##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295296_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4533473
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.799055823206622	31.0	30.0	33.0	25.0	34.0
2	29.759493880298834	31.0	30.0	33.0	25.0	34.0
3	30.08226650958327	31.0	30.0	34.0	26.0	34.0
4	33.508737561688356	35.0	33.0	37.0	27.0	37.0
5	33.6629438401861	35.0	33.0	37.0	28.0	37.0
6	33.933015813703975	35.0	35.0	37.0	29.0	37.0
7	33.88732236852409	35.0	35.0	37.0	28.0	37.0
8	33.974847319042155	36.0	35.0	37.0	28.0	37.0
9	34.87173586343185	38.0	34.0	39.0	27.0	39.0
10	34.793326220317184	38.0	34.0	39.0	27.0	39.0
11	35.181706166552665	38.0	35.0	39.0	27.0	39.0
12	35.18031253301828	38.0	35.0	39.0	27.0	39.0
13	35.07574876921071	38.0	35.0	39.0	27.0	39.0
14	35.16837598900446	38.0	33.0	40.0	24.0	41.0
15	35.80239697026981	39.0	34.0	41.0	27.0	41.0
16	35.89045065449822	39.0	34.0	41.0	27.0	41.0
17	35.685507336207806	39.0	34.0	40.0	27.0	41.0
18	35.63446787926166	39.0	34.0	40.0	27.0	41.0
19	35.70222013012982	39.0	34.0	41.0	26.0	41.0
20	35.670482210878944	39.0	34.0	40.0	26.0	41.0
21	35.65690233514129	39.0	34.0	40.0	26.0	41.0
22	35.38181279562049	39.0	34.0	40.0	25.0	41.0
23	35.04879393789265	38.0	33.0	40.0	24.0	41.0
24	35.38760416131297	39.0	34.0	40.0	25.0	41.0
25	33.91771010878415	38.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3763817028651175
1101	2	0.6299550422078646
1101	3	0.38577229474327623
1101	4	0.3640052279530366
1101	5	0.21773645004501674
1101	6	0.27430100683002934
1101	7	0.24213010416862346
1101	8	0.2850109099288929
1101	9	0.12762487257182187
1101	10	0.6014191349667612
1101	11	0.4874378895691507
1101	12	0.5134526193857738
1101	13	0.42948812864129593
1101	14	0.9678316477879321
1101	15	0.684184780694828
1101	16	0.44656515051292445
1101	17	0.786648981350119
1101	18	0.7804172458290566
1101	19	0.837138766243875
1101	20	0.8356767227091453
1101	21	0.9177120465657609
1101	22	0.7430020877193755
1101	23	-1.7241317597657329
1101	24	0.02722489851446852
1101	25	0.5788550979504805
1102	1	0.34426951467968436
1102	2	0.4521590699157265
1102	3	0.36029653133994444
1102	4	0.48100613963610783
1102	5	0.1963082904621345
1102	6	0.22136389219815555
1102	7	0.17715478045700195
1102	8	0.35376971155943693
1102	9	0.5446733949575986
1102	10	1.035971604821576
1102	11	0.6673055755726622
1102	12	0.531042692740975
1102	13	0.5963734468719508
1102	14	1.1132126910952138
1102	15	0.7548885414124413
1102	16	0.5443925825090119
1102	17	0.8737969794277021
1102	18	0.9900366646296774
1102	19	0.9272100782821013
1102	20	0.9659308734538499
1102	21	1.088221612473113
1102	22	0.8349623428029389
1102	23	0.9923043811743781
1102	24	0.9433829418031578
1102	25	0.7651570035340995
1103	1	-0.4126397509330708
1103	2	-1.644736559051644
1103	3	-0.5605096197626906
1103	4	-0.18865692531176848
1103	5	-0.39343295621523566
1103	6	-0.21218985976442895
1103	7	-0.13482433728484722
1103	8	0.08410856366573682
1103	9	0.7034438219686407
1103	10	0.7760735665431184
1103	11	0.39127060594838525
1103	12	0.213263868393625
1103	13	0.06135985815291889
1103	14	-1.0914148433835393
1103	15	-0.4036166931039915
1103	16	-0.18217678726794873
1103	17	0.39660619653898976
1103	18	0.5454065783388131
1103	19	0.4953668498606234
1103	20	0.4948364080531604
1103	21	0.6725187234937664
1103	22	0.07712477565544873
1103	23	1.0643488960244127
1103	24	0.7557134768764087
1103	25	0.6921544941101843
1104	1	0.0791078970312249
1104	2	0.2618083385255794
1104	3	-0.23493094359823274
1104	4	-0.03356836546334563
1104	5	-0.1187283523074214
1104	6	-0.10047772965690882
1104	7	-0.07720198458183347
1104	8	0.046735950386846525
1104	9	-2.2182674790593495
1104	10	-0.5487711832602287
