##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295301_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3777122
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.275722097406437	31.0	30.0	33.0	26.0	34.0
2	30.067749466392666	31.0	30.0	34.0	26.0	34.0
3	30.584232386457202	31.0	30.0	34.0	27.0	34.0
4	33.81617750234173	35.0	35.0	37.0	28.0	37.0
5	34.09863673982466	35.0	35.0	37.0	30.0	37.0
6	34.25784631791083	36.0	35.0	37.0	30.0	37.0
7	34.29525389966223	36.0	35.0	37.0	30.0	37.0
8	34.36246221329361	37.0	35.0	37.0	30.0	37.0
9	35.31937649882636	39.0	35.0	39.0	28.0	39.0
10	35.256086512429306	39.0	35.0	39.0	28.0	39.0
11	35.652463965950794	39.0	35.0	39.0	30.0	39.0
12	35.65919819375704	39.0	35.0	39.0	30.0	39.0
13	35.56297546121095	39.0	35.0	39.0	29.0	39.0
14	35.73678557377813	39.0	34.0	41.0	26.0	41.0
15	36.37774977879984	39.0	36.0	41.0	27.0	41.0
16	36.478795495618094	39.0	36.0	41.0	28.0	41.0
17	36.250098355308616	39.0	36.0	41.0	27.0	41.0
18	36.20050662912133	39.0	36.0	41.0	27.0	41.0
19	36.282645887530244	39.0	36.0	41.0	27.0	41.0
20	36.268277805164885	39.0	35.0	41.0	27.0	41.0
21	36.25495046228319	39.0	35.0	41.0	27.0	41.0
22	35.98674731713723	39.0	34.0	41.0	27.0	41.0
23	35.669867428163556	39.0	34.0	40.0	26.0	41.0
24	36.000613694765484	39.0	34.0	41.0	27.0	41.0
25	34.68898065776006	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4718883992855112
1101	2	0.6935880045896923
1101	3	0.5468302540098691
1101	4	0.46035191028969535
1101	5	0.4493210865340629
1101	6	0.3486033400088715
1101	7	0.39073120300984243
1101	8	0.5073839724142317
1101	9	0.4893803071104941
1101	10	0.8134403830335586
1101	11	0.6430172963857501
1101	12	0.6218833661318399
1101	13	0.6293021750864938
1101	14	1.2036815123227314
1101	15	0.8588979992911732
1101	16	0.6868803360036537
1101	17	0.9838396685715693
1101	18	1.0278842885913306
1101	19	1.010151983537213
1101	20	1.1177770927900497
1101	21	1.0712083144987758
1101	22	0.9200593452230095
1101	23	-1.4678927900890457
1101	24	0.2073134315590366
1101	25	0.6812869030051019
1102	1	0.5697560245830964
1102	2	0.6164348148299723
1102	3	0.6435685505546473
1102	4	0.7803109200433198
1102	5	0.505226479087149
1102	6	0.3847603433390603
1102	7	0.4646700771379244
1102	8	0.4964605154636601
1102	9	0.8481054685639435
1102	10	1.281614784812021
1102	11	0.9399069842074113
1102	12	0.8053615207651177
1102	13	0.8887554822769559
1102	14	1.5491255123589838
1102	15	1.1709322022927395
1102	16	1.0533714146490993
1102	17	1.311991340108527
1102	18	1.2940039950078486
1102	19	1.2625700555379709
1102	20	1.3030697822236448
1102	21	1.3066981695481772
1102	22	1.1482262177747344
1102	23	1.2501807412696877
1102	24	1.3027960281376991
1102	25	1.2483569739917968
1103	1	-0.1789808284136143
1103	2	-1.556702270445605
1103	3	-0.20490019051267083
1103	4	0.14105443786403526
1103	5	-0.007472815568270619
1103	6	0.019005454975427938
1103	7	0.1842710277910058
1103	8	0.4237658079442781
1103	9	1.1516479857542095
1103	10	1.1145693204624223
1103	11	0.8309560170012134
1103	12	0.6233924982917429
