1113	5	0.02049629044925183
1113	6	-0.029024824924185566
1113	7	-0.1944444519693107
1113	8	-0.03952318309234215
1113	9	0.4021456583560763
1113	10	0.5194355669890669
1113	11	-0.0015739885551440125
1113	12	9.99988188532086E-4
1113	13	-0.30685079683793504
1113	14	-1.2739305360156123
1113	15	-1.440856386002288
1113	16	-0.19035064985309447
1113	17	-0.034345278524796186
1113	18	-0.4385665496176685
1113	19	0.05711656042198143
1113	20	0.4614559086083645
1113	21	-1.2618006696501283
1113	22	-1.7166924590247845
1113	23	-0.5294322749618843
1113	24	-2.294058541419446
1113	25	-0.3255655104521935
1114	1	0.21380293833546915
1114	2	0.2869284985604992
1114	3	0.09654330540654144
1114	4	0.5293031279920584
1114	5	0.18209988392258225
1114	6	0.3338690322065787
1114	7	0.3241849013724405
1114	8	0.19836160042568451
1114	9	0.5994392950669081
1114	10	0.5360909552678237
1114	11	0.42563546100096517
1114	12	0.4636373525810029
1114	13	0.31726955979880245
1114	14	1.1242651913696733
1114	15	0.5542472692873304
1114	16	0.49319361557313357
1114	17	-0.20598146007997542
1114	18	0.06791610869156983
1114	19	-0.3799433367651446
1114	20	-1.4106624594770807
1114	21	-0.6177176478944162
1114	22	-1.2706491392588717
1114	23	-2.6096650131694332
1114	24	-1.6500543341966818
1114	25	-1.1291603462554107
1115	1	0.5044438860963467
1115	2	0.5489752804302448
1115	3	0.4592453014803475
1115	4	0.7106115018310675
1115	5	0.5430374202876465
1115	6	0.4532709811073943
1115	7	0.5245813345530763
1115	8	0.6720508500600317
1115	9	0.855171968280807
1115	10	1.1197665595307953
1115	11	0.7640796370573426
1115	12	0.6600052610856082
1115	13	0.6580953939493881
1115	14	1.251508039418269
1115	15	0.7455751271024056
1115	16	0.8033252599531124
1115	17	0.8191571827794704
1115	18	1.0391959738117222
1115	19	0.4016979461295165
1115	20	0.8898922642490916
1115	21	-0.1463292441856936
1115	22	0.889009187819326
1115	23	0.9118949020350584
1115	24	1.0276097948707843
1115	25	1.1338777999683955
1116	1	0.7008831029507654
1116	2	0.7560355861033621
1116	3	0.633052310571312
1116	4	0.9795344997229805
1116	5	0.880602966621268
1116	6	0.734817412468999
1116	7	0.7710299103085205
1116	8	0.7720293842150312
1116	9	1.012745788556984
1116	10	0.9019204120861062
1116	11	0.7773518743959613
1116	12	0.8676711408009155
1116	13	0.3176384721800787
1116	14	1.3983273546863302
1116	15	1.0087807373617323
1116	16	1.060229555857191
1116	17	1.1926272390275017
1116	18	1.251949427304531
1116	19	1.3621568809699198
1116	20	1.1588454646886248
1116	21	0.6270469119529238
1116	22	0.5838941724996403
1116	23	-0.16823786862351398
1116	24	0.1930703040027737
1116	25	-0.23175411186193884
1201	1	0.33333555071845566
1201	2	0.6682438194091844
1201	3	0.5189627061311697
1201	4	0.2717069871396305
1201	5	0.3945775597244605
1201	6	0.28737962016705865
1201	7	0.3583735754174455
1201	8	0.3543605179956373
1201	9	0.6410388180063507
1201	10	0.9322055179976942
1201	11	0.6174564971853513
1201	12	0.6183420603130685
1201	13	0.5679873731795198
1201	14	1.0891752090729767
1201	15	0.7362495750267541
1201	16	0.5027180538213969
1201	17	0.6208272442476357
1201	18	0.6099120198732066
1201	19	0.8370551033940501
1201	20	0.9082603045189188
1201	21	0.9040871152831542
1201	22	0.9354599059531026
1201	23	0.4842776946523841
1201	24	0.8646301245119616
1201	25	1.0358079400740579
1202	1	0.22795454269031978
1202	2	0.6555728230070876
1202	3	0.3405343491163535
1202	4	0.11620825540178714
1202	5	0.09838384584185889
1202	6	0.051871924262719915
1202	7	0.05628886613657613
1202	8	0.09786634352681034
1202	9	0.5509629239735929
1202	10	-0.1321748618935885
1202	11	0.20930184339983526
1202	12	0.20230519435005334
1202	13	0.2478836545994767
1202	14	0.805336432356782
