FastQCFastQC Report
Wed 25 May 2016
SRR1295300_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295300_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438021
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA466761.9145036076391466No Hit
GCTTATGTACTCTGCGTTGATACCA279571.146708744510404No Hit
CTTATGTACTCTGCGTTGATACCAC182330.7478606623979039No Hit
CCCATGTACTCTGCGTTGATACCAC143500.5885921409208534No Hit
GAGTACATAAGCAGTGGTATCAACG123880.5081170342667269No Hit
GTATCAACGCAGAGTACTTTTTTTT120990.4962631577004464No Hit
TATGTACTCTGCGTTGATACCACTG118710.48691131044400354No Hit
CATGTACTCTGCGTTGATACCACTG96400.3954026647022319No Hit
GCGTTGATACCACTGCTTATGTACT93710.3843691256145866No Hit
GTGGTATCAACGCAGAGTACATGGG88220.3618508618260466No Hit
GGTATCAACGCAGAGTACTTTTTTT85720.3515966433431049No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA82820.33970174990289254No Hit
ACTCTGCGTTGATACCACTGCTTAT78210.32079297102034804No Hit
CATAAGCAGTGGTATCAACGCAGAG77460.31771670547546554No Hit
TATCAACGCAGAGTACTTTTTTTTT74370.3050424914305496No Hit
GTACATGGGAAGCAGTGGTATCAAC73630.30200724275959884No Hit
GTACATGATAAGCAGTGGTATCAAC69590.2854364256911651No Hit
CTGCTTATGTACTCTGCGTTGATAC68130.27944796209712713No Hit
ACGCAGAGTACATAAGCAGTGGTAT65730.2696039123535031No Hit
ATCATGTACTCTGCGTTGATACCAC65300.26784018677443716No Hit
GTATCAACGCAGAGTACATAAGCAG59520.24413243364187595No Hit
TATCAACGCAGAGTACATAAGCAGT52700.21615892562041097No Hit
GGTATCAACGCAGAGTACATAAGCA49080.20131081725711142No Hit
AAGCAGTGGTATCAACGCAGAGTAC46550.1909335481523744No Hit
GTACTGGTTCACTATCGGTCAGTCA43440.17817730035959495No Hit
GTGGTATCAACGCAGAGTACATAAG36260.1487271848765864No Hit
GCAGTGGTATCAACGCAGAGTACAT35920.1473326111629063No Hit
GTACTTTTTTTTTTTTTTTTTTTTT35880.14716854366717924No Hit
GTACTCTGCGTTGATACCACTGCTT34640.14208245129964017No Hit
GTACATGGGATAAGCAGTGGTATCA34040.13962143886373415No Hit
ACGCAGAGTACTTTTTTTTTTTTTT33810.13867805076330353No Hit
ATACAGGGTGACAGCCCCGTACACA32490.13326382340431028No Hit
GGTATCAACGCAGAGTACATGGGAA31950.13104891221199488No Hit
GGATACCACGTGTCCCGCCCTACTC31770.13031060848122308No Hit
GAGTACATGGGAAGCAGTGGTATCA31490.1291621360111336No Hit
GCTTATCCCATGTACTCTGCGTTGA31310.1284238322803618No Hit
ACATAAGCAGTGGTATCAACGCAGA29340.12034350811580376No Hit
GTATCAACGCAGAGTACATGGGAAG27530.11291945393415398No Hit
GAGTACATGATAAGCAGTGGTATCA26000.10664387222259364No Hit
GGTATCAACGCAGAGTACATGGGGG25770.10570048412216301No Hit
CCACTGCTTATGTACTCTGCGTTGA25710.10545438287857241No Hit
GATACCACTGCTTATGTACTCTGCG24970.10241913420762168No Hit
GCGTTGATACCACTGCTTCCCATGT24680.10122964486360045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCGA551.9637115E-413.81582410
CCCGACT853.954352E-612.29176812
GCTCTAT701.0847843E-412.2222331
ACCTTAG701.0847843E-412.2222331
CCGCTCT701.09313085E-412.2122019
TCCACGG658.0074044E-411.6955922
CCCCGTA7350.011.62923615
CGACTAA3650.011.44892617
GCGACTA3850.011.3468516
CCGTACA7500.011.27025417
CCGAACA1104.989597E-711.22420617
CCCGTAC7550.011.19492716
AGGGTGA6750.011.1220995
TAATACG600.00586321511.0869024
CGCTTAA951.3635383E-510.99812414
TTAGACC700.001487927610.8606394
CGACTCG909.535726E-510.55375514
GTGACAG7050.010.5094918
TCTTCGT1659.331416E-1010.36165511
GTACAGA2400.010.2983641
Produced by FastQC (version 0.11.5)