1103	13	0.45536389030081637
1103	14	-0.5661612003314502
1103	15	0.16957114411779628
1103	16	0.2786343673099694
1103	17	0.6953474107491644
1103	18	0.8832203901272706
1103	19	0.9256341452720207
1103	20	0.9336545054107077
1103	21	1.086212255563531
1103	22	0.6215198160813031
1103	23	1.433990254463339
1103	24	1.304600748769218
1103	25	1.1117299711895754
1104	1	0.13402494687503008
1104	2	0.2470369781242674
1104	3	-0.14998217732739505
1104	4	-0.16494548171023382
1104	5	-0.16871464223692811
1104	6	-0.0390866356886832
1104	7	0.004418995097545064
1104	8	0.1019327078945409
1104	9	-2.1016708780734064
1104	10	-0.46115136820090186
1104	11	-0.37045572170815433
1104	12	0.12641006623645268
1104	13	0.2753763896879349
1104	14	-1.6186009788961755
1104	15	-0.41444094272008414
1104	16	-0.07700465851270621
1104	17	0.2773226692838122
1104	18	0.36375168622259935
1104	19	0.41087041321367224
1104	20	0.675910957404362
1104	21	0.8617365023839412
1104	22	0.8498747746279847
1104	23	1.2243213185531587
1104	24	0.8656469485400393
1104	25	0.9108567642190479
1105	1	-0.21208267091998323
1105	2	-0.07541367735370486
1105	3	-1.4843093914570709
1105	4	-0.5694071246354753
1105	5	-0.4532313177801015
1105	6	-0.19652528081594056
1105	7	-0.1316534578135773
1105	8	-0.11217731454849655
1105	9	0.3302399844497117
1105	10	0.49968571122414573
1105	11	-0.14198838063327202
1105	12	-0.14560820114865436
1105	13	-0.1998595767109208
1105	14	0.6702328750833288
1105	15	0.23675491080880562
1105	16	-0.3002596517406104
1105	17	0.3290392291316522
1105	18	0.24492951520283412
1105	19	0.36099980998420733
1105	20	-0.3956471709916727
1105	21	0.07950163880285999
1105	22	0.2251834759582252
1105	23	0.36063087213108247
1105	24	0.3734069523965502
1105	25	0.4658164281045245
1106	1	-1.5624987399700316
1106	2	-0.9962788027667671
1106	3	-1.0638851883565188
1106	4	-1.2748492372625577
1106	5	-0.8755597080054116
1106	6	-0.8319443980269341
1106	7	-0.5849024825847664
1106	8	-0.657234269765965
1106	9	-0.2747973720344703
1106	10	-1.7549942487408074
1106	11	-1.2358198809945833
1106	12	-0.9315364835008282
1106	13	-1.0051087234647653
1106	14	-0.2812665137386219
1106	15	-0.9150761078053549
1106	16	-1.4057489975116724
1106	17	-0.5349063390615711
1106	18	-0.42219198654412793
1106	19	-0.4193372261128232
1106	20	-0.1216392848414003
1106	21	-0.23181459897111978
1106	22	-0.17994335789213523
1106	23	-0.6080062710802068
1106	24	-0.8183843108349791
1106	25	-1.4987918797009883
1107	1	-0.13064533787891008
1107	2	0.013372726657774336
1107	3	-0.15568580996039216
1107	4	0.0062815370544484495
1107	5	-0.1598767954466922
1107	6	-0.04687913819174838
1107	7	-0.11813879305766761
1107	8	-0.2434439874165193
1107	9	0.30485687400457806
1107	10	-0.21107730000230873
1107	11	-0.1334641214232164
1107	12	-0.1983214607441468
1107	13	-0.17479046762978356
1107	14	-1.3138295554104147
1107	15	-0.7318166322290125
1107	16	-0.5324289792354548
1107	17	-0.13211313227373012
1107	18	0.1574858627619946
1107	19	0.030067411858794912
1107	20	0.16222831707754182
