FastQCFastQC Report
Wed 25 May 2016
SRR1295307_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295307_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299987
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA749365.764365335961052No Hit
GCTTATGTACTCTGCGTTGATACCA460703.5438815926620806No Hit
CTTATGTACTCTGCGTTGATACCAC335502.580795038719618No Hit
GAGTACATAAGCAGTGGTATCAACG225811.7370173701737017No Hit
TATGTACTCTGCGTTGATACCACTG184351.418091103987963No Hit
GCGTTGATACCACTGCTTATGTACT164951.2688588424345781No Hit
GTATCAACGCAGAGTACTTTTTTTT158201.2169352462755396No Hit
CATAAGCAGTGGTATCAACGCAGAG156741.205704364735955No Hit
CCCATGTACTCTGCGTTGATACCAC140301.0792415616463857No Hit
ACGCAGAGTACATAAGCAGTGGTAT132741.0210871339482626No Hit
CTGCTTATGTACTCTGCGTTGATAC132221.0170870939478625No Hit
ACTCTGCGTTGATACCACTGCTTAT125750.9673173654813471No Hit
GGTATCAACGCAGAGTACTTTTTTT107470.8267005746980546No Hit
GTATCAACGCAGAGTACATAAGCAG103770.7982387516182854No Hit
TATCAACGCAGAGTACTTTTTTTTT100310.7716231008463931No Hit
CATGTACTCTGCGTTGATACCACTG95060.7312380816115853No Hit
TATCAACGCAGAGTACATAAGCAGT92430.7110071100711007No Hit
GTACTTTTTTTTTTTTTTTTTTTTT91610.7046993546858545No Hit
GGTATCAACGCAGAGTACATAAGCA89230.6863914792994084No Hit
GTGGTATCAACGCAGAGTACATAAG69200.5323130154378467No Hit
AAGCAGTGGTATCAACGCAGAGTAC68070.5236206208215928No Hit
ACATAAGCAGTGGTATCAACGCAGA63810.49085106235677745No Hit
ACGCAGAGTACTTTTTTTTTTTTTT58650.4511583577374236No Hit
GTACTGGTTCACTATCGGTCAGTCA56570.43515819773582354No Hit
GTGGTATCAACGCAGAGTACATGGG53940.41492722619533884No Hit
ATCATGTACTCTGCGTTGATACCAC51410.3954654931164696No Hit
GATACCACTGCTTATGTACTCTGCG50880.3913885292699081No Hit
CCACTGCTTATGTACTCTGCGTTGA50510.3885423469619312No Hit
GTACATGATAAGCAGTGGTATCAAC46720.35938820926670806No Hit
ATACAGGGTGACAGCCCCGTACACA45700.35154197695823114No Hit
GGATACCACGTGTCCCGCCCTACTC45270.34823425157328497No Hit
GCAGTGGTATCAACGCAGAGTACAT44800.34461883080369266No Hit
GTACATGGGAAGCAGTGGTATCAAC42450.3265417269557311No Hit
ATACCACTGCTTATGTACTCTGCGT41790.3214647531090695No Hit
GTACTCTGCGTTGATACCACTGCTT40190.3091569377232234No Hit
GTTGATACCACTGCTTATGTACTCT39680.30523382156898493No Hit
CAGTGGTATCAACGCAGAGTACATA36280.27907971387406183No Hit
TGATACCACTGCTTATGTACTCTGC34300.2638487923340772No Hit
TACCACTGCTTATGTACTCTGCGTT32530.25023327156348485No Hit
ACCCTGTATCGCGCGCCTTTCCAGA31090.23915623771622332No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30560.23507927386966176No Hit
GTACATGGGATAAGCAGTGGTATCA29840.229540756946031No Hit
GCTTATCCCATGTACTCTGCGTTGA29780.22907921386906177No Hit
GCAGAGTACATAAGCAGTGGTATCA28150.21654062694473097No Hit
CCCATATTCAGACAGGATACCACGT25580.1967711984812156No Hit
TCTAAGTACCCCGAGGAAAAGAAAT25100.19307885386546172No Hit
ATCATTAACTGAATCCATAGGTTAA24860.1912326815575848No Hit
CCCCATGTACTCTGCGTTGATACCA24020.18477107848001556No Hit
ATCAACGCAGAGTACATAAGCAGTG22780.1752325215559848No Hit
GTTAATGATAGTGTGTCGAAACACA22640.17415558770972325No Hit
GTATTTAGCCTTGGAGGATGGTCCC22570.1736171207865925No Hit
GCAGAGTACTTTTTTTTTTTTTTTT22360.17200172001720018No Hit
GCCTTGGAGGATGGTCCCCCCATAT21950.16884784232457709No Hit
