##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295303_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3976704
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.701174641109823	31.0	30.0	33.0	25.0	34.0
2	29.516770420931504	31.0	30.0	33.0	24.0	34.0
3	29.98295774591219	31.0	30.0	34.0	25.0	34.0
4	33.23605930941805	35.0	33.0	37.0	26.0	37.0
5	33.54089617934853	35.0	33.0	37.0	27.0	37.0
6	33.563328324159905	35.0	33.0	37.0	27.0	37.0
7	33.532823665025106	35.0	33.0	37.0	27.0	37.0
8	33.47932408346208	35.0	33.0	37.0	27.0	37.0
9	34.452966577346466	37.0	34.0	39.0	25.0	39.0
10	34.36051262553109	37.0	33.0	39.0	25.0	39.0
11	34.77241303350714	38.0	34.0	39.0	27.0	39.0
12	34.766741753975154	38.0	34.0	39.0	27.0	39.0
13	34.659642507966396	38.0	34.0	39.0	27.0	39.0
14	34.75128045738381	38.0	33.0	40.0	20.0	41.0
15	35.37895880608665	38.0	34.0	40.0	25.0	41.0
16	35.46255315960152	38.0	34.0	40.0	26.0	41.0
17	35.25870469615038	38.0	33.0	40.0	25.0	41.0
18	35.23189807438522	38.0	33.0	40.0	25.0	41.0
19	35.31074653783636	39.0	34.0	40.0	25.0	41.0
20	35.29629638011781	39.0	34.0	40.0	25.0	41.0
21	35.272027035454485	39.0	34.0	40.0	25.0	41.0
22	34.99528227396356	39.0	33.0	40.0	24.0	41.0
23	34.68101975907686	38.0	32.0	40.0	21.0	41.0
24	35.018705440485384	38.0	33.0	40.0	24.0	41.0
25	33.516826246057036	38.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.43327551548863497
1101	2	0.573703602320208
1101	3	0.44066873873133616
1101	4	0.24951417868631864
1101	5	0.23175530107762654
1101	6	0.1301144985979903
1101	7	0.24798165084842339
1101	8	0.25399425366528305
1101	9	0.20587724296302667
1101	10	0.40042908566817204
1101	11	0.36294199359203105
1101	12	0.44225220554922373
1101	13	0.4292886637315192
1101	14	0.8810200256247995
1101	15	0.623629388251743
1101	16	0.5051732343470974
1101	17	0.7634644116936897
1101	18	0.803339169734457
1101	19	0.7741956119999998
1101	20	0.9306879333764186
1101	21	0.8998800087180712
1101	22	0.6679996546478364
1101	23	-1.4361502101648966
1101	24	0.17199679531119472
1101	25	0.5510186386627751
1102	1	0.28260867497155573
1102	2	0.3485517191476859
1102	3	0.35022673647128144
1102	4	0.32952806188367134
1102	5	0.1337943180777117
1102	6	0.14013625473553049
1102	7	0.017430619542935233
1102	8	-0.004765919688416886
1102	9	0.18207078814550925
1102	10	0.7808934649601014
1102	11	0.527550221683768
1102	12	0.411094634984579
1102	13	0.35658245851352177
1102	14	0.9091570445869124
1102	15	0.541925952222492
1102	16	0.3837569246946728
1102	17	0.7051687666465654
1102	18	0.6771704603254207
1102	19	0.7148227401153378
1102	20	0.7676045472670623
1102	21	0.8685859407425625
1102	22	0.67652165293417
1102	23	0.7395679534780157
1102	24	0.7617723047549987
1102	25	0.49986409806249554
1103	1	-0.5803028657989735
1103	2	-1.7227566922453832
1103	3	-0.5283016810839811
1103	4	-0.25719671997787685
1103	5	-0.3032023435384943
1103	6	-0.1672056611981816
1103	7	-0.11126986295040098
1103	8	-0.03570846200584299
1103	9	0.6590414033801011
1103	10	0.6845619008748827
1103	11	0.430409186880091
1103	12	0.19514553342592222
