FastQCFastQC Report
Wed 25 May 2016
SRR1295309_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295309_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3569342
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT109000.30537841428476176No Hit
GGTATCAACGCAGAGTACTTTTTTT89240.2500180705575425No Hit
TATCAACGCAGAGTACTTTTTTTTT62830.1760268419221246No Hit
GTGGTATCAACGCAGAGTACATGGG51890.14537693502051638No Hit
ACGCAGAGTACTTTTTTTTTTTTTT42140.11806097594458587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTCG957.431845E-812.9953419
AGCGTCA2550.012.3029833
TAACCCG1354.6820787E-911.2673795
CAGCGTC2800.011.2045012
TGTACCG951.3506198E-511.0079325
GGTATCA40250.010.0260581
GCAGCGT3050.09.98574351
CTAGAAC2800.09.506853
GTACTAG2252.5465852E-119.3061581
GCGTCAG3400.09.2272384
AGGACCG950.00181273589.006495
AACGCTG950.00181273589.006495
GGACCGT850.00745973978.9380986
GGTGCGT850.0074605978.9379738
TGCGTTT850.0074605978.93797312
GTATTAG5250.08.7018621
GTGGTAT20400.08.6312131
TACACTG8500.08.6120885
TCTGTCG2106.4283086E-98.5921588
ATAGGAC3450.08.5423883
Produced by FastQC (version 0.11.5)