##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295307_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1299987
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.49183337987226	31.0	30.0	33.0	25.0	34.0
2	29.32341246489388	31.0	30.0	34.0	22.0	34.0
3	29.805858058580586	31.0	30.0	34.0	25.0	34.0
4	32.98162289315201	35.0	33.0	37.0	25.0	37.0
5	33.611911503730425	35.0	33.0	37.0	28.0	37.0
6	33.63665328960982	36.0	35.0	37.0	27.0	37.0
7	33.75817296634505	36.0	35.0	37.0	28.0	37.0
8	33.626627804739584	36.0	35.0	37.0	27.0	37.0
9	34.429498141135255	38.0	34.0	39.0	24.0	39.0
10	34.348901181319505	38.0	34.0	39.0	24.0	39.0
11	34.830630613998444	38.0	34.0	39.0	27.0	39.0
12	34.8421907295996	38.0	35.0	39.0	27.0	39.0
13	34.53406764836879	38.0	34.0	39.0	25.0	39.0
14	34.620383126908195	38.0	33.0	40.0	19.0	41.0
15	35.16753705998598	38.0	33.0	40.0	25.0	41.0
16	35.0897162817782	38.0	33.0	40.0	25.0	41.0
17	35.195201182781055	38.0	34.0	40.0	25.0	41.0
18	35.11711578654248	38.0	33.0	40.0	25.0	41.0
19	35.2349261954158	39.0	34.0	40.0	24.0	41.0
20	35.256184869541	39.0	34.0	40.0	24.0	41.0
21	35.2732611941504	39.0	34.0	40.0	25.0	41.0
22	35.023664082794674	39.0	33.0	40.0	24.0	41.0
23	34.65263960331911	38.0	32.0	40.0	22.0	41.0
24	35.01270474243204	38.0	33.0	40.0	24.0	41.0
25	33.72180029492603	38.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4147063974113543
1101	2	0.6352452146667176
1101	3	0.3999410383951023
1101	4	0.2155395539073055
1101	5	0.3694247104702626
1101	6	0.2516077004399122
1101	7	0.17920563838929127
1101	8	0.202081663127899
1101	9	0.2233606492269402
1101	10	0.4931341128930882
1101	11	0.5149029870803474
1101	12	0.5745251173356039
1101	13	0.2683848219050944
1101	14	1.088322795048768
1101	15	0.6771410158598528
1101	16	0.5454247636973761
1101	17	0.8995375318088392
1101	18	0.9407246050895921
1101	19	0.8563425283198143
1101	20	0.8477053733853168
1101	21	0.7310900022958648
1101	22	0.6831079693949036
1101	23	-1.500806963247534
1101	24	0.046287695240799565
1101	25	0.5015856308627988
1102	1	0.7074822554947637
1102	2	0.49028382573663976
1102	3	0.6457944735995511
1102	4	0.5651907886277598
1102	5	0.28200946784735237
1102	6	0.35367328159305345
1102	7	0.3059656286635075
1102	8	0.3763929072446288
1102	9	0.5818262517488577
1102	10	0.9708312085877324
1102	11	0.6871265323689073
1102	12	0.6542640788433616
1102	13	0.6500759802771299
1102	14	1.172371457927973
1102	15	0.833577384107663
1102	16	0.5773648332856709
1102	17	0.8787798691328419
1102	18	0.8906470434459379
1102	19	1.0145845076670028
1102	20	1.0411034720641013
1102	21	1.1355878287225423
1102	22	1.0331069458907578
1102	23	1.034143456713693
1102	24	1.1214645742321565
1102	25	0.8477202551094649
1103	1	-0.20884229952080346
1103	2	-1.1365870205014872
1103	3	-0.21739782226142168
1103	4	0.23400863102322944
1103	5	-0.05800790427254299
1103	6	0.12854134893009217
1103	7	0.1457725625694195
1103	8	0.22369399394596456
1103	9	0.9889139915892642
1103	10	1.008208917132265
1103	11	0.7344053389409595
1103	12	0.5640311504606856
1103	13	0.4441139984729219
1103	14	-0.9405454603207062
1103	15	-0.05048059846651398
1103	16	0.14743517521652905
1103	17	0.6247770897000038
1103	18	0.7777351767778882
1103	19	0.7793975367037547
1103	20	0.726674482797641
1103	21	0.9995655124345717
1103	22	0.38870711487942344
1103	23	1.3441642772020543
1103	24	1.2020317018954358
1103	25	0.9652936672049179
1104	1	0.1477804813607051
1104	2	0.3268511192122787
1104	3	-0.1743654546315092
1104	4	0.009274634558394723
1104	5	0.01781777718507982
1104	6	-0.12450160629455098
1104	7	-0.15068176710838088
1104	8	0.1555915547743254
1104	9	-2.0329702984102127
1104	10	-0.3799309643358626
1104	11	-0.3668428680437259
1104	12	0.12565190214637312
