##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295305_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4664957
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.68450212938726	31.0	30.0	33.0	25.0	34.0
2	29.4756646202741	31.0	30.0	33.0	24.0	34.0
3	29.829496391928156	31.0	30.0	34.0	25.0	34.0
4	33.23084800138565	35.0	33.0	37.0	26.0	37.0
5	33.43368502646434	35.0	33.0	37.0	27.0	37.0
6	33.730675974076505	35.0	35.0	37.0	28.0	37.0
7	33.56486994413882	35.0	33.0	37.0	27.0	37.0
8	33.5311536633671	35.0	33.0	37.0	27.0	37.0
9	34.56213122650434	37.0	34.0	39.0	26.0	39.0
10	34.42358825601179	37.0	33.0	39.0	25.0	39.0
11	34.88548597554061	38.0	34.0	39.0	27.0	39.0
12	34.872396251455264	38.0	34.0	39.0	27.0	39.0
13	34.77204848833548	38.0	34.0	39.0	27.0	39.0
14	34.8474191723525	38.0	33.0	40.0	22.0	41.0
15	35.49808090406835	38.0	34.0	40.0	27.0	41.0
16	35.56526630363367	38.0	34.0	40.0	27.0	41.0
17	35.34812475227532	38.0	34.0	40.0	25.0	41.0
18	35.30528534346619	38.0	34.0	40.0	25.0	41.0
19	35.38204210671181	39.0	34.0	40.0	25.0	41.0
20	35.38364490819529	39.0	34.0	40.0	25.0	41.0
21	35.36422950951102	39.0	34.0	40.0	25.0	41.0
22	35.077657521816384	38.0	33.0	40.0	24.0	41.0
23	34.75168559967434	38.0	33.0	40.0	22.0	41.0
24	35.10131883316395	38.0	33.0	40.0	24.0	41.0
25	33.57308373903554	38.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.41922135968230734
1101	2	0.6274593908027875
1101	3	0.4305428128974249
1101	4	0.4221001795446213
1101	5	0.33325995661063956
1101	6	0.3267645848604559
1101	7	0.29078055001259884
1101	8	0.28952487794911974
1101	9	0.1624807676843929
1101	10	0.5705209281368155
1101	11	0.5417512841647607
1101	12	0.568851355144929
1101	13	0.6888550968164182
1101	14	1.0470369545430813
1101	15	0.8685145166839021
1101	16	0.593255847832765
1101	17	0.9347220644476693
1101	18	0.9222812692520819
1101	19	0.89394535276395
1101	20	0.9947858212101437
1101	21	0.9603042521501308
1101	22	0.7282048823080629
1101	23	-1.5197717998135687
1101	24	0.24898295472740983
1101	25	0.6483149406031856
1102	1	0.18128951434479745
1102	2	0.2384051505797018
1102	3	0.1592282780996932
1102	4	0.3491366183255735
1102	5	0.10843199678021165
1102	6	0.12264127098706723
1102	7	0.013066336943694523
1102	8	0.07040486279330338
1102	9	0.21416178418795084
1102	10	0.7922807482611489
1102	11	0.45526048816842035
1102	12	0.4064892240902864
1102	13	0.40887374246435826
1102	14	0.8015500488731107
1102	15	0.5548895667463043
1102	16	0.36983212797614584
1102	17	0.662005782409814
1102	18	0.6957545114178316
1102	19	0.6926384124948299
1102	20	0.8173148158574151
1102	21	0.8615292645722548
1102	22	0.7101786786916051
1102	23	0.7064230089854462
1102	24	0.71210877629089
1102	25	0.6046231355482519
1103	1	-0.5334225380151558
1103	2	-1.7773812976168948
1103	3	-0.672427597202752
1103	4	-0.2443801644667687
1103	5	-0.4425404204779113
1103	6	-0.22835219894978565
1103	7	-0.23329401324745902
1103	8	-0.04883224636036232
1103	9	0.5509832925601259
1103	10	0.5515002537919571
1103	11	0.40128160654345635
1103	12	0.15565368657135537
