FastQCFastQC Report
Wed 25 May 2016
SRR1295297_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295297_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4285551
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG48890.11408101315326781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG700.001488737910.8602795
TGGTATC15050.010.1025862
GTGGTAT16150.09.8913291
ACCCGTG1356.468963E-79.8498687
GGTATCA30400.09.6650211
GTCCTAG3600.09.5086291
CCTACAC5400.09.5027453
CGTAGAC1401.0556978E-69.5027453
TCGCGTT800.0045261359.49797510
CTACACT5850.09.4215234
CTAGTAC2950.09.3416813
TATACTG7750.09.0735885
GTCCTAA3900.09.0210071
GTCCAAG6300.08.9049061
GTCTTAG3300.08.6442091
CCGCACT1000.00289896958.5524694
CCGATCC1000.00291217198.5481789
CTGTCGC3150.08.4426449
GTTAGAC2606.002665E-118.4062743
GGACCGT1252.7511982E-48.3583156
Produced by FastQC (version 0.11.5)