##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295306_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8036294
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.98166866468549	31.0	30.0	33.0	26.0	34.0
2	29.818398630015277	31.0	30.0	33.0	25.0	34.0
3	30.294544102045048	31.0	30.0	34.0	26.0	34.0
4	33.775986169744414	35.0	35.0	37.0	28.0	37.0
5	34.072566409342414	35.0	35.0	37.0	29.0	37.0
6	34.172522682719176	36.0	35.0	37.0	30.0	37.0
7	34.134320372052095	36.0	35.0	37.0	29.0	37.0
8	34.054679059775566	36.0	35.0	37.0	29.0	37.0
9	35.132024662114155	38.0	35.0	39.0	27.0	39.0
10	35.01581823661504	38.0	35.0	39.0	27.0	39.0
11	35.4135992286992	38.0	35.0	39.0	28.0	39.0
12	35.402398045666324	38.0	35.0	39.0	28.0	39.0
13	35.325357807964714	38.0	35.0	39.0	27.0	39.0
14	35.47654690084758	39.0	34.0	41.0	25.0	41.0
15	36.088326783465114	39.0	35.0	41.0	27.0	41.0
16	36.186400223784744	39.0	35.0	41.0	27.0	41.0
17	35.98696687801616	39.0	35.0	41.0	27.0	41.0
18	35.97023043208723	39.0	35.0	41.0	27.0	41.0
19	36.0362979502741	39.0	35.0	41.0	27.0	41.0
20	35.98892412348279	39.0	34.0	41.0	27.0	41.0
21	35.983664609582476	39.0	34.0	41.0	27.0	41.0
22	35.70293919062692	39.0	34.0	41.0	26.0	41.0
23	35.391304499312746	39.0	34.0	40.0	25.0	41.0
24	35.72118802522655	39.0	34.0	41.0	27.0	41.0
25	34.27499292584368	38.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.38969556937032124
1101	2	0.6083574829593132
1101	3	0.47140822707731544
1101	4	0.45901341665466333
1101	5	0.4161535616968308
1101	6	0.35297215289316597
1101	7	0.4010586645712877
1101	8	0.4013075146531051
1101	9	0.35000331207005075
1101	10	0.6206770098925816
1101	11	0.5696431171060894
1101	12	0.6566996880247729
1101	13	0.5814440430841756
1101	14	1.1044112584691987
1101	15	0.8587891501937861
1101	16	0.7079650991836033
1101	17	0.9981753586189015
1101	18	1.0174795163932657
1101	19	0.9936584783037006
1101	20	1.097823589469158
1101	21	1.1382243406671009
1101	22	0.8563706407071976
1101	23	-1.4331516585990514
1101	24	0.2996052491749168
1101	25	0.7494650250144588
1102	1	0.30845781479900225
1102	2	0.3184557717871428
1102	3	0.36088971138067905
1102	4	0.5207291867297599
1102	5	0.31468799916240187
1102	6	0.2591607273361092
1102	7	0.2279307898882834
1102	8	0.20200186981141144
1102	9	0.40772686736740127
1102	10	0.9463058753210447
1102	11	0.6282768619228634
1102	12	0.49830799462517206
1102	13	0.4827741949208786
1102	14	1.108057175301603
1102	15	0.8556883417772383
1102	16	0.6074648851614199
1102	17	0.9629118970468582
1102	18	0.9434591903705467
1102	19	0.9411299874272885
1102	20	0.9514560935392851
1102	21	1.0530474138986676
1102	22	0.8221165093383149
1102	23	0.9776531415296219
1102	24	0.9856067301343501
1102	25	0.7992997861323659
1103	1	-0.4556399843275898
1103	2	-1.6034855007767135
1103	3	-0.4295348599109978
1103	4	-0.07762028068122362
1103	5	-0.17617764956327875
1103	6	-0.08239838464462679
1103	7	-0.09497811911309384
1103	8	0.021124291688906283
1103	9	0.6416233946626022
1103	10	0.7334393886916999
1103	11	0.41962612475659