1103	13	-0.06798936361202834
1103	14	-1.528810825395368
1103	15	-0.5793973334856517
1103	16	-0.45963826277500885
1103	17	0.11014284430692811
1103	18	0.2561840233913628
1103	19	0.21018951563279842
1103	20	0.3546565941517983
1103	21	0.4020954995096986
1103	22	-0.028055785439335068
1103	23	0.7027154232275095
1103	24	0.560375252438277
1103	25	0.35342063192189244
1104	1	0.002053539987056041
1104	2	0.08251094769610745
1104	3	-0.4262853320291846
1104	4	-0.21948680114947905
1104	5	-0.17104724340424582
1104	6	-0.09322729442580169
1104	7	-0.13244436874182242
1104	8	-0.07825668535278396
1104	9	-1.9334879572911632
1104	10	-0.6565684819641433
1104	11	-0.47872867057047586
1104	12	-0.01790181120019696
1104	13	0.14980876085343198
1104	14	-1.8711461794035742
1104	15	-0.6457293997474594
1104	16	-0.16151813757403488
1104	17	0.10281245640788228
1104	18	0.30837905139184585
1104	19	0.22843945333845284
1104	20	0.4237232562715292
1104	21	0.56244529340551
1104	22	0.641976119341578
1104	23	1.089447599390887
1104	24	0.6911636566605708
1104	25	0.5489983339948665
1105	1	-0.4870545543615883
1105	2	-0.4197891108754277
1105	3	-1.6963687550335713
1105	4	-0.960237314236764
1105	5	-0.911416244082524
1105	6	-0.5808184469654094
1105	7	-0.6162705097844565
1105	8	-0.45134036728526183
1105	9	-0.1231790151093719
1105	10	-0.02715759653951011
1105	11	-0.625657386227715
1105	12	-0.6002203840685922
1105	13	-0.6908615329955552
1105	14	-0.010263741201548271
1105	15	-0.2944431322240746
1105	16	-0.764957800292045
1105	17	-0.12613529015155223
1105	18	-0.2589129360032345
1105	19	-0.3036774026615632
1105	20	-0.912281875695804
1105	21	-0.39666255702536546
1105	22	-0.300288310036251
1105	23	-0.2546856622681517
1105	24	-0.2862443198882971
1105	25	-0.15077504919776175
1106	1	-1.8488171467810304
1106	2	-1.2797843807000575
1106	3	-1.3575543998564292
1106	4	-1.6380718383779715
1106	5	-1.2127288087190706
1106	6	-0.9760895308760382
1106	7	-0.7603090721259633
1106	8	-0.8823897972243628
1106	9	-0.535911805062824
1106	10	-2.11958097320786
1106	11	-1.4881234053089756
1106	12	-1.034453237773164
1106	13	-1.168317052819006
1106	14	-0.4567482213466221
1106	15	-1.2460471811282687
1106	16	-1.663724570377191
1106	17	-0.8107358933472923
1106	18	-0.7324786539588573
1106	19	-0.7128600031718832
1106	20	-0.35730814745185313
1106	21	-0.41243485850360173
1106	22	-0.48566829029702063
1106	23	-0.7955335993292678
1106	24	-1.0184939629607541
1106	25	-1.5382179289851905
1107	1	-0.08826556053108447
1107	2	-0.0027545864821227894
1107	3	-0.15927809264963955
1107	4	0.03204169920269351
1107	5	-0.11597537081274112
1107	6	-0.0714475315306018
1107	7	-0.17310578183358416
1107	8	-0.2844247908993722
1107	9	0.39272955929496334
1107	10	-0.008524535495332941
1107	11	-0.08088174070310572
1107	12	-0.23005388116431646
1107	13	-0.06810529042790847
1107	14	-1.2540852809083063
1107	15	-0.6119269853738771
1107	16	-0.40332441172308364
1107	17	-0.07161282006781278
1107	18	0.17892055137004803
1107	19	0.2140514712304551
1107	20	0.25929272551694993
1107	21	0.3311224973929683
