FastQCFastQC Report
Wed 25 May 2016
SRR1295297_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295297_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4285551
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT120740.2817374008616395No Hit
GGTATCAACGCAGAGTACTTTTTTT98490.2298187560946072No Hit
TATCAACGCAGAGTACTTTTTTTTT68770.1604694472192724No Hit
GTGGTATCAACGCAGAGTACATGGG59150.13802192530202068No Hit
ACGCAGAGTACTTTTTTTTTTTTTT47980.1119575989178521No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTCG500.00150453913.2950179
AGACCGT658.0537447E-411.6879276
AGCGTCA4200.011.5440543
GGTATCA39850.011.2039391
GTGGTAT16950.010.5587971
CAGCGTC4600.010.5402232
GCAGCGT4350.010.2857241
TGGTATC17050.010.0923352
GTCTAGC1701.6119884E-89.5197661
TAGGACA4900.09.1187264
GTCCTAG4700.09.114671
CTAGGAC3250.09.06808953
GCGTCAG5350.09.0625164
AAGACCG1054.4637112E-49.0540555
GTACCGT1054.5124115E-49.044236
CTAGACA5200.08.9582894
GTCTTAC3900.08.7874771
TGCACCG1752.6002454E-78.6918925
GTATTAG5600.08.6697871
ATTATAC6600.08.6426083
Produced by FastQC (version 0.11.5)