1103	12	0.24387230711379004
1103	13	0.06616406109189654
1103	14	-1.1583694688219097
1103	15	-0.24958719719752764
1103	16	-0.14130916753740763
1103	17	0.32658254492227456
1103	18	0.5660819478060688
1103	19	0.4881418181511563
1103	20	0.5156857015370662
1103	21	0.6004527934174746
1103	22	0.20895329237608706
1103	23	1.0322777772503073
1103	24	0.7958892217034048
1103	25	0.6090917821462583
1104	1	-0.048161442117759634
1104	2	0.03641689928157987
1104	3	-0.38044012396697013
1104	4	-0.22799624857268697
1104	5	-0.19738258755663907
1104	6	-0.12418655755909924
1104	7	-0.04571031285986038
1104	8	-0.028437657032846175
1104	9	-2.1763622937944405
1104	10	-0.5835516278650417
1104	11	-0.49719510104230835
1104	12	0.014038524270269193
1104	13	0.1294675457816581
1104	14	-1.7046781642379543
1104	15	-0.48889761974120916
1104	16	-0.08932889490188956
1104	17	0.19944683418955833
1104	18	0.3886437570235941
1104	19	0.3897748497924738
1104	20	0.5306619207827197
1104	21	0.602290365499023
1104	22	0.7481282484889675
1104	23	1.1381289037422917
1104	24	0.7945314196291378
1104	25	0.5374974436629003
1105	1	-0.18810825689573107
1105	2	-0.17137385998512755
1105	3	-1.4430987123028416
1105	4	-0.5491739758341438
1105	5	-0.3796124639290781
1105	6	-0.13136642129168763
1105	7	-0.12269556674500848
1105	8	-0.16444512552963886
1105	9	0.252806652450154
1105	10	0.40137862419146586
1105	11	-0.1650483674712433
1105	12	-0.19992882159012026
1105	13	-0.22918857398182269
1105	14	0.5879653054918066
1105	15	0.1717030910760471
1105	16	-0.3410176700147147
1105	17	0.31602384637750447
1105	18	0.16204601824455978
1105	19	0.18194715871894118
1105	20	-0.37318777640338396
1105	21	-0.001353702404401247
1105	22	0.20751894638718937
1105	23	0.32027510559921524
1105	24	0.256648876873669
1105	25	0.5160776797423381
1106	1	-1.7822316776427378
1106	2	-1.2169714293959295
1106	3	-1.1970069034038637
1106	4	-1.4153908669874866
1106	5	-0.9951700649725481
1106	6	-0.7935553368492876
1106	7	-0.7459493622863604
1106	8	-0.8856579445520438
1106	9	-0.37689008867742047
1106	10	-2.0063268263454503
1106	11	-1.3071109591453904
1106	12	-0.9697915979669105
1106	13	-1.0602149519436992
1106	14	-0.3369695192926372
1106	15	-1.1293982268101104
1106	16	-1.6684586413253655
1106	17	-0.8101047756148958
1106	18	-0.7380203840219579
1106	19	-0.6572070327399544
1106	20	-0.3296021581205295
1106	21	-0.32033226038036844
1106	22	-0.4101343197451399
1106	23	-0.6113816330837878
1106	24	-0.8522040408970781
1106	25	-1.7341917416797017
1107	1	0.07141583608030544
1107	2	0.23432660009642348
1107	3	0.024422783618820887
1107	4	0.15363262374797415
1107	5	-0.04121891330403571
1107	6	0.012455035033269723
1107	7	-0.03597405959811795
1107	8	-0.13387891906415206
1107	9	0.4582119357338925
1107	10	0.011974353819745431
1107	11	0.06427018295924114
1107	12	-0.014214554656938105
1107	13	0.1698734829980637
1107	14	-1.0490586063734284
1107	15	-0.5055003952209702
1107	16	-0.19428328787930127
1107	17	0.0587380442184795
1107	18	0.3604100277623985
1107	19	0.2914008856252437