1107	22	-0.39271045605882904
1107	23	0.5227074713783679
1107	24	0.39014159909823576
1107	25	-0.09408022086874723
1108	1	-0.16639293029620816
1108	2	0.0933963430377247
1108	3	-0.1347470607827681
1108	4	-0.463659643950173
1108	5	-0.5127227362340889
1108	6	-0.3946373875449112
1108	7	-0.30714640488569955
1108	8	-0.40364312886032394
1108	9	0.15070023118772014
1108	10	0.19816290108145296
1108	11	-0.12413591170154348
1108	12	-0.09000476939288404
1108	13	-0.29696758569784976
1108	14	-0.1392768772569397
1108	15	-0.16441633846488912
1108	16	-0.2247790171555124
1108	17	0.1927716983563741
1108	18	0.13162388763218047
1108	19	0.06129074517043165
1108	20	0.18760391491768758
1108	21	0.1852026521872645
1108	22	0.16658701599400416
1108	23	-0.17283428464212847
1108	24	-0.042346025520515695
1108	25	-0.8723827537866882
1109	1	-0.9586006545308763
1109	2	-0.8110085328919503
1109	3	-0.8794913222141076
1109	4	-0.9379933209782791
1109	5	-1.1744605669094526
1109	6	-1.3298134133516797
1109	7	-1.0981841275676345
1109	8	-1.55346482629378
1109	9	-1.168817488284077
1109	10	-1.3467165266056895
1109	11	-1.3813502306886747
1109	12	-1.37719817010435
1109	13	-1.3888160878341722
1109	14	-0.8485800143004312
1109	15	-1.4495645808237825
1109	16	-1.6508231426791298
1109	17	-1.5734859802558603
1109	18	-2.25555437829685
1109	19	-2.2470060814974886
1109	20	-2.0094431014802154
1109	21	-1.900372548054193
1109	22	-1.9054846434934518
1109	23	-1.459381731094652
1109	24	-2.2702965666217025
1109	25	-1.452367243905897
1110	1	0.11014914193174263
1110	2	0.19147172342462326
1110	3	-0.0740929086680886
1110	4	0.24742588572335933
1110	5	0.1615250536486883
1110	6	0.15571859209264005
1110	7	0.14555846422939567
1110	8	0.13361903735201253
1110	9	0.38085003758914837
1110	10	0.3217065282349765
1110	11	0.29416239353604823
1110	12	0.3629908263414947
1110	13	0.26697833695916273
1110	14	-2.357154012461571
1110	15	-0.5942100086716238
1110	16	-0.15647501323783075
1110	17	-0.24609320597781448
1110	18	-2.812280747774409
1110	19	-0.5043674429874585
1110	20	-2.1226173566895454
1110	21	-0.2992537281739587
1110	22	-1.92181469836936
1110	23	-0.44174028209587846
1110	24	0.22723817748775588
1110	25	0.09828501253737443
1111	1	-0.2594815763992031
1111	2	-1.3695927023456846
1111	3	-0.4147813335707582
1111	4	-0.043727834331640736
1111	5	-0.1814523865485711
1111	6	-0.13892150693762773
1111	7	-0.191033493425941
1111	8	-0.11943096769673645
1111	9	-1.891157456997469
1111	10	-0.48589165122372435
1111	11	-0.2960689638044016
1111	12	-0.19554336615170342
1111	13	-0.0889337475007892
1111	14	0.7909594385263006
1111	15	0.1594930925065441
1111	16	0.211702662817018
1111	17	-0.20395086617585179
1111	18	-0.07844852329583318
1111	19	-0.2663059878758176
1111	20	0.15051607113641552
1111	21	-0.7474065718659517
1111	22	-0.3499817284212554
1111	23	-0.15831114720204909
1111	24	-0.715589919483115
1111	25	-2.1111212421763526
1112	1	0.2981602377410688
1112	2	0.5097765486398593
1112	3	0.15670232664022876
1112	4	0.5201455035748808
1112	5	0.23088521996663758
1112	6	0.24189356142334617
