FastQCFastQC Report
Wed 25 May 2016
SRR1295298_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295298_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4040225
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG49990.123730732818098No Hit
GTATCAACGCAGAGTACTTTTTTTT44200.10939984778075477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAC1354.7257345E-911.2608813
GTGGTAT14100.010.5863561
GGTATCA27600.010.2308871
TGGTATC13850.09.7413552
TAATACG800.0045127229.50136854
CTTGCAC4150.09.3868933
GTATTAG6250.09.2793121
TACACTG7600.09.0012965
ACCGCCC950.00182711178.99873358
GTATACG850.00739111058.9482271
CTTACGT850.00743027038.9424653
AGTGCGC850.0074476488.9399188
TCAGGAC4050.08.9148643
CGTAGCA1401.0842887E-58.822592
GTATAAA8200.08.8118211
GTCTTGC7150.08.7761461
ATAGGAC3700.08.4741943
GTCCTAG4050.08.4511031
TAGGACA5400.08.4456624
GTATCAA49450.08.4404221
Produced by FastQC (version 0.11.5)