##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295308_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5988807 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.17954126756798 31.0 30.0 33.0 26.0 34.0 2 30.149141723885908 31.0 30.0 34.0 26.0 34.0 3 30.47650091245218 31.0 30.0 34.0 26.0 34.0 4 33.86269786286317 35.0 35.0 37.0 28.0 37.0 5 34.030353290730524 35.0 35.0 37.0 28.0 37.0 6 34.281738917283526 36.0 35.0 37.0 30.0 37.0 7 34.24743592505152 37.0 35.0 37.0 30.0 37.0 8 34.31179832644465 37.0 35.0 37.0 30.0 37.0 9 35.28655673826189 39.0 35.0 39.0 28.0 39.0 10 35.21613469928151 39.0 35.0 39.0 27.0 39.0 11 35.595170624132656 39.0 35.0 39.0 29.0 39.0 12 35.58992300135903 39.0 35.0 39.0 29.0 39.0 13 35.50081643973499 39.0 35.0 39.0 29.0 39.0 14 35.70759952691746 39.0 34.0 41.0 26.0 41.0 15 36.325265783318784 39.0 36.0 41.0 27.0 41.0 16 36.42639627558544 39.0 36.0 41.0 28.0 41.0 17 36.23393924699861 39.0 36.0 41.0 27.0 41.0 18 36.20458515360405 39.0 36.0 41.0 27.0 41.0 19 36.26543567024284 39.0 35.0 41.0 27.0 41.0 20 36.24045974431969 39.0 35.0 41.0 27.0 41.0 21 36.23078886997026 39.0 35.0 41.0 27.0 41.0 22 35.97635472306922 39.0 34.0 41.0 27.0 41.0 23 35.65398868255397 39.0 34.0 41.0 26.0 41.0 24 35.98321452002043 39.0 34.0 41.0 27.0 41.0 25 34.674831230994755 38.0 32.0 40.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30131781575704153 1101 2 0.5289358265043518 1101 3 0.3815622904017779 1101 4 0.34898809013833443 1101 5 0.2035314426821202 1101 6 0.23021955714197873 1101 7 0.26975524397088435 1101 8 0.3166019035756875 1101 9 0.28651721752551396 1101 10 0.597220667666825 1101 11 0.46925460261711294 1101 12 0.5716125451372847 1101 13 0.4627465076552042 1101 14 1.0341477439499016 1101 15 0.7326504795637518 1101 16 0.5659750927294951 1101 17 0.8037450122400216 1101 18 0.808227402515648 1101 19 0.7987048203833069 1101 20 0.8451695690547538 1101 21 0.9091646995361629 1101 22 0.7075627843847059 1101 23 -1.6037920547222626 1101 24 0.06471516291438917 1101 25 0.5381090059111386 1102 1 0.23579683359694315 1102 2 0.3888264911244512 1102 3 0.280719086233983 1102 4 0.3286120974236084 1102 5 0.08262035742127694 1102 6 0.0900032273869229 1102 7 0.09586122793299268 1102 8 0.21529995096856425 1102 9 0.3601888783356486 1102 10 0.8399993518129918 1102 11 0.4924074211546525 1102 12 0.36808993634089404 1102 13 0.35969101377560264 1102 14 0.8808225920661528 1102 15 0.6441755838229355 1102 16 0.3797222207752142 1102 17 0.7806734450700787 1102 18 0.7341854848546561 1102 19 0.7576282317179519 1102 20 0.7976799547493627 1102 21 0.7601681941494363 1102 22 0.7164670493574832 1102 23 0.7559618501701948 1102 24 0.7615960757614388 1102 25 0.6316719668718704 1103 1 -0.4840542444604665 1103 2 -1.699813423605594 1103 3 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20 0.6505987326526395 2211 21 0.6310342526866108 2211 22 0.023733097043319162 2211 23 0.6060332451716874 2211 24 -0.1061693136588886 2211 25 0.4000261727610308 2212 1 0.02434932768482767 2212 2 -1.0676025909852136 2212 3 -0.3435625229614203 2212 4 0.10553628485142497 2212 5 0.19471338387455717 2212 6 0.1663107673968227 