1112	7	0.2883280381123825
1112	8	0.19772515285997372
1112	9	-0.20266820545050734
1112	10	0.578058530271008
1112	11	0.2806484816199841
1112	12	0.2112841340281264
1112	13	0.2529800438179848
1112	14	0.5709146657324382
1112	15	0.36470319305234256
1112	16	0.3587955014507429
1112	17	-3.373350801964083
1112	18	-0.8565566973132235
1112	19	-1.0046504120664608
1112	20	0.029476893187364794
1112	21	-0.3989135307721767
1112	22	-1.7016583397242826
1112	23	-1.2959343294038064
1112	24	-0.14737386619086124
1112	25	0.07418461460333958
1113	1	-0.3339511228460843
1113	2	-0.13940897531156438
1113	3	-0.010856590518780962
1113	4	-0.5425031042453341
1113	5	-0.10330759857230731
1113	6	-0.3167207773559255
1113	7	-0.32446266758291387
1113	8	-0.1611057817094732
1113	9	0.271126463119991
1113	10	0.45511437956448475
1113	11	-0.043043407419510515
1113	12	-0.02267104995758018
1113	13	-0.517777455121454
1113	14	-1.5084940760460555
1113	15	-1.7688267320013793
1113	16	-0.49867688086151674
1113	17	-0.37632330326606933
1113	18	-0.8092363804211686
1113	19	-0.2551337862701786
1113	20	0.3344573892193168
1113	21	-1.6061946672792544
1113	22	-2.009751710444533
1113	23	-0.571150838969551
1113	24	-2.4106838154564016
1113	25	-0.49710868299858646
1114	1	0.22287537225157195
1114	2	0.3521435468259497
1114	3	0.12498505253627457
1114	4	0.5299514075291611
1114	5	0.2803975197988322
1114	6	0.2847919311698206
1114	7	0.2744146720538083
1114	8	0.2346232561369419
1114	9	0.5451051630985972
1114	10	0.4989231803033576
1114	11	0.3261036524783236
1114	12	0.3016486486486798
1114	13	0.25882748218384677
1114	14	1.1679477976461712
1114	15	0.5270938077522302
1114	16	0.5254100933561645
1114	17	-0.28061018042829744
1114	18	-0.01259053371661878
1114	19	-0.44097360301276467
1114	20	-1.5507330429905721
1114	21	-0.6143363682545058
1114	22	-1.2859042440617259
1114	23	-2.9281445721087707
1114	24	-2.0399859553728064
1114	25	-1.4992206829150945
1115	1	0.4988610876485815
1115	2	0.5478874643882108
1115	3	0.45837259871276004
1115	4	0.7431810521169311
1115	5	0.6681989885177799
1115	6	0.5606771870675402
1115	7	0.6962139556850886
1115	8	0.7528074145988342
1115	9	0.866680190545857
1115	10	1.1479302004388359
1115	11	0.818567933619498
1115	12	0.6996229916454269
1115	13	0.6052696841555587
1115	14	1.237290461576535
1115	15	0.7991547057767434
1115	16	0.7948119423135154
1115	17	0.790821976930097
1115	18	1.0472286873025638
1115	19	0.4226349567353722
1115	20	0.9778487003800507
1115	21	-0.19667565243646834
1115	22	0.9828268947207448
1115	23	1.062112139604281
1115	24	1.1021887762562415
1115	25	1.2340891795719386
1116	1	0.5575052053687344
1116	2	0.7074400869500046
1116	3	0.6296820287440177
1116	4	0.9849647183421268
1116	5	0.8773837541352094
1116	6	0.6616815418954118
1116	7	0.7302972855041148
1116	8	0.6874914023544747
1116	9	0.8170474543591268
1116	10	0.816788901722532
1116	11	0.7066085659470787
1116	12	0.7117612417271744
1116	13	0.16945207457948186
1116	14	1.1216063492040504
1116	15	0.8839636751527351
1116	16	0.8828669402764078
1116	17	1.0740425530751097
