##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295297_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4285551 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.80739326168327 31.0 30.0 33.0 25.0 34.0 2 29.80272128368091 31.0 30.0 34.0 25.0 34.0 3 30.064349718390936 31.0 30.0 34.0 25.0 34.0 4 33.230051864976055 35.0 33.0 37.0 26.0 37.0 5 33.66857097255406 35.0 35.0 37.0 28.0 37.0 6 33.85401527131517 35.0 35.0 37.0 28.0 37.0 7 33.75368558208734 35.0 35.0 37.0 28.0 37.0 8 33.68292455275879 35.0 35.0 37.0 28.0 37.0 9 34.59857763914138 38.0 34.0 39.0 26.0 39.0 10 34.50967378523789 37.0 34.0 39.0 26.0 39.0 11 34.970588846101705 38.0 34.0 39.0 27.0 39.0 12 34.949539510788696 38.0 34.0 39.0 27.0 39.0 13 34.866981632000176 38.0 34.0 39.0 27.0 39.0 14 34.96171064117543 38.0 33.0 40.0 23.0 41.0 15 35.60291803784391 39.0 34.0 41.0 27.0 41.0 16 35.68288628463411 39.0 34.0 41.0 27.0 41.0 17 35.47283651507122 39.0 34.0 40.0 25.0 41.0 18 35.435566395079654 38.0 34.0 40.0 25.0 41.0 19 35.50208269601738 39.0 34.0 40.0 25.0 41.0 20 35.47979291344333 39.0 34.0 40.0 25.0 41.0 21 35.47107011443803 39.0 34.0 40.0 25.0 41.0 22 35.19463074876486 39.0 34.0 40.0 24.0 41.0 23 34.86950569483364 38.0 33.0 40.0 23.0 41.0 24 35.20075948226961 39.0 33.0 40.0 25.0 41.0 25 33.700485421827906 38.0 31.0 40.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29743529824320447 1101 2 0.649060580325461 1101 3 0.42264428015748123 1101 4 0.31307036122057497 1101 5 0.18788297455945013 1101 6 0.18405569395000754 1101 7 0.23739995480752185 1101 8 0.2219768117269112 1101 9 0.04753664323307305 1101 10 0.39253237537552366 1101 11 0.36472198088795693 1101 12 0.4329348933650152 1101 13 0.39739643077192 1101 14 1.0306580630555189 1101 15 0.660821808569743 1101 16 0.4453491875188078 1101 17 0.7915393438255549 1101 18 0.7321297935215583 1101 19 0.8020213925600217 1101 20 0.8723268552322523 1101 21 0.8820577650038715 1101 22 0.7063096304673024 1101 23 -1.3525565197613503 1101 24 0.1463205527475182 1101 25 0.5676194522022584 1102 1 0.2799023215072971 1102 2 0.3774629364688806 1102 3 0.2927936226476717 1102 4 0.28350308462950125 1102 5 0.07637276421386474 1102 6 0.07105875156511132 1102 7 0.0068918834070430535 1102 8 0.0627224477780004 1102 9 0.2449595074585531 1102 10 0.8020370715696501 1102 11 0.47357947300412917 1102 12 0.34485260099562964 1102 13 0.382324082845372 1102 14 0.9781054926445805 1102 15 0.6345058351152559 1102 16 0.4847491318870496 1102 17 0.7898964378869167 1102 18 0.7288937731317802 1102 19 0.771318972365485 1102 20 0.8303782090981855 1102 21 0.7650712583690904 1102 22 0.7466179923891758 1102 23 0.7228477643101741 1102 24 0.8439358900603366 1102 25 0.6361948422906423 1103 1 -0.5489897054268553 1103 2 -1.6520508212077303 1103 3 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22 0.3733586033619929 2208 23 0.38110601035740643 2208 24 0.7045836278554702 2208 25 1.031800974562394 2209 1 0.2837006205764716 2209 2 -0.6987562592720984 2209 3 0.08053139302201373 2209 4 0.3021683729171869 2209 5 0.4655051806740502 2209 6 0.41933521978984345 2209 7 0.40777026517896076 2209 8 0.365277568986059 2209 9 0.9940705785762134 2209 10 0.8245292867976701 2209 11 0.5330957289473446 2209 12 0.5017149612045628 2209 13 0.4555688678852192 2209 14 1.255269784816285 2209 15 0.7368566269856061 2209 16 0.7456619173286896 2209 17 0.732157746733094 2209 18 0.11140503175361971 2209 19 0.8121173718833319 2209 20 0.8989574882115576 2209 21 0.892033188322884 2209 22 1.0572642540244814 2209 23 1.4959178105165591 2209 24 1.2412836684705653 2209 25 