1202	15	0.6281326420028037
1202	16	0.37373452677064023
1202	17	-1.675566567826941
1202	18	-0.0791243749508439
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2210	17	0.6107930019664778
2210	18	0.6117211189386325
2210	19	0.7390624220261728
2210	20	0.813166542013029
2210	21	0.9064209545996107
2210	22	0.989687776967628
2210	23	1.2897860837132953
2210	24	1.0072710976933976
2210	25	0.9221320178203101
2211	1	0.12008261603023129
2211	2	-0.5873911401849767
2211	3	-0.028752058511997802
2211	4	0.35273483559376473
2211	5	0.22539359232644074
2211	6	0.21685761257921143
2211	7	0.22269821181241412
2211	8	0.3274722408973716
2211	9	0.7352137480498584
2211	10	0.7750025484576284
2211	11	0.4141551305619018
2211	12	0.25330890385236415
2211	13	0.28165900512166786
2211	14	-0.8733438327274214
2211	15	0.039091233630770716
2211	16	0.2775830844192555
2211	17	0.4053863377025948
2211	18	0.34071341360566976
2211	19	0.6438788634671866
2211	20	0.7700067210418453
2211	21	0.6463955559590531
2211	22	0.11293402415159193
2211	23	0.5147361075308865
2211	24	-0.08456535294850909
2211	25	0.4052778680103728
2212	1	-0.08054798136954133
2212	2	-1.1573104019721896
2212	3	-0.3144517007763241
2212	4	0.1600703475530949
2212	5	0.16031590510457505
2212	6	0.15577568191418578
2212	7	0.13334965045243763
2212	8	0.09604721220382828
2212	9	-2.17821241568344
2212	10	-0.10186735840712657
2212	11	0.013798309425169464
2212	12	-0.040311629087916856
2212	13	-0.12646272763794997
2212	14	0.3364012507569356
2212	15	0.09358381141579741
2212	16	0.21558357013904583
2212	17	0.30000495875631117
2212	18	-0.011123252446225251
2212	19	-0.6529030397109779
2212	20	0.26307467151265485
2212	21	0.19849078142500076
2212	22	-1.8238672773335622
2212	23	-0.4783025016033875
2212	24	0.1068497784422533
2212	25	-0.5257584324306848
2213	1	0.2541290108121643
2213	2	0.48194673790469267
2213	3	0.29143100689251256
2213	4	0.5115129913977938
2213	5	0.4892697499545591
2213	6	0.3868248359510318
2213	7	0.18712792188755145
2213	8	0.3911914602900026
2213	9	0.8336804523443533
2213	10	0.7527355254137191
2213	11	0.19837327426159135
2213	12	0.26327228514391976
2213	13	0.29142970685511926
2213	14	-1.5256022827140612
2213	15	-0.279553496855101
2213	16	0.05824672832543598
2213	17	-1.9065172941248179
2213	18	-0.7605088066128047
2213	19	-0.29325420283901593
2213	20	0.42708036939762195
2213	21	0.073204950024369
2213	22	0.6165367737713794
2213	23	1.0458034960072808
2213	24	0.7789883270136286
2213	25	-0.6840654304308842
2214	1	0.4352705947847859
2214	2	0.36227284339067367
2214	3	0.2876577568153742
2214	4	0.7605529138645721
2214	5	0.6384633399620725
2214	6	0.5758708835781619
2214	7	0.4824065221198026
2214	8	0.39280779355514284
2214	9	0.883813069054014
2214	10	0.826949017063491
2214	11	0.20103762925829471
2214	12	0.23556353171021271
2214	13	0.3206168267853613
2214	14	1.1505456671555336
2214	15	0.6177437072214076
2214	16	0.7070853680474656
2214	17	0.7166751697097098
2214	18	0.6323096384158049
2214	19	-0.6287176759767448
2214	20	0.373044677933116
2214	21	0.749127517842382
2214	22	0.9223843529322195
2214	23	1.4089652404380786
2214	24	1.071561925905634
2214	25	1.2574547389707647
2215	1	-0.13125688077041886
2215	2	0.2335247707005692
2215	3	0.2831475439752502
2215	4	0.568238981400718
2215	5	0.46104919532374566
2215	6	0.4126569981333219
2215	7	0.3318049288196079
2215	8	0.38922433043317994
2215	9	0.4600921934177933
2215	10	0.7810074146029891
2215	11	0.48477284748118166
2215	12	0.3744627758990404
2215	13	0.23275955074138466
2215	14	1.1607379951817904
2215	15	0.6704625061071923
2215	16	0.592879444868835
2215	17	0.7761836046143102
2215	18	0.5926475163031952
2215	19	-0.02805866865895723
2215	20	-1.191100350788446
