FastQCFastQC Report
Wed 25 May 2016
SRR1295303_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295303_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3976704
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT119860.3014053849620188No Hit
GGTATCAACGCAGAGTACTTTTTTT101970.25641838064889916No Hit
TATCAACGCAGAGTACTTTTTTTTT70200.17652809965237543No Hit
GTGGTATCAACGCAGAGTACATGGG57520.14464239732200335No Hit
ACGCAGAGTACTTTTTTTTTTTTTT47230.11876669724475344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA2950.013.2135113
GTCTACG550.003020526612.1171081
GGTATCA42450.011.4381581
CAGCGTC3450.011.298512
TGGTATC17450.010.188322
GTGGTAT18500.010.1381361
GTAGGAC2650.09.6866643
CTAGAAC3650.09.6375193
GAACGGT1002.7677664E-49.4964056
AGACCGT1106.870288E-59.4964046
TTTCGGA800.00453266459.496284510
TAGACAG5650.09.2548765
TAGACTG5550.09.0790265
GCAGCGT3900.09.032351
AGTACCG950.00181208099.0068995
CTTAGAC3700.08.9933753
TAGACGT850.00739240468.94802954
TTAGACT3750.08.8734634
GACCGTG1107.1763713E-48.6329867
CAGCGTA1000.00291677228.54665710
Produced by FastQC (version 0.11.5)