1116	18	1.096613731940721
1116	19	1.1842894342613093
1116	20	0.9129548195416319
1116	21	0.3249674939128795
1116	22	0.41141666798985455
1116	23	-0.6500412175266632
1116	24	-0.057648134043176924
1116	25	-0.7229005709446596
1201	1	0.43876493611462664
1201	2	0.6263716653152684
1201	3	0.5846897567803282
1201	4	0.46310488688696694
1201	5	0.4469252352266935
1201	6	0.33944915004668985
1201	7	0.3659566068400153
1201	8	0.4134848505959212
1201	9	0.7248606071294219
1201	10	0.9605220220394628
1201	11	0.5860604277756067
1201	12	0.6476242460026072
1201	13	0.6086906731286135
1201	14	1.1763002384519154
1201	15	0.9038866700502552
1201	16	0.6543394371655396
1201	17	0.6356887775223825
1201	18	0.6487511207700152
1201	19	0.9428505408002934
1201	20	1.0196749316209264
1201	21	1.0290245755441632
1201	22	0.9268741000455805
1201	23	0.6201691984822446
1201	24	0.9053158961803973
1201	25	1.0849450474966034
1202	1	0.265622952250979
1202	2	0.6073096182155666
1202	3	0.3949671924365745
1202	4	0.2885172458147949
1202	5	0.23097638158047573
1202	6	0.1609298509080972
1202	7	0.1265107839952222
1202	8	0.1264051426198094
1202	9	0.7208282307012581
1202	10	-0.026674108423357268
1202	11	0.3164468424838205
1202	12	0.19355913929359048
1202	13	0.1965051935733655
1202	14	0.796369782377468
1202	15	0.6693795250011689
1202	16	0.4696971681729636
1202	17	-1.5840794708201429
1202	18	-0.10298143171770846
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2210	17	0.9457165875946387
2210	18	0.8151701271042171
2210	19	1.0156802772652256
2210	20	1.1492028721710597
2210	21	1.1671649992818658
2210	22	1.461582096956107
2210	23	1.746435414462681
2210	24	1.4701020116612895
2210	25	1.3536358102943993
2211	1	0.1800716734025798
2211	2	-0.47573832692818385
2211	3	0.052692555016385256
2211	4	0.43200654497436375
2211	5	0.30287800097324435
2211	6	0.22405999679079258
2211	7	0.30593855480938004
2211	8	0.2913987023242157
2211	9	0.723592024163338
2211	10	0.8715695997160751
2211	11	0.5282306367317844
2211	12	0.39047334907670717
2211	13	0.3411766736084516
2211	14	-0.7471638039533133
2211	15	0.1404180041901668
2211	16	0.4024487299401329
2211	17	0.49807615181206444
2211	18	0.45690219594415993
2211	19	0.7266844433649453
2211	20	0.7334993050720158
2211	21	0.7690929157608366
2211	22	0.11315709930094187
2211	23	0.669446102945777
2211	24	-0.026094952691501305
2211	25	0.48516478782757844
2212	1	0.16318440080683416
2212	2	-0.9779380828161806
2212	3	-0.12034066259946741
2212	4	0.28525763909321
2212	5	0.3534578218495028
2212	6	0.35635855368905567
2212	7	0.286336517247598
2212	8	0.24789326590267535
2212	9	-2.1045779882670033
2212	10	0.05867141538578835
2212	11	0.14293698463590232
2212	12	0.17252396188948893
2212	13	0.27782131260387644
2212	14	0.9181888753835423
2212	15	0.5390821919866227
2212	16	0.5376486957367135
2212	17	0.5286028116503374
2212	18	0.26454225373529994
2212	19	-0.4740460841464653
2212	20	0.48530615616852657
2212	21	0.4724162400279823
2212	22	-1.6006769823200457
2212	23	-0.16682209648369906
2212	24	0.5740011083577485