2215	21	-1.4851103592112977
2215	22	0.07130428522377485
2215	23	0.7030568834529731
2215	24	0.8100369402541787
2215	25	0.13834338118515888
2216	1	0.14513811024492185
2216	2	-0.18537174243300925
2216	3	0.12690616171752112
2216	4	0.6090193139946578
2216	5	0.5762439524332805
2216	6	0.3278125187095995
2216	7	0.37342289526964123
2216	8	0.3219158111967815
2216	9	0.6995810235736144
2216	10	-1.2174237491665352
2216	11	-0.17368352689601352
2216	12	0.16604970120440754
2216	13	0.3015076092304696
2216	14	-0.013839146125853574
2216	15	-0.059613800165699615
2216	16	0.3697349371050649
2216	17	0.7197493889097117
2216	18	0.5180551028181739
2216	19	-1.0265037162261024
2216	20	-0.6332208054321526
2216	21	-0.5142231036097229
2216	22	-0.26412056655023974
2216	23	-4.182258375149864
2216	24	-1.7138542131413246
2216	25	-0.7847197188325978
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	13.0
4	27.0
5	41.0
6	77.0
7	304.0
8	213.0
9	639.0
10	1608.0
11	3332.0
12	6230.0
13	9878.0
14	14023.0
15	17574.0
16	20834.0
17	22670.0
18	23762.0
19	24584.0
20	25267.0
21	26290.0
22	27021.0
23	28774.0
24	31424.0
25	34404.0
26	38500.0
27	44491.0
28	52168.0
29	63423.0
30	77257.0
31	95690.0
32	124998.0
33	165787.0
34	221640.0
35	301525.0
36	422799.0
37	621850.0
38	1020222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.52805570065022	21.384990959270596	13.88103134689625	23.205921993182933
2	15.433570213461117	21.17257764827098	40.90868715206684	22.485164986201063
3	17.896056335186287	26.56013021122332	30.212372308144143	25.33144114544625
4	12.927901366324823	17.517751684924686	38.47690319137964	31.077443757370844
5	13.421054696828197	37.45393535309596	35.557058499719155	13.567951450356688
6	30.409098309184362	39.19544961248347	17.60680417322475	12.788647905107418
7	25.76440493166822	32.87824583299591	23.801108025323572	17.5562412100123
8	23.62584207756278	37.272865358951066	21.408962020122985	17.692330543363177
9	24.3280198575033	17.510032304595313	22.40874519333573	35.753202644565654
10	15.054708960993507	30.042220908919465	33.81853611951541	21.08453401057162
11	31.812470366430645	25.995856064079128	23.843239959334056	18.348433610156174
12	20.812092420353057	27.47728145829066	31.801699235677393	19.90892688567889
13	26.08349021469441	24.419324122029508	27.233240502909617	22.26394516036647
14	20.96792907481011	23.492707994105636	28.281262523982992	27.258100407101267
15	22.535204557346148	31.070363713866055	24.4354813622602	21.958950366527596
16	22.004336235646413	31.406799376662626	25.836246995614164	20.7526173920768
17	20.14367784687235	30.40162935027472	28.720381085929002	20.73431171692393
18	20.255493680194057	30.160819893377095	29.44168695550803	20.141999470920823
19	20.96304746276879	28.999053720486913	29.555304671459993	20.48259414528431
20	22.111627688024406	28.688250814281137	28.881033133900768	20.319088363793686
21	22.581744260494467	29.066590452813955	27.878242766338694	20.473422520352884
22	21.47527299087709	28.654395749275157	29.388610839381972	20.481720420465777
23	21.857553855633807	28.883886701482712	28.617400195759696	20.64115924712378
24	21.433385088686034	28.156312934102573	28.972545564745612	21.437756412465784
25	22.152022102800682	28.49089936646005	28.279261291687806	21.07781723905146
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2478.0
1	2478.0
2	2832.5
3	3187.0
4	3187.0
5	3187.0
6	4698.5
7	6210.0
8	6210.0
9	6210.0
10	9927.0
11	13644.0
12	13644.0
13	13644.0
14	22854.0
15	32064.0
16	32064.0
17	32064.0
18	51832.0
19	71600.0
20	71600.0
21	71600.0
22	103223.5
23	134847.0
24	134847.0
25	134847.0
26	176821.0
27	218795.0
28	218795.0
29	218795.0
30	264526.5
31	310258.0
32	310258.0
33	310258.0
34	348391.5