1104	13	0.2897889045317754
1104	14	-1.6475740244662092
1104	15	-0.51988781614277
1104	16	-0.12435296555935338
1104	17	0.2707634745227381
1104	18	0.5436269105160889
1104	19	0.3951307360244485
1104	20	0.36093150085245895
1104	21	0.5575515998655192
1104	22	0.7954557841629821
1104	23	1.1496792736858978
1104	24	0.8277099172764011
1104	25	0.7751868074050137
1105	1	-0.16179644454049935
1105	2	-0.11670703457047793
1105	3	-1.5502878673002805
1105	4	-0.8289646244646178
1105	5	-0.5279077518219424
1105	6	-0.3021358459021002
1105	7	-0.104035880414024
1105	8	-0.17044440246890957
1105	9	0.23754389630558137
1105	10	0.5044738637197241
1105	11	-0.10558485772607185
1105	12	-0.23156990542626232
1105	13	-0.44150391443147896
1105	14	0.5599596470896699
1105	15	0.11877739943300725
1105	16	-0.2929381628978476
1105	17	0.2685495745186799
1105	18	0.10046824512515684
1105	19	0.1110481838580597
1105	20	-0.4710743144710179
1105	21	-0.03466582922666106
1105	22	0.18010282603090388
1105	23	0.5143592938959571
1105	24	0.2858891270017807
1105	25	0.4681849102461513
1106	1	-1.6327002667019244
1106	2	-0.9625865424401674
1106	3	-1.056121035522608
1106	4	-1.3117528200682322
1106	5	-1.14148467138439
1106	6	-0.7043552996877551
1106	7	-0.30777381260904235
1106	8	-0.6325199880249102
1106	9	-0.15554604983656617
1106	10	-1.8666992929403108
1106	11	-1.0552418339600393
1106	12	-0.8163478469579672
1106	13	-0.7024308702278717
1106	14	0.09788587096933554
1106	15	-0.6302511597947955
1106	16	-1.2863147236491983
1106	17	-0.42436386117442737
1106	18	-0.45935124557298934
1106	19	-0.21615840031643785
1106	20	0.16270947787090506
1106	21	-0.13427073894938957
1106	22	-0.07422421318047157
1106	23	-0.4300720321399183
1106	24	-0.7948895093532187
1106	25	-1.3282870520958738
1107	1	-0.018647823576490197
1107	2	0.07242776616934776
1107	3	0.018995841453328666
1107	4	0.24195431031107262
1107	5	-0.10286768708327543
1107	6	-0.08424768039207464
1107	7	-0.12258146298518824
1107	8	-0.2989454205001181
1107	9	0.30090099756156974
1107	10	-0.2056310694788266
1107	11	-0.13736270388503868
1107	12	-0.15529178418843514
1107	13	0.04295167261806654
1107	14	-1.3488464804022655
1107	15	-0.7712699373172001
1107	16	-0.3737339787757108
1107	17	-0.14856474109947015
1107	18	0.040325647669995135
1107	19	-0.08226635388974302
1107	20	0.13472998815051085
1107	21	0.26150350544035916
1107	22	-0.40644696425139415
1107	23	0.23172187354344942
1107	24	0.3182244394412166
1107	25	-0.01732260458580015
1108	1	-0.11440039111333533
1108	2	0.18005538818407274
1108	3	0.04411160413914672
1108	4	-0.19746314255938557
1108	5	-0.4091272740668117
1108	6	-0.35007928915418773
1108	7	-0.09979330982349666
1108	8	-0.38859781343816735
1108	9	0.36710903162226316
1108	10	0.45485447332752926
1108	11	0.24996962780414833
1108	12	-0.0024641463582781853
1108	13	-0.11567353484718268
1108	14	0.36603224846817994
1108	15	0.2631337983601725
1108	16	0.29118595109721923
1108	17	0.3231613517604899
1108	18	0.3065405111225914
1108	19	0.43699882151330627
1108	20	0.45999145950705156
1108	21	0.4014795306803549
1108	22	0.42761453801713856
1108	23	0.3401827263008599
1108	24	0.413588585313434
1108	25	-0.44331760654925034
1109	1	-0.9798144325810441
1109	2	-0.9142756886787389
1109	3	-0.7706116541427654
1109	4	-0.5887952071969593
1109	5	-0.9045297428445593
1109	6	-1.1187961612698416
1109	7	-0.8252893865026891
1109	8	-1.2165437410095166
1109	9	-0.7778539762601611
1109	10	-0.9275869122690352
1109	11	-0.8659185267143314
1109	12	-1.1515953000584744
1109	13	-1.0595954243689505
1109	14	-0.5474018648839163
1109	15	-1.2535008841159154
1109	16	-1.363042506420463
1109	17	-1.2927729428562813
1109	18	-1.9401275146367638
1109	19	-2.0643929145126023