1107	21	0.21093564475339832
1107	22	-0.5222034916094387
1107	23	0.3936000943563229
1107	24	0.2336079616216864
1107	25	-0.2781714387215999
1108	1	-0.23709683219701105
1108	2	0.007163065495671361
1108	3	-0.29128749203803395
1108	4	-0.5404243311342469
1108	5	-0.5079358712982369
1108	6	-0.4457858903374401
1108	7	-0.3300814028503112
1108	8	-0.3548571983340949
1108	9	0.179756197360895
1108	10	0.11843929121467767
1108	11	-0.1205824175318213
1108	12	-0.22750352829932297
1108	13	-0.326240492737611
1108	14	-0.12504178912259079
1108	15	-0.33531077738000903
1108	16	-0.36853128867260665
1108	17	0.022878165289242247
1108	18	0.07387250610394602
1108	19	0.010772140643680927
1108	20	0.049530848517619575
1108	21	0.048533913600799394
1108	22	0.026035620406752003
1108	23	-0.12790350026497777
1108	24	-0.021490900364248944
1108	25	-0.9262656670290568
1109	1	-1.0376751424102864
1109	2	-0.976019480823286
1109	3	-1.0137613547325657
1109	4	-0.979331576355591
1109	5	-1.2005733251840311
1109	6	-1.510932239572746
1109	7	-1.1448910345186434
1109	8	-1.5712564520332037
1109	9	-1.2296832097614114
1109	10	-1.4829967807462694
1109	11	-1.6300450250366652
1109	12	-1.4772986973128326
1109	13	-1.5152289470338545
1109	14	-1.048812557584398
1109	15	-1.64661548030152
1109	16	-1.7917457198520665
1109	17	-1.7042040045759776
1109	18	-2.363818968932641
1109	19	-2.440545156034446
1109	20	-2.161970770132612
1109	21	-2.0286997395223594
1109	22	-2.0918131169671668
1109	23	-1.5863565713733934
1109	24	-2.4899624005238863
1109	25	-1.676145610142349
1110	1	-0.12385102481254862
1110	2	0.05310265082564314
1110	3	-0.1566913068728013
1110	4	0.05840921129311738
1110	5	0.06488675745163874
1110	6	-0.007864175489544323
1110	7	0.0017456698181561592
1110	8	-0.09078218000123428
1110	9	0.07869091618426438
1110	10	0.07424006322305132
1110	11	0.018482977915070364
1110	12	-0.01780600535301602
1110	13	0.027434264604274006
1110	14	-2.8325998277707995
1110	15	-1.036876925555788
1110	16	-0.46209269395792774
1110	17	-0.5405626916105675
1110	18	-3.0431974048490744
1110	19	-0.6930846397887009
1110	20	-2.41213345261324
1110	21	-0.6894374548569289
1110	22	-2.1473806356272434
1110	23	-0.7932377123681249
1110	24	-0.18721046147778964
1110	25	-0.2848772177873613
1111	1	-0.35124655896732193
1111	2	-1.513264530601198
1111	3	-0.6120198321347452
1111	4	-0.12786141662094508
1111	5	-0.29363196387838997
1111	6	-0.08446099159306186
1111	7	-0.2765377626436347
1111	8	-0.3668708658513822
1111	9	-2.0686088164243657
1111	10	-0.7281683514729096
1111	11	-0.4806631415535989
1111	12	-0.362284538398562
1111	13	-0.1942361391540004
1111	14	0.6398431096638859
1111	15	0.03197483333402573
1111	16	0.05223672758850029
1111	17	-0.4321321894133803
1111	18	-0.23182856062977208
1111	19	-0.3979882042998213
1111	20	0.05320402254571377
1111	21	-0.7449798772791212
1111	22	-0.5766566291967834
1111	23	-0.4849707056465391
1111	24	-0.7729395638517218
1111	25	-2.4542847945633994
1112	1	0.06778690616192762
1112	2	0.2744680145987708
1112	3	-0.07967709121972177