2212	25	-0.12773081544361986
2213	1	0.23266592730620417
2213	2	0.5199492621764215
2213	3	0.25663270961735307
2213	4	0.4734604880736981
2213	5	0.5583509272334979
2213	6	0.38181824561568334
2213	7	0.3273014288232261
2213	8	0.34583656932111495
2213	9	0.7215618584572923
2213	10	0.8098658904707392
2213	11	0.14252037586056332
2213	12	0.19753454586795982
2213	13	0.24794482999004686
2213	14	-1.567167167842861
2213	15	-0.30532739181762736
2213	16	0.07842238760760267
2213	17	-1.863151890717262
2213	18	-0.8088008808474925
2213	19	-0.391779543120073
2213	20	0.26546739799601937
2213	21	-0.0601392844086277
2213	22	0.499501352541337
2213	23	1.013072673004082
2213	24	0.8081149020192413
2213	25	-0.8053457540398412
2214	1	0.37726762898044797
2214	2	0.4288128846881776
2214	3	0.3411242257545304
2214	4	0.7327562589232812
2214	5	0.6733352769254211
2214	6	0.5295792445365137
2214	7	0.5623795432561636
2214	8	0.46354576360978683
2214	9	0.925377349106725
2214	10	0.8660612051432963
2214	11	0.3772643214420981
2214	12	0.2523370862140908
2214	13	0.3973154632446452
2214	14	1.3812452344423605
2214	15	0.7921436806936129
2214	16	0.7851722390658225
2214	17	0.731045820230463
2214	18	0.7073226397658772
2214	19	-0.564969959568387
2214	20	0.4554226175906777
2214	21	0.8161230677765587
2214	22	1.0318651967450094
2214	23	1.5231083094248845
2214	24	1.2033687623698412
2214	25	1.243921780004051
2215	1	0.15411702965180396
2215	2	0.6002378061288915
2215	3	0.5027735531708863
2215	4	0.7577707513532275
2215	5	0.6870083407488394
2215	6	0.6061741912814682
2215	7	0.514623857618389
2215	8	0.5302479261956847
2215	9	0.68147941476375
2215	10	1.0927851834258675
2215	11	0.7355646267457132
2215	12	0.6681458013258421
2215	13	0.4864777163719438
2215	14	1.4189449559393523
2215	15	0.786357929238001
2215	16	0.8785350947601742
2215	17	0.9710269347338354
2215	18	0.7904739269768442
2215	19	0.2609167497857001
2215	20	-1.2045376728386046
2215	21	-1.5006310745927536
2215	22	0.2465830019537023
2215	23	0.8919328391300141
2215	24	1.0484786917725657
2215	25	0.5307257402408823
2216	1	0.351133743569612
2216	2	0.04218986234999278
2216	3	0.30431937861201064
2216	4	0.6983441066091274
2216	5	0.7328174036569806
2216	6	0.4022433404913528
2216	7	0.5818999325308454
2216	8	0.5412723979589416
2216	9	0.8330907285742342
2216	10	-1.2810529505746189
2216	11	-0.05198400681003079
2216	12	0.3360749662670415
2216	13	0.4002880436667695
2216	14	-0.18045780267795664
2216	15	-0.10400962993210783
2216	16	0.46093675316503635
2216	17	0.7503700123392463
2216	18	0.6770922169673668
2216	19	-0.9998429113782734
2216	20	-0.6400952649170719
2216	21	-0.4078595144719017
2216	22	-0.24099130159221716
2216	23	-4.068005843016408
2216	24	-1.6442754609780863
2216	25	-0.6837355109628547
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	29.0
4	48.0
5	75.0
6	123.0
7	404.0
8	360.0
9	1061.0
10	2739.0
11	5337.0
12	9877.0
13	15327.0
14	21292.0
15	26510.0
16	30781.0
17	33730.0
18	36054.0
19	37325.0
20	38975.0
21	40043.0
22	42483.0
23	45228.0
24	48390.0
25	53243.0
26	59681.0
27	68609.0