1107	20	0.3473320043256649
1107	21	0.437528928683534
1107	22	-0.240709194985115
1107	23	0.5746900391553353
1107	24	0.44639355766030775
1107	25	0.13283239769028654
1108	1	-0.19311271460417956
1108	2	0.11772810421878432
1108	3	-0.09408261666911599
1108	4	-0.33039674627143967
1108	5	-0.330739887940382
1108	6	-0.3164112139053685
1108	7	-0.23788951750815102
1108	8	-0.3265217260185196
1108	9	0.21124426228454496
1108	10	0.2149875069912497
1108	11	0.040594447550738266
1108	12	-0.017869352686645357
1108	13	-0.18324665220622194
1108	14	0.018902421020563054
1108	15	-0.10421928974743366
1108	16	-0.1656772831761799
1108	17	0.18491884608922504
1108	18	0.20626663805876433
1108	19	0.21973529058458752
1108	20	0.3184431976507014
1108	21	0.23570138045273126
1108	22	0.20480599295202495
1108	23	-0.019359063715967295
1108	24	0.07043300608521719
1108	25	-0.6842996537955628
1109	1	-0.8847953810525269
1109	2	-0.7094975453752888
1109	3	-0.8482897669649461
1109	4	-0.6964845157478194
1109	5	-1.0234242463601362
1109	6	-1.2814722316880847
1109	7	-0.9798838679199449
1109	8	-1.3818525436961338
1109	9	-0.9903699330210713
1109	10	-1.1311562521842333
1109	11	-1.272394807579829
1109	12	-1.2214791924716835
1109	13	-1.3015424795864021
1109	14	-0.7638027173082236
1109	15	-1.3148004053393691
1109	16	-1.488425671533797
1109	17	-1.4027058179593723
1109	18	-2.1329639829851246
1109	19	-2.23754863019969
1109	20	-1.8908653080709215
1109	21	-1.6866009360456928
1109	22	-1.7606536988955526
1109	23	-1.1967590456400998
1109	24	-2.0721879180786047
1109	25	-1.1485658910430487
1110	1	0.0011061324466474787
1110	2	0.051154711519455276
1110	3	-0.07972236219705664
1110	4	0.16059333104919205
1110	5	0.06425928719801988
1110	6	0.04194487448974371
1110	7	-0.0018437267212974007
1110	8	-0.030940309774727837
1110	9	0.2751394841272372
1110	10	0.2166334739889706
1110	11	0.1281701086945901
1110	12	0.11536848300873714
1110	13	0.10272925822913948
1110	14	-2.616726010831897
1110	15	-0.8090621738963009
1110	16	-0.2596276670748807
1110	17	-0.3707259567868846
1110	18	-3.04588198367113
1110	19	-0.6892803715689269
1110	20	-2.2117088743745867
1110	21	-0.45525438022141174
1110	22	-1.9627868024753852
1110	23	-0.7203099232998795
1110	24	-0.024965317928234754
1110	25	-0.06481236612980723
1111	1	-0.22899503125352183
1111	2	-1.4393390825227712
1111	3	-0.4780520242747066
1111	4	-0.12194593574620427
1111	5	-0.17620348457166557
1111	6	-0.04368325676900753
1111	7	-0.18018783442565223
1111	8	-0.09838362377860221
1111	9	-1.7889884282640551
1111	10	-0.49243586882267465
1111	11	-0.2374201315010538
1111	12	-0.18434542263300813
1111	13	-0.16394711347541602
1111	14	0.7613450960443586
1111	15	0.20905690403277077
1111	16	0.2078055816701081
1111	17	-0.22586553986758418
1111	18	0.023245890746025566
1111	19	-0.25200525738662094
1111	20	0.19051052245390565
1111	21	-0.7506307159163441
1111	22	-0.3943413274956882
1111	23	-0.1589815438544946
1111	24	-0.5792567580546333
1111	25	-2.1507091840168115
1112	1	0.1528327589572207
