FastQCFastQC Report
Wed 25 May 2016
SRR1295299_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295299_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105004
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA868214.124505226593394No Hit
GCTTATGTACTCTGCGTTGATACCA511822.431444310794659No Hit
CTTATGTACTCTGCGTTGATACCAC342951.6292130561272093No Hit
GAGTACATAAGCAGTGGTATCAACG226801.0774326319569938No Hit
CCCATGTACTCTGCGTTGATACCAC223921.0637509477416671No Hit
TATGTACTCTGCGTTGATACCACTG216091.026553868781247No Hit
GCGTTGATACCACTGCTTATGTACT175810.8352003131585498No Hit
CATGTACTCTGCGTTGATACCACTG159900.7596185090384626No Hit
CATAAGCAGTGGTATCAACGCAGAG146760.6971958248060337No Hit
ACTCTGCGTTGATACCACTGCTTAT140060.6653669066662107No Hit
GTACATGATAAGCAGTGGTATCAAC131570.6250344417397782No Hit
CTGCTTATGTACTCTGCGTTGATAC128540.610640169804903No Hit
ATCATGTACTCTGCGTTGATACCAC127450.6054620323761856No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA122670.5827542370465805No Hit
ACGCAGAGTACATAAGCAGTGGTAT122600.5824216961107912No Hit
GTATCAACGCAGAGTACATAAGCAG108240.5142032984260363No Hit
GTACATGGGAAGCAGTGGTATCAAC106170.5043695878962701No Hit
TATCAACGCAGAGTACATAAGCAGT97160.4615668188754036No Hit
GGTATCAACGCAGAGTACATAAGCA91010.43235072237392425No Hit
GTATCAACGCAGAGTACTTTTTTTT84730.4025170498488364No Hit
AAGCAGTGGTATCAACGCAGAGTAC82760.39315839779876904No Hit
GTGGTATCAACGCAGAGTACATGGG74220.35258840363248717No Hit
GTGGTATCAACGCAGAGTACATAAG68840.327030257424689No Hit
GTACTGGTTCACTATCGGTCAGTCA67620.321234543972363No Hit
GCAGTGGTATCAACGCAGAGTACAT63820.30318232174380666No Hit
GTACTCTGCGTTGATACCACTGCTT59920.2846550410355515No Hit
GGTATCAACGCAGAGTACTTTTTTT59140.28094958489390043No Hit
GTACATGGGATAAGCAGTGGTATCA59000.28028450302232205No Hit
ACATAAGCAGTGGTATCAACGCAGA55580.2640375030166214No Hit
ATACAGGGTGACAGCCCCGTACACA52940.25149595915257167No Hit
GGATACCACGTGTCCCGCCCTACTC52310.24850309073046895No Hit
GCTTATCCCATGTACTCTGCGTTGA51810.24612779833197468No Hit
CCACTGCTTATGTACTCTGCGTTGA51620.24522518722054684No Hit
TATCAACGCAGAGTACTTTTTTTTT49720.2361990761062687No Hit
GAGTACATGATAAGCAGTGGTATCA49710.2361515702582988No Hit
GAGTACATGGGAAGCAGTGGTATCA46790.22227986265109237No Hit
GTTGATACCACTGCTTATGTACTCT45690.217054219374405No Hit
GATACCACTGCTTATGTACTCTGCG44930.2134437749286937No Hit
ATACCACTGCTTATGTACTCTGCGT43030.20441766381441556No Hit
ATCATTAACTGAATCCATAGGTTAA40370.1917811082544261No Hit
GCTTATCATGTACTCTGCGTTGATA39880.18945332170390175No Hit
GATAAGCAGTGGTATCAACGCAGAG39050.18551033632240127No Hit
ACCCTGTATCGCGCGCCTTTCCAGA37200.17672175444797253No Hit
GTACTTTTTTTTTTTTTTTTTTTTT37150.17648422520812312No Hit
GCGTTGATACCACTGCTTATCATGT35650.16935834801264035No Hit
GTATCAACGCAGAGTACATGGGAAG35310.16774314918166425No Hit
GCGTTGATACCACTGCTTCCCATGT35130.16688804391820633No Hit
GGATAAGCAGTGGTATCAACGCAGA34510.16394268134407344No Hit
TACCACTGCTTATGTACTCTGCGTT33760.16037974274633207No Hit
CAGTGGTATCAACGCAGAGTACATA33670.1599521901146031No Hit
TGATACCACTGCTTATGTACTCTGC33480.1590495790031753No Hit