1112	4	0.31805887500173924
1112	5	0.11335608153466126
1112	6	0.10708237199622062
1112	7	-0.009161115166271827
1112	8	-0.06994093496788878
1112	9	-0.457329978758402
1112	10	0.36608814585456173
1112	11	-0.09713546121601979
1112	12	-0.034051404137002805
1112	13	-0.11625573139723855
1112	14	0.3563659001120669
1112	15	0.04526066588059763
1112	16	0.01944354861377917
1112	17	-4.1828991591952445
1112	18	-1.3077695505538216
1112	19	-1.444885847368944
1112	20	-0.3064466643492807
1112	21	-0.7560270206707855
1112	22	-1.9307193834720096
1112	23	-1.7369956309046444
1112	24	-0.5956504020166093
1112	25	-0.28686457716543856
1113	1	-0.48223833327473287
1113	2	-0.2286167821371059
1113	3	-0.32393010196095773
1113	4	-0.7481191228163269
1113	5	-0.3849701913928456
1113	6	-0.3536503021685391
1113	7	-0.5303732081191441
1113	8	-0.5309975364121797
1113	9	0.018441457265012673
1113	10	0.1481711630627487
1113	11	-0.3075845263157575
1113	12	-0.39719820987973975
1113	13	-0.6207330453734841
1113	14	-1.876055574228758
1113	15	-2.0909219643689454
1113	16	-0.8597532864020678
1113	17	-0.6865510383718956
1113	18	-0.9155331129486441
1113	19	-0.4981461779045375
1113	20	-0.12619719396298024
1113	21	-1.914539909756975
1113	22	-2.312713403325418
1113	23	-1.0552696516235045
1113	24	-2.857629981571833
1113	25	-1.036749198117107
1114	1	-0.14452136582143638
1114	2	0.042044967984782744
1114	3	-0.26843738737752076
1114	4	0.0854418156653054
1114	5	-0.16892014464072957
1114	6	-0.06520242369864349
1114	7	-0.1124502675329282
1114	8	-0.21886697342286254
1114	9	0.016725576297766054
1114	10	-0.002839405749824664
1114	11	-0.17799981411243238
1114	12	-0.14384844735315028
1114	13	-0.16308658226662232
1114	14	0.6208794750099713
1114	15	0.03281201557991409
1114	16	0.028944254250781398
1114	17	-0.7506276522482409
1114	18	-0.5073730604612052
1114	19	-0.8593082587598104
1114	20	-2.0375442070545517
1114	21	-1.1996365112637903
1114	22	-1.9181805434733263
1114	23	-3.387739096646037
1114	24	-2.408954356280546
1114	25	-1.9587047166748164
1115	1	0.15487554496461442
1115	2	0.18362405750740862
1115	3	0.09713276267639515
1115	4	0.29213717717360055
1115	5	0.2236792059176338
1115	6	0.07798950681637962
1115	7	0.1894070279223996
1115	8	0.18384913650701407
1115	9	0.31802786112199044
1115	10	0.5822678089822233
1115	11	0.211121429208859
1115	12	0.13577461046502748
1115	13	0.007701794799430672
1115	14	0.6848416071605783
1115	15	0.2017122801898097
1115	16	0.12216527560843815
1115	17	0.25559631576300745
1115	18	0.41199834379894895
1115	19	-0.30650863323459987
1115	20	0.36408543859563025
1115	21	-0.7232259353971102
1115	22	0.290014087971592
1115	23	0.3879077049858921
1115	24	0.4768625633190666
1115	25	0.46783669182966037
1116	1	0.12937602151093586
1116	2	0.3400943509563845
1116	3	0.18533412754190337
1116	4	0.538780703461704
1116	5	0.3327084170293375
1116	6	0.21384724562105362
1116	7	0.14327074317235144
1116	8	0.0973814697749944
1116	9	0.26146462003312365
1116	10	0.20713741040434286
1116	11	0.12503495820633503
