FastQCFastQC Report
Wed 25 May 2016
SRR1295300_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295300_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438021
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA503492.065158585590526No Hit
GTATCAACGCAGAGTACTTTTTTTT368351.5108565512766297No Hit
GCTTATGTACTCTGCGTTGATACCA310671.274271222438199No Hit
GGTATCAACGCAGAGTACTTTTTTT257931.0579482293220608No Hit
TATCAACGCAGAGTACTTTTTTTTT236300.9692287310076493No Hit
CTTATGTACTCTGCGTTGATACCAC213260.8747258534688586No Hit
CCCATGTACTCTGCGTTGATACCAC169620.6957282156306283No Hit
GAGTACATAAGCAGTGGTATCAACG137860.5654586240233369No Hit
GTACTTTTTTTTTTTTTTTTTTTTT133690.5483545875937902No Hit
ACGCAGAGTACTTTTTTTTTTTTTT119720.491054014711112No Hit
TATGTACTCTGCGTTGATACCACTG114200.46841270030077675No Hit
CATGTACTCTGCGTTGATACCACTG112560.461685932975967No Hit
GCGTTGATACCACTGCTTATGTACT105770.4338354755762973No Hit
CATAAGCAGTGGTATCAACGCAGAG98110.402416550144564No Hit
GTGGTATCAACGCAGAGTACATGGG97310.3991352002300226No Hit
ACTCTGCGTTGATACCACTGCTTAT88370.3624661149350231No Hit
CTGCTTATGTACTCTGCGTTGATAC80420.3298577001592685No Hit
ACGCAGAGTACATAAGCAGTGGTAT78690.3227617809690729No Hit
ATCATGTACTCTGCGTTGATACCAC75870.3111950225203146No Hit
GTACATGGGAAGCAGTGGTATCAAC73100.29983334844121523No Hit
GTACATGATAAGCAGTGGTATCAAC70370.2886357418578429No Hit
GTATCAACGCAGAGTACATAAGCAG64910.26624052869109827No Hit
GAGTACTTTTTTTTTTTTTTTTTTT64530.2646818874816911No Hit
TATCAACGCAGAGTACATAAGCAGT59870.24556802422948779No Hit
GGTATCAACGCAGAGTACATAAGCA53850.22087586612256413No Hit
AAGCAGTGGTATCAACGCAGAGTAC52660.2159948581246839No Hit
GTACTGGTTCACTATCGGTCAGTCA46920.19245117248784976No Hit
GCAGAGTACTTTTTTTTTTTTTTTT46040.18884168758185432No Hit
GCAGTGGTATCAACGCAGAGTACAT41910.1719017186480346No Hit
GTGGTATCAACGCAGAGTACATAAG40430.16583122130613312No Hit
ATACAGGGTGACAGCCCCGTACACA38780.1590634371073916No Hit
ACATAAGCAGTGGTATCAACGCAGA38570.1582020827548245No Hit
GGATACCACGTGTCCCGCCCTACTC37180.15250073727830893No Hit
GGTATCAACGCAGAGTACATGGGAA36230.14860413425479108No Hit
GTACTCTGCGTTGATACCACTGCTT36130.14819396551547342No Hit
GTACATGGGATAAGCAGTGGTATCA36050.14786583052401928No Hit
GAGTACATGGGAAGCAGTGGTATCA34520.14159024881245896No Hit
GCTTATCCCATGTACTCTGCGTTGA33700.13822686515005408No Hit
GTGGTATCAACGCAGAGTACTTTTT33370.13687330831030578No Hit
ATCAACGCAGAGTACTTTTTTTTTT33360.13683229143637402No Hit
GGTATCAACGCAGAGTACATGGGGG29780.12214825056880149No Hit
CCACTGCTTATGTACTCTGCGTTGA29660.12165604808162031No Hit
GTATCAACGCAGAGTACATGGGAAG29320.12026147436794021No Hit
GATACCACTGCTTATGTACTCTGCG28540.11706215820126242No Hit
GAGTACATGATAAGCAGTGGTATCA28320.11615978697476356No Hit
GCGTTGATACCACTGCTTCCCATGT27290.11193504895979156No Hit
GTTAATGATAGTGTGTCGAAACACA25900.10623370348327599No Hit
ATACCACTGCTTATGTACTCTGCGT25030.10266523545121227No Hit
GGTATCAACGCAGAGTACATGGGGA25020.1026242185772805No Hit
GGATAAGCAGTGGTATCAACGCAGA24720.10139371235932751No Hit
ACCCTGTATCGCGCGCCTTTCCAGA24570.100778459250351No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCC250.006032438519.00032819
GTCCAAT1501.8189894E-1212.6897961
ACGCAGC1001.4515172E-712.3448937
ATTAGTC701.0841297E-412.2230233
AGTCCCG550.003076934312.08690310
ACTGTCG550.003078825312.0859118
TGATCAA1052.73385E-711.75800710
CTCTCGG658.04864E-411.68843314
TATACGG907.4061227E-611.6194185
GGGTGTA752.082721E-411.3952877
GACTATC1001.940498E-611.3952877
TATACTG1604.5474735E-1111.289325
AAGGTAT1104.9920345E-711.2237826
ACGTAAC855.3479824E-511.1718516
GTTCTAT1451.1823431E-911.158271
TTAGGGA1451.204171E-911.1458984
AGGCTCG600.00584608211.0912625
TGACGTT600.00589180111.0796614
GTCATAT1303.18214E-810.9815551
AAATCGC2550.010.79945617
Produced by FastQC (version 0.11.5)