28	79363.0
29	96385.0
30	116469.0
31	142040.0
32	182470.0
33	237883.0
34	313600.0
35	415995.0
36	567456.0
37	799241.0
38	1096329.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.377161638710135	20.84241826777127	13.22383855330442	23.55658154021418
2	16.02280285198601	21.480268560443466	39.606254574957255	22.890674012613268
3	18.452554926091523	26.153497778339123	29.658620539550196	25.735326756019155
4	12.918229843754489	16.97776517752559	37.445716812572726	32.658288166147194
5	14.097095057999754	36.89012110394916	34.43246116013234	14.580322677918744
6	31.687997267647678	37.82500552623832	17.317183352976226	13.169813853137777
7	26.225335587809873	32.1781999894509	23.60081871289859	17.995645709840634
8	24.27976461839265	36.56773611390473	21.11801974779807	18.03447951990455
9	24.859961787291166	16.61939756013355	21.801830007054985	36.7188106455203
10	15.782799666795674	29.245969760742568	33.09458693062853	21.876643641833223
11	32.812176367442035	25.5096750455739	23.018816626764046	18.659331960220022
12	21.187194542146887	27.089720321476467	31.48604073021512	20.237044406161527
13	26.56030989740631	24.138046377829625	26.688082968657966	22.613560756106104
14	21.506219821124255	22.544940349375338	27.726705719653538	28.222134109846873
15	23.265392860535577	30.12284953274976	23.84512668690535	22.766630919809312
16	22.983473344370115	30.807781381659026	24.852700555920183	21.356044718050672
17	20.847072949778347	29.680058750060308	28.25309697512235	21.219771325038998
18	20.913991406002076	29.784270925355727	28.729490621220826	20.57224704742137
19	21.582666715264928	27.80036405815497	29.140432538823223	21.476536687756884
20	23.07343827987383	27.716812428926964	28.087507096583863	21.122242194615342
21	23.616570644389412	28.528188914727803	26.85563685034353	20.999603590539255
22	22.1219199742717	27.954010666523732	29.061914078203305	20.86215528100126
23	22.401326911750548	28.457077016326753	28.115636516916688	21.02595955500601
24	22.035379863562746	27.050879029322132	28.56389691350269	22.34984419361243
25	23.05391920167391	27.531262406781483	27.51688395300177	21.89793443854284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2754.0
1	2754.0
2	3141.5
3	3529.0
4	3529.0
5	3529.0
6	5140.0
7	6751.0
8	6751.0
9	6751.0
10	10559.5
11	14368.0
12	14368.0
13	14368.0
14	23859.5
15	33351.0
16	33351.0
17	33351.0
18	53964.0
19	74577.0
20	74577.0
21	74577.0
22	109469.0
23	144361.0
24	144361.0
25	144361.0
26	193230.5
27	242100.0
28	242100.0
29	242100.0
30	299553.0
31	357006.0
32	357006.0
33	357006.0
34	414710.0
35	472414.0
36	472414.0
37	472414.0
38	522151.5
39	571889.0
40	571889.0
41	571889.0
42	573292.5
43	574696.0
44	574696.0
45	574696.0
46	557336.0
47	539976.0
48	539976.0
49	539976.0
50	515923.0
51	491870.0
52	491870.0
53	491870.0
54	452179.5
55	412489.0
56	412489.0
57	412489.0
58	358304.5
59	304120.0
60	304120.0
61	304120.0
62	254099.5
63	204079.0
64	204079.0
65	204079.0
66	159617.5
67	115156.0
68	115156.0
69	115156.0
70	85930.0
71	56704.0
72	56704.0
73	56704.0
74	41125.0
75	25546.0