1112	2	0.34123000926585334
1112	3	-0.06448811015653888
1112	4	0.27270934333483154
1112	5	0.08991969476234374
1112	6	0.06032609287758106
1112	7	0.014034472526773811
1112	8	-0.003882352523106647
1112	9	-0.43459433493905664
1112	10	0.4047370425248218
1112	11	0.057229373604641864
1112	12	8.472219036050888E-4
1112	13	0.023244425165295013
1112	14	0.3651127087536068
1112	15	0.08353596491781445
1112	16	0.13285363723210963
1112	17	-4.057715404590539
1112	18	-1.2268285186176158
1112	19	-1.4333582267156615
1112	20	-0.2570895784637557
1112	21	-0.7150163418885924
1112	22	-1.9193713144017934
1112	23	-1.7699595814621176
1112	24	-0.6069772899479915
1112	25	-0.2290611710466166
1113	1	-0.3551706601551352
1113	2	-0.19119506864817382
1113	3	-0.17580842647196349
1113	4	-0.7004552286199583
1113	5	-0.3244105841796383
1113	6	-0.34939917508405216
1113	7	-0.5326319125298085
1113	8	-0.4214055655086355
1113	9	0.0852496571563961
1113	10	0.24525645191202017
1113	11	-0.2525167137384585
1113	12	-0.18977808039028332
1113	13	-0.5765125464730119
1113	14	-1.805313873193505
1113	15	-2.0699574670169483
1113	16	-0.6911131020357288
1113	17	-0.43733884110326215
1113	18	-0.8478559787373641
1113	19	-0.372173784000303
1113	20	0.08823466827254833
1113	21	-1.867819098132621
1113	22	-2.314391537403658
1113	23	-0.9805708161560602
1113	24	-2.6444011265887895
1113	25	-0.8246998524331914
1114	1	0.07766678603415045
1114	2	0.21329732292934622
1114	3	-0.0608323081122677
1114	4	0.21893913052613811
1114	5	-0.08878288637353648
1114	6	0.11985619731252228
1114	7	0.07514128616983129
1114	8	0.03662558881004685
1114	9	0.4062382583116104
1114	10	0.2767961026630843
1114	11	0.18531662708899432
1114	12	0.1983794004593804
1114	13	0.17698486561913995
1114	14	0.9420293861349407
1114	15	0.40605904470782406
1114	16	0.3068949654172357
1114	17	-0.5379938919560345
1114	18	-0.24203507949398073
1114	19	-0.6739607057166594
1114	20	-1.8434277916192983
1114	21	-0.8721753576865368
1114	22	-1.615756476691459
1114	23	-3.1882284015630162
1114	24	-2.1819842457803915
1114	25	-1.5976122716541994
1115	1	0.41960613731098917
1115	2	0.4076446921312957
1115	3	0.3231371704912611
1115	4	0.47710557830532707
1115	5	0.3961943399269714
1115	6	0.23149344513085168
1115	7	0.346153566599142
1115	8	0.4134718868220588
1115	9	0.6132287425700511
1115	10	0.876623578315538
1115	11	0.5119724589032373
1115	12	0.36807453572274795
1115	13	0.37780688959416153
1115	14	0.9804028347176654
1115	15	0.5101281561156057
1115	16	0.4931560646446016
1115	17	0.45666205330567067
1115	18	0.6993533696740712
1115	19	0.12450171439871127
1115	20	0.6462306922618097
1115	21	-0.5242007409875882
1115	22	0.64279673083918
1115	23	0.6841462790302089
1115	24	0.7284511667101299
1115	25	0.7853383883093557
1116	1	0.47583944246342824
1116	2	0.5879393367390122
1116	3	0.40268625616088016
1116	4	0.6938850776708279
1116	5	0.4377653287821559
1116	6	0.31975322531509676
1116	7	0.30492291749443723
1116	8	0.362881337747865
1116	9	0.6219456780793351
1116	10	0.5302521643611655