2212 7 0.17134038428783072 2212 8 0.1222769349646029 2212 9 -2.33200987874514 2212 10 -0.0949123789245121 2212 11 -0.007376030420992663 2212 12 0.02421103846926087 2212 13 0.0726474841050333 2212 14 0.6731492158356076 2212 15 0.26666980919828376 2212 16 0.3484430039243094 2212 17 0.39603532154811205 2212 18 0.07883433267954842 2212 19 -0.5483134445730897 2212 20 0.3369199041526514 2212 21 0.36557228038383016 2212 22 -1.714137773829009 2212 23 -0.3415021271712746 2212 24 0.27556173049388377 2212 25 -0.5020518410806147 2213 1 0.14872459979184782 2213 2 0.42080298195485 2213 3 0.19432738854239417 2213 4 0.3245060205870516 2213 5 0.4061497573672028 2213 6 0.2269066264420161 2213 7 0.18462024653222642 2213 8 0.2573326331327479 2213 9 0.5902227202955359 2213 10 0.5900430182818965 2213 11 0.04003244745275936 2213 12 0.026875058380191774 2213 13 0.06412786634653145 2213 14 -1.5172782201480217 2213 15 -0.340474536139304 2213 16 -0.05979036588352926 2213 17 -2.163254031443792 2213 18 -0.8553403312261096 2213 19 -0.523446845676176 2213 20 0.1419031979196248 2213 21 -0.2416273103295481 2213 22 0.28649040689995786 2213 23 0.8241919579951187 2213 24 0.6129486736573782 2213 25 -0.9528180293097748 2214 1 0.25373561598054906 2214 2 0.16677450475122413 2214 3 0.05709338142733955 2214 4 0.3771328993501797 2214 5 0.4557002592806185 2214 6 0.30118938080254765 2214 7 0.24190858939731896 2214 8 0.17833467484697252 2214 9 0.6500620705286266 2214 10 0.5626903888681625 2214 11 0.02047106626557138 2214 12 -0.06054522050174427 2214 13 0.05184600789042548 2214 14 0.8836050417345902 2214 15 0.4310029082326139 2214 16 0.4225794565681156 2214 17 0.34899023003906393 2214 18 0.34979046509982936 2214 19 -0.848908602922414 2214 20 0.1650168992429215 2214 21 0.4715483736469963 2214 22 0.6845741052305101 2214 23 1.1980323165529967 2214 24 0.9126975121940433 2214 25 0.8648772878214075 2215 1 -0.05739293502758258 2215 2 0.32834530113314386 2215 3 0.2684438753273817 2215 4 0.4683367306742596 2215 5 0.5010669543679569 2215 6 0.3518220353904198 2215 7 0.2609982552202439 2215 8 0.2198306479444767 2215 9 0.3602367656813925 2215 10 0.6923270901688454 2215 11 0.4298485184001777 2215 12 0.3396944552881038 2215 13 0.2566881913684398 2215 14 1.091808652758992 2215 15 0.49662117166143105 2215 16 0.488680597569207 2215 17 0.6790265513186 2215 18 0.5820343272385387 2215 19 -0.21802388927371652 2215 20 -1.3599662647975421 2215 21 -1.7649178271781523 2215 22 -0.09628122608243928 2215 23 0.6084807421458436 2215 24 0.7344062213002758 2215 25 -0.02107773859237483 2216 1 -0.008814767727589157 2216 2 -0.3217801719112927 2216 3 -0.12361353135488429 2216 4 0.22488715210497645 2216 5 0.32905908644703885 2216 6 0.06667546804411018 2216 7 0.06841840193720117 2216 8 -0.039963989952937595 2216 9 0.2474278476636158 2216 10 -1.5698370303945524 2216 11 -0.5322462635082843 2216 12 -0.2653098962653857 2216 13 -0.013768468069486062 2216 14 -0.5008158218348342 2216 15 -0.4361347731850671 2216 16 -0.03636860945936604 2216 17 0.3503546922751113 2216 18 0.12699790818328438 2216 19 -1.3353608946503925 2216 20 -1.0065304889136044 2216 21 -0.8372289378845394 2216 22 -0.6661248953851882 2216 23 -4.379690967943127 2216 24 -2.0434017665648128 2216 25 -1.114091836584933 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 16.0 4 38.0 5 86.0 6 91.0 7 464.0 8 229.0 9 619.0 10 1821.0 11 4077.0 12 7862.0 13 12943.0 14 18681.0 15 24628.0 16 29087.0 17 32647.0 18 34487.0 19 36067.0 20 37540.0 21 38944.0 22 41311.0 23 44484.0 24 48622.0 25 53436.0 26 60736.0 27 70428.0 28 82899.0 29 102317.0 30 125555.0 31 156545.0 32 205890.0 33 275477.0 34 373226.0 35 515185.0 36 727078.0 37 1076175.0 38 1749116.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.704535181079535 21.433041620579125 14.105553014555447 22.75687018378589 2 15.272000347369511 21.155037024913742 41.28176009459139 22.291202533125357 3 18.01164513553116 26.5861574176066 30.355151413318467 25.047046033543772 4 12.335414557881762 17.602666506315497 39.26547472297665 30.796444212826092 5 13.233803237573452 37.59302966375267 35.667430676582256 13.50573642209162 6 30.13416532076045 38.91669398559865 18.31588679257257 12.633253901068336 7 25.466775311048544 32.80207477590426 24.488207636582203 17.242942276464994 8 22.88177903952057 37.754162044678104 21.802885390329127 17.561173525472203 9 23.976923111614962 17.668578827744984 22.74906548089629 35.60543257974377 10 14.675459260490195 30.19602963784412 34.176746500902276 20.951764600763408 11 31.39792061264985 26.288586016267875 24.115839508426458 18.19765386265582 12 20.572287924220447 27.67336434590119 32.172808641434095 19.58153908844427 13 25.807934805718364 24.631587807905078 27.59784144317538 21.962635943201185 14 20.70076264701672 23.57034795258583 28.686883096035864 27.04200630436159 15 22.26923657685611 31.25712397758812 24.661389745213825 21.81224970034195 16 21.608840104543926 31.73388162833729 26.091505831034528 20.565772436084256 17 19.67243259366841 30.731481807776863 29.07831291395401 20.517772684600715 18 19.82132045486621 30.454340094083182 29.90280186830192 19.82153758274869 19 20.578699733441464 29.231422610262896 29.91380978966743 20.27606786662821 20 21.85549587132254 28.870381488294495 29.211313636701437 20.062809003681533 21 22.313774247068558 29.37211249988519 28.07029614593623 20.243817107110022 22 20.981065952851598 28.97195465662124 29.818051308909617 20.228928081617546 23 21.367609154893703 29.154072015767092 28.99851878721925 20.47980004211995 24 20.90937375840651 28.375944362583127 29.34882758503209 21.365854293978277 25 21.731562467194934 28.8059223965065 28.61288653141898 20.849628604879587 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3048.0 1 3048.0 2 3818.0 3 4588.0 4 4588.0 5 4588.0 6 7093.5 7 9599.0 8 9599.0 9 9599.0 10 16171.5 11 22744.0 12 22744.0 13 22744.0 14 39601.0 15 56458.0 16 56458.0 17 56458.0 18 91094.0 19 125730.0 20 125730.0 21 125730.0 22 181318.0 23 236906.0 24 236906.0 25 236906.0 26 308744.0 27 380582.0 28 380582.0 29 380582.0 30 458977.5 31 537373.0 32 537373.0 33 537373.0 34 601953.0 35 666533.0 36 666533.0 37 666533.0 38 710839.0 39 755145.0 40 755145.0 41 755145.0 42 755587.5 43 756030.0 44 756030.0 45 756030.0 46 716425.5 47 676821.0 48 676821.0 