1.217585135600764 2210 1 0.5618046313483269 2210 2 0.7677811795977583 2210 3 0.5461012338424815 2210 4 0.7262391071528427 2210 5 0.7143953695581118 2210 6 0.6205051824081238 2210 7 0.5235662842371624 2210 8 0.5827884282172562 2210 9 1.023855565137822 2210 10 0.9732718078108533 2210 11 0.671554643080384 2210 12 0.5474116243673777 2210 13 0.6001783939143408 2210 14 -1.141112077748744 2210 15 -0.03078921077983665 2210 16 0.48634359609035016 2210 17 0.903037668197129 2210 18 0.7582462375684642 2210 19 1.0573449755551678 2210 20 1.1550960838467859 2210 21 1.2528468155389945 2210 22 1.350923520931815 2210 23 1.7194323198831327 2210 24 1.4809764213521603 2210 25 1.2744743676435988 2211 1 0.20631284505619618 2211 2 -0.5838361399217753 2211 3 -0.0629614990263363 2211 4 0.39787012117268006 2211 5 0.3239889071045994 2211 6 0.21331789640437648 2211 7 0.22853526739930174 2211 8 0.32404359544162276 2211 9 0.7962560191545194 2211 10 0.8316738959029664 2211 11 0.44239193422775713 2211 12 0.25774360785645456 2211 13 0.25153612563001815 2211 14 -0.8542888786487168 2211 15 0.01675646644518025 2211 16 0.17167900078965204 2211 17 0.31223637592984943 2211 18 0.2915892778924274 2211 19 0.4948168753817157 2211 20 0.6095360314701921 2211 21 0.627126185402993 2211 22 -0.14350480434121238 2211 23 0.4350500495489129 2211 24 -0.25991204589253414 2211 25 0.29285844054665233 2212 1 0.0965305110012622 2212 2 -1.0781496745716161 2212 3 -0.21831495980289617 2212 4 0.19821878069322452 2212 5 0.25848886071002397 2212 6 0.22247807702540712 2212 7 0.1978379799135368 2212 8 0.22589158453527602 2212 9 -2.0856849739979566 2212 10 0.18296626482941747 2212 11 0.1756203567641066 2212 12 0.05833953324736996 2212 13 0.1720423035540719 2212 14 0.7713959878983516 2212 15 0.3443453626917048 2212 16 0.5010453486368576 2212 17 0.5601262373712501 2212 18 0.3373522114865537 2212 19 -0.4094013310462472 2212 20 0.5025985027004509 2212 21 0.5066630134671684 2212 22 -1.5294095071384248 2212 23 -0.24217936658828165 2212 24 0.31074456327421274 2212 25 -0.18862430742544944 2213 1 0.27402753398604673 2213 2 0.5274033590932845 2213 3 0.30537207313595616 2213 4 0.5209471735539424 2213 5 0.5285870844485032 2213 6 0.3835962478154826 2213 7 0.33887456013936657 2213 8 0.41305978904387075 2213 9 0.9231234827276893 2213 10 0.8672812138220962 2213 11 0.2514425200327608 2213 12 0.2427481447745805 2213 13 0.38212135311835027 2213 14 -1.4945462838444357 2213 15 -0.3025118797799209 2213 16 0.09210281318559055 2213 17 -1.919843504785483 2213 18 -0.6420870454530316 2213 19 -0.3420341089930474 2213 20 0.4743006085249064 2213 21 0.059447362425352424 2213 22 0.5116538638020316 2213 23 1.0945861738025542 2213 24 0.8823000953064764 2213 25 -0.6337381227461876 2214 1 0.5324742692742959 2214 2 0.4479522181335298 2214 3 0.3777764983684655 2214 4 0.808813769484857 2214 5 0.7159408091402213 2214 6 0.6660345417403093 2214 7 0.5590003754281696 2214 8 0.5623834154761198 2214 9 0.9861081238820688 2214 10 0.9785704815490064 2214 11 0.3721647853945669 2214 12 0.2707322351582775 2214 13 0.41187371377583304 2214 14 1.3855190158242152 2214 15 0.9098898706335277 2214 16 0.8800423924066649 2214 17 0.6967278513848285 2214 18 0.6640487128520007 2214 19 -0.48244025174095384 2214 20 0.5669394348449259 2214 21 0.8387812732034661 2214 22 0.9905201381619051 2214 23 1.5885404158729415 2214 24 1.317511247943557 2214 25 1.4560197777258495 2215 1 0.19950148755166097 2215 2 0.5560280752240772 2215 3 0.4818615088809288 2215 4 0.8913593610952901 2215 5 0.6606535206444306 2215 6 0.6949175661567395 2215 7 0.5029712991201123 