76	25546.0
77	25546.0
78	18113.5
79	10681.0
80	10681.0
81	10681.0
82	7502.0
83	4323.0
84	4323.0
85	4323.0
86	2959.0
87	1595.0
88	1595.0
89	1595.0
90	1036.0
91	477.0
92	477.0
93	477.0
94	302.0
95	127.0
96	127.0
97	127.0
98	72.5
99	18.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08257310839092408
2	0.020986259894785742
3	0.033312204163939776
4	0.04096500782322324
5	0.05039703474222807
6	0.01809234254463653
7	0.022529682481531987
8	0.033247894889492015
9	0.03401960618286514
10	0.024694761387939912
11	0.02364437657195983
12	0.018263833943163894
13	0.026152438275422477
14	0.03687065068338251
15	0.034512643953631295
16	0.02482337993683543
17	0.02426603289162151
18	0.026516857497293118
19	0.018585380315402696
20	0.015884390788596763
21	0.012604617791760997
22	0.01783510544684549
23	0.019164163785432533
24	0.017899414721293253
25	0.11161946401649575
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4664957.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.3583404528101
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.02627469054194	68.19234460541256
2	9.544870522894074	14.95840428021851
3	1.8357465079021202	4.315381495537547
4	0.5900986486291915	1.8495660324011742
5	0.30091782855506205	1.1789710829118945
6	0.16897144466589195	0.7944193192759876
7	0.10772869468780627	0.5909009214418771
8	0.07727151069170853	0.48438938736670817
9	0.05130220877534216	0.3617960347079463
>10	0.2703916144264794	3.6488334127135897
>50	0.015594093656124306	0.8294853315804407
>100	0.009733952716236543	1.4281733402078396
>500	6.315124751443368E-4	0.3610844181156371
>1k	3.843983152430639E-4	0.5994472294304406
>5k	8.237106755081542E-5	0.4068031086777736
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTCCATTCCATTCCATTCCATTCC	7501	0.16079462254421636	No Hit
GTGGTATCAACGCAGAGTACATGGG	5772	0.12373104403749059	No Hit
GAATGGAATGGAATGGAATGGAATG	5635	0.12079425383770953	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4769	0.10223030994712277	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.143642481592006E-5	0.0
8	0.0	0.0	0.0	4.287284963184012E-5	0.0
9	0.0	0.0	0.0	4.287284963184012E-5	0.0
10	2.143642481592006E-5	0.0	0.0	6.430927444776018E-5	0.0
11	2.143642481592006E-5	0.0	0.0	6.430927444776018E-5	0.0
12	2.143642481592006E-5	0.0	0.0	6.430927444776018E-5	0.0
13	2.143642481592006E-5	0.0	0.0	6.430927444776018E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTGCGA	70	0.0014943971	10.855378	10
TAGGACG	115	8.8121305E-7	10.7407255	4
TAGACCG	90	9.509204E-5	10.557123	5
CTAGGAC	415	0.0	10.531571	3
TGGTATC	1470	0.0	10.470606	2
GGTATCA	3165	0.0	10.364992	1
GTGGTAT	1625	0.0	9.9476185	1
CCCCGCG	80	0.0045243697	9.498456	9
GCACCGT	190	1.0568328E-9	9.498151	6
TGCACCG	135	6.841863E-6	9.149507	5
GTATTAG	580	0.0	9.016921	1
GTGTTAC	400	0.0	8.795597	1
GTCTTAC	400	0.0	8.795597	1
GTATCAA	5600	0.0	8.727677	1
GTCTTAG	480	0.0	8.716357	1
GTTCTAG	450	0.0	8.663531	1
ACGGTCT	110	7.161413E-4	8.634868	8
TAGGACT	560	0.0	8.483402	4
CCTAGTA	360	0.0	8.445426	2
TAGACAG	615	0.0	8.342702	5
>>END_MODULE