1109	20	-1.854564423214235
1109	21	-1.6157698038193473
1109	22	-1.6795910387199697
1109	23	-1.1803008878436216
1109	24	-2.088141903781022
1109	25	-1.3383827817921699
1110	1	-0.14022311469548754
1110	2	0.01734528056060114
1110	3	-0.15109284591008532
1110	4	0.10691348777211829
1110	5	-0.011239147198949695
1110	6	0.09603641903591154
1110	7	0.12520884955598177
1110	8	0.08088842699577725
1110	9	0.04551955557410281
1110	10	0.14615131026847195
1110	11	-0.022062089161664744
1110	12	0.16311016220841168
1110	13	0.1493540188349911
1110	14	-2.6114860198822285
1110	15	-0.8502448531505351
1110	16	-0.1710542755512492
1110	17	-0.3783286200812057
1110	18	-2.72519804848492
1110	19	-0.5725482270468234
1110	20	-2.093626796750833
1110	21	-0.5038411803601193
1110	22	-2.206829292203814
1110	23	-0.9336119080730043
1110	24	-0.1703922739468524
1110	25	-0.11267946672511187
1111	1	-0.2757630939270541
1111	2	-1.4644044598340002
1111	3	-0.5598804886930573
1111	4	-0.26178675452625555
1111	5	-0.2365593271920261
1111	6	-0.09447067684502031
1111	7	-0.25137474338274757
1111	8	-0.20482462250231492
1111	9	-2.116625650379518
1111	10	-0.501520894646724
1111	11	-0.45405412792940325
1111	12	-0.4481177713326048
1111	13	-0.2931304048410226
1111	14	0.4817815467498292
1111	15	-0.06754755304668691
1111	16	0.061772827252490004
1111	17	-0.27037171457757125
1111	18	-0.05649347942988214
1111	19	-0.2520004596532601
1111	20	0.10761930916817164
1111	21	-0.8314978089513261
1111	22	-0.5034587487631725
1111	23	-0.2617401531405008
1111	24	-0.6184885010150367
1111	25	-2.466422111238785
1112	1	0.17402847799957755
1112	2	0.3232073334053531
1112	3	0.04022372534362617
1112	4	0.3551590794386783
1112	5	-0.18062986430485495
1112	6	0.0029208142367878054
1112	7	-0.04186284403456142
1112	8	-0.002655707338860225
1112	9	-0.14149238273260778
1112	10	0.4988492230835533
1112	11	0.06638571846542618
1112	12	0.07727824200679123
1112	13	-0.03782685777417072
1112	14	0.6710973973890049
1112	15	0.053437364339998794
1112	16	-0.13893772299762475
1112	17	-3.9252356678896803
1112	18	-1.3474647542517744
1112	19	-1.3984552876053584
1112	20	-0.2831492219823488
1112	21	-0.6090835802615189
1112	22	-1.9705050071634602
1112	23	-1.6883109428934233
1112	24	-0.444980340740166
1112	25	-0.24758084500665944
1113	1	-0.34827946275489907
1113	2	-0.08477688143592843
1113	3	-0.2454396133915253
1113	4	-0.5993985466462419
1113	5	-0.2776971863626727
1113	6	-0.4413130109824337
1113	7	-0.5623898568568819
1113	8	-0.5228865368757027
1113	9	-0.27571076007937734
1113	10	0.11676009125721265
1113	11	-0.45901441608641136
1113	12	-0.30364932747891515
1113	13	-0.9222158427379341
1113	14	-1.8787306047459325
1113	15	-2.100704275342757
1113	16	-0.7531751124905313
1113	17	-0.5371706856798184
1113	18	-0.8914803105232707
1113	19	-0.49929098788641113
1113	20	0.07325215512888406
1113	21	-1.9553560934653547
1113	22	-2.3702363870572043
1113	23	-0.9475589637543962
1113	24	-3.0542176728097843
1113	25	-0.9831677503204901
1114	1	-0.16537832753522252
1114	2	0.007719607696429875
1114	3	-0.16720773643550046
1114	4	0.10442512873510168
1114	5	-0.2777973850378572
1114	6	-0.10797021878978796
1114	7	-0.21313367545970152
1114	8	-0.28999017674914285
1114	9	0.28348395569298646
1114	10	-0.07329487710975258
1114	11	-0.18100086264998794
1114	12	-0.10436531746530875
1114	13	-0.15159624700827834
1114	14	0.4289906816798066
1114	15	-0.047470010840171994
1114	16	-0.18305932822454452
1114	17	-0.9741948398229567
1114	18	-0.5906169580321574
1114	19	-0.9318345782241266
1114	20	-2.152598594247614
1114	21	-1.2364284463024617
1114	22	-2.238923674819972
1114	23	-3.7619217393203215
1114	24	-2.84605688211699
1114	25	-2.0384398445197682