1116	12	0.11931813133909941
1116	13	-0.5570605935894477
1116	14	0.5690403784899587
1116	15	0.16251778428654973
1116	16	0.19152899431226444
1116	17	0.40345237686956636
1116	18	0.3069159682667504
1116	19	0.5383662842044785
1116	20	0.308520662864062
1116	21	-0.17922308326308922
1116	22	-0.34355866095884124
1116	23	-1.128840139574102
1116	24	-0.6929413740168755
1116	25	-1.4990187335866025
1201	1	0.5374840971542767
1201	2	0.7848551104506178
1201	3	0.6924441869388431
1201	4	0.5188355936879248
1201	5	0.5509738093601015
1201	6	0.422528069499414
1201	7	0.5022159859675099
1201	8	0.5824937972086701
1201	9	0.8823857241194588
1201	10	1.211279402495137
1201	11	0.8384771021372472
1201	12	0.8057632529024019
1201	13	0.7796941029772171
1201	14	1.3870423084121626
1201	15	1.05921525051847
1201	16	0.7834749280177888
1201	17	0.8701664685493284
1201	18	0.7784626064962055
1201	19	1.0817581691885891
1201	20	1.1875736218334225
1201	21	1.0788447967898875
1201	22	1.221608512212299
1201	23	0.754512123724588
1201	24	1.1298737053577312
1201	25	1.4097232623302176
1202	1	0.5224201106909234
1202	2	0.9137614659816933
1202	3	0.7320423273589576
1202	4	0.6061135873176724
1202	5	0.4992487303384294
1202	6	0.41224414283504984
1202	7	0.5233039791396408
1202	8	0.5760860812312671
1202	9	1.1837568389907531
1202	10	0.3883023324251198
1202	11	0.5852147876858567
1202	12	0.6315245928496793
1202	13	0.6034324716435648
1202	14	1.3197303187862914
1202	15	1.0506468645894884
1202	16	0.8997206695589028
1202	17	-1.2025675681912702
1202	18	0.36420758249335705
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2210	16	0.09761756904511287
2210	17	0.564582057805687
2210	18	0.516592841720616
2210	19	0.6716753053143876
2210	20	0.8149224277170504
2210	21	0.790076084296814
2210	22	1.0361125821433959
2210	23	1.3849814029104195
2210	24	1.0967904931647823
2210	25	0.866739571616705
2211	1	0.153732281617458
2211	2	-0.5876167368055683
2211	3	-0.0719560813764879
2211	4	0.25317482593690954
2211	5	0.28327551561667974
2211	6	0.1846226511693061
2211	7	0.18735851430844264
2211	8	0.22227151930225375
2211	9	0.7379092097026643
2211	10	0.7710793570197083
2211	11	0.4354266393654811
2211	12	0.27812264901760386
2211	13	0.23297640277577614
2211	14	-0.8897226872645803
2211	15	0.008425952814775428
2211	16	0.23584300158692173
2211	17	0.2679171105932667
2211	18	0.2277867911223126
2211	19	0.5532741559666903
2211	20	0.6447710620102924
2211	21	0.63410754627958
2211	22	0.013097569494327388
2211	23	0.5188435859658043
2211	24	-0.10706306575288949
2211	25	0.37861770932685346
2212	1	-0.01672113092075378
2212	2	-1.0346842961671463
2212	3	-0.33510341358510587
2212	4	0.1679895520365804
2212	5	0.2202824542436943
2212	6	0.1856668763386935
2212	7	0.144398974311315
2212	8	0.12878488188856352
2212	9	-2.211591853943929
2212	10	-0.14622907977586408
2212	11	0.09673126697264678
2212	12	0.07890093016988686
2212	13	0.07053109026566062
2212	14	0.6364062476775416
2212	15	0.30196457490366413
2212	16	0.32048253350473743
2212	17	0.35913144153172283
