FastQCFastQC Report
Wed 25 May 2016
SRR1295302_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295302_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4857851
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT142840.294039483714095No Hit
GGTATCAACGCAGAGTACTTTTTTT123640.25451583426498675No Hit
TATCAACGCAGAGTACTTTTTTTTT84570.17408932468286903No Hit
GTGGTATCAACGCAGAGTACATGGG69680.14343791112572207No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55890.11505087331826357No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGT600.00589190311.0799956
GGTATCA48700.010.8476811
AGGACCG1502.1136657E-910.774075
AGCGTCA3650.010.6789013
CACCGTC1151.0324002E-59.909147
GTCCTAG4150.09.6332781
GGACCGT1304.2599368E-69.4971396
ACCGTCA800.00453306979.4962598
CTAGACT3950.09.1457134
GTATTAG7200.09.1219871
GTGGTAT21650.09.1009211
TAGACTG5650.09.0858895
CAGCGTC4350.08.9604582
CTGACCG850.00746294958.9377479
GCAGCGT3950.08.9161531
GTGCTAC3650.08.8666371
ACCGTCT1201.7727778E-48.7049048
TGGTATC21300.08.6141662
ACTAGAC3600.08.4505123
CTAGTAC3400.08.3883763
Produced by FastQC (version 0.11.5)