1115	1	0.1941946767703584
1115	2	0.28964778312357
1115	3	0.11108433707946119
1115	4	0.22572609970257673
1115	5	0.22074946088599035
1115	6	0.22425140208415684
1115	7	0.280580448039899
1115	8	0.2919491767862752
1115	9	0.4454668073490353
1115	10	0.8218818093772811
1115	11	0.24107264426837816
1115	12	0.3849038996328531
1115	13	0.0891655290081701
1115	14	0.8625490917185559
1115	15	0.3035260068582346
1115	16	0.3557703238387404
1115	17	0.33989291395629095
1115	18	0.6655599520170412
1115	19	-0.06449540225175099
1115	20	0.46102546908320363
1115	21	-0.5347261169133617
1115	22	0.547084711173774
1115	23	0.5342044430259847
1115	24	0.6265442878047338
1115	25	0.4346852489662112
1116	1	0.025574354632613705
1116	2	0.1960893673264934
1116	3	0.030603050401595056
1116	4	0.3140362907412353
1116	5	0.239057956758586
1116	6	0.09760751245539012
1116	7	-0.06217721251855579
1116	8	-0.091141494930298
1116	9	0.03908422616369478
1116	10	-0.027858343206396796
1116	11	0.07557550170022864
1116	12	-0.04960954087992775
1116	13	-0.7076880180097405
1116	14	0.47560870724365145
1116	15	0.2703639695870521
1116	16	0.2725238560590739
1116	17	0.5799883677269548
1116	18	0.39222051233966226
1116	19	0.6339563304701983
1116	20	0.29393618159615187
1116	21	-0.3890080970171752
1116	22	-0.10351018909239684
1116	23	-1.0448162848277462
1116	24	-0.5934885010150381
1116	25	-1.5677081194692377
1201	1	0.5751904715787504
1201	2	0.7182240642124356
1201	3	0.6396427285540405
1201	4	0.5596753792493132
1201	5	0.5972162394193603
1201	6	0.32798648180617107
1201	7	0.3566683743618597
1201	8	0.414675101101885
1201	9	0.6485139294988684
1201	10	0.9918317863925239
1201	11	0.5439420025294126
1201	12	0.5758664741068671
1201	13	0.5660113862037548
1201	14	1.3499683903098045
1201	15	0.9872824635370563
1201	16	0.761088747650497
1201	17	0.8270370748088354
1201	18	0.7458904343346262
1201	19	0.9774564226021312
1201	20	1.1061267718547398
1201	21	1.0147155686053324
1201	22	1.0830600461511395
1201	23	0.806895833592499
1201	24	1.2105883370170218
1201	25	1.2527666670917839
1202	1	0.4512925457610919
1202	2	0.7847892694834044
1202	3	0.4734409258562735
1202	4	0.4168935790127364
1202	5	0.5404762141544666
1202	6	0.22235537434499264
1202	7	0.14110660722580803
1202	8	0.21991643607266553
1202	9	0.8685420071821355
1202	10	0.11347947927590951
1202	11	0.25507939984370154
1202	12	0.3327245361439779
1202	13	0.3691634703062121
1202	14	1.093364735188139
1202	15	0.7280852642734104
1202	16	0.4371451139763636
1202	17	-1.7383922148860549
1202	18	0.1407056063292771
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2210	17	0.4296153051524385
2210	18	0.6112505496459235
2210	19	0.8947414925793424
2210	20	1.098429278015523
2210	21	1.0199905953408148
2210	22	1.2151333246186198
2210	23	1.5346123909924145
2210	24	1.172598380258755
2210	25	1.2611523089758236
2211	1	-0.03175231099222131
2211	2	-0.4729018813061039
2211	3	-0.08004574888996174
2211	4	0.2248166210011746
2211	5	0.1969615354427603
2211	6	0.2249972087621046
2211	7	0.15010204986619158
2211	8	0.08367748720232271
2211	9	0.5416432197564447
2211	10	0.684590216464386
2211	11	0.2942229466134947
2211	12	0.15611921882210567
2211	13	0.05362885441824261
2211	14	-1.0295874171507862
2211	15	-0.23597816720515397
2211	16	0.14696387272834244
2211	17	0.14653522400658403
2211	18	0.11821936632726704
2211	19	0.5079796153584581
2211	20	0.5871786153097531
2211	21	0.7589381966745492
2211	22	0.012510543313709377
2211	23	0.5913833817868266
2211	24	-0.26841817752699626
2211	25	0.31311961403078925
2212	1	-0.097262332892047
2212	2	-1.0732705176726611
2212	3	-0.4156833806260849
2212	4	0.030438004479329095
2212	5	0.03568826286377913
2212	6	-0.08352445665222064
2212	7	-0.042622840795232264
