##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295307_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1299987 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.264309566172585 31.0 31.0 34.0 28.0 34.0 2 31.3083076984616 31.0 31.0 34.0 28.0 34.0 3 31.51781363967486 31.0 31.0 34.0 28.0 34.0 4 34.954558776357 37.0 35.0 37.0 32.0 37.0 5 35.224391474683976 37.0 35.0 37.0 32.0 37.0 6 35.04954972626649 37.0 35.0 37.0 32.0 37.0 7 34.93279855875482 37.0 35.0 37.0 32.0 37.0 8 34.95913959139591 37.0 35.0 37.0 32.0 37.0 9 36.34310727722662 39.0 37.0 39.0 32.0 39.0 10 36.4109379555334 39.0 37.0 39.0 32.0 39.0 11 36.48311790810216 39.0 37.0 39.0 32.0 39.0 12 36.43485896397426 39.0 37.0 39.0 32.0 39.0 13 36.11273805045743 39.0 37.0 39.0 31.0 39.0 14 37.225820719745656 40.0 37.0 41.0 31.0 41.0 15 37.26246724005701 40.0 37.0 41.0 31.0 41.0 16 37.05058896742813 39.0 36.0 41.0 31.0 41.0 17 37.366901361321304 40.0 37.0 41.0 32.0 41.0 18 37.3687152256138 40.0 37.0 41.0 31.0 41.0 19 37.535669972084335 40.0 37.0 41.0 31.0 41.0 20 37.55516478241705 40.0 37.0 41.0 32.0 41.0 21 37.5255583325064 40.0 37.0 41.0 31.0 41.0 22 37.526648343406514 40.0 37.0 41.0 31.0 41.0 23 37.54046309693866 40.0 37.0 41.0 31.0 41.0 24 37.56017252480217 40.0 37.0 41.0 31.0 41.0 25 36.28741825879797 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09382214117413312 1101 2 0.18917342111304336 1101 3 0.02016698119089 1101 4 -0.08215146561865083 1101 5 0.012601849648568475 1101 6 -0.18575143390523863 1101 7 -0.14571268441795127 1101 8 -0.07412184702066327 1101 9 0.10720941612402157 1101 10 0.019704692766737253 1101 11 -0.021577588716525042 1101 12 0.09713248923733175 1101 13 0.297654243783974 1101 14 0.28518278366953353 1101 15 0.20869760610619892 1101 16 0.24543804502644662 1101 17 0.22291833587959076 1101 18 0.22142814470527838 1101 19 0.27773345027707563 1101 20 0.2643764643898834 1101 21 0.31212442988125133 1101 22 0.26376458919101964 1101 23 0.16066049994450537 1101 24 0.18348560397020464 1101 25 0.14136766701683712 1102 1 -0.054581217317430486 1102 2 0.18347899351897468 1102 3 0.07708379726420134 1102 4 0.031290488964728524 1102 5 -0.1616567214497664 1102 6 -0.04719618240530821 1102 7 0.0735388794214984 1102 8 0.15011567983383856 1102 9 0.3215424184411333 1102 10 0.3623336904865653 1102 11 0.34484014177036215 1102 12 0.34480052240684955 1102 13 0.5156508916830731 1102 14 0.6429626384118237 1102 15 0.5863793742021244 1102 16 0.5991418569053835 1102 17 0.4823711455981865 1102 18 0.5357260483191411 1102 19 0.44109969051696396 1102 20 0.5477662901595224 1102 21 0.40041289628759813 1102 22 0.3066516597374189 1102 23 0.48793868288014863 1102 24 0.49592697091242144 1102 25 0.3794152604590195 1103 1 -0.2084398671788179 1103 2 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2214 25 0.0384214740229325 2215 1 -0.03891415266660303 2215 2 -0.14821667635822067 2215 3 -0.0264164412505572 2215 4 -0.55499739420992 2215 5 0.003652157457345595 2215 6 0.021176669008731608 2215 7 0.0531557484350742 2215 8 -0.05043754572207604 2215 9 -0.02108402884710614 2215 10 -0.12883459982678858 2215 11 -0.060042792981370496 2215 12 -0.13585542534232786 2215 13 0.008904922306712137 