1116	11	0.42432707661443914
1116	12	0.4544435635991064
1116	13	-0.16229753278511083
1116	14	0.8795915024227199
1116	15	0.5683509558279027
1116	16	0.576013931350353
1116	17	0.7070385235569532
1116	18	0.6478814277210887
1116	19	0.7862878036566272
1116	20	0.6355507583915383
1116	21	-0.006289729110534381
1116	22	-0.008878894756627176
1116	23	-0.9529516611455762
1116	24	-0.39659305092386177
1116	25	-1.1208597609838478
1201	1	0.43175432675582925
1201	2	0.7054600218100013
1201	3	0.6323031798052696
1201	4	0.5234640446659569
1201	5	0.5041245701653523
1201	6	0.3777943065117242
1201	7	0.4532594219472017
1201	8	0.4244833002600856
1201	9	0.6508763833913918
1201	10	1.0259228852794138
1201	11	0.6263810289079288
1201	12	0.6559468818382967
1201	13	0.6735523880216903
1201	14	1.2522549060666393
1201	15	0.9043983736383367
1201	16	0.5851590352734775
1201	17	0.7220431050738298
1201	18	0.6884197454072805
1201	19	0.9645203291424238
1201	20	1.0198978572867077
1201	21	1.0008712847576007
1201	22	1.1302823125138062
1201	23	0.7210455772879456
1201	24	1.038380036955644
1201	25	1.2426791776908246
1202	1	0.4168764362518438
1202	2	0.7461854970724602
1202	3	0.5976429264496161
1202	4	0.5293119145208109
1202	5	0.4247177607061232
1202	6	0.3087715897604113
1202	7	0.3373118090863656
1202	8	0.34875633842362674
1202	9	0.852441901934192
1202	10	0.0633341150901785
1202	11	0.38834611681943443
1202	12	0.4392297802638794
1202	13	0.33432366876967734
1202	14	1.0986700905715168
1202	15	0.7904699219696383
1202	16	0.7385419753774443
1202	17	-1.389724587532534
1202	18	0.16854778083526156
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2210	18	0.6545263011009013
2210	19	0.8017809269832838
2210	20	0.9112131879895813
2210	21	1.0158748375381208
2210	22	1.1966468492672746
2210	23	1.495650643682815
2210	24	1.161989740150581
2210	25	1.1723317305393195
2211	1	0.06400135841061427
2211	2	-0.6459840469745188
2211	3	-0.10155942674660068
2211	4	0.23662799844977656
2211	5	0.16611448921361927
2211	6	0.12831336969093599
2211	7	0.2175183513629264
2211	8	0.28836061095968546
2211	9	0.7743013491852935
2211	10	0.8068697328998269
2211	11	0.41615653064786073
2211	12	0.28492504503741856
2211	13	0.15043203934990146
2211	14	-0.8757115542712697
2211	15	-0.005837835515691836
2211	16	0.1745513822113196
2211	17	0.2674629476687045
2211	18	0.28299525509540757
2211	19	0.555774792775729
2211	20	0.671550375756027
2211	21	0.6586734670425258
2211	22	-0.13335531240415577
2211	23	0.3976613952812045
2211	24	-0.17373775772659172
2211	25	0.2830001308758625
2212	1	-0.0014169702768569437
2212	2	-1.0127829736607161
2212	3	-0.3458141052427095
2212	4	0.08196298187776563
2212	5	0.09523669593094297
2212	6	0.055665316065898196
2212	7	0.07879356233282664
2212	8	0.1117890543975193
2212	9	-2.2844619702637985
2212	10	-0.047556413447409795
2212	11	0.0021977512593025494
2212	12	-0.028910758464888886
2212	13	-0.007654055560990969
2212	14	0.6405037794640265
2212	15	0.2931477128827211
2212	16	0.3381929838519824
2212	17	0.4265471140332835
2212	18	0.14492189250348986