1103	13	-0.015011433927625717
1103	14	-1.2326005525390897
1103	15	-0.35788965210120693
1103	16	-0.1994723565204879
1103	17	0.26710318693987034
1103	18	0.3591564651598844
1103	19	0.36812652033469817
1103	20	0.41902805825577616
1103	21	0.5087328169237821
1103	22	0.049431459396849675
1103	23	0.8555820439351933
1103	24	0.6603369458390347
1103	25	0.5960473121224581
1104	1	-0.14964758058786742
1104	2	-0.10611053865484266
1104	3	-0.49890959024875414
1104	4	-0.4162179256632186
1104	5	-0.3706686900052034
1104	6	-0.3168516402252166
1104	7	-0.31055205439449196
1104	8	-0.1953106851291082
1104	9	-2.3073757833825113
1104	10	-0.7338347711075031
1104	11	-0.688508795216876
1104	12	-0.17055814762748156
1104	13	-0.037240722830020445
1104	14	-1.8923384111890869
1104	15	-0.7170380420537228
1104	16	-0.39024926318482045
1104	17	-0.11335542409482002
1104	18	0.10266142988879068
1104	19	0.10401731025108063
1104	20	0.2713573414813979
1104	21	0.36524603278726886
1104	22	0.4873506740889795
1104	23	0.8209476959075346
1104	24	0.5370360244440562
1104	25	0.2952924926945144
1105	1	-0.3904396109876629
1105	2	-0.3026795968927303
1105	3	-1.6231877894504336
1105	4	-0.8090390725843264
1105	5	-0.6135492959685251
1105	6	-0.38705734181230866
1105	7	-0.39297931826549615
1105	8	-0.3090590195384735
1105	9	-0.036325716026816224
1105	10	0.17671462807410876
1105	11	-0.43155581221496675
1105	12	-0.4348169868444387
1105	13	-0.4720556392014075
1105	14	0.3676467622686488
1105	15	0.04823137480088491
1105	16	-0.4211379018784953
1105	17	0.030336992115891803
1105	18	-0.14462542161012948
1105	19	-0.07425856484810822
1105	20	-0.46542814745483696
1105	21	-0.08142115493998858
1105	22	-0.15366788847925505
1105	23	0.16061013907666677
1105	24	-0.0130121951920259
1105	25	0.16614224239702935
1106	1	-1.7816659189861994
1106	2	-1.2067742086483264
1106	3	-1.271156079315979
1106	4	-1.609788693144516
1106	5	-1.220207196691284
1106	6	-0.9763796924158612
1106	7	-0.7076987248987052
1106	8	-0.7885708511030458
1106	9	-0.4802855191842852
1106	10	-2.1878124173928413
1106	11	-1.4833495511008792
1106	12	-1.0389747077739813
1106	13	-1.2523070475034785
1106	14	-0.3444819354686288
1106	15	-1.2121098973104552
1106	16	-1.693358029441491
1106	17	-0.8139568750264132
1106	18	-0.7898639585923632
1106	19	-0.7427005284195474
1106	20	-0.5459119048073262
1106	21	-0.4611994669827624
1106	22	-0.3475409575177366
1106	23	-0.7690233955079293
1106	24	-1.0391124886473477
1106	25	-1.6291607273183004
1107	1	-0.018105292834988518
1107	2	0.10341107676210726
1107	3	-0.05644022690927741
1107	4	0.04810801391492703
1107	5	-0.09580907435113772
1107	6	-0.04792939321343681
1107	7	-0.16172713334384525
1107	8	-0.2680249261913943
1107	9	0.27021014477088556
1107	10	-0.07594818872533438
1107	11	-0.05707967718624474
1107	12	-0.3225639462284846
1107	13	-0.028720659454975817
1107	14	-1.2181809867164048
1107	15	-0.627695935372401
1107	16	-0.36242837313189824
1107	17	-0.1732909173531354
1107	18	0.09470452060779166
1107	19	0.04758603660669536
1107	20	0.07965456828429751
