FastQCFastQC Report
Wed 25 May 2016
SRR1295304_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295304_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4935752
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA684951.38773179851824No Hit
GCTTATGTACTCTGCGTTGATACCA450110.9119380390262719No Hit
GTATCAACGCAGAGTACTTTTTTTT398250.8068679301553238No Hit
CTTATGTACTCTGCGTTGATACCAC275080.5573213565025147No Hit
GGTATCAACGCAGAGTACTTTTTTT247890.5022334995761537No Hit
TATCAACGCAGAGTACTTTTTTTTT233190.4724508038491399No Hit
CCCATGTACTCTGCGTTGATACCAC205230.4158029009561259No Hit
GAGTACATAAGCAGTGGTATCAACG179910.3645037270916367No Hit
ATCATGTACTCTGCGTTGATACCAC153030.3100439406193828No Hit
TATGTACTCTGCGTTGATACCACTG152650.3092740478046709No Hit
GTGGTATCAACGCAGAGTACATGGG152450.30886884106008566No Hit
GCGTTGATACCACTGCTTATGTACT152080.308119208582603No Hit
CATGTACTCTGCGTTGATACCACTG141090.285853097967645No Hit
GTACATGATAAGCAGTGGTATCAAC137550.2786809385884866No Hit
ACTCTGCGTTGATACCACTGCTTAT126370.2560298815661727No Hit
ACGCAGAGTACTTTTTTTTTTTTTT123040.24928318926882875No Hit
CATAAGCAGTGGTATCAACGCAGAG122460.2481080897095316No Hit
GTACTTTTTTTTTTTTTTTTTTTTT115160.233318043532171No Hit
ACGCAGAGTACATAAGCAGTGGTAT102810.20829652705403348No Hit
CTGCTTATGTACTCTGCGTTGATAC100470.2035556081423864No Hit
GTATCAACGCAGAGTACATAAGCAG97120.19676839517058392No Hit
GTACATGGGAAGCAGTGGTATCAAC89570.1814718405624918No Hit
TATCAACGCAGAGTACATAAGCAGT78080.15819271308607077No Hit
GGTATCAACGCAGAGTACATAAGCA65260.13221896075815803No Hit
AAGCAGTGGTATCAACGCAGAGTAC65060.13181375401357281No Hit
GAGTACTTTTTTTTTTTTTTTTTTT62250.12612059925215044No Hit
GCAGTGGTATCAACGCAGAGTACAT58530.1185837538028653No Hit
GTACTGGTTCACTATCGGTCAGTCA57560.11661850109162697No Hit
GAGTACATGATAAGCAGTGGTATCA55190.11181680116829208No Hit
GTGGTATCAACGCAGAGTACATAAG52940.10725822529170834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG1301.4551915E-1113.1637465
TACGACC951.0317399E-612.0091534
GTCGCTC1053.4873992E-610.85276711
ACGCAAC1408.421921E-910.85276715
TAGCGTT750.002658191110.129257
TACATCG750.002658191110.129257
GTCTTGC14050.09.8922591
GTCTTAC4150.09.86368751
ACGAGGG3000.09.8127115
TATACTG4950.09.7952465
CGAGGGG3050.09.65184616
GTCTCAC11350.09.6454131
TGGACGG1201.6871281E-59.5071515
AGGACCG800.00449043379.507155
GAGCGTT2600.09.4968446
GATACGC1201.71036E-59.49617112
ACCGTCA1002.7687542E-49.496178
CTAGACT4650.09.4050184
GTATTAG4900.09.3253271
AATAGCG1651.0664917E-79.21260719
Produced by FastQC (version 0.11.5)