2212	19	-0.5071271994357929
2212	20	0.31928411954425684
2212	21	0.2884342752908893
2212	22	-1.6998030695517699
2212	23	-0.4029450788809257
2212	24	0.25024237010510575
2212	25	-0.4162097014950845
2213	1	0.21156962928146328
2213	2	0.5319733015126502
2213	3	0.19846857637466186
2213	4	0.37638370356196305
2213	5	0.2765173583916649
2213	6	0.2267941881103397
2213	7	0.19554675029659307
2213	8	0.24041161231814812
2213	9	0.7233092409662163
2213	10	0.7865146088230048
2213	11	0.1410071922050662
2213	12	0.20272411609978747
2213	13	0.1623756658420774
2213	14	-1.7639714368118007
2213	15	-0.35284470727042816
2213	16	-0.013164869031811577
2213	17	-2.0673986667894084
2213	18	-0.8848794872470265
2213	19	-0.5205151080424244
2213	20	0.3257283131705435
2213	21	-0.0735553847659105
2213	22	0.44181871499981895
2213	23	0.9260428024975909
2213	24	0.7407570650958988
2213	25	-0.743350756001341
2214	1	0.3072784854634065
2214	2	0.23317461405092388
2214	3	0.21144088385212712
2214	4	0.49006783087870076
2214	5	0.4054071912777957
2214	6	0.4043148565648238
2214	7	0.30681238925537
2214	8	0.2992231336333191
2214	9	0.7241080869239909
2214	10	0.7156077736919926
2214	11	0.11656662992751876
2214	12	-0.06610935619733027
2214	13	0.17356940592939196
2214	14	1.0279520181992012
2214	15	0.612124532336189
2214	16	0.5854130168203469
2214	17	0.4471986044178493
2214	18	0.45071797237011424
2214	19	-0.7178030986245645
2214	20	0.24378402092690266
2214	21	0.5667822133147098
2214	22	0.687748998171891
2214	23	1.286276004048446
2214	24	1.0105129985835504
2214	25	1.0627787281606516
2215	1	-0.062266580644866565
2215	2	0.41598464305454286
2215	3	0.2856263288650496
2215	4	0.42192109832389946
2215	5	0.3656437178815537
2215	6	0.331557637895493
2215	7	0.2831803493326035
2215	8	0.2657400302798365
2215	9	0.38938080310972367
2215	10	0.8050926335706166
2215	11	0.40622221381673995
2215	12	0.3135812671587743
2215	13	0.18274442849024553
2215	14	1.0752523947510468
2215	15	0.5255083483058414
2215	16	0.5474810948442155
2215	17	0.6651784349071335
2215	18	0.4899949325629436
2215	19	-0.2670832152574576
2215	20	-1.4524455674731556
2215	21	-1.9041549550854455
2215	22	-0.06679332861414622
2215	23	0.5901595632728629
2215	24	0.7492803255520499
2215	25	0.058940125840472035
2216	1	0.1681841338432477
2216	2	-0.09116369876739228
2216	3	0.034900917347819416
2216	4	0.3509994457404204
2216	5	0.36213365320784874
2216	6	0.18542021865831515
2216	7	0.23751157392068478
2216	8	0.2429237336324448
2216	9	0.4440183289521542
2216	10	-1.708842972066222
2216	11	-0.4156425752309829
2216	12	-0.07204350006166038
2216	13	0.14303248073589003
2216	14	-0.3976219652098223
2216	15	-0.3853808872303546
2216	16	0.07397515617417127
2216	17	0.4603772254705092
2216	18	0.2432262163534915
2216	19	-1.2804224149298804
2216	20	-0.9449970760223039
2216	21	-0.8070006907988869
2216	22	-0.6222862143469641
2216	23	-4.220702578934304
2216	24	-1.9850635159687684
2216	25	-1.180701822494882
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9.0
3	40.0
