FastQCFastQC Report
Wed 25 May 2016
SRR1295298_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295298_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4040225
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT128700.3185466155969036No Hit
GGTATCAACGCAGAGTACTTTTTTT105930.26218836822206687No Hit
TATCAACGCAGAGTACTTTTTTTTT77860.19271203955225266No Hit
GTGGTATCAACGCAGAGTACATGGG59770.14793730547184872No Hit
ACGCAGAGTACTTTTTTTTTTTTTT50520.12504254094759573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA500.001504179813.29540812
AGCGTCA3300.011.5221563
GGTATCA43750.011.5100981
CTAGTAC2700.010.9140423
GTGGTAT18450.010.6285081
CTAGGAC4500.010.5619763
TCTAGAC3850.010.369943
TGGTATC18300.010.2329972
TAGACGT900.0011081459.5057784
CAGCGTC4100.09.273932
GCAGCGT3850.09.1483431
TAGGACC2201.6552804E-109.0736984
TATACCG1054.469271E-49.0528985
CTACACT5500.08.9872814
GTACTAG2651.8189894E-128.9803971
GACCGGG850.007460158.938097
CTGCGAA850.007460158.9380918
TTAGACA5250.08.872064
TTTAGAC3750.08.6185733
TAGACAG5750.08.5963175
Produced by FastQC (version 0.11.5)