1107	21	0.242411077354582
1107	22	-0.3665687706249159
1107	23	0.3195209490520341
1107	24	0.21222679626156093
1107	25	-0.10928252805327077
1108	1	0.03837100741484889
1108	2	0.2609177322195819
1108	3	0.018152548520081524
1108	4	-0.18594902633813604
1108	5	-0.16682511355555363
1108	6	-0.18261269115185996
1108	7	-0.08625615842363743
1108	8	-0.04215952398937617
1108	9	0.4056329246002761
1108	10	0.3207463476996253
1108	11	0.0751034757226634
1108	12	0.1296140405459809
1108	13	-0.11528858463092462
1108	14	0.06738502060079554
1108	15	0.006740113742210951
1108	16	-0.11188569292092154
1108	17	0.3076659122264829
1108	18	0.3680961914615253
1108	19	0.3836761353019327
1108	20	0.4939783818994954
1108	21	0.36887021401170017
1108	22	0.4272102918927345
1108	23	0.3263548024679537
1108	24	0.2556180132355479
1108	25	-0.612639286785253
1109	1	-0.9542871646235547
1109	2	-0.8613466122303564
1109	3	-0.9318903029932599
1109	4	-0.7579003082446647
1109	5	-1.0106625152572377
1109	6	-1.327689417415641
1109	7	-0.9471139529918631
1109	8	-1.5320630494518106
1109	9	-1.059686193532933
1109	10	-1.2750328997839162
1109	11	-1.3977025213781786
1109	12	-1.3600717068776973
1109	13	-1.327556473736479
1109	14	-0.6970223443930053
1109	15	-1.4395604948699443
1109	16	-1.5819074311745922
1109	17	-1.5699954292444147
1109	18	-2.1094620776411404
1109	19	-2.341475963575796
1109	20	-2.2213055126197574
1109	21	-1.752944744384628
1109	22	-1.9176850777884482
1109	23	-1.342125631554481
1109	24	-2.304448155012892
1109	25	-1.3004159382638818
1110	1	-0.0655790849809108
1110	2	0.11794958925143817
1110	3	-0.07581174792014522
1110	4	0.09328837999693462
1110	5	-0.004420408372304507
1110	6	0.03875173076276894
1110	7	-0.0902185417532877
1110	8	-0.07404477155550637
1110	9	0.13917851701794604
1110	10	0.24272371314329177
1110	11	0.16008662645546679
1110	12	0.1616814076890023
1110	13	0.14287104574328424
1110	14	-2.5085538197121835
1110	15	-0.6189088208475795
1110	16	-0.25162933610335614
1110	17	-0.32453825835457906
1110	18	-2.7987909638712978
1110	19	-0.5664566602228831
1110	20	-2.2485757169132015
1110	21	-0.5935430413864182
1110	22	-2.1554710341173404
1110	23	-0.7091640726657786
1110	24	-0.08683284173250172
1110	25	-0.06145844867181438
1111	1	-0.16119389517547944
1111	2	-1.284051687535431
1111	3	-0.37086893011283095
1111	4	0.013036327875241227
1111	5	-0.07269678608264485
1111	6	0.12861913418077364
1111	7	0.12325355885432288
1111	8	0.0034601896544330657
1111	9	-1.5067967389058552
1111	10	-0.1773389000582597
1111	11	0.02541946400099704
1111	12	-0.04827699678055808
1111	13	0.018553320396399897
1111	14	0.9172737927363528
1111	15	0.4161737638061922
1111	16	0.3905997898307447
1111	17	-0.036424504374615196
1111	18	0.25937107610892696
1111	19	0.027891891930124757
1111	20	0.3808522212625931
1111	21	-0.562814859714841
1111	22	-0.1325411147894826
1111	23	-0.010815232635977168
1111	24	-0.5053814362673776
1111	25	-1.883529535579953
1112	1	0.19421632330875482
1112	2	0.3401719320884986
1112	3	-0.004296606054179364
1112	4	0.4162724537726845
1112	5	0.20873329087267223