2212	8	0.10081972047079546
2212	9	-2.246299673717921
2212	10	-0.10587147810689856
2212	11	0.07599407095441535
2212	12	-0.08101350953656095
2212	13	0.01994037141469107
2212	14	0.71529245491827
2212	15	0.15206812427446437
2212	16	0.2799805898095613
2212	17	0.27439616974510983
2212	18	0.014758423157637424
2212	19	-0.6392340974005961
2212	20	0.3984868890768496
2212	21	0.38960822859306177
2212	22	-1.6971485001584057
2212	23	-0.33196465846774714
2212	24	0.22539785109049149
2212	25	-0.5525015125579102
2213	1	0.27798478086042167
2213	2	0.6186163422462094
2213	3	0.15753750310612347
2213	4	0.3505186426801359
2213	5	0.5135423496423215
2213	6	0.3262655634456735
2213	7	0.12456785087667299
2213	8	0.2204137346667352
2213	9	0.6657455034025332
2213	10	0.716410065115916
2213	11	0.1421946636164222
2213	12	0.13159144958948588
2213	13	-0.10377021882241877
2213	14	-1.6223872034585298
2213	15	-0.3750570539967839
2213	16	0.04488488845861127
2213	17	-2.252241151521261
2213	18	-0.9116302431062522
2213	19	-0.64222823243054
2213	20	0.22250079731698946
2213	21	-0.05403339584335498
2213	22	0.4288365641014451
2213	23	0.8654863195067648
2213	24	0.6170783890072968
2213	25	-0.9572091066049921
2214	1	0.22509630835803307
2214	2	-0.012569654665114172
2214	3	0.044924804925663864
2214	4	0.3435289001495292
2214	5	0.3592779559824777
2214	6	0.15797864045611476
2214	7	0.1365337533492763
2214	8	0.13589075610035195
2214	9	0.48460067136207385
2214	10	0.516884117909612
2214	11	-0.16712144985392996
2214	12	-0.3299736561801794
2214	13	-0.19365614949002463
2214	14	0.7888920924099878
2214	15	0.4318718203006142
2214	16	0.5416953687881829
2214	17	0.24502705307198624
2214	18	0.2831531553054347
2214	19	-0.9067346561308369
2214	20	0.08311698915619559
2214	21	0.46182488059947246
2214	22	0.5660802839830836
2214	23	1.1023813606049444
2214	24	0.9461491946060434
2214	25	0.9562177547160786
2215	1	-0.41227030497722694
2215	2	0.17968958851050587
2215	3	0.14408304979973252
2215	4	0.14789061022423766
2215	5	0.2524446676620613
2215	6	0.10253148985550098
2215	7	-0.015471838649865788
2215	8	-0.011861320992395008
2215	9	0.25614078607522117
2215	10	0.6393264883853007
2215	11	0.23861125225882773
2215	12	0.16648968347137583
2215	13	-0.04536227565149886
2215	14	0.9194550520650111
2215	15	0.30181310102602765
2215	16	0.328294765095265
2215	17	0.37397878308645716
2215	18	0.3028478774663199
2215	19	-0.2821827671893189
2215	20	-1.509961434280946
2215	21	-1.8899343991063802
2215	22	-0.2685875658801109
2215	23	0.5970019756427618
2215	24	0.7443559705131619
2215	25	-0.22785399559114694
2216	1	0.21967795781170452
2216	2	-0.21188113498243055
2216	3	0.015617814954101306
2216	4	0.38334329919158705
2216	5	0.3120694542611844
2216	6	-0.04603273508647021
2216	7	0.05519862387763652
2216	8	0.1107747555016445
2216	9	0.2901986357386619
2216	10	-2.0399786271884253
2216	11	-0.5722404440164794
2216	12	-0.09378146938179555
2216	13	0.12617621093506415
2216	14	-0.727647347793571
2216	15	-0.4956539991874891
2216	16	0.11053236923721954
2216	17	0.5221113218940161
2216	18	0.23573510411549847
2216	19	-1.4767990177533434
2216	20	-1.0666809452847517
2216	21	-0.7019529969637688
2216	22	-0.5451006769509021
2216	23	-4.099324970782572
2216	24	-1.966438741331693
2216	25	-1.2145949978658734
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	10.0
5	15.0
6	17.0
7	67.0
8	61.0
9	180.0
10	476.0
11	1130.0
12	2263.0
13	4125.0
14	6236.0
15	9098.0
16	12324.0
17	14647.0
18	14798.0
19	14227.0
20	13476.0
21	13154.0
22	12204.0
23	12350.0
24	12865.0
25	13054.0
26	14490.0
27	16236.0
28	18898.0
29	22572.0
30	27938.0
31	34706.0
32	46066.0
33	61316.0
34	82588.0
35	112549.0
36	157451.0
37	225189.0
