FastQCFastQC Report
Wed 25 May 2016
SRR1295301_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295301_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3777122
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG47380.12543942186670168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG1001.9263916E-611.4026555
GTAGGAC2350.09.7043873
CAGGACC5150.09.4099584
TCTGTCG3050.09.0310618
GTCTTAT4650.08.997421
ACTGTCC4450.08.964588
CGCAGAT1606.7665496E-78.904558
CTTAGAC2252.6193447E-108.86873153
GACTGTC3150.08.7443627
GGTATCA32650.08.6786341
GTCCTAA3950.08.66611
TACGACA1000.00290027458.55199054
GACTGCG1000.0029114238.5483677
AGAGCGC1000.0029114238.5483678
CTGTCGC3350.08.5058379
GTCCAAT3500.08.4219351
TAGGACA4100.08.3434074
AATACGA1150.00110860958.2627935
TCTATAC4900.08.1447543
TTAGACA4550.08.1447534
Produced by FastQC (version 0.11.5)