2215 14 0.13350821678942992 2215 15 -0.011601706384610111 2215 16 -0.08196483318076275 2215 17 -0.06629528577068555 2215 18 0.007349456037829327 2215 19 -0.027454275859348343 2215 20 0.021300573323870253 2215 21 0.06924687623030223 2215 22 -0.018385588087227234 2215 23 -0.023533233500899087 2215 24 -0.14758538176220526 2215 25 -0.1405873498758865 2216 1 0.04206560739405418 2216 2 -0.06230718158915849 2216 3 -0.0017735824463471772 2216 4 0.22441680584497448 2216 5 0.18389111992205187 2216 6 -0.33368561474117087 2216 7 -0.2625477650620951 2216 8 -0.17125337669462226 2216 9 -0.05555681820008829 2216 10 -0.15151230984348985 2216 11 -0.018454160096005978 2216 12 -0.15746345619300683 2216 13 -0.40880537350062696 2216 14 -0.4172379303653031 2216 15 -0.166381420857725 2216 16 -0.35773086673877685 2216 17 -0.5081039483344725 2216 18 -0.4357161202492037 2216 19 -0.28915027206348753 2216 20 -0.27377201786284644 2216 21 -0.20981588107184734 2216 22 -0.184157715490457 2216 23 -0.35016170896968646 2216 24 -0.3665632904103475 2216 25 -0.6873680784392704 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 6.0 8 32.0 9 88.0 10 136.0 11 263.0 12 404.0 13 689.0 14 1203.0 15 2137.0 16 3335.0 17 4595.0 18 5429.0 19 5798.0 20 5957.0 21 6466.0 22 6277.0 23 6176.0 24 6763.0 25 7178.0 26 7777.0 27 9018.0 28 10445.0 29 13379.0 30 17320.0 31 23501.0 32 32273.0 33 45150.0 34 64906.0 35 95239.0 36 146221.0 37 253321.0 38 518505.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.619413475054394 17.539676121156106 12.235688290682226 21.605222113107274 2 11.949965653502689 23.060845993075315 38.410845646918006 26.57834270650399 3 21.166693974758093 30.029823512805187 31.892927665865884 16.910554846570836 4 12.204071459780796 19.814519652670512 32.18797885499425 35.79343003255445 5 9.213771606207402 44.320725557370274 33.452919701565406 13.012583134856923 6 29.927113422284446 29.187939675298953 24.83274549699415 16.052201405422455 7 21.975065904505197 37.94230249994808 20.239587011254727 19.843044584291995 8 22.137221372213723 34.79119406578681 26.25580101954866 16.81578354245081 9 28.289052121290442 20.072508417391866 17.979102867951756 33.659336593365936 10 15.093766322278606 26.051337436451288 29.205676672151338 29.649219569118767 11 28.53897769746928 27.07996310732338 20.21366367509829 24.167395520109046 12 27.062193698860064 25.689256892568924 24.077779239330855 23.170770169240154 13 29.64037332681019 18.424568861073226 33.86495403415572 18.070103777960856 14 30.31522622918537 23.73954508775857 23.992701465476195 21.952527217579867 15 33.341948823985526 20.20909488116777 25.792009747812283 20.656946547034423 16 24.642400270156546 22.36860830146763 31.411237189294972 21.57775423908085 17 19.245577071155324 31.746702082405438 28.023126385110004 20.984594461329227 18 20.093662475086287 21.167519367501367 40.35132659018898 18.387491567223364 19 22.357838963005015 32.22324530937617 24.27916586858176 21.139749859037053 20 20.031815702772413 27.617737715838697 32.73871200250464 19.611734578884253 21 22.257837962995016 30.621921603831424 25.66964131179773 21.45059912137583 22 19.389116968092758 28.87082716981016 23.468619301577633 28.271436560519454 23 