49 676821.0 50 634006.0 51 591191.0 52 591191.0 53 591191.0 54 526113.5 55 461036.0 56 461036.0 57 461036.0 58 393409.0 59 325782.0 60 325782.0 61 325782.0 62 264080.5 63 202379.0 64 202379.0 65 202379.0 66 153808.5 67 105238.0 68 105238.0 69 105238.0 70 75336.0 71 45434.0 72 45434.0 73 45434.0 74 31538.0 75 17642.0 76 17642.0 77 17642.0 78 11808.0 79 5974.0 80 5974.0 81 5974.0 82 3950.5 83 1927.0 84 1927.0 85 1927.0 86 1197.5 87 468.0 88 468.0 89 468.0 90 298.0 91 128.0 92 128.0 93 128.0 94 76.5 95 25.0 96 25.0 97 25.0 98 25.5 99 26.0 100 26.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07888048487787301 2 0.01581283217174973 3 0.02648273687898107 4 0.03251064861499127 5 0.0402417376282121 6 0.014961243533144415 7 0.01796685049292789 8 0.03382977611400735 9 0.034030149911326246 10 0.01990379720034391 11 0.019670027770138526 12 0.015228408596236278 13 0.021506787578895094 14 0.036033887884515226 15 0.03311177000694796 16 0.0213064137815762 17 0.0213064137815762 18 0.02591501111991086 19 0.0167646077090145 20 0.013558626951912126 21 0.011888845307587971 22 0.01795015267648465 23 0.0191356976439548 24 0.01733233346808471 25 0.10851910906462672 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 5988807.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.20033490927386 #Duplication Level Percentage of deduplicated Percentage of total 1 80.89646622776343 58.40751954621282 2 13.282354920080339 19.17980947227284 3 3.3527648440211215 7.262122338310931 4 1.166678012948658 3.3693817306471705 5 0.4843720771175339 1.7485913094293286 6 0.24175125207989492 1.0472712818942822 7 0.1350098435834509 0.6823429145941683 8 0.07378885317831575 0.42620639296365076 9 0.0639406201212309 0.4154880768353574 >10 0.27151305916458973 3.5284950742585197 >50 0.01931075376411873 0.966662817747672 >100 0.01060947497176573 1.4012149646822583 >500 8.826268968734865E-4 0.44021000717817604 >1k 4.413021611381792E-4 0.5958560022308215 >5k 1.1613214743903827E-4 0.5288280707421175 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGGTATCAACGCAGAGTACATGGG 7836 0.13084408964924066 No Hit GTATCAACGCAGAGTACTTTTTTTT 6653 0.11109057279688592 No Hit ATTCCATTCCATTCCATTCCATTCC 6404 0.10693281650251878 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.001868986594492E-4 0.0 10 0.0 0.0 0.0 1.001868986594492E-4 0.0 11 0.0 0.0 0.0 1.502803479891738E-4 0.0 12 0.0 0.0 0.0 1.6697816443241533E-4 0.0 13 0.0 0.0 0.0 1.8367598087565688E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGACG 105 4.101611E-5 9.954128 4 TATACCG 120 1.6988803E-5 9.501667 5 ACTGTCG 100 2.761722E-4 9.4986515 8 CTGTCCG 80 0.0045245443 9.498492 9 CTAGGAC 575 0.0 9.253719 3 AATCGGT 85 0.0074531906 8.939235 17 ACCGCTC 120 1.7681232E-4 8.707097 8 GTCTTAG 505 0.0 8.472141 1 TCTAGAC 585 0.0 8.445855 3 TGTACTG 845 0.0 8.433432 5 TATACTG 1605 0.0 8.406459 5 GGTATCA 5640 0.0 8.39503 1 TAGACAG 1045 0.0 8.365104 5 CGCAGAT 195 1.5421392E-7 8.280876 8 CGTAGAC 115 0.0011095677 8.26225 3 CTGTGCG 185 6.1417995E-7 8.214912 9 GTATTAA 1065 0.0 8.21316 1 GTCCTGG 1150 0.0 8.184825 1 GTCTTAC 700 0.0 8.149392 1 CTAGAAC 700 0.0 8.1442175 3 >>END_MODULE