FastQCFastQC Report
Wed 25 May 2016
SRR1295299_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295299_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105004
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA931534.425312255938707No Hit
GCTTATGTACTCTGCGTTGATACCA573732.7255530155762173No Hit
CTTATGTACTCTGCGTTGATACCAC398911.8950557813666862No Hit
GTATCAACGCAGAGTACTTTTTTTT276871.3152944127422086No Hit
CCCATGTACTCTGCGTTGATACCAC271421.2894037255986213No Hit
GAGTACATAAGCAGTGGTATCAACG254931.2110665822962807No Hit
TATGTACTCTGCGTTGATACCACTG213401.013774795677348No Hit
GCGTTGATACCACTGCTTATGTACT199270.9466490324959002No Hit
GGTATCAACGCAGAGTACTTTTTTT189920.9022310646440577No Hit
CATAAGCAGTGGTATCAACGCAGAG185470.8810909622974589No Hit
CATGTACTCTGCGTTGATACCACTG183490.8716848043994215No Hit
TATCAACGCAGAGTACTTTTTTTTT182660.8677418190179211No Hit
ACTCTGCGTTGATACCACTGCTTAT165350.78550919618205No Hit
GTACTTTTTTTTTTTTTTTTTTTTT154050.7318275879760799No Hit
ACGCAGAGTACATAAGCAGTGGTAT150540.7151530353386502No Hit
CTGCTTATGTACTCTGCGTTGATAC147800.7021364329949017No Hit
ATCATGTACTCTGCGTTGATACCAC146920.6979559183735518No Hit
GTACATGATAAGCAGTGGTATCAAC131100.6228016668851936No Hit
GTATCAACGCAGAGTACATAAGCAG122840.5835618364620685No Hit
TATCAACGCAGAGTACATAAGCAGT110580.5253196668509893No Hit
GTACATGGGAAGCAGTGGTATCAAC107300.5097377487168671No Hit
ACGCAGAGTACTTTTTTTTTTTTTT99220.4713530235571999No Hit
GGTATCAACGCAGAGTACATAAGCA99030.4704504124457721No Hit
AAGCAGTGGTATCAACGCAGAGTAC95180.4521606609773663No Hit
GTGGTATCAACGCAGAGTACATGGG78960.3751061755702127No Hit
GTGGTATCAACGCAGAGTACATAAG74480.3538235556797042No Hit
GCAGTGGTATCAACGCAGAGTACAT73500.34916798257865544No Hit
GTACTGGTTCACTATCGGTCAGTCA71500.33966681298467843No Hit
ACATAAGCAGTGGTATCAACGCAGA70650.3356288159072382No Hit
ATACAGGGTGACAGCCCCGTACACA62700.29786166677117953No Hit
GTACTCTGCGTTGATACCACTGCTT60500.2874103802178048No Hit
GTACATGGGATAAGCAGTGGTATCA59900.2845600293396117No Hit
GGATACCACGTGTCCCGCCCTACTC59060.28056953811014135No Hit
CCACTGCTTATGTACTCTGCGTTGA58170.27634151764082154No Hit
GCTTATCCCATGTACTCTGCGTTGA57960.275343894833454No Hit
GAGTACTTTTTTTTTTTTTTTTTTT56080.26641279541511564No Hit
GAGTACATGATAAGCAGTGGTATCA53940.2562465439495602No Hit
GATACCACTGCTTATGTACTCTGCG51960.24684038605152295No Hit
GAGTACATGGGAAGCAGTGGTATCA49800.23657912289002775No Hit
ATACCACTGCTTATGTACTCTGCGT48190.22893068136687628No Hit
ATCATTAACTGAATCCATAGGTTAA47060.22356252054627923No Hit
GCTTATCATGTACTCTGCGTTGATA46970.2231349679145503No Hit
GTTGATACCACTGCTTATGTACTCT46710.22189981586733326No Hit
GATAAGCAGTGGTATCAACGCAGAG44810.21287370475305512No Hit
GGATAAGCAGTGGTATCAACGCAGA43050.20451267551035535No Hit
ACCCTGTATCGCGCGCCTTTCCAGA42900.20380008779080702No Hit
CAGTGGTATCAACGCAGAGTACATA42520.20199486556795143No Hit
GCGTTGATACCACTGCTTCCCATGT39950.18978586263969094No Hit
GCGTTGATACCACTGCTTATCATGT39420.18726805269728702No Hit
GCAGAGTACTTTTTTTTTTTTTTTT39040.1854628304744314No Hit
TGATACCACTGCTTATGTACTCTGC38180.18137732754902128No Hit
TACCACTGCTTATGTACTCTGCGTT37440.1778618947992498No Hit
CCCCATGTACTCTGCGTTGATACCA37360.1774818480154907No Hit
CCCATATTCAGACAGGATACCACGT36880.1752015673129362No Hit
GGGATAAGCAGTGGTATCAACGCAG36760.17463149713729761No Hit