38	323204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.56585807508599	18.098536897829213	12.138574345361095	20.197030681723696
2	12.32876712328767	24.949894788673337	37.65949998653603	25.061838101502964
3	21.178862226517474	31.886788968518637	30.35673339868547	16.57761540627842
4	12.520045431180687	21.2137604767196	31.254174528374477	35.01201956372523
5	9.2656168112315	46.83469586745441	31.42809429151689	12.471593029797209
6	28.191625224648565	31.697373765971587	24.61009626037076	15.500904749009084
7	21.58948926584273	40.82703481215225	18.52850029543552	19.0549756265695
8	20.84080701098294	37.563454120276	25.275535353861805	16.320203514879257
9	27.73803340374205	23.96304888981228	16.312276125289042	31.986641581156626
10	14.796737208654614	29.037442700342993	27.063056935739755	29.102763155262647
11	27.704249799189373	30.72456475642671	18.708703024931296	22.862482419452625
12	26.12748390352811	28.53489601244481	22.015142108247378	23.322477975779705
13	28.251986387847854	22.23461804494965	31.509685540204398	18.003710026998103
14	29.570824169651118	26.869814407540048	22.026388326712908	21.532973096095926
15	32.4642646081111	23.721636379306098	23.45623722636724	20.357861786215558
16	24.637690079986704	25.556352310812514	28.80467732950891	21.001280279691873
17	19.591360025112987	34.810798583701484	25.32372207680696	20.27411931437857
18	19.91042348409713	24.531208282711546	37.592052142426816	17.966316090764508
19	22.39198220661651	35.0124518294611	21.117948867872123	21.477617096050267
20	20.205918918752577	30.982003828187025	28.929584746336452	19.882492506723946
21	22.523709734992554	33.46088278374752	22.473782356633805	21.541625124626123
22	19.91837080121251	31.24998076597578	20.246272446106264	28.58537598670544
23	25.25688725580708	25.21211086081465	22.364886074079546	27.16611580929872
24	18.97064005330059	30.417110966473253	19.76453264276449	30.847716337461666
25	24.266190473990363	32.73401127646435	21.851884364647773	21.147913884897516
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8563.0
1	8563.0
2	7313.0
3	6063.0
4	6063.0
5	6063.0
6	8436.0
7	10809.0
8	10809.0
9	10809.0
10	9872.0
11	8935.0
12	8935.0
13	8935.0
14	9279.5
15	9624.0
16	9624.0
17	9624.0
18	11618.0
19	13612.0
20	13612.0
21	13612.0
22	16476.5
23	19341.0
24	19341.0
25	19341.0
26	25390.5
27	31440.0
28	31440.0
29	31440.0
30	35776.0
31	40112.0
32	40112.0
33	40112.0
34	46765.0
35	53418.0
36	53418.0
37	53418.0
38	78303.0
39	103188.0
40	103188.0
41	103188.0
42	251880.0
43	400572.0
44	400572.0
45	400572.0
46	314269.0
47	227966.0
48	227966.0
49	227966.0
50	183285.5
51	138605.0
52	138605.0
53	138605.0
54	110772.5
55	82940.0
56	82940.0
57	82940.0
58	69199.5
59	55459.0
60	55459.0
61	55459.0
62	50473.5
63	45488.0
64	45488.0
65	45488.0
66	32866.0
67	20244.0
68	20244.0
69	20244.0
70	17262.5
71	14281.0
72	14281.0
73	14281.0
74	10427.5
75	6574.0
76	6574.0
77	6574.0
78	4316.0
79	2058.0
80	2058.0
81	2058.0
82	1283.0
83	508.0
84	508.0
85	508.0
86	324.5
87	141.0
88	141.0
89	141.0
90	88.5
91	36.0
92	36.0
93	36.0
94	21.5
95	7.0
96	7.0
97	7.0
98	5.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.084847002316177
2	0.017077093847861557
3	0.027769508464315414
4	0.03376956846491542
5	0.043846592312076965
6	0.013769368462915398
7	0.01623093154008463
8	0.03276955846481542
9	0.03323110154178465
10	0.018538646924930787
11	0.019615580771192326
12	0.012923206155138475
13	0.02046174307896925
14	0.03638497923440773
15	0.03346187308026926
16	0.020846362309776944
17	0.02007712384816156
18	0.02430793538704618
19	0.01369244461675386
20	0.011923196155038474
21	0.007615460769992316
22	0.01592323615543848
23	0.015000150001500014
24	0.014538606924530784
25	0.10407796385656164