2212	18	0.163452033119718
2212	19	-0.5722665688149178
2212	20	0.26866452644254224
2212	21	0.31762186569466166
2212	22	-1.636114309165805
2212	23	-0.3353873062673145
2212	24	0.3172911395678
2212	25	-0.39497834838325474
2213	1	0.1685720970260789
2213	2	0.41588186019667006
2213	3	0.14702990220384748
2213	4	0.3716902043814372
2213	5	0.31437077999704854
2213	6	0.2623066177366198
2213	7	0.1540780037801568
2213	8	0.2050140287636495
2213	9	0.6767268534898747
2213	10	0.7016259833302314
2213	11	0.040532440659283964
2213	12	0.09732913856637282
2213	13	0.05202129361988739
2213	14	-1.8037046238989447
2213	15	-0.4615255605954758
2213	16	-0.08552206683432217
2213	17	-2.2032183550786755
2213	18	-0.9100163796615988
2213	19	-0.4584915954957012
2213	20	0.2072759406263387
2213	21	-0.2086892637360691
2213	22	0.3548474521832361
2213	23	0.8905963965720218
2213	24	0.663552308295273
2213	25	-0.8116305714812526
2214	1	0.33599648046428143
2214	2	0.26550079830303375
2214	3	0.19707258481397005
2214	4	0.6421557268973501
2214	5	0.4995769997542112
2214	6	0.4489370574085072
2214	7	0.38102843752742643
2214	8	0.23985906790118605
2214	9	0.677130095429817
2214	10	0.716590170942851
2214	11	0.024656945149594378
2214	12	-0.05866594777675971
2214	13	0.09576355079560273
2214	14	0.9565897382439275
2214	15	0.5590778509814385
2214	16	0.5175858733240304
2214	17	0.5397009167597275
2214	18	0.37792406200587436
2214	19	-0.6584356297093876
2214	20	0.34138835481689256
2214	21	0.6182852760559285
2214	22	0.7849239926029838
2214	23	1.2458371873795144
2214	24	0.8622421571901384
2214	25	0.9944733275596533
2215	1	-0.30766255790498676
2215	2	0.121788120222309
2215	3	-0.035343715427174516
2215	4	0.3170649599896791
2215	5	0.27222516063388014
2215	6	0.2328524655584232
2215	7	0.07241788779567315
2215	8	-0.009195616564383613
2215	9	0.117797149119383
2215	10	0.46580987670852636
2215	11	0.16092424434598485
2215	12	0.03925903140086007
2215	13	-0.10586459359232947
2215	14	0.7213019219877168
2215	15	0.2715563795190121
2215	16	0.24827625223952765
2215	17	0.40060156542117653
2215	18	0.3310265455297099
2215	19	-0.3453252637962052
2215	20	-1.759855795702471
2215	21	-1.995009583463073
2215	22	-0.3817095624896183
2215	23	0.2646389707311627
2215	24	0.48947290453203607
2215	25	-0.2558223918563627
2216	1	-0.18356039981951255
2216	2	-0.3754117790328344
2216	3	-0.2664364473385774
2216	4	0.18896472351910631
2216	5	0.21165126474623008
2216	6	-0.05714942340767948
2216	7	-0.06907138707454408
2216	8	-0.19276242766310503
2216	9	0.17075711236616087
2216	10	-1.9595494932629762
2216	11	-0.7497952884963723
2216	12	-0.346477758662715
2216	13	-0.24655700461269703
2216	14	-0.891734392802654
2216	15	-0.8006908604916205
2216	16	-0.3084821653199299
2216	17	0.11275391403570012
2216	18	-0.042973118654096254
2216	19	-1.5648432975923683
2216	20	-1.1412534440562752
2216	21	-1.0759212311545667
2216	22	-1.0285495087590277
2216	23	-4.548945144229915
2216	24	-2.1643814025438886
2216	25	-1.5023717638334375