1104	11	-0.3626281236726001
1104	12	0.08243188371879029
1104	13	0.2157202435198684
1104	14	-1.754523320912675
1104	15	-0.46505350814444313
1104	16	-0.1047582398377429
1104	17	0.3807772933887321
1104	18	0.554232927519692
1104	19	0.5958153616628792
1104	20	0.6471420916966366
1104	21	0.7531132970896266
1104	22	0.8527061817159094
1104	23	1.3056769151601983
1104	24	1.0040794599905993
1104	25	0.8009823711340971
1105	1	-0.21927197059715198
1105	2	-0.08411817136423849
1105	3	-1.500076039973763
1105	4	-0.578369330694045
1105	5	-0.5555024081140374
1105	6	-0.1997239113294853
1105	7	-0.12181969252744551
1105	8	-0.20805825824677981
1105	9	0.36816484586118037
1105	10	0.5502001903718394
1105	11	-0.14547155661496447
1105	12	-0.1399864929078447
1105	13	-0.22506250698260288
1105	14	0.6071254400967234
1105	15	0.32557754716249576
1105	16	-0.2238136327625071
1105	17	0.2989326760724538
1105	18	0.17565103759432077
1105	19	0.15765549357073638
1105	20	-0.37000317950211326
1105	21	0.03787019422846072
1105	22	0.11619337268815855
1105	23	0.3658601208172456
1105	24	0.26903771627316075
1105	25	0.5030459928116571
1106	1	-1.7059991195767914
1106	2	-1.1388018851501656
1106	3	-1.2927454646272913
1106	4	-1.6757923527352112
1106	5	-1.1846875953195237
1106	6	-0.9564856859787341
1106	7	-0.8388495464748118
1106	8	-0.9393622686505978
1106	9	-0.4203495728230493
1106	10	-2.084295183574234
1106	11	-1.4192999804093631
1106	12	-1.0425788683180457
1106	13	-1.1081724450332757
1106	14	-0.440959288036602
1106	15	-1.1801075588406462
1106	16	-1.5796591226853565
1106	17	-0.8286841034303407
1106	18	-0.7686523641018752
1106	19	-0.647381878101676
1106	20	-0.29135160944557015
1106	21	-0.3754220461750961
1106	22	-0.3952267260687634
1106	23	-0.6920599835184689
1106	24	-0.8949567446114841
1106	25	-1.6541270252003812
1107	1	-0.04733579256395615
1107	2	0.07561406673482196
1107	3	-0.078419041923798
1107	4	0.17010828948668433
1107	5	-0.08835924935384298
1107	6	-9.738899898934505E-4
1107	7	-0.11435762746410916
1107	8	-0.17059985383166776
1107	9	0.405844912017848
1107	10	-0.0028303200283588126
1107	11	-0.11909536700294154
1107	12	-0.20237765840499122
1107	13	0.0384051001019472
1107	14	-1.1809091124682638
1107	15	-0.5658200167224194
1107	16	-0.2959216946995227
1107	17	0.003521458339314165
1107	18	0.3031118972564215
1107	19	0.2543008436315901
1107	20	0.2854879527613434
1107	21	0.36117463351521906
1107	22	-0.37874664198516683
1107	23	0.5201261841076175
1107	24	0.42674424061331706
1107	25	-0.06943743821718584
1108	1	-0.25143870234084886
1108	2	0.11232500047447047
1108	3	-0.19538240590867773
1108	4	-0.45274747820893424
1108	5	-0.5205091110546647
1108	6	-0.4584715922798779
1108	7	-0.3704132191055791
1108	8	-0.3381706434987777
1108	9	0.13934385596374455
1108	10	0.20673997260060162
1108	11	-0.11707090639627182
1108	12	-0.14484264601652797
1108	13	-0.17092919979884869
1108	14	-0.027181375128996876
1108	15	-0.1480568140474361
1108	16	-0.2402183292509079
1108	17	0.12111191345306338
1108	18	0.20288705113662786
1108	19	0.21533067234980052
1108	20	0.23672143983289118
1108	21	0.21511167693325461
1108	22	0.09558947580200794
1108	23	-0.08419957678379575
1108	24	0.03443036037430858
1108	25	-0.7937675737517296
1109	1	-1.0273636602987963
1109	2	-0.849777722508648
1109	3	-0.9160995937568615
1109	4	-0.8424832387769854
1109	5	-1.1479080288033003
1109	6	-1.401182288419058
1109	7	-1.1001192442803926
1109	8	-1.5138945979949696
1109	9	-1.2105353264310068
1109	10	-1.3280954735024508
1109	11	-1.4131205183611968
1109	12	-1.31691975113187
1109	13	-1.3942469694981696
1109	14	-0.7707893807079884
1109	15	-1.5870954158954689
1109	16	-1.6445961190524514
1109	17	-1.6174224461442819