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1299987.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.55522700773969
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.10449257926243	15.273510827117963
2	7.809144999207131	3.0541920639170117
3	3.4268679207123522	2.0103954034521276
4	2.077817253062698	1.6252875225695664
5	1.3608492346509304	1.3305857853453877
6	1.0205320634574966	1.1974041701753009
7	0.7412342708903331	1.0146503103223812
8	0.6107696563839662	0.9554991424022098
9	0.4903145943357924	0.8629391877699794
>10	3.668661339633055	14.009615883315357
>50	0.35685484741009005	4.8114643693679415
>100	0.25023118257043386	10.445407843694
>500	0.03895108030577508	5.400433272614764
>1k	0.03541007300525008	13.902820759473652
>5k	0.0035410073005250073	4.904483750948527
>10k+	0.004327897811752787	19.201309707513833
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	68646	5.280514343604974	No Hit
GCTTATGTACTCTGCGTTGATACCA	41250	3.1731086541634648	No Hit
CTTATGTACTCTGCGTTGATACCAC	28564	2.1972527417581866	No Hit
GAGTACATAAGCAGTGGTATCAACG	20401	1.5693233855415478	No Hit
TATGTACTCTGCGTTGATACCACTG	18855	1.4503991193758092	No Hit
GCGTTGATACCACTGCTTATGTACT	14709	1.1314728531900704	No Hit
CATAAGCAGTGGTATCAACGCAGAG	12487	0.9605480670191316	No Hit
CCCATGTACTCTGCGTTGATACCAC	11685	0.8988551423975779	No Hit
CTGCTTATGTACTCTGCGTTGATAC	11239	0.8645471070095316	No Hit
ACGCAGAGTACATAAGCAGTGGTAT	11086	0.8527777585468163	No Hit
ACTCTGCGTTGATACCACTGCTTAT	10643	0.8187004946972547	No Hit
GTATCAACGCAGAGTACATAAGCAG	9364	0.7203148954566468	No Hit
CATGTACTCTGCGTTGATACCACTG	8196	0.6304678431399698	No Hit
TATCAACGCAGAGTACATAAGCAGT	8058	0.6198523523696775	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	7843	0.6033137254449468	No Hit
GGTATCAACGCAGAGTACATAAGCA	7807	0.6005444669831315	No Hit
GTGGTATCAACGCAGAGTACATAAG	6292	0.4840048400484005	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	5889	0.45300453004530045	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5160	0.39692704619353886	No Hit
GTACTGGTTCACTATCGGTCAGTCA	5136	0.39508087388566193	No Hit
ACATAAGCAGTGGTATCAACGCAGA	4991	0.38392691619223884	No Hit
GTGGTATCAACGCAGAGTACATGGG	4751	0.3654651931134696	No Hit
ATCATGTACTCTGCGTTGATACCAC	4503	0.34638807926540804	No Hit
GTACATGATAAGCAGTGGTATCAAC	4479	0.3445419069575311	No Hit
CCACTGCTTATGTACTCTGCGTTGA	4400	0.3384649231107696	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4272	0.32861867080209267	No Hit
GATACCACTGCTTATGTACTCTGCG	4240	0.3261571077249234	No Hit
GTTGATACCACTGCTTATGTACTCT	4058	0.31215696772352336	No Hit
GGATACCACGTGTCCCGCCCTACTC	3926	0.3020030200302003	No Hit
ATACAGGGTGACAGCCCCGTACACA	3836	0.29507987387566187	No Hit
GCAGTGGTATCAACGCAGAGTACAT	3818	0.2936952446447541	No Hit
GTACTCTGCGTTGATACCACTGCTT	3814	0.293387549260108	No Hit
ATACCACTGCTTATGTACTCTGCGT	3686	0.28354129695143104	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3594	0.2764643031045695	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3119	0.2399254761778387	No Hit
GTACATGGGATAAGCAGTGGTATCA	2995	0.23038691925380794	No Hit
TGATACCACTGCTTATGTACTCTGC	2941	0.22623303156108485	No Hit
CAGTGGTATCAACGCAGAGTACATA	2902	0.22323300156078485	No Hit
TACCACTGCTTATGTACTCTGCGTT	2760	0.21230981540584637	No Hit
GCTTATCCCATGTACTCTGCGTTGA	2710	0.20846362309776945	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	2660	0.2046174307896925	No Hit
GCAGAGTACATAAGCAGTGGTATCA	2413	0.1856172407877925	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	2296	0.1766171507868925	No Hit
CCCATATTCAGACAGGATACCACGT	2221	0.17084786232477708	No Hit
GTTAATGATAGTGTGTCGAAACACA	2136	0.16430933540104634	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2066	0.15892466616973863	No Hit