1112	6	0.18556357909588428
1112	7	0.173801835399658
1112	8	0.12653328711010658
1112	9	-0.3262438869949591
1112	10	0.5140400093897313
1112	11	0.2003715717699066
1112	12	0.15026656429650842
1112	13	0.17071211012579823
1112	14	0.5310525465318676
1112	15	0.322971931123206
1112	16	0.21756213681366887
1112	17	-3.702926636680864
1112	18	-1.0766828674074134
1112	19	-1.3187491502365845
1112	20	-0.16389457308893185
1112	21	-0.5484936411678163
1112	22	-1.7122951924006742
1112	23	-1.506563086084661
1112	24	-0.4015850011602282
1112	25	0.070301825963341
1113	1	-0.4002050249209326
1113	2	-0.2169061214297976
1113	3	-0.1480578076335668
1113	4	-0.3589261438194953
1113	5	-0.1269746370239062
1113	6	-0.14432511832544037
1113	7	-0.3793498033311806
1113	8	-0.2318126611003848
1113	9	0.24190389109948285
1113	10	0.3810454129455607
1113	11	-0.005541059737410592
1113	12	-0.08036207929382044
1113	13	-0.4828883856988355
1113	14	-1.5090373955912497
1113	15	-1.823435640978758
1113	16	-0.5259257661362255
1113	17	-0.25011306410139866
1113	18	-0.6474350790182513
1113	19	-0.09958487776209779
1113	20	0.26758836951658793
1113	21	-1.6621999088453165
1113	22	-2.1176592716549862
1113	23	-0.7018397155948364
1113	24	-2.5111946742036935
1113	25	-0.581769649953678
1114	1	0.31746293935804104
1114	2	0.38540492496615997
1114	3	0.1693171466968053
1114	4	0.539662597360909
1114	5	0.3416745660503153
1114	6	0.5007964361521857
1114	7	0.46128653261867925
1114	8	0.3970195073586069
1114	9	0.8106522461708536
1114	10	0.6692565659560046
1114	11	0.4126169175523131
1114	12	0.45298324199424655
1114	13	0.4471100755795874
1114	14	1.1909627426030127
1114	15	0.7164166045802389
1114	16	0.6322819744291195
1114	17	-0.21863331763734806
1114	18	0.1281409968490621
1114	19	-0.3175082631516233
1114	20	-1.522506943062858
1114	21	-0.5078356126755779
1114	22	-1.3379911685146126
1114	23	-2.6552943170895347
1114	24	-1.8019156731975912
1114	25	-1.2228033229859747
1115	1	0.40373401095214945
1115	2	0.45225315297706814
1115	3	0.408122026031144
1115	4	0.6324390133682343
1115	5	0.4886547593088153
1115	6	0.46849820534286835
1115	7	0.5451860225479521
1115	8	0.6090113044953185
1115	9	0.7971190975504712
1115	10	0.9738125892409215
1115	11	0.7706034884609494
1115	12	0.6652249938945829
1115	13	0.494447704150069
1115	14	1.2398663921055757
1115	15	0.7646116866659227
1115	16	0.7315433581026198
1115	17	0.6357729229723645
1115	18	0.9103241669601232
1115	19	0.2816409125682071
1115	20	0.8687064949399428
1115	21	-0.2973451742603572
1115	22	0.8488828917777411
1115	23	0.9268139209304351
1115	24	0.9582133360632383
1115	25	0.9952851968634278
1116	1	0.6441785330403071
1116	2	0.8218777508155846
1116	3	0.6361906383500262
1116	4	1.1477384975223615
1116	5	0.8416107919475948
1116	6	0.8189473138872216
1116	7	0.7066117664786944
1116	8	0.7671692677756923
1116	9	1.026644584191125
1116	10	0.9066785170562568
1116	11	0.7422888384693991
1116	12	0.8737645012574475
1116	13	0.2903570093380381
1116	14	1.2812498331667825
1116	15	0.9702905432471809
1116	16	1.0696125322096464