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	9.0
4	42.0
5	72.0
6	79.0
7	285.0
8	157.0
9	423.0
10	1170.0
11	2455.0
12	4850.0
13	8043.0
14	11283.0
15	14703.0
16	17487.0
17	19474.0
18	20681.0
19	21450.0
20	22409.0
21	23356.0
22	25028.0
23	26716.0
24	29500.0
25	33038.0
26	37918.0
27	44592.0
28	51956.0
29	64831.0
30	79795.0
31	99612.0
32	131838.0
33	177379.0
34	240460.0
35	332096.0
36	468173.0
37	689622.0
38	1076137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.17009057191923	20.98989190777621	13.565755594931195	23.27426192537336
2	15.856891476963531	21.28980919128405	40.253317074010376	22.599982257742045
3	18.06411732737826	26.07361881950131	30.493867835081474	25.368396018038958
4	12.981190844789706	17.415717689187417	38.61744829568104	30.98564317034184
5	13.444756449890722	37.49724188186169	35.56606914724682	13.491932521000768
6	30.687119238380113	38.41778991372117	17.830904305785523	13.064186542113193
7	25.89913997896885	32.50255607137456	24.119012695750314	17.47929125390627
8	23.341417863014627	37.2417329107273	21.64689761690876	17.76995160934931
9	24.5106077796387	17.234477111200803	22.32080336703119	35.93411174212931
10	14.835193935907986	29.861320757714903	34.2893483283177	21.0141369780594
11	31.7148254779337	25.765546615483697	24.02697274761033	18.492655158972273
12	20.714290442807116	27.457404060665034	31.746615560807367	20.081689935720483
13	26.170785110047607	24.285177840910293	27.093939278382624	22.45009777065948
14	21.047964756738548	23.46651609427795	28.179566048539467	27.305953100444036
15	22.511864093462496	31.01627192960386	24.444548025643762	22.02731595128988
16	21.94190450743082	31.125691198446283	25.91639653400441	21.016007760118487
17	20.30802711490255	30.189850925235937	28.479651376797893	21.02247058306362
18	20.38275157745268	29.99558019301501	29.293998728345528	20.32766950118678
19	21.07928574038738	28.965889056418714	29.43008260155988	20.524742601634024
20	22.107414655820467	28.73323110628956	28.763973360949286	20.39538087694069
21	22.48010618858854	29.003830616173953	27.810268127245198	20.705795067992312
22	21.581811215255684	28.553036543808908	29.10696492316524	20.75818731777017
23	21.919923356226196	28.78275049041	28.41886203697035	20.878464116393463
24	21.47643738105622	28.229809380137898	28.786334536308917	21.50741870249696
25	22.134588070158326	28.495320313309612	28.174594865795445	21.195496750736613
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2322.0
1	2322.0
2	2676.0
3	3030.0
4	3030.0
5	3030.0
6	4483.0
7	5936.0
8	5936.0
9	5936.0
10	9741.0
11	13546.0
12	13546.0
13	13546.0
14	23523.5
15	33501.0
16	33501.0
17	33501.0
18	54355.0
19	75209.0
20	75209.0
21	75209.0
22	108516.0
23	141823.0
24	141823.0
25	141823.0
26	186523.0
27	231223.0
28	231223.0
29	231223.0
30	278214.0
31	325205.0
32	325205.0
33	325205.0
34	364073.5
35	402942.0
36	402942.0
37	402942.0
38	429124.0
39	455306.0
40	455306.0
41	455306.0
42	458473.5
43	461641.0
44	461641.0
45	461641.0
46	441569.0
47	421497.0
48	421497.0
49	421497.0
50	399123.5
51	376750.0
