FastQCFastQC Report
Wed 25 May 2016
SRR1295306_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295306_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8036294
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCATTCCATTCCATTCCATTCC384560.47852903340768765No Hit
GAATGGAATGGAATGGAATGGAATG280260.3487428409164722No Hit
GTGGTATCAACGCAGAGTACATGGG93140.11589919433012282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52800.011.219041
TACGTCG803.7668287E-410.6898125
GTGGTAT24700.010.3936581
TGGTATC23000.010.1214392
TATTCCG11950.09.4622985
GTATCAA101950.09.242471
TCTATAC8650.09.1174653
GTCCTAG7600.08.8827281
TAGACTG7150.08.7711285
ACCGGTC1201.7683757E-48.7070728
CTTACAC17000.08.663533
GTCTAAG6050.08.6438841
ACGTCGC1107.161695E-48.6350056
CTAGAAC7750.08.5823953
GTACTAG5700.08.50740051
GTATTAG9150.08.4171591
TCGACCG1252.7504837E-48.3587389
GTCTTAG8300.08.2481391
TATACTG11900.08.2244675
GTCTAGA5900.08.2190141
Produced by FastQC (version 0.11.5)