2215 8 0.48806986454430046 2215 9 0.6667763393767814 2215 10 1.1070342442355923 2215 11 0.7994832486821508 2215 12 0.6310299158484085 2215 13 0.5283654698361886 2215 14 1.4365915523768678 2215 15 0.9304071935305629 2215 16 0.8944003985758755 2215 17 1.0723764199301087 2215 18 0.9307065043364773 2215 19 0.2218150292056933 2215 20 -1.208897389066422 2215 21 -1.483496124987191 2215 22 0.325537346462049 2215 23 0.9911536516398272 2215 24 1.1798021981663709 2215 25 0.5167588846445383 2216 1 0.13003709959670928 2216 2 -0.15374971792200753 2216 3 0.03203651936038909 2216 4 0.5732479876252796 2216 5 0.4711839529438322 2216 6 0.2546025849303817 2216 7 0.29959296174789785 2216 8 0.17827111248193006 2216 9 0.6418892846478954 2216 10 -1.4539979788632493 2216 11 -0.19327882127781493 2216 12 0.05745809780474076 2216 13 0.23789109734519798 2216 14 -0.2887372391522476 2216 15 -0.16922881289966085 2216 16 0.16456238206561125 2216 17 0.49531067291528075 2216 18 0.34534239079984275 2216 19 -1.143765372819729 2216 20 -0.7381107781208343 2216 21 -0.6522827707466945 2216 22 -0.4608065905304173 2216 23 -4.3439504866523215 2216 24 -1.8478073986179595 2216 25 -1.0155407812830362 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 29.0 4 48.0 5 69.0 6 103.0 7 358.0 8 271.0 9 781.0 10 2069.0 11 4390.0 12 8217.0 13 13306.0 14 18496.0 15 23963.0 16 27860.0 17 30302.0 18 32086.0 19 33510.0 20 34540.0 21 36026.0 22 38102.0 23 39858.0 24 43719.0 25 47929.0 26 53581.0 27 61464.0 28 71517.0 29 86526.0 30 104533.0 31 126735.0 32 164267.0 33 213319.0 34 280658.0 35 374356.0 36 508835.0 37 719015.0 38 1084709.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.03139055761117 20.835080937830742 13.465997143425628 23.667531361132465 2 15.85953288567296 20.99839263304538 40.30049616689543 22.841578314386226 3 18.190607483332688 26.072200356011273 29.99142883952743 25.745763321128614 4 13.311704606811366 17.236879608778075 37.71055152085446 31.7408642635561 5 13.77398222898739 37.0610826029911 35.355420252230715 13.809514915790801 6 31.143055786671347 38.513581478954876 17.27402340709616 13.069339327277621 7 26.31310176172517 32.571902160454854 23.35735809608676 17.75763798173321 8 24.16407258087105 36.840818990388655 21.075282019297383 17.91982640944291 9 24.829170415028244 17.070283705259502 21.9177263617465 36.18281951796576 10 15.52606992139334 29.580625216915777 33.49877476866013 21.394530093030756 11 32.433878870530044 25.473897561058724 23.545491528461863 18.546732039949372 12 21.137181646878048 27.15045745575158 31.33944145635278 20.372919441017594 13 26.588910680979893 24.009056982855824 26.765597002541064 22.63643533362322 14 21.428338001867413 23.078221288515408 27.750793650793646 27.74264705882353 15 22.915250581684138 30.621048189216342 24.100863842962088 22.36283738613743 16 22.515821621501768 30.836284874378133 25.39635452102178 21.25153898309832 17 20.793425766089918 29.893891539798023 28.18056970220918 21.132112991902876 18 20.895667655675535 29.71297892085377 28.79703316011674 20.594320263353953 19 21.51923018127783 28.436998293248916 29.12408638172271 20.919685143750545 20 22.615159062521588 28.172592031097427 28.425856084778577 20.786392821602405 21 23.016607760245225 28.560073464806084 27.476537585953004 20.94678118899568 22 22.108924824787213 28.14352628716927 28.843490579468305 20.904058308575216 23 22.34362440918346 28.38902077788472 28.210923956321356 21.05643085661047 24 22.01902629009281 27.65834548479185 28.518500712283956 21.804127512831382 25 22.647416291961473 27.888718338880814 27.889933030766045 21.573932338391668 