4	93.0
5	140.0
6	235.0
7	653.0
8	358.0
9	938.0
10	2521.0
11	5964.0
12	11485.0
13	18510.0
14	26614.0
15	33547.0
16	39879.0
17	44291.0
18	47713.0
19	50193.0
20	53210.0
21	56413.0
22	60397.0
23	65368.0
24	72179.0
25	79590.0
26	91530.0
27	104745.0
28	123709.0
29	151227.0
30	183342.0
31	226520.0
32	295234.0
33	388506.0
34	517308.0
35	700193.0
36	969412.0
37	1400983.0
38	2213245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.5328200114102	21.08723421505197	12.669267773388635	22.710678000149198
2	15.754021900686451	21.37275948101681	40.55596060753981	22.31725801075693
3	17.52532431264714	27.71108842606809	29.619714892907773	25.143872368376996
4	12.472488731583633	15.742545317656806	37.7605852406153	34.024380710144264
5	15.491957206692392	35.46664933660214	33.299519787895946	15.74187366880952
6	32.88106313532821	37.801692030529985	16.250226361200912	13.067018472940891
7	25.888050355484012	31.80661450926379	25.33501576596031	16.970319369291886
8	23.459694312168768	37.02721378815667	22.07314314765526	17.4399487520193
9	24.34065443648693	15.494108796888465	22.170378257203744	37.99485850942086
10	16.8308879274689	27.790163054578933	31.611436187459557	23.767512830492617
11	34.173803069025375	26.19145443952206	21.56469088631124	18.07005160514132
12	20.946713556241352	26.792473307243377	32.581156086227296	19.679657050287975
13	25.99877147812839	24.82688359350738	27.171891618730044	22.002453309634188
14	21.213367604134156	20.205311094410312	28.099699696858526	30.481621604597002
15	24.12900634921109	28.474040734339468	23.009790462589645	24.387162453859794
16	24.61410163457978	30.85617378262236	23.20686318134718	21.322861401450677
17	20.676658179220656	29.33685277934296	29.187964629791647	20.798524411644742
18	20.647167892072048	30.466961852691455	28.56065445460208	20.325215800634417
19	21.8340083622101	25.3996344364544	29.310997598173326	23.455359603162176
20	24.369384147346235	26.132168838623375	26.70237281520338	22.79607419882701
21	25.377677381023645	28.57861833717039	24.992087968597293	21.05161631320867
22	21.843240529948734	27.531911392762698	29.874816308094843	20.75003176919373
23	21.90315932014378	28.96490695289397	28.33713769378591	20.794796033176347
24	22.129388957453493	24.813979445886265	28.73786062174128	24.318770974918962
25	24.30370660946642	25.990028490915755	26.42332809424039	23.282936805377435
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3055.0
1	3055.0
2	3251.0
3	3447.0
4	3447.0
5	3447.0
6	5102.0
7	6757.0
8	6757.0
9	6757.0
10	10729.0
11	14701.0
12	14701.0
13	14701.0
14	25212.0
15	35723.0
16	35723.0
17	35723.0
18	61522.5
19	87322.0
20	87322.0
21	87322.0
22	134679.5
23	182037.0
24	182037.0
25	182037.0
26	253316.5
27	324596.0
28	324596.0
29	324596.0
30	425859.5
31	527123.0
32	527123.0
33	527123.0
34	667604.5
35	808086.0
36	808086.0
37	808086.0
38	972145.5
39	1136205.0
40	1136205.0
41	1136205.0
42	1120412.5
43	1104620.0
44	1104620.0
45	1104620.0
46	1043491.5
47	982363.0
48	982363.0
49	982363.0
50	923841.5
51	865320.0