24.696323886315785 22.44799371070634 25.620333126408184 27.235349276569686 24 18.624340089554742 28.712287122871228 22.783150908432162 29.880221879141867 25 23.541515809335316 30.164438018125196 25.1616562153477 21.132389957191787 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1711.0 1 1711.0 2 2085.0 3 2459.0 4 2459.0 5 2459.0 6 6979.5 7 11500.0 8 11500.0 9 11500.0 10 8610.5 11 5721.0 12 5721.0 13 5721.0 14 4659.5 15 3598.0 16 3598.0 17 3598.0 18 5883.5 19 8169.0 20 8169.0 21 8169.0 22 12664.5 23 17160.0 24 17160.0 25 17160.0 26 26087.5 27 35015.0 28 35015.0 29 35015.0 30 40003.5 31 44992.0 32 44992.0 33 44992.0 34 50217.0 35 55442.0 36 55442.0 37 55442.0 38 73567.0 39 91692.0 40 91692.0 41 91692.0 42 257994.5 43 424297.0 44 424297.0 45 424297.0 46 328210.0 47 232123.0 48 232123.0 49 232123.0 50 185729.0 51 139335.0 52 139335.0 53 139335.0 54 110888.5 55 82442.0 56 82442.0 57 82442.0 58 68384.5 59 54327.0 60 54327.0 61 54327.0 62 50580.5 63 46834.0 64 46834.0 65 46834.0 66 33159.0 67 19484.0 68 19484.0 69 19484.0 70 17173.0 71 14862.0 72 14862.0 73 14862.0 74 10702.0 75 6542.0 76 6542.0 77 6542.0 78 4149.0 79 1756.0 80 1756.0 81 1756.0 82 1069.5 83 383.0 84 383.0 85 383.0 86 243.0 87 103.0 88 103.0 89 103.0 90 69.5 91 36.0 92 36.0 93 36.0 94 20.0 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12784743232047707 2 0.0 3 4.6154307696923125E-4 4 8.461623077769239E-4 5 0.1033856492411078 6 0.0016923246155538477 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 2.3077153848461562E-4 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.03615420769592311 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1299987.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.424323411406972 #Duplication Level Percentage of deduplicated Percentage of total 1 70.51700877077569 8.761261229731392 2 9.412471931915372 2.338871907658143 3 4.525284806828177 1.6867080590627879 4 2.7616469743281615 1.3724638062874641 5 1.8980469669410578 1.1790974683657889 6 1.4477522902164772 1.0792405603952784 7 1.0888124733260027 0.9469430812083328 8 0.8826013030704664 0.8772579226141356 9 0.7516345365957666 0.8404695512863932 >10 5.632316514858808 13.868350606340341 >50 0.5625144014672855 4.798838777032226 >100 0.37295137045690496 9.90484987934441 >500 0.06792160720241647 5.798222863301452 >1k 0.061746915638560426 14.560229053910723 >5k 0.008027099033012854 6.838266622996844 >10k+ 0.009262037345784064 25.1489286104643 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATAAGCAGTGGTATCAACGCA 74936 5.764365335961052 No Hit GCTTATGTACTCTGCGTTGATACCA 46070 3.5438815926620806 No Hit CTTATGTACTCTGCGTTGATACCAC 33550 2.580795038719618 No Hit GAGTACATAAGCAGTGGTATCAACG 22581 1.7370173701737017 No Hit TATGTACTCTGCGTTGATACCACTG 18435 1.418091103987963 No Hit GCGTTGATACCACTGCTTATGTACT 16495 1.2688588424345781 No Hit GTATCAACGCAGAGTACTTTTTTTT 15820 1.2169352462755396 No Hit CATAAGCAGTGGTATCAACGCAGAG 15674 1.205704364735955 No Hit CCCATGTACTCTGCGTTGATACCAC 14030 1.0792415616463857 No Hit ACGCAGAGTACATAAGCAGTGGTAT 13274 1.0210871339482626 No Hit CTGCTTATGTACTCTGCGTTGATAC 13222 1.0170870939478625 No Hit ACTCTGCGTTGATACCACTGCTTAT 12575 0.9673173654813471 No Hit