FastQCFastQC Report
Wed 25 May 2016
SRR1295302_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295302_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4857851
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG58640.12071181269248481No Hit
GTATCAACGCAGAGTACTTTTTTTT52790.10866945075095963No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAACG500.001497952813.302823
CCGGTCG655.4652803E-513.1517579
GTCGTAA803.746369E-410.6959361
TAATACG1201.5211881E-610.2940614
GGTATCA37150.010.0321391
TAAGACG900.00111204299.502214
GTGGTAT18100.09.2973891
TAGTACG1151.1087698E-49.089074
TAGACTG6350.08.9784665
GTTAGCC4350.08.7374843
GGTGCGC1201.7680664E-48.707048
ATAGACG1651.048129E-68.6381943
TAGACGG1000.00290058798.5519894
CTTAGAC4500.08.2350793
GTCTACG1856.052833E-78.2227021
TGGTATC18750.08.2095712
GTCTCGC2701.3096724E-108.0989811
GTATCAA62850.08.0477161
GTACCGG1304.1533296E-48.0448071
GGACCGT1304.1953963E-48.0372686
Produced by FastQC (version 0.11.5)