1116	17	1.169160070280988
1116	18	1.145858621782473
1116	19	1.3564982672299521
1116	20	1.118463682552978
1116	21	0.5059150899754741
1116	22	0.4451664966457116
1116	23	-0.5553170247810684
1116	24	0.016269228210568087
1116	25	-0.5763514241296406
1201	1	0.353757134355309
1201	2	0.6497280418219695
1201	3	0.5683489318840991
1201	4	0.3159873531909554
1201	5	0.3973936191842924
1201	6	0.2385932533765711
1201	7	0.1605619209083642
1201	8	0.24350720342221166
1201	9	0.4586388146960658
1201	10	0.8498270608406955
1201	11	0.47168437963738086
1201	12	0.5131211670495475
1201	13	0.43881514564778
1201	14	1.0089832087159323
1201	15	0.6857565907485252
1201	16	0.48593233215138554
1201	17	0.41279613442536345
1201	18	0.3066160603476078
1201	19	0.6664817984581433
1201	20	0.846344764145897
1201	21	0.7184617854698914
1201	22	0.8167659698028302
1201	23	0.434698885058566
1201	24	0.7087399023550915
1201	25	0.919198078519365
1202	1	0.31736693380252845
1202	2	0.6219980914021086
1202	3	0.3734672806408703
1202	4	0.37296346267692115
1202	5	0.1873907432047801
1202	6	0.11827239636599529
1202	7	0.17546099107791235
1202	8	0.14563233380877705
1202	9	0.6683605550407208
1202	10	-0.10839336525513943
1202	11	0.19120320348379494
1202	12	0.2278763686491061
1202	13	0.18325785769422964
1202	14	0.9237872554882927
1202	15	0.6372144011779071
1202	16	0.42645013621935846
1202	17	-1.6145513959717022
1202	18	-0.008837732608562021
1202	19	0.38472023851055326
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2210	20	1.097869838503911
2210	21	1.137830496332846
2210	22	1.2243410817998068
2210	23	1.6048680901628884
2210	24	1.2914200991024956
2210	25	1.2841051537845658
2211	1	0.23123993568643542
2211	2	-0.4867754325842064
2211	3	0.010633861903716735
2211	4	0.33626728555810104
2211	5	0.2844004959923154
2211	6	0.2125355394886057
2211	7	0.24699278273475045
2211	8	0.28568204363981664
2211	9	0.9107528043553188
2211	10	0.9170658861692615
2211	11	0.524877606915517
2211	12	0.3175063689667965
2211	13	0.32506509042558207
2211	14	-0.7529833134052311
2211	15	0.07725499653216161
2211	16	0.25058296115751943
2211	17	0.2586338430563231
2211	18	0.2557490606781272
2211	19	0.5960140317996689
2211	20	0.7509017372806426
2211	21	0.780364902405573
2211	22	0.09098652656773254
2211	23	0.6040327656642503
2211	24	-0.11672560296340606
2211	25	0.49435962162447566
2212	1	0.13949683044719308
2212	2	-0.960677700688553
2212	3	-0.24513487113867782
2212	4	0.34326686113870153
2212	5	0.29144089858825595
2212	6	0.1910501853024229
2212	7	0.2579527699185604
2212	8	0.2808951776213746
2212	9	-1.974300012042228
2212	10	0.1925555248747841
2212	11	0.2610370456556197
2212	12	0.14708274890677586
2212	13	0.20735528674833859
2212	14	0.8774998515069043
2212	15	0.5468586027640256
2212	16	0.5102224727068361
2212	17	0.5971688025200379
2212	18	0.24735553755802187
2212	19	-0.30869217876576727
2212	20	0.6754104119822131
2212	21	0.6135186032419284
2212	22	-1.5440013464470113
2212	23	-0.19753797790200878
2212	24	0.4579901284906782
2212	25	-0.1559379760222086
2213	1	0.3444623691257114
