FastQCFastQC Report
Wed 25 May 2016
SRR1295307_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295307_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299987
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA686465.280514343604974No Hit
GCTTATGTACTCTGCGTTGATACCA412503.1731086541634648No Hit
CTTATGTACTCTGCGTTGATACCAC285642.1972527417581866No Hit
GAGTACATAAGCAGTGGTATCAACG204011.5693233855415478No Hit
TATGTACTCTGCGTTGATACCACTG188551.4503991193758092No Hit
GCGTTGATACCACTGCTTATGTACT147091.1314728531900704No Hit
CATAAGCAGTGGTATCAACGCAGAG124870.9605480670191316No Hit
CCCATGTACTCTGCGTTGATACCAC116850.8988551423975779No Hit
CTGCTTATGTACTCTGCGTTGATAC112390.8645471070095316No Hit
ACGCAGAGTACATAAGCAGTGGTAT110860.8527777585468163No Hit
ACTCTGCGTTGATACCACTGCTTAT106430.8187004946972547No Hit
GTATCAACGCAGAGTACATAAGCAG93640.7203148954566468No Hit
CATGTACTCTGCGTTGATACCACTG81960.6304678431399698No Hit
TATCAACGCAGAGTACATAAGCAGT80580.6198523523696775No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA78430.6033137254449468No Hit
GGTATCAACGCAGAGTACATAAGCA78070.6005444669831315No Hit
GTGGTATCAACGCAGAGTACATAAG62920.4840048400484005No Hit
AAGCAGTGGTATCAACGCAGAGTAC58890.45300453004530045No Hit
GTATCAACGCAGAGTACTTTTTTTT51600.39692704619353886No Hit
GTACTGGTTCACTATCGGTCAGTCA51360.39508087388566193No Hit
ACATAAGCAGTGGTATCAACGCAGA49910.38392691619223884No Hit
GTGGTATCAACGCAGAGTACATGGG47510.3654651931134696No Hit
ATCATGTACTCTGCGTTGATACCAC45030.34638807926540804No Hit
GTACATGATAAGCAGTGGTATCAAC44790.3445419069575311No Hit
CCACTGCTTATGTACTCTGCGTTGA44000.3384649231107696No Hit
GTACATGGGAAGCAGTGGTATCAAC42720.32861867080209267No Hit
GATACCACTGCTTATGTACTCTGCG42400.3261571077249234No Hit
GTTGATACCACTGCTTATGTACTCT40580.31215696772352336No Hit
GGATACCACGTGTCCCGCCCTACTC39260.3020030200302003No Hit
ATACAGGGTGACAGCCCCGTACACA38360.29507987387566187No Hit
GCAGTGGTATCAACGCAGAGTACAT38180.2936952446447541No Hit
GTACTCTGCGTTGATACCACTGCTT38140.293387549260108No Hit
ATACCACTGCTTATGTACTCTGCGT36860.28354129695143104No Hit
GGTATCAACGCAGAGTACTTTTTTT35940.2764643031045695No Hit
TATCAACGCAGAGTACTTTTTTTTT31190.2399254761778387No Hit
GTACATGGGATAAGCAGTGGTATCA29950.23038691925380794No Hit
TGATACCACTGCTTATGTACTCTGC29410.22623303156108485No Hit
CAGTGGTATCAACGCAGAGTACATA29020.22323300156078485No Hit
TACCACTGCTTATGTACTCTGCGTT27600.21230981540584637No Hit
GCTTATCCCATGTACTCTGCGTTGA27100.20846362309776945No Hit
ACCCTGTATCGCGCGCCTTTCCAGA26600.2046174307896925No Hit
GCAGAGTACATAAGCAGTGGTATCA24130.1856172407877925No Hit
GAACAAAAAAAAAAAAAAAAAAAAA22960.1766171507868925No Hit
CCCATATTCAGACAGGATACCACGT22210.17084786232477708No Hit