GTATCAACGCAGAGTACATGGGAAG36710.17439396789744818No Hit
ACGCAGAGTACATGATAAGCAGTGG35750.16983340649233922No Hit
GCAGAGTACATAAGCAGTGGTATCA35320.16779065502963417No Hit
GGTATCAACGCAGAGTACATGGGAA35000.16627046789459782No Hit
CAGTGGTATCAACGCAGAGTACATG34600.16437023397580242No Hit
ACGCAGAGTACATGGGAAGCAGTGG34070.1618524240333985No Hit
TATCAACGCAGAGTACATGGGAAGC33490.15909708485114518No Hit
GGTTAATGAGGCGAACCGGGGGAAC32860.1561042164290424No Hit
GAGTACATGGGATAAGCAGTGGTAT32730.15548664040543392No Hit
ACTCTGCGTTGATACCACTGCTTCC31200.1482182456660415No Hit
GTATTTAGCCTTGGAGGATGGTCCC30560.14517787139596883No Hit
TATCAACGCAGAGTACATGATAAGC30130.14313511993326378No Hit
GTTAATGATAGTGTGTCGAAACACA29430.13980971057537184No Hit
GCCTTGGAGGATGGTCCCCCCATAT28750.13657931291341965No Hit
CTATCGGTCAGTCAGGAGTATTTAG28730.13648430121747987No Hit
TCTAAGTACCCCGAGGAAAAGAAAT28560.13567670180199182No Hit
ATCAACGCAGAGTACTTTTTTTTTT28460.13520164332229298No Hit
ATGATAAGCAGTGGTATCAACGCAG28390.13486910238650376No Hit
GCCCAGAGCCTGAATCAGTGTGTGT28040.13320639770755782No Hit
GTATCAACGCAGAGTACATGATAAG27240.129405929869967No Hit
CACACACACTGATTCAGGCTCTGGG26360.1252254152486171No Hit
TATTCAGACAGGATACCACGTGTCC26290.1248928743128279No Hit
GTGGTATCAACGCAGAGTACTTTTT26280.12484536846485803No Hit
GGTATCAACGCAGAGTACATGGGAT26100.1239902632014001No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG26010.12356271056967114No Hit
CATGTAAGCAGTGGTATCAACGCAG26000.12351520472170124No Hit
ATCAACGCAGAGTACATAAGCAGTG25880.12294513454606261No Hit
GTTCACTATCGGTCAGTCAGGAGTA25640.12180499419478538No Hit
GCGTTGATACCACTGCTTATCCCAT25490.1210924064752371No Hit
AAAAAGTACTCTGCGTTGATACCAC25170.11957221934020076No Hit
GTATCAACGCAGAGTACATGGGATA24990.11871711407674285No Hit
ACTTAGATGTTTCAGTTCCCCCGGT24870.11814704390110424No Hit
GAGTACATGTAAGCAGTGGTATCAA24810.11786200881328492No Hit
GCTTACATGTACTCTGCGTTGATAC24800.11781450296531504No Hit
TCCATGTACTCTGCGTTGATACCAC24780.11771949126937525No Hit
GGTATCAACGCAGAGTACATGATAA24630.11700690354982698No Hit
GTCAGGAGTATTTAGCCTTGGAGGA24180.11486914039118216No Hit
GGTATCAACGCAGAGTACATGGGGG23890.11349147080005548No Hit
CTGCTTATCATGTACTCTGCGTTGA23720.11268387138456744No Hit
CATCTAAGTACCCCGAGGAAAAGAA23310.11073613161780216No Hit
GTACACAAGCAGTGGTATCAACGCA22810.1083608392193079No Hit
ACGCAGAGTACATGGGATAAGCAGT22740.1080282982835187No Hit
TACATGTACTCTGCGTTGATACCAC21360.10147249126367455No Hit
GCTTCCCATGTACTCTGCGTTGATA21350.10142498541570467No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGAC250.00601336519.0106034
CTGGACA359.8992874E-519.0106034
CGCTGTT250.006043151518.9943477
GTTCGTA359.964494E-518.99434712
CGCTAAT402.7276092E-416.652091
GGACTCG402.766974E-416.6208426
TTCGTAT402.7679768E-416.62005213
TAGACTT350.00216246916.2948045
TCGTGCA350.00216246916.2948042
GACAGTC350.002174861716.2808697
GTCTTGT601.4427151E-615.8591361
CCGATTC508.7384324E-515.195477515
CTAGTAC500.001493563213.3074224
GTCGCAA500.001503222713.29604218
AGAAGTC500.001503222713.29604217
ACACCGC655.4670905E-513.1505576
CCGAACA655.469528E-513.14993317
ACAACTT604.073879E-412.6737355
AGCCGAT604.1033854E-412.66289813
AACTTGT604.1033854E-412.6628987
Produced by FastQC (version 0.11.5)