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2973.0 1 2973.0 2 3386.0 3 3799.0 4 3799.0 5 3799.0 6 5516.0 7 7233.0 8 7233.0 9 7233.0 10 11142.5 11 15052.0 12 15052.0 13 15052.0 14 24837.5 15 34623.0 16 34623.0 17 34623.0 18 55924.5 19 77226.0 20 77226.0 21 77226.0 22 112484.5 23 147743.0 24 147743.0 25 147743.0 26 194739.0 27 241735.0 28 241735.0 29 241735.0 30 293982.0 31 346229.0 32 346229.0 33 346229.0 34 393314.0 35 440399.0 36 440399.0 37 440399.0 38 474507.5 39 508616.0 40 508616.0 41 508616.0 42 511999.0 43 515382.0 44 515382.0 45 515382.0 46 499484.5 47 483587.0 48 483587.0 49 483587.0 50 462413.0 51 441239.0 52 441239.0 53 441239.0 54 404158.5 55 367078.0 56 367078.0 57 367078.0 58 320045.5 59 273013.0 60 273013.0 61 273013.0 62 228508.5 63 184004.0 64 184004.0 65 184004.0 66 144587.5 67 105171.0 68 105171.0 69 105171.0 70 78710.0 71 52249.0 72 52249.0 73 52249.0 74 37867.5 75 23486.0 76 23486.0 77 23486.0 78 16508.0 79 9530.0 80 9530.0 81 9530.0 82 6555.5 83 3581.0 84 3581.0 85 3581.0 86 2397.5 87 1214.0 88 1214.0 89 1214.0 90 760.0 91 306.0 92 306.0 93 306.0 94 182.0 95 58.0 96 58.0 97 58.0 98 41.5 99 25.0 100 25.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08164644406285212 2 0.021094137019953794 3 0.03306459309433023 4 0.040648215363672024 5 0.050658596759203194 6 0.01824736189115472 7 0.022027505914642014 8 0.03376461976534639 9 0.03399796198901845 10 0.024594270375034623 11 0.023987580593487278 12 0.017850680110912225 13 0.026180997496004596 14 0.03619137889153577 15 0.03357794598640875 16 0.024874281043441086 17 0.024897615265808293 18 0.026297668607840628 19 0.01871404633849883 20 0.01621728454520784 21 0.011807116517805995 22 0.017757343221443404 23 0.018737380560866036 24 0.017337327218833704 25 0.10803744956016158 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4285551.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.90950363304412 #Duplication Level Percentage of deduplicated Percentage of total 1 88.05146555846602 70.361489069391 2 8.75063719499283 13.985181494494611 3 1.6408407585522082 3.9335631167032368 4 0.5571209806611717 1.7807704411275598 5 0.28050817101681247 1.1207634355483267 6 0.1672100630279849 0.8017003883409778 7 0.112916075834026 0.6316147300461731 8 0.08238797363110893 0.5266865662555393 9 0.05739173948743951 0.41275308735704425 >10 0.27860833739022606 3.8083412987588843 >50 0.013709603085373386 0.7617821396512731 >100 0.006265454195405698 0.823212078252337 >500 6.449367677920557E-4 0.37436631670728143 >1k 2.931528915002922E-4 0.6777758373657782 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGGTATCAACGCAGAGTACATGGG 4889 0.11408101315326781 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.3334222367205525E-5 0.0 10 0.0 0.0 0.0 2.3334222367205525E-5 0.0 11 0.0 0.0 0.0 4.666844473441105E-5 0.0 12 0.0 0.0 0.0 4.666844473441105E-5 0.0 13 0.0 0.0 0.0 4.666844473441105E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 70 0.0014887379 10.860279 5 TGGTATC 1505 0.0 10.102586 2 GTGGTAT 1615 0.0 9.891329 1 ACCCGTG 135 6.468963E-7 9.849868 7 GGTATCA 3040 0.0 9.665021 1 GTCCTAG 360 0.0 9.508629 1 CCTACAC 540 0.0 9.502745 3 CGTAGAC 140 1.0556978E-6 9.502745 3 TCGCGTT 80 0.004526135 9.497975 10 CTACACT 585 0.0 9.421523 4 CTAGTAC 295 0.0 9.341681 3 TATACTG 775 0.0 9.073588 5 GTCCTAA 390 0.0 9.021007 1 GTCCAAG 630 0.0 8.904906 1 GTCTTAG 330 0.0 8.644209 1 CCGCACT 100 0.0028989695 8.552469 4 CCGATCC 100 0.0029121719 8.548178 9 CTGTCGC 315 0.0 8.442644 9 GTTAGAC 260 6.002665E-11 8.406274 3 GGACCGT 125 2.7511982E-4 8.358315 6 >>END_MODULE