35	386525.0
36	386525.0
37	386525.0
38	412882.0
39	439239.0
40	439239.0
41	439239.0
42	438844.0
43	438449.0
44	438449.0
45	438449.0
46	418587.5
47	398726.0
48	398726.0
49	398726.0
50	375946.5
51	353167.0
52	353167.0
53	353167.0
54	319045.5
55	284924.0
56	284924.0
57	284924.0
58	246350.0
59	207776.0
60	207776.0
61	207776.0
62	171008.0
63	134240.0
64	134240.0
65	134240.0
66	103850.5
67	73461.0
68	73461.0
69	73461.0
70	54401.0
71	35341.0
72	35341.0
73	35341.0
74	25244.0
75	15147.0
76	15147.0
77	15147.0
78	10600.5
79	6054.0
80	6054.0
81	6054.0
82	4145.0
83	2236.0
84	2236.0
85	2236.0
86	1488.0
87	740.0
88	740.0
89	740.0
90	457.0
91	174.0
92	174.0
93	174.0
94	110.5
95	47.0
96	47.0
97	47.0
98	30.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07578427620553031
2	0.016501640918690337
3	0.027624139127043586
4	0.03446013298809696
5	0.042865043472998667
6	0.016557673655256348
7	0.01823865575223669
8	0.03036974321877814
9	0.031350316108683336
10	0.02047996521487714
11	0.02112434168538627
12	0.015717182606766175
13	0.02319755293832869
14	0.03328344552021073
15	0.03151841431838137
16	0.02266524194095158
17	0.022497143731253548
18	0.024598371352478974
19	0.017622295650010562
20	0.015409002555653115
21	0.012243152939673475
22	0.017734361123142584
23	0.019079146800726858
24	0.017622295650010562
25	0.10705054320936465
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3569342.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.89297992601483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.39523480633305	73.20842169476414
2	7.88465404649536	12.91395631106432
3	1.4670544963383487	3.604243932570186
4	0.45738334233535766	1.4982593948945206
5	0.22778348980272375	0.9326934378946029
6	0.13456846491575217	0.6612127557612194
7	0.08968203211776997	0.5141030199161327
8	0.060227589058033264	0.3945773393377431
9	0.046668716981199794	0.3439656272622836
>10	0.21535101328327744	3.065088692618533
>50	0.01408016955897024	0.796381014190228
>100	0.00635093284314117	0.9621914073433671
>500	5.49085682794653E-4	0.3016112068479172
>1k	4.1181425417500823E-4	0.8032941655348365
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	4476	0.12540126443473334	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4020	0.11262580049768278	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.6032736566011324E-5	0.0	0.0	0.0
3	0.0	5.6032736566011324E-5	0.0	0.0	0.0
4	0.0	5.6032736566011324E-5	0.0	0.0	0.0
5	0.0	5.6032736566011324E-5	0.0	0.0	0.0
6	0.0	5.6032736566011324E-5	0.0	0.0	0.0
7	0.0	5.6032736566011324E-5	0.0	2.8016368283005662E-5	0.0
8	0.0	5.6032736566011324E-5	0.0	2.8016368283005662E-5	0.0
9	5.6032736566011324E-5	5.6032736566011324E-5	0.0	5.6032736566011324E-5	0.0
10	5.6032736566011324E-5	5.6032736566011324E-5	0.0	5.6032736566011324E-5	0.0
11	5.6032736566011324E-5	5.6032736566011324E-5	0.0	5.6032736566011324E-5	0.0
12	5.6032736566011324E-5	5.6032736566011324E-5	0.0	1.1206547313202265E-4	0.0
13	5.6032736566011324E-5	5.6032736566011324E-5	0.0	1.1206547313202265E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAGC	155	3.092282E-10	11.041698	1
GTAGGAC	210	0.0	10.40622	3
GTATTAG	690	0.0	9.645927	1
GTACTAG	300	0.0	9.508129	1
TACACCG	90	0.001112688	9.501465	5
TGCACCG	120	1.6986976E-5	9.501465	5
GTCCTAA	300	0.0	9.191191	1
TACACGG	115	1.10949724E-4	9.088358	5
TCTAGAC	335	0.0	8.792277	3
CTAGACT	265	9.094947E-12	8.6051	4
GTCTTAC	300	0.0	8.557316	1
CTAGACA	490	0.0	8.531927	4
GCGTCAG	180	3.985624E-7	8.45167	1
CTAGGAC	270	1.4551915E-11	8.445628	3
CCAGGAC	580	0.0	8.354619	3
GTGGTAT	1820	0.0	8.306552	1
GTCCTAC	280	3.274181E-11	8.149824	1
TAGACTG	385	0.0	8.144113	5
GGTATCA	3035	0.0	8.051365	1
CTTAGAC	320	1.8189894E-12	8.016748	3
>>END_MODULE