GGTATCAACGCAGAGTACTTTTTTT 10747 0.8267005746980546 No Hit GTATCAACGCAGAGTACATAAGCAG 10377 0.7982387516182854 No Hit TATCAACGCAGAGTACTTTTTTTTT 10031 0.7716231008463931 No Hit CATGTACTCTGCGTTGATACCACTG 9506 0.7312380816115853 No Hit TATCAACGCAGAGTACATAAGCAGT 9243 0.7110071100711007 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 9161 0.7046993546858545 No Hit GGTATCAACGCAGAGTACATAAGCA 8923 0.6863914792994084 No Hit GTGGTATCAACGCAGAGTACATAAG 6920 0.5323130154378467 No Hit AAGCAGTGGTATCAACGCAGAGTAC 6807 0.5236206208215928 No Hit ACATAAGCAGTGGTATCAACGCAGA 6381 0.49085106235677745 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 5865 0.4511583577374236 No Hit GTACTGGTTCACTATCGGTCAGTCA 5657 0.43515819773582354 No Hit GTGGTATCAACGCAGAGTACATGGG 5394 0.41492722619533884 No Hit ATCATGTACTCTGCGTTGATACCAC 5141 0.3954654931164696 No Hit GATACCACTGCTTATGTACTCTGCG 5088 0.3913885292699081 No Hit CCACTGCTTATGTACTCTGCGTTGA 5051 0.3885423469619312 No Hit GTACATGATAAGCAGTGGTATCAAC 4672 0.35938820926670806 No Hit ATACAGGGTGACAGCCCCGTACACA 4570 0.35154197695823114 No Hit GGATACCACGTGTCCCGCCCTACTC 4527 0.34823425157328497 No Hit GCAGTGGTATCAACGCAGAGTACAT 4480 0.34461883080369266 No Hit GTACATGGGAAGCAGTGGTATCAAC 4245 0.3265417269557311 No Hit ATACCACTGCTTATGTACTCTGCGT 4179 0.3214647531090695 No Hit GTACTCTGCGTTGATACCACTGCTT 4019 0.3091569377232234 No Hit GTTGATACCACTGCTTATGTACTCT 3968 0.30523382156898493 No Hit CAGTGGTATCAACGCAGAGTACATA 3628 0.27907971387406183 No Hit TGATACCACTGCTTATGTACTCTGC 3430 0.2638487923340772 No Hit TACCACTGCTTATGTACTCTGCGTT 3253 0.25023327156348485 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 3109 0.23915623771622332 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 3056 0.23507927386966176 No Hit GTACATGGGATAAGCAGTGGTATCA 2984 0.229540756946031 No Hit GCTTATCCCATGTACTCTGCGTTGA 2978 0.22907921386906177 No Hit GCAGAGTACATAAGCAGTGGTATCA 2815 0.21654062694473097 No Hit CCCATATTCAGACAGGATACCACGT 2558 0.1967711984812156 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 2510 0.19307885386546172 No Hit ATCATTAACTGAATCCATAGGTTAA 2486 0.1912326815575848 No Hit CCCCATGTACTCTGCGTTGATACCA 2402 0.18477107848001556 No Hit ATCAACGCAGAGTACATAAGCAGTG 2278 0.1752325215559848 No Hit GTTAATGATAGTGTGTCGAAACACA 2264 0.17415558770972325 No Hit GTATTTAGCCTTGGAGGATGGTCCC 2257 0.1736171207865925 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 2236 0.17200172001720018 No Hit GCCTTGGAGGATGGTCCCCCCATAT 2195 0.16884784232457709 No Hit GGATAAGCAGTGGTATCAACGCAGA 2107 0.1620785438623617 No Hit GTTCACTATCGGTCAGTCAGGAGTA 2075 0.15961698078519246 No Hit CTATCGGTCAGTCAGGAGTATTTAG 2063 0.158693894631254 No Hit GGTTAATGAGGCGAACCGGGGGAAC 2062 0.15861697078509246 No Hit GCCCAGAGCCTGAATCAGTGTGTGT 2022 0.15554001693863093 No Hit GAGTACATGGGAAGCAGTGGTATCA 2018 0.15523232155398475 No Hit GTGTGTGTGTTAGTGGAAGCGTCTG 1993 0.15330922539994632 No Hit GAGTACATGATAAGCAGTGGTATCA 1990 0.1530784538614617 No Hit TATTCAGACAGGATACCACGTGTCC 1963 0.15100151001510015 No Hit GTACACAAGCAGTGGTATCAACGCA 1958 0.15061689078429247 No Hit CACACACACTGATTCAGGCTCTGGG 1916 0.14738608924550783 No Hit GGTATCAACGCAGAGTACATGGGAA 1895 0.14577068847611552 No Hit GTCAGGAGTATTTAGCCTTGGAGGA 