FastQCFastQC Report
Wed 25 May 2016
SRR1295303_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295303_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3976704
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG47710.11997372698596626No Hit
GTATCAACGCAGAGTACTTTTTTTT43590.10961338837388954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31100.010.4875681
GTCTAAC2251.8189894E-129.7204491
GTGGTAT16700.09.1105491
TGGTATC14850.09.0865042
ACTGCCG850.00745178438.9393068
CTAGGAC3450.08.8139633
GGTCTAC2201.5788828E-98.644671
CTACGTC1000.00289939868.5522993
CTACACT5050.08.4677294
TAATACG1252.7367633E-48.3623534
GTATAAC3200.08.3204941
GTCTTAG3900.08.2900151
CTAGACT3900.08.2843834
AGGATAG4050.08.2121875
AATACTG7650.08.1983855
GTACACG1050.00447613758.1506871
CCGCACC1401.0299243E-48.14955919
CCACGTA1050.004523058.1412569
TCAAGAC6200.08.1231523
GTCCCGG1304.1451634E-48.0461921
Produced by FastQC (version 0.11.5)