GGATAAGCAGTGGTATCAACGCAGA21070.1620785438623617No Hit
GTTCACTATCGGTCAGTCAGGAGTA20750.15961698078519246No Hit
CTATCGGTCAGTCAGGAGTATTTAG20630.158693894631254No Hit
GGTTAATGAGGCGAACCGGGGGAAC20620.15861697078509246No Hit
GCCCAGAGCCTGAATCAGTGTGTGT20220.15554001693863093No Hit
GAGTACATGGGAAGCAGTGGTATCA20180.15523232155398475No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG19930.15330922539994632No Hit
GAGTACATGATAAGCAGTGGTATCA19900.1530784538614617No Hit
TATTCAGACAGGATACCACGTGTCC19630.15100151001510015No Hit
GTACACAAGCAGTGGTATCAACGCA19580.15061689078429247No Hit
CACACACACTGATTCAGGCTCTGGG19160.14738608924550783No Hit
GGTATCAACGCAGAGTACATGGGAA18950.14577068847611552No Hit
GTCAGGAGTATTTAGCCTTGGAGGA18740.14415528770672323No Hit
CATCTAAGTACCCCGAGGAAAAGAA18160.139693704629354No Hit
ACTTAGATGTTTCAGTTCCCCCGGT18040.13877061847541552No Hit
TACATAAGCAGTGGTATCAACGCAG17810.13700137001370014No Hit
GGGATAAGCAGTGGTATCAACGCAG17790.13684752232137706No Hit
ATGTACTCTGCGTTGATACCACTGC17550.13500135001350014No Hit
GAGTACATGGGATAAGCAGTGGTAT17090.13146285309006936No Hit
CCCCAGTAGCGGCGAGCGAACGGGG16660.12815512770512322No Hit
CAGTGGTATCAACGCAGAGTACATG16570.12746281308966936No Hit
GTATCAACGCAGAGTACATGGGAAG16480.1267704984742155No Hit
ATCAACGCAGAGTACTTTTTTTTTT16340.12569356462795397No Hit
GCGTTGATACCACTGCTTCCCATGT16280.12523202155098476No Hit
AAAAAGTACTCTGCGTTGATACCAC16190.12453970693553089No Hit
GCTTATCATGTACTCTGCGTTGATA16170.12438585924320783No Hit
GTGGTATCAACGCAGAGTACTTTTT15980.1229243061661386No Hit
TCACGGTACTGGTTCACTATCGGTC15360.1181550277041232No Hit
GCCTGAATCAGTGTGTGTGTTAGTG15180.11677039847321549No Hit
GATAAGCAGTGGTATCAACGCAGAG15110.11623193155008472No Hit
CGGTACTGGTTCACTATCGGTCAGT15060.11584731231927703No Hit
ACTCTGCGTTGATACCACTGCTTCC14950.11500115001150012No Hit
GGTATCAACGCAGAGTACATGGGAT14880.11446268308836934No Hit
GGTATCAACGCAGAGTACATGGGGG14820.11400114001140012No Hit
ACGCAGAGTACATGGGAAGCAGTGG14720.11323190154978473No Hit
ACGCAGAGTACATGATAAGCAGTGG14360.11046264308796935No Hit
CTGCGTTGATACCACTGCTTATGTA14270.10977032847251549No Hit
GTATCAACGCAGAGTACATGGGATA14120.10861647078009241No Hit
TATCAACGCAGAGTACATGGGAAGC14100.10846262308776934No Hit
GCGTTGATACCACTGCTTATCATGT14020.10784723231847702No Hit
AGCTCACAGCATGTGCATTTTTGTG13510.10392411616423856No Hit
ACGCAGAGTACATGGGATAAGCAGT13360.10277025847181549No Hit
TCATTAACCTATGGATTCAGTTAAT13010.10007792385616164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAC250.00600388319.0151332
AGGACTG250.00600388319.0151335
GGGTCAC250.006044057718.993196
GATTGTG453.55023E-516.8821897
CTTATAC1500.016.497571
TGAGCGC350.002158431316.2986875
TATGTCC350.002158431316.2986874
ACAACCC601.4724392E-615.82705111
GATCGAT807.750714E-915.43137511
ACCGTTG508.7417335E-515.19396911
TATACAC1650.014.9816223
CCCCTAA456.6658226E-414.8055131
CCTAGTT456.720577E-414.7895492
TATTGAG456.720577E-414.7895492
GGTCCGA653.3688302E-614.61633319
CCCCTGT400.00521322714.2767441
GGTAAAC400.00521322714.2767441
AAGACTT400.00525067814.261355
GAGCGGC400.00529240514.24434617
ACTCGAG400.00529240514.24434613
Produced by FastQC (version 0.11.5)