FastQCFastQC Report
Wed 25 May 2016
SRR1295308_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295308_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5988807
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG78360.13084408964924066No Hit
GTATCAACGCAGAGTACTTTTTTTT66530.11109057279688592No Hit
ATTCCATTCCATTCCATTCCATTCC64040.10693281650251878No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACG1054.101611E-59.9541284
TATACCG1201.6988803E-59.5016675
ACTGTCG1002.761722E-49.49865158
CTGTCCG800.00452454439.4984929
CTAGGAC5750.09.2537193
AATCGGT850.00745319068.93923517
ACCGCTC1201.7681232E-48.7070978
GTCTTAG5050.08.4721411
TCTAGAC5850.08.4458553
TGTACTG8450.08.4334325
TATACTG16050.08.4064595
GGTATCA56400.08.395031
TAGACAG10450.08.3651045
CGCAGAT1951.5421392E-78.2808768
CGTAGAC1150.00110956778.262253
CTGTGCG1856.1417995E-78.2149129
GTATTAA10650.08.213161
GTCCTGG11500.08.1848251
GTCTTAC7000.08.1493921
CTAGAAC7000.08.14421753
Produced by FastQC (version 0.11.5)