FastQCFastQC Report
Wed 25 May 2016
SRR1295301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295301_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3777122
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT114880.3041469139731256No Hit
GGTATCAACGCAGAGTACTTTTTTT97680.2586095974660072No Hit
TATCAACGCAGAGTACTTTTTTTTT71010.18800028169595792No Hit
GTGGTATCAACGCAGAGTACATGGG54710.14484573175025853No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45180.11961488138323305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG551.9506212E-413.8270965
AGCGTCA3550.011.78243
ACCGCGT600.005893560611.0794738
TGGACCG700.001484251510.8641475
TACACCG700.001484251510.8641475
GCAGCGT3450.010.7604591
GGTATCA43400.010.0891231
CACCGAT856.618761E-410.055327
CAGCGTC4350.010.052592
GTCCTAG4150.09.8629241
GCGTCAG4250.09.8417694
CTAGGAC4200.09.5062553
CGTCAGA4450.09.3993195
GTCTTAG3750.09.391951
TAGACAG5600.08.9968725
GTCTAGA3500.08.9749331
AGACCGT1401.0906344E-58.8184726
ACACCGA1107.172168E-48.6334696
CAGGACT5650.08.5808674
GTATTAG5450.08.383591
Produced by FastQC (version 0.11.5)