2213	2	0.6358164478475459
2213	3	0.22147308947330302
2213	4	0.5225609951500729
2213	5	0.5686564242855994
2213	6	0.40817696116550195
2213	7	0.33933244342221514
2213	8	0.42492981648037187
2213	9	0.8088335925690657
2213	10	0.8570026847535246
2213	11	0.3252901325433797
2213	12	0.296152332850383
2213	13	0.3757541526372563
2213	14	-1.3541164460350927
2213	15	-0.12406324749153441
2213	16	0.1848178474346014
2213	17	-2.0251429204797944
2213	18	-0.5869278679120029
2213	19	-0.3826748184115658
2213	20	0.36186780043489364
2213	21	0.04278816468877267
2213	22	0.6002064062769321
2213	23	1.0595282814416294
2213	24	0.9329536126544156
2213	25	-0.5070087780080215
2214	1	0.43793431667862137
2214	2	0.3616256998850176
2214	3	0.3419166765694577
2214	4	0.7351859952860451
2214	5	0.6698016981289712
2214	6	0.63770593298851
2214	7	0.5275210190615169
2214	8	0.513363555002222
2214	9	0.793130357011222
2214	10	0.8630002740959739
2214	11	0.2994886275804802
2214	12	0.34923956623952535
2214	13	0.3537932145716738
2214	14	1.1797530509283831
2214	15	0.7779368634572137
2214	16	0.7063635978983029
2214	17	0.6570843114904577
2214	18	0.6005341264025361
2214	19	-0.5021099321902227
2214	20	0.45326358034594705
2214	21	0.6619410843267133
2214	22	0.9845592252149871
2214	23	1.450151154697437
2214	24	1.2389440833901162
2214	25	1.2791421115579737
2215	1	0.11004396155617613
2215	2	0.5828678238191038
2215	3	0.4844868581924544
2215	4	0.8568155630214775
2215	5	0.7002014091677111
2215	6	0.7246525684484695
2215	7	0.7052713462289972
2215	8	0.7315011010305454
2215	9	0.8954092062724897
2215	10	1.1271921181175415
2215	11	0.8799764906065803
2215	12	0.7871809885911745
2215	13	0.5767890015757615
2215	14	1.5258372769808162
2215	15	1.0688038826487016
2215	16	1.0274551139431622
2215	17	1.1317870963860202
2215	18	0.9726827218972502
2215	19	0.20711295000432273
2215	20	-1.1330001163047854
2215	21	-1.4440804057704355
2215	22	0.23764362206758705
2215	23	0.9728948711828309
2215	24	1.0844741004404526
2215	25	0.5148103530492065
2216	1	0.25819170419630666
2216	2	0.017939748220804574
2216	3	0.1858057356255074
2216	4	0.6280229585878416
2216	5	0.6814397931778728
2216	6	0.488476842869737
2216	7	0.5531745281048117
2216	8	0.5662887659424953
2216	9	0.8941362666826862
2216	10	-1.2027347980997192
2216	11	0.04213262078879865
2216	12	0.29538635008959346
2216	13	0.5090872282165577
2216	14	-0.005493259184703447
2216	15	0.13956673006653375
2216	16	0.5347997357699512
2216	17	0.8672228895502414
2216	18	0.7404440502219387
2216	19	-0.9659061500315431
2216	20	-0.4744567461968785
2216	21	-0.3605691259928463
2216	22	-0.26281112104155113
2216	23	-3.7656495321758108
2216	24	-1.6227083923528554
2216	25	-0.5879015917501178
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5.0
3	23.0
4	43.0
5	80.0
6	104.0
7	343.0
8	232.0
9	684.0
10	2057.0
11	4469.0
12	8536.0
13	13934.0
14	19453.0
15	24559.0
16	28909.0
17	32007.0
18	33503.0
19	34476.0
20	35438.0
21	36574.0
22	38171.0
23	39384.0
24	42926.0
25	46585.0
26	51895.0
27	58597.0
28	67276.0
29	80704.0
30	97705.0
