FastQCFastQC Report
Wed 25 May 2016
SRR1295304_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295304_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4935752
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA657961.3330491483364642No Hit
GCTTATGTACTCTGCGTTGATACCA401320.8130878536847069No Hit
CTTATGTACTCTGCGTTGATACCAC237140.480453637054698No Hit
CCCATGTACTCTGCGTTGATACCAC171500.3474647834818281No Hit
GAGTACATAAGCAGTGGTATCAACG164590.3334648904564087No Hit
TATGTACTCTGCGTTGATACCACTG162000.32821746311403005No Hit
GTATCAACGCAGAGTACTTTTTTTT145030.29383567083597395No Hit
GTACATGATAAGCAGTGGTATCAAC141240.286157003026084No Hit
GTGGTATCAACGCAGAGTACATGGG137390.27835677319281843No Hit
ATCATGTACTCTGCGTTGATACCAC135680.2748922555266148No Hit
GCGTTGATACCACTGCTTATGTACT133730.27094148976690885No Hit
CATGTACTCTGCGTTGATACCACTG121200.2455552872186447No Hit
ACTCTGCGTTGATACCACTGCTTAT107700.21820383195914217No Hit
CATAAGCAGTGGTATCAACGCAGAG100780.2041836785964935No Hit
GTACATGGGAAGCAGTGGTATCAAC91990.18637484217197298No Hit
GTATCAACGCAGAGTACATAAGCAG90420.183193969226979No Hit
GGTATCAACGCAGAGTACTTTTTTT86440.17513035500973306No Hit
CTGCTTATGTACTCTGCGTTGATAC86310.17486697062575265No Hit
ACGCAGAGTACATAAGCAGTGGTAT86030.17429968118333336No Hit
TATCAACGCAGAGTACTTTTTTTTT79440.1609481189492503No Hit
TATCAACGCAGAGTACATAAGCAGT71080.144010477025588No Hit
GGTATCAACGCAGAGTACATAAGCA60960.12350701574957576No Hit
AAGCAGTGGTATCAACGCAGAGTAC58340.11819880739550932No Hit
GTACTGGTTCACTATCGGTCAGTCA55990.11343762814663297No Hit
GCAGTGGTATCAACGCAGAGTACAT52630.10663015483760123No Hit
GAGTACATGATAAGCAGTGGTATCA52290.10594130337180636No Hit
GTGGTATCAACGCAGAGTACATAAG49730.10075465704111551No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG550.003064569312.0938055
TCGAACG600.005840410511.09307519
AGGCGTC600.005885991311.0814957
GGTCCGT1002.4070214E-510.44773813
CGACCGT951.6487307E-49.99803714
CCGTATA1054.0992403E-59.9545642
GTACATA149400.09.6984941
TACATAA149500.09.616492
CGTATAT1002.7514994E-49.5020843
GGCCGTG1106.855225E-59.4984247
CGGCTTA1002.7635702E-49.49794313
ACATAAG152500.09.40239053
CTTACTC4300.09.2811053
GCTTATG95650.09.2242891
CCTAGAC2900.09.1744263
CATAAGC155100.09.1653174
CTTGCTC13800.09.1578053
TACACTG5200.09.1368045
TAGGACG1054.485102E-49.04978754
GTCCTAC3050.09.0399911
Produced by FastQC (version 0.11.5)