GGTATCAACGCAGAGTACATGGGAA32420.1540139591183675No Hit
GAACAAAAAAAAAAAAAAAAAAAAA31870.1514011374800238No Hit
GGTTAATGAGGCGAACCGGGGGAAC30690.14579544741957737No Hit
GGGATAAGCAGTGGTATCAACGCAG30680.14574794157160748No Hit
CCCCATGTACTCTGCGTTGATACCA30170.14332514332514332No Hit
CCCATATTCAGACAGGATACCACGT30140.14318262578123367No Hit
ACGCAGAGTACATGATAAGCAGTGG29930.14218500297386608No Hit
CAGTGGTATCAACGCAGAGTACATG29310.13923964039973322No Hit
GCAGAGTACATAAGCAGTGGTATCA29090.13819451174439573No Hit
TATCAACGCAGAGTACATGGGAAGC29060.13805199420048608No Hit
GAGTACATGGGATAAGCAGTGGTAT28880.13719688893702814No Hit
GTATTTAGCCTTGGAGGATGGTCCC28850.1370543713931185No Hit
ACGCAGAGTACATGGGAAGCAGTGG28770.1366743246093594No Hit
GTTAATGATAGTGTGTCGAAACACA28210.13401399712304585No Hit
TATCAACGCAGAGTACATGATAAGC27790.1320187515083107No Hit
GTTCACTATCGGTCAGTCAGGAGTA26140.12418028659327963No Hit
TCTAAGTACCCCGAGGAAAAGAAAT26140.12418028659327963No Hit
ACTCTGCGTTGATACCACTGCTTCC26090.1239427573534302No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA25450.12090238308335756No Hit
GCCTTGGAGGATGGTCCCCCCATAT25040.11895464331659227No Hit
GTATCAACGCAGAGTACATGATAAG24910.11833706729298377No Hit
GGTATCAACGCAGAGTACATGGGAT24200.11496415208712193No Hit
GCCCAGAGCCTGAATCAGTGTGTGT24140.11467911699930261No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23930.11368149419193503No Hit
CTATCGGTCAGTCAGGAGTATTTAG23660.11239883629674814No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG23080.1096434971144948No Hit
GCGTTGATACCACTGCTTATCCCAT22900.10878839185103686No Hit
TATTCAGACAGGATACCACGTGTCC22800.10831333337133801No Hit
ATGATAAGCAGTGGTATCAACGCAG22760.10812330997945847No Hit
GTATCAACGCAGAGTACATGGGATA22620.10745822810788008No Hit
GGTATCAACGCAGAGTACATGATAA22550.10712568717209088No Hit
GCTTACATGTACTCTGCGTTGATAC21950.10427533629389776No Hit
GAGTACATGTAAGCAGTGGTATCAA21890.10399030120607847No Hit
CACACACACTGATTCAGGCTCTGGG21850.10380027781419893No Hit
TCCATGTACTCTGCGTTGATACCAC21740.1032777134865302No Hit
CATCTAAGTACCCCGAGGAAAAGAA21740.1032777134865302No Hit
GGTATCAACGCAGAGTACATGGGGG21730.1032302076385603No Hit
GTACACAAGCAGTGGTATCAACGCA21610.10266013746292169No Hit
GTCAGGAGTATTTAGCCTTGGAGGA21520.10223258483119271No Hit
ATCAACGCAGAGTACATAAGCAGTG21190.10066489184818651No Hit
CATGTAAGCAGTGGTATCAACGCAG21160.10052237430427685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGGT250.00603739418.9974813
GTACAAA5000.015.4004051
CCTTATT704.432859E-714.930142
CCCACGG456.776642E-414.77406216
TAGGGCC400.00528290614.24878810
CATAAGG1101.8553692E-1013.820624
TAATGGC551.957739E-413.820624
TCTATTA551.9589237E-413.8196342
CTACACA1205.0931703E-1113.4607084
ACCATAG500.001501354513.29823614
TCGTTGA2100.013.11730814
TTGTATC3050.013.08023313
GTCCTGA905.3638723E-712.6752291
ATCGGAA604.097676E-412.66498813
GAACAAA15900.012.4958641
CGCAGAA1850.012.325622
GACTAAG3700.012.32151918
GCTTACG853.915491E-612.3024291
CCGTGTT853.9489387E-612.2930729
ACTCTTC2250.012.24340259
Produced by FastQC (version 0.11.5)