52	376750.0
53	376750.0
54	342084.5
55	307419.0
56	307419.0
57	307419.0
58	266310.0
59	225201.0
60	225201.0
61	225201.0
62	186453.5
63	147706.0
64	147706.0
65	147706.0
66	114366.5
67	81027.0
68	81027.0
69	81027.0
70	60076.0
71	39125.0
72	39125.0
73	39125.0
74	27981.5
75	16838.0
76	16838.0
77	16838.0
78	11795.0
79	6752.0
80	6752.0
81	6752.0
82	4486.5
83	2221.0
84	2221.0
85	2221.0
86	1446.5
87	672.0
88	672.0
89	672.0
90	425.5
91	179.0
92	179.0
93	179.0
94	110.5
95	42.0
96	42.0
97	42.0
98	25.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0851177166106893
2	0.0218949771810389
3	0.034258888116401855
4	0.041486613352706106
5	0.050382275182003645
6	0.017076493690169392
7	0.021683175708912764
8	0.03139956824269907
9	0.03214087339514053
10	0.02451602039859978
11	0.02292750935765379
12	0.017129444058200927
13	0.02483372260678898
14	0.034629540692622585
15	0.0326439018914401
16	0.023589388958047953
17	0.023721764878126786
18	0.024860197790804745
19	0.01800312513072122
20	0.014852578232845005
21	0.011781456887016093
22	0.016970592954106325
23	0.018267876970878887
24	0.017288295162295526
25	0.10889243185684763
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3777122.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.31460278505614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.71387809530167	70.44705277960989
2	9.3036083693123	14.944312212980822
3	1.66772567998255	4.0182817662670836
4	0.476203026143415	1.529842275590003
5	0.23674952607891203	0.9507222073289054
6	0.14190443975261063	0.683819922730011
7	0.0884643656837135	0.4973486273363581
8	0.06276561151262097	0.403279612575784
9	0.047914691115849034	0.3463424446084266
>10	0.23796441873849689	3.405789180270842
>50	0.01570510579531294	0.8910607069059342
>100	0.006190303278801657	0.9046277938140211
>500	5.624373193407994E-4	0.30012760881981604
>1k	3.639299843939127E-4	0.6773928611621242
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	4738	0.12543942186670168	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.64751840157665E-5	0.0	0.0	0.0
6	0.0	2.64751840157665E-5	0.0	0.0	0.0
7	0.0	2.64751840157665E-5	0.0	0.0	0.0
8	0.0	2.64751840157665E-5	0.0	0.0	0.0
9	0.0	2.64751840157665E-5	0.0	0.0	0.0
10	0.0	2.64751840157665E-5	0.0	0.0	0.0
11	0.0	2.64751840157665E-5	0.0	0.0	0.0
12	0.0	2.64751840157665E-5	0.0	0.0	0.0
13	0.0	2.64751840157665E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	100	1.9263916E-6	11.402655	5
GTAGGAC	235	0.0	9.704387	3
CAGGACC	515	0.0	9.409958	4
TCTGTCG	305	0.0	9.031061	8
GTCTTAT	465	0.0	8.99742	1
ACTGTCC	445	0.0	8.96458	8
CGCAGAT	160	6.7665496E-7	8.90455	8
CTTAGAC	225	2.6193447E-10	8.8687315	3
GACTGTC	315	0.0	8.744362	7
GGTATCA	3265	0.0	8.678634	1
GTCCTAA	395	0.0	8.6661	1
TACGACA	100	0.0029002745	8.5519905	4
GACTGCG	100	0.002911423	8.548367	7
AGAGCGC	100	0.002911423	8.548367	8
CTGTCGC	335	0.0	8.505837	9
GTCCAAT	350	0.0	8.421935	1
TAGGACA	410	0.0	8.343407	4
AATACGA	115	0.0011086095	8.262793	5
TCTATAC	490	0.0	8.144754	3
TTAGACA	455	0.0	8.144753	4
>>END_MODULE