FastQCFastQC Report
Wed 25 May 2016
SRR1295308_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295308_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5988807
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT190190.31757577093401074No Hit
GGTATCAACGCAGAGTACTTTTTTT149740.2500331034210987No Hit
TATCAACGCAGAGTACTTTTTTTTT108520.18120470404205713No Hit
GTGGTATCAACGCAGAGTACATGGG86660.14470327729713114No Hit
ACGCAGAGTACTTTTTTTTTTTTTT70120.11708508890000964No Hit
GTACATAAGCAGTGGTATCAACGCA60110.10037057464032485No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATCG657.9779775E-411.7012475
TATACCG1201.264234E-711.0918075
AGCGTCA3750.010.6482243
GCAGCGT4150.09.8616151
ATACCGT1856.548362E-109.7532936
CAGCGTC4200.09.7337082
GGTATCA72100.09.1479891
TAGACTG9200.08.9905645
ACACCGC1606.7857945E-78.9030886
GTCCTAG5700.08.8497041
GTCTTAG6500.08.7854821
ACCGTTC1201.7726966E-48.7050238
TATTCCG2303.9835868E-108.6805455
GAACCGT1107.175128E-48.6332976
AAGGCGT1107.175128E-48.6332976
GTACTAG4200.08.6111671
CCTAGAC6700.08.3721383
GTCTTAC5850.08.29739951
ACAGTGC11750.08.2436748
TATACTG14150.08.1970755
Produced by FastQC (version 0.11.5)