31	118539.0
32	151780.0
33	196729.0
34	257073.0
35	341860.0
36	464039.0
37	656385.0
38	991627.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.53655428177964	21.461396133573324	13.657471722577242	23.344577862069798
2	15.84980820709157	21.567855151395797	39.83711373240242	22.745222909110208
3	17.77144832138322	26.458916500814	30.607287388288423	25.162347789514357
4	12.542725243509045	17.671087761367048	38.49117093392911	31.2950160611948
5	13.303996167768803	37.36153699844114	35.74566987059175	13.588796963198302
6	30.305509605636093	38.82949143939796	17.698802323069195	13.166196631896753
7	25.438246786483674	32.73227802811269	24.41376985928565	17.415705326117987
8	23.733023359475013	36.77033870144532	21.726351811105783	17.770286127973893
9	24.521445495335385	17.040784010090164	22.40749698706305	36.0302735075114
10	15.035856551658913	29.888449916907778	34.15549400948713	20.92019952194618
11	31.746767957739657	25.874297017131664	23.922507952613568	18.45642707251511
12	20.68771246419736	27.64335894389386	31.881777601608064	19.787150990300717
13	26.202168803676784	24.450705897504005	27.09658903403923	22.25053626477998
14	21.09706148557307	23.53349731364429	28.25417820255912	27.115262998223518
15	22.575394237016887	31.034199113182105	24.50685312968435	21.883553520116656
16	22.07225290024252	31.370937761431815	25.71135864810793	20.845450690217728
17	20.187629285018634	30.266301985656906	28.72285517663251	20.823213552691946
18	20.230669953016335	30.142993437278104	29.42723392502887	20.199102684676696
19	20.887286241351376	28.831759987182842	29.771718301243915	20.509235470221867
20	22.22651742499771	28.561508365668914	28.95745787487563	20.254516334457744
21	22.72519443363	29.021564502872614	27.75431295087426	20.498928112623123
22	21.54230040712477	28.56890625113967	29.478806347494462	20.4099869942411
23	21.8536831729537	28.968336310033582	28.60414011593411	20.573840401078606
24	21.47221441831582	28.02572681755635	29.11000171280725	21.392057051320585
25	22.208309228946614	28.37913292681883	28.32301942498891	21.089538419245645
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2651.0
1	2651.0
2	3138.0
3	3625.0
4	3625.0
5	3625.0
6	5286.0
7	6947.0
8	6947.0
9	6947.0
10	11044.5
11	15142.0
12	15142.0
13	15142.0
14	25254.5
15	35367.0
16	35367.0
17	35367.0
18	57050.0
19	78733.0
20	78733.0
21	78733.0
22	114063.5
23	149394.0
24	149394.0
25	149394.0
26	196489.0
27	243584.0
28	243584.0
29	243584.0
30	293886.0
31	344188.0
32	344188.0
33	344188.0
34	388542.5
35	432897.0
36	432897.0
37	432897.0
38	461490.0
39	490083.0
40	490083.0
41	490083.0
42	486692.0
43	483301.0
44	483301.0
45	483301.0
46	463011.5
47	442722.0
48	442722.0
49	442722.0
50	418963.5
51	395205.0
52	395205.0
53	395205.0
54	358213.5
55	321222.0
56	321222.0
57	321222.0
58	277259.0
59	233296.0
60	233296.0
61	233296.0
62	192067.0
63	150838.0
64	150838.0
65	150838.0
66	116257.5
67	81677.0
68	81677.0
69	81677.0
70	60232.5
71	38788.0
72	38788.0
73	38788.0
74	27921.0
75	17054.0
76	17054.0
77	17054.0
78	11808.5
79	6563.0
80	6563.0