GTTAATGATAGTGTGTCGAAACACA21360.16430933540104634No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20660.15892466616973863No Hit
ATCATTAACTGAATCCATAGGTTAA20510.15777080847731553No Hit
GTATTTAGCCTTGGAGGATGGTCCC20450.15730926540034632No Hit
TCTAAGTACCCCGAGGAAAAGAAAT20440.15723234155418478No Hit
CCCCATGTACTCTGCGTTGATACCA20110.154693854630854No Hit
GGTTAATGAGGCGAACCGGGGGAAC19360.14892456616873861No Hit
GAGTACATGGGAAGCAGTGGTATCA19090.14684762232237705No Hit
GTTCACTATCGGTCAGTCAGGAGTA19060.14661685078389244No Hit
GCCTTGGAGGATGGTCCCCCCATAT18580.14292450616813862No Hit
ATCAACGCAGAGTACATAAGCAGTG18390.14146295309106938No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG18110.13930908539854628No Hit
GGATAAGCAGTGGTATCAACGCAGA18000.13846292309076935No Hit
GTACACAAGCAGTGGTATCAACGCA17580.13523212155198475No Hit
GAGTACATGATAAGCAGTGGTATCA17300.13307825385946168No Hit
CTATCGGTCAGTCAGGAGTATTTAG17200.13230901539784629No Hit
GCCCAGAGCCTGAATCAGTGTGTGT16780.12907821385906168No Hit
TATTCAGACAGGATACCACGTGTCC16570.12746281308966936No Hit
ATGTACTCTGCGTTGATACCACTGC16560.12738588924350783No Hit
GGTATCAACGCAGAGTACATGGGAA16090.1237704684739155No Hit
GAGTACATGGGATAAGCAGTGGTAT16060.1235396969354309No Hit
CATCTAAGTACCCCGAGGAAAAGAA16060.1235396969354309No Hit
CACACACACTGATTCAGGCTCTGGG15660.12046274308896934No Hit
ACTTAGATGTTTCAGTTCCCCCGGT15590.11992427616583858No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15520.11938580924270781No Hit
GTACATAAGCAGTTGTATCAACGCA15460.11892426616573859No Hit
GTCAGGAGTATTTAGCCTTGGAGGA15380.11830887539644629No Hit
GTATCAACGCAGAGTACATGGGAAG15320.11784733231947704No Hit
GGGATAAGCAGTGGTATCAACGCAG15310.1177704084733155No Hit
GCGTTGATACCACTGCTTCCCATGT14910.11469345462685396No Hit
GCTTATCATGTACTCTGCGTTGATA14350.1103857192418078No Hit
CCCCAGTAGCGGCGAGCGAACGGGG13810.10623183154908472No Hit
TACATAAGCAGTGGTATCAACGCAG13560.10430873539504626No Hit
CAGTGGTATCAACGCAGAGTACATG13420.10323180154878472No Hit
GGTATCAACGCAGAGTACATGGGAT13410.10315487770262317No Hit
GATAAGCAGTGGTATCAACGCAGAG13050.1003856192408078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAGT502.1593769E-719.0110111
CCTAGTT552.8343493E-819.0022322
CGCATTA307.7296916E-418.99711413
CTAGACT250.00603819418.99638417
AAGGAGT555.917209E-717.2701057
AGTTGTA2600.016.43981211
ATCACTC350.00216768316.2882545
TGTTCAG350.002172145516.28324110
CATAAGG601.4639772E-615.8358034
GCAGTTG2800.015.2655389
CTTGCAC508.7006076E-515.2023713
TCCTGCA508.703465E-515.2017852
CAGTTGT2900.015.06667610
CTAACGT456.753282E-414.7800834
TATTATA456.755253E-414.7795142
GCGGATT456.769064E-414.77553513
TAGTTCC851.7122147E-814.5316784
TCTTCGT1700.014.52720511
TACGCTG851.7191269E-814.5272057
GTTGTAT3100.014.40103712
Produced by FastQC (version 0.11.5)