FastQCFastQC Report
Wed 25 May 2016
SRR1295296_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295296_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4533473
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG59480.1312018401786004No Hit
ATTCCATTCCATTCCATTCCATTCC49140.10839371934055855No Hit
GTATCAACGCAGAGTACTTTTTTTT45480.10032043865707371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36200.010.4796041
TCCGTAC1659.276846E-1010.3660883
TCGACCG1106.852033E-59.4987869
GTATACG1054.458898E-49.0550581
GTCCTAA4800.08.9135711
TGGTATC16850.08.910022
GTATTAT7400.08.7369061
GTGGTAT18450.08.6575181
GTCCTAG4350.08.5242431
GTACCGT1451.6941904E-58.5158716
GGTCTAG2053.8135113E-88.3483211
TATACTG12000.08.3144665
ATAGGAC2752.1827873E-118.292873
TGGACTG10250.08.1580275
TAATACG1050.0045056938.1447834
TCTATAC5650.08.0727053
TAGGACT4250.08.0489634
CTAAGAC4750.08.0018923
CTATACT7250.07.99499374
GTCTATA3450.07.9920721
Produced by FastQC (version 0.11.5)