FastQCFastQC Report
Wed 25 May 2016
SRR1295305_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295305_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4664957
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT129160.2768728629224235No Hit
GGTATCAACGCAGAGTACTTTTTTT109020.2336999033431605No Hit
TATCAACGCAGAGTACTTTTTTTTT76980.16501759823295264No Hit
GAATGGAATGGAATGGAATGGAATG69980.1500121008618086No Hit
GTGGTATCAACGCAGAGTACATGGG68410.14664658216570914No Hit
ATTCCATTCCATTCCATTCCATTCC67810.14536039667675393No Hit
ACGCAGAGTACTTTTTTTTTTTTTT49340.10576732004174959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44050.011.7370921
CGGTATC657.9647684E-411.7034572
AGCGTCA2250.011.4108713
GAACCGT1105.004895E-711.2221996
GTGGTAT19450.011.014621
TGGTATC18750.010.9544352
AGAACCG1053.4310597E-610.8674965
ACCGAGC1106.0851726E-610.3589538
GCGGTAT750.002599988810.1562961
TATACCG750.002628407210.1429965
TATCCCG951.6296752E-410.0095365
GTCCTAG4450.09.8424771
TAGACAG7200.09.5090595
TTATACG800.0044829279.5090594
TAACCCG2106.548362E-119.5090595
GAACCGA800.0045351879.49570756
TAGACTG6600.09.0768295
GTATTAG5500.09.00217151
TAGGACC2651.8189894E-128.970814
AGCACCG1401.0732221E-58.829845
Produced by FastQC (version 0.11.5)