81	6563.0
82	4483.0
83	2403.0
84	2403.0
85	2403.0
86	1587.5
87	772.0
88	772.0
89	772.0
90	482.0
91	192.0
92	192.0
93	192.0
94	114.5
95	37.0
96	37.0
97	37.0
98	30.0
99	23.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08087099266125916
2	0.02014230880648899
3	0.03238863138921076
4	0.04010859244238445
5	0.05019232007209991
6	0.019035864877043906
7	0.023109590253637183
8	0.033067585618642974
9	0.03387227211278486
10	0.025171599394875756
11	0.024316619994850006
12	0.01860837517703103
13	0.026554654306682118
14	0.03595942770696536
15	0.034375201171623536
16	0.025196745847817687
17	0.024945281318398353
18	0.026831265289043386
19	0.019689672653534183
20	0.017401345435818207
21	0.013579084588644265
22	0.019438208124114842
23	0.020745823677095404
24	0.019488501029998714
25	0.11059410003862495
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3976704.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.39636954544576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.93426531253084	75.00191224801985
2	7.788455782378654	12.990578732312283
3	1.26491044115183	3.164668157765725
4	0.3720697904363788	1.2411707895971522
5	0.18072583103608775	0.7535939095746682
6	0.11023893934433988	0.5516116394315129
7	0.06810900875830748	0.397603084464727
8	0.052260513162570785	0.34866696546723086
9	0.03188993643972578	0.23935544316973328
>10	0.17862461689044937	2.6190571151877102
>50	0.01073698058667764	0.6193287930816485
>100	0.006774517507296598	0.9792042241362591
>500	5.751054243090235E-4	0.3219239849029179
>1k	3.632243526027011E-4	0.7713249128886105
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	4771	0.11997372698596626	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4359	0.10961338837388954	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.5146452941933823E-5	0.0	5.029290588386765E-5	0.0
5	0.0	2.5146452941933823E-5	0.0	5.029290588386765E-5	0.0
6	0.0	2.5146452941933823E-5	0.0	5.029290588386765E-5	0.0
7	0.0	2.5146452941933823E-5	0.0	5.029290588386765E-5	0.0
8	0.0	2.5146452941933823E-5	0.0	5.029290588386765E-5	0.0
9	0.0	2.5146452941933823E-5	0.0	5.029290588386765E-5	0.0
10	0.0	2.5146452941933823E-5	0.0	1.005858117677353E-4	0.0
11	0.0	2.5146452941933823E-5	0.0	1.2573226470966912E-4	0.0
12	0.0	2.5146452941933823E-5	0.0	1.2573226470966912E-4	0.0
13	0.0	2.5146452941933823E-5	0.0	1.2573226470966912E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3110	0.0	10.487568	1
GTCTAAC	225	1.8189894E-12	9.720449	1
GTGGTAT	1670	0.0	9.110549	1
TGGTATC	1485	0.0	9.086504	2
ACTGCCG	85	0.0074517843	8.939306	8
CTAGGAC	345	0.0	8.813963	3
GGTCTAC	220	1.5788828E-9	8.64467	1
CTACGTC	100	0.0028993986	8.552299	3
CTACACT	505	0.0	8.467729	4
TAATACG	125	2.7367633E-4	8.362353	4
GTATAAC	320	0.0	8.320494	1
GTCTTAG	390	0.0	8.290015	1
CTAGACT	390	0.0	8.284383	4
AGGATAG	405	0.0	8.212187	5
AATACTG	765	0.0	8.198385	5
GTACACG	105	0.0044761375	8.150687	1
CCGCACC	140	1.0299243E-4	8.149559	19
CCACGTA	105	0.00452305	8.141256	9
TCAAGAC	620	0.0	8.123152	3
GTCCCGG	130	4.1451634E-4	8.046192	1
>>END_MODULE