ATCATTAACTGAATCCATAGGTTAA	2051	0.15777080847731553	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	2045	0.15730926540034632	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	2044	0.15723234155418478	No Hit
CCCCATGTACTCTGCGTTGATACCA	2011	0.154693854630854	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	1936	0.14892456616873861	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1909	0.14684762232237705	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	1906	0.14661685078389244	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	1858	0.14292450616813862	No Hit
ATCAACGCAGAGTACATAAGCAGTG	1839	0.14146295309106938	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	1811	0.13930908539854628	No Hit
GGATAAGCAGTGGTATCAACGCAGA	1800	0.13846292309076935	No Hit
GTACACAAGCAGTGGTATCAACGCA	1758	0.13523212155198475	No Hit
GAGTACATGATAAGCAGTGGTATCA	1730	0.13307825385946168	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	1720	0.13230901539784629	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	1678	0.12907821385906168	No Hit
TATTCAGACAGGATACCACGTGTCC	1657	0.12746281308966936	No Hit
ATGTACTCTGCGTTGATACCACTGC	1656	0.12738588924350783	No Hit
GGTATCAACGCAGAGTACATGGGAA	1609	0.1237704684739155	No Hit
GAGTACATGGGATAAGCAGTGGTAT	1606	0.1235396969354309	No Hit
CATCTAAGTACCCCGAGGAAAAGAA	1606	0.1235396969354309	No Hit
CACACACACTGATTCAGGCTCTGGG	1566	0.12046274308896934	No Hit
ACTTAGATGTTTCAGTTCCCCCGGT	1559	0.11992427616583858	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1552	0.11938580924270781	No Hit
GTACATAAGCAGTTGTATCAACGCA	1546	0.11892426616573859	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	1538	0.11830887539644629	No Hit
GTATCAACGCAGAGTACATGGGAAG	1532	0.11784733231947704	No Hit
GGGATAAGCAGTGGTATCAACGCAG	1531	0.1177704084733155	No Hit
GCGTTGATACCACTGCTTCCCATGT	1491	0.11469345462685396	No Hit
GCTTATCATGTACTCTGCGTTGATA	1435	0.1103857192418078	No Hit
CCCCAGTAGCGGCGAGCGAACGGGG	1381	0.10623183154908472	No Hit
TACATAAGCAGTGGTATCAACGCAG	1356	0.10430873539504626	No Hit
CAGTGGTATCAACGCAGAGTACATG	1342	0.10323180154878472	No Hit
GGTATCAACGCAGAGTACATGGGAT	1341	0.10315487770262317	No Hit
GATAAGCAGTGGTATCAACGCAGAG	1305	0.1003856192408078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.8461923080769267E-4	0.0
2	0.0	0.0	0.0	3.8461923080769267E-4	0.0
3	0.0	0.0	0.0	3.8461923080769267E-4	0.0
4	0.0	0.0	0.0	3.8461923080769267E-4	0.0
5	1.5384769232307707E-4	0.0	0.0	3.8461923080769267E-4	0.0
6	1.5384769232307707E-4	0.0	0.0	3.8461923080769267E-4	0.0
7	1.5384769232307707E-4	0.0	0.0	3.8461923080769267E-4	0.0
8	2.3077153848461562E-4	0.0	0.0	3.8461923080769267E-4	0.0
9	2.3077153848461562E-4	0.0	0.0	3.8461923080769267E-4	0.0
10	2.3077153848461562E-4	0.0	0.0	4.6154307696923125E-4	0.0
11	2.3077153848461562E-4	0.0	0.0	4.6154307696923125E-4	0.0
12	2.3077153848461562E-4	0.0	0.0	4.6154307696923125E-4	0.0
13	2.3077153848461562E-4	0.0	0.0	4.6154307696923125E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTAGT	50	2.1593769E-7	19.011011	1
CCTAGTT	55	2.8343493E-8	19.002232	2
CGCATTA	30	7.7296916E-4	18.997114	13
CTAGACT	25	0.006038194	18.996384	17
AAGGAGT	55	5.917209E-7	17.270105	7
AGTTGTA	260	0.0	16.439812	11
ATCACTC	35	0.002167683	16.288254	5
TGTTCAG	35	0.0021721455	16.283241	10
CATAAGG	60	1.4639772E-6	15.835803	4
GCAGTTG	280	0.0	15.265538	9
CTTGCAC	50	8.7006076E-5	15.202371	3
TCCTGCA	50	8.703465E-5	15.201785	2
CAGTTGT	290	0.0	15.066676	10
CTAACGT	45	6.753282E-4	14.780083	4
TATTATA	45	6.755253E-4	14.779514	2
GCGGATT	45	6.769064E-4	14.775535	13
TAGTTCC	85	1.7122147E-8	14.531678	4
TCTTCGT	170	0.0	14.527205	11
TACGCTG	85	1.7191269E-8	14.527205	7
GTTGTAT	310	0.0	14.401037	12
>>END_MODULE