52	865320.0
53	865320.0
54	791989.0
55	718658.0
56	718658.0
57	718658.0
58	621532.5
59	524407.0
60	524407.0
61	524407.0
62	436806.0
63	349205.0
64	349205.0
65	349205.0
66	274244.0
67	199283.0
68	199283.0
69	199283.0
70	148802.0
71	98321.0
72	98321.0
73	98321.0
74	70117.0
75	41913.0
76	41913.0
77	41913.0
78	28975.5
79	16038.0
80	16038.0
81	16038.0
82	10625.0
83	5212.0
84	5212.0
85	5212.0
86	3326.5
87	1441.0
88	1441.0
89	1441.0
90	901.0
91	361.0
92	361.0
93	361.0
94	214.0
95	67.0
96	67.0
97	67.0
98	50.0
99	33.0
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08256293261545683
2	0.02065628758728837
3	0.03249010053639152
4	0.040093107594122365
5	0.04989862242471468
6	0.019523924834009308
7	0.023592964617770332
8	0.03978201892563911
9	0.04000600276694705
10	0.026293214260205013
11	0.025098633773229302
12	0.019038626511175428
13	0.02798553661675394
14	0.0424449379278558
15	0.03944604316367718
16	0.026367875540640993
17	0.025820359484110465
18	0.030984431380932556
19	0.019611029661184623
20	0.018043142772028998
21	0.013227490183908154
22	0.02216195674274734
23	0.02058162630685239
24	0.02112914236338292
25	0.10978941288111162
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	8036294.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.245591971891855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.8891712274745	30.716857406682124
2	14.8538635765473	13.441437011812107
3	6.4493646976133645	8.75415970758414
4	3.500525448122761	6.335333844519453
5	2.022619266987353	4.575730303429839
6	1.296672639184443	3.5201232692193276
7	0.8996668630527057	2.8494171857420243
8	0.6082660019815299	2.2017084268824214
9	0.4501453795030781	1.8330384752125815
>10	1.9037898602394305	14.279428463238272
>50	0.07573615663281134	2.3398787509026078
>100	0.04236014869800307	3.7701300311763033
>500	0.004408798529844174	1.3926703434694745
>1k	0.003188151024933908	2.626951510993402
>5k	1.6633830593619606E-4	0.5292210589577054
>10k+	5.5446101978732015E-5	0.8339142101781464
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTCCATTCCATTCCATTCCATTCC	38456	0.47852903340768765	No Hit
GAATGGAATGGAATGGAATGGAATG	28026	0.3487428409164722	No Hit
GTGGTATCAACGCAGAGTACATGGG	9314	0.11589919433012282	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.4887093478660685E-5	0.0
12	0.0	0.0	0.0	2.4887093478660685E-5	0.0
13	0.0	0.0	0.0	2.4887093478660685E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5280	0.0	11.21904	1
TACGTCG	80	3.7668287E-4	10.689812	5
GTGGTAT	2470	0.0	10.393658	1
TGGTATC	2300	0.0	10.121439	2
TATTCCG	1195	0.0	9.462298	5
GTATCAA	10195	0.0	9.24247	1
TCTATAC	865	0.0	9.117465	3
GTCCTAG	760	0.0	8.882728	1
TAGACTG	715	0.0	8.771128	5
ACCGGTC	120	1.7683757E-4	8.707072	8
CTTACAC	1700	0.0	8.66353	3
GTCTAAG	605	0.0	8.643884	1
ACGTCGC	110	7.161695E-4	8.635005	6
CTAGAAC	775	0.0	8.582395	3
GTACTAG	570	0.0	8.5074005	1
GTATTAG	915	0.0	8.417159	1
TCGACCG	125	2.7504837E-4	8.358738	9
GTCTTAG	830	0.0	8.248139	1
TATACTG	1190	0.0	8.224467	5
GTCTAGA	590	0.0	8.219014	1
>>END_MODULE