FastQCFastQC Report
Wed 25 May 2016
SRR1295306_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295306_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8036294
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCATTCCATTCCATTCCATTCC340920.4242253954372501No Hit
GAATGGAATGGAATGGAATGGAATG338040.4206416539763229No Hit
GTATCAACGCAGAGTACTTTTTTTT150110.18679008010408776No Hit
GTGGTATCAACGCAGAGTACATGGG111780.13909396545223457No Hit
GGTATCAACGCAGAGTACTTTTTTT106860.13297174045648405No Hit
TATCAACGCAGAGTACTTTTTTTTT91050.11329849306160276No Hit
GGAATGGAATGGAATGGAATGGAAT89190.11098399336808733No Hit
GGTATCAACGCAGAGTACATGGGGG81460.10136513173858498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64450.011.4178411
GTGGTAT26700.011.0172861
TGGTATC27050.010.2279212
GACCGTA1451.6301965E-79.8234897
CTAACGC1002.7365753E-49.5073983
GTATCAA123300.09.1723551
TATTCCG11100.09.079085
CTAGGAC8700.08.9609963
CTAGCGT850.0073934188.9480824
CTTACAC14700.08.8606363
GTCTTAC6950.08.7664261
GTGTAGA10700.08.719061
GTCCGAT1000.0029176268.54659611
TAGACCG1451.6749931E-58.523825
CGAATGT8900.08.42922615
ACTGTCG1701.623921E-68.3788598
GTATTAG9450.08.3612991
GTCTAGG6300.08.3109291
AGCGCAA2752.3646862E-118.28760710
GTCTTAG6550.08.2843981
Produced by FastQC (version 0.11.5)