1874 0.14415528770672323 No Hit CATCTAAGTACCCCGAGGAAAAGAA 1816 0.139693704629354 No Hit ACTTAGATGTTTCAGTTCCCCCGGT 1804 0.13877061847541552 No Hit TACATAAGCAGTGGTATCAACGCAG 1781 0.13700137001370014 No Hit GGGATAAGCAGTGGTATCAACGCAG 1779 0.13684752232137706 No Hit ATGTACTCTGCGTTGATACCACTGC 1755 0.13500135001350014 No Hit GAGTACATGGGATAAGCAGTGGTAT 1709 0.13146285309006936 No Hit CCCCAGTAGCGGCGAGCGAACGGGG 1666 0.12815512770512322 No Hit CAGTGGTATCAACGCAGAGTACATG 1657 0.12746281308966936 No Hit GTATCAACGCAGAGTACATGGGAAG 1648 0.1267704984742155 No Hit ATCAACGCAGAGTACTTTTTTTTTT 1634 0.12569356462795397 No Hit GCGTTGATACCACTGCTTCCCATGT 1628 0.12523202155098476 No Hit AAAAAGTACTCTGCGTTGATACCAC 1619 0.12453970693553089 No Hit GCTTATCATGTACTCTGCGTTGATA 1617 0.12438585924320783 No Hit GTGGTATCAACGCAGAGTACTTTTT 1598 0.1229243061661386 No Hit TCACGGTACTGGTTCACTATCGGTC 1536 0.1181550277041232 No Hit GCCTGAATCAGTGTGTGTGTTAGTG 1518 0.11677039847321549 No Hit GATAAGCAGTGGTATCAACGCAGAG 1511 0.11623193155008472 No Hit CGGTACTGGTTCACTATCGGTCAGT 1506 0.11584731231927703 No Hit ACTCTGCGTTGATACCACTGCTTCC 1495 0.11500115001150012 No Hit GGTATCAACGCAGAGTACATGGGAT 1488 0.11446268308836934 No Hit GGTATCAACGCAGAGTACATGGGGG 1482 0.11400114001140012 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1472 0.11323190154978473 No Hit ACGCAGAGTACATGATAAGCAGTGG 1436 0.11046264308796935 No Hit CTGCGTTGATACCACTGCTTATGTA 1427 0.10977032847251549 No Hit GTATCAACGCAGAGTACATGGGATA 1412 0.10861647078009241 No Hit TATCAACGCAGAGTACATGGGAAGC 1410 0.10846262308776934 No Hit GCGTTGATACCACTGCTTATCATGT 1402 0.10784723231847702 No Hit AGCTCACAGCATGTGCATTTTTGTG 1351 0.10392411616423856 No Hit ACGCAGAGTACATGGGATAAGCAGT 1336 0.10277025847181549 No Hit TCATTAACCTATGGATTCAGTTAAT 1301 0.10007792385616164 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.8461923080769267E-4 0.0 0.0 0.0 0.0 2 3.8461923080769267E-4 0.0 0.0 0.0 0.0 3 3.8461923080769267E-4 0.0 0.0 0.0 0.0 4 3.8461923080769267E-4 0.0 0.0 0.0 0.0 5 3.8461923080769267E-4 0.0 0.0 0.0 0.0 6 3.8461923080769267E-4 0.0 0.0 0.0 0.0 7 3.8461923080769267E-4 0.0 0.0 0.0 0.0 8 3.8461923080769267E-4 0.0 0.0 0.0 0.0 9 3.8461923080769267E-4 0.0 0.0 0.0 0.0 10 3.8461923080769267E-4 0.0 0.0 7.692384616153854E-5 0.0 11 3.8461923080769267E-4 0.0 0.0 7.692384616153854E-5 0.0 12 3.8461923080769267E-4 0.0 0.0 7.692384616153854E-5 0.0 13 3.8461923080769267E-4 0.0 0.0 1.5384769232307707E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCTAAC 25 0.006003883 19.015133 2 AGGACTG 25 0.006003883 19.015133 5 GGGTCAC 25 0.0060440577 18.99319 6 GATTGTG 45 3.55023E-5 16.882189 7 CTTATAC 150 0.0 16.49757 1 TGAGCGC 35 0.0021584313 16.298687 5 TATGTCC 35 0.0021584313 16.298687 4 ACAACCC 60 1.4724392E-6 15.827051 11 GATCGAT 80 7.750714E-9 15.431375 11 ACCGTTG 50 8.7417335E-5 15.193969 11 TATACAC 165 0.0 14.981622 3 CCCCTAA 45 6.6658226E-4 14.805513 1 CCTAGTT 45 6.720577E-4 14.789549 2 TATTGAG 45 6.720577E-4 14.789549 2 GGTCCGA 65 3.3688302E-6 14.616333 19 CCCCTGT 40 0.005213227 14.276744 1 GGTAAAC 40 0.005213227 14.276744 1 AAGACTT 40 0.005250678 14.26135 5 GAGCGGC 40 0.005292405 14.244346 17 ACTCGAG 40 0.005292405 14.244346 13 >>END_MODULE