FastQCFastQC Report
Wed 25 May 2016
SRR1295305_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295305_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4664957
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCATTCCATTCCATTCCATTCC75010.16079462254421636No Hit
GTGGTATCAACGCAGAGTACATGGG57720.12373104403749059No Hit
GAATGGAATGGAATGGAATGGAATG56350.12079425383770953No Hit
GTATCAACGCAGAGTACTTTTTTTT47690.10223030994712277No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGA700.001494397110.85537810
TAGGACG1158.8121305E-710.74072554
TAGACCG909.509204E-510.5571235
CTAGGAC4150.010.5315713
TGGTATC14700.010.4706062
GGTATCA31650.010.3649921
GTGGTAT16250.09.94761851
CCCCGCG800.00452436979.4984569
GCACCGT1901.0568328E-99.4981516
TGCACCG1356.841863E-69.1495075
GTATTAG5800.09.0169211
GTGTTAC4000.08.7955971
GTCTTAC4000.08.7955971
GTATCAA56000.08.7276771
GTCTTAG4800.08.7163571
GTTCTAG4500.08.6635311
ACGGTCT1107.161413E-48.6348688
TAGGACT5600.08.4834024
CCTAGTA3600.08.4454262
TAGACAG6150.08.3427025
Produced by FastQC (version 0.11.5)