1109	18	-2.310532202352448
1109	19	-2.474332408365129
1109	20	-2.1091591886622965
1109	21	-1.8745206863295678
1109	22	-1.984192668306136
1109	23	-1.3807467964795492
1109	24	-2.3760300659329445
1109	25	-1.4306721841620416
1110	1	-0.016552526805750034
1110	2	0.09366951741826668
1110	3	-0.11467501824357385
1110	4	0.17702089462613912
1110	5	0.1293470422967573
1110	6	0.07670489096097555
1110	7	-0.02432714082088694
1110	8	-0.01850288450097537
1110	9	0.20203359937666932
1110	10	0.1064024832080932
1110	11	0.12753260298590874
1110	12	0.08735229645198217
1110	13	0.07446579732058467
1110	14	-2.7007459852918387
1110	15	-0.8367985205089781
1110	16	-0.3334615440130051
1110	17	-0.4416679886093675
1110	18	-3.003477079988265
1110	19	-0.7581140664796919
1110	20	-2.3040475884567897
1110	21	-0.49172114285636326
1110	22	-2.108355680756155
1110	23	-0.7366663289686528
1110	24	-0.024145027408366104
1110	25	-0.01986384892899906
1111	1	-0.25021767001454265
1111	2	-1.3981633742255681
1111	3	-0.36848631969368384
1111	4	0.011227681349161855
1111	5	-0.0798019225063058
1111	6	0.04772473231891183
1111	7	-0.10112015695357712
1111	8	-0.0844078257380545
1111	9	-1.78014692033301
1111	10	-0.5062209560034105
1111	11	-0.3540347562320747
1111	12	-0.2381663050994831
1111	13	-0.1555938780222874
1111	14	0.8048475675671511
1111	15	0.14354206177901574
1111	16	0.20135373150272073
1111	17	-0.22049187155511163
1111	18	0.006108408087307282
1111	19	-0.12966863148542274
1111	20	0.20651983322587597
1111	21	-0.7415188340901011
1111	22	-0.31953345009073786
1111	23	-0.12192603492308507
1111	24	-0.6601274174881482
1111	25	-2.134258728354098
1112	1	0.14213079696757802
1112	2	0.38497776555319163
1112	3	-0.021042017091996712
1112	4	0.303419568396329
1112	5	0.18150051163364367
1112	6	0.18606534145342124
1112	7	0.10392825118865545
1112	8	0.006174218062859893
1112	9	-0.4287735527562475
1112	10	0.42132753970606274
1112	11	0.03566013655517963
1112	12	0.0033343389278144286
1112	13	-0.03147718887047546
1112	14	0.41644210573575435
1112	15	0.03945470522717187
1112	16	0.10893567834001061
1112	17	-4.107872951048787
1112	18	-1.2813642177750495
1112	19	-1.4974500798820216
1112	20	-0.24317114043098798
1112	21	-0.7108738106931014
1112	22	-1.926980450734014
1112	23	-1.6472177397156855
1112	24	-0.48883659163244175
1112	25	-0.21334803256022639
1113	1	-0.4835912375584641
1113	2	-0.2854771874942834
1113	3	-0.22465921034693181
1113	4	-0.7114300489937904
1113	5	-0.343102730434623
1113	6	-0.4217866507867072
1113	7	-0.6381663684364725
1113	8	-0.4500795696088602
1113	9	6.951805940147437E-4
1113	10	0.13230047318158
1113	11	-0.289507929048888
1113	12	-0.32559932735009056
1113	13	-0.6383375314909117
1113	14	-1.8373354001390325
1113	15	-2.217972671085377
1113	16	-0.7739198645358414
1113	17	-0.5295509313617472
1113	18	-0.8816251469979974
1113	19	-0.3362023067125861
1113	20	-0.011171982993140261
1113	21	-1.8714818329763574
1113	22	-2.3334811525349295
1113	23	-0.943374029415132
1113	24	-2.845565096467361
1113	25	-1.0937009984461383
1114	1	0.18244284903605035
1114	2	0.20858466041130796
1114	3	-0.0239672984125896
1114	4	0.3313244288289212
1114	5	0.0840851255177597
1114	6	0.18391945010125
1114	7	0.14448291559813953
1114	8	-0.008048299079362664
1114	9	0.4783948320516558
1114	10	0.310349287734077
1114	11	0.22137535529815722
1114	12	0.2434612532227831
1114	13	0.1427816939981028
1114	14	0.9173908715129571
1114	15	0.38395708868007006
1114	16	0.33722143840633123
1114	17	-0.5595172461754672
1114	18	-0.252998428727615
1114	19	-0.6400650333711653
1114	20	-1.799495607006044
1114	21	-0.9226951100711602
1114	22	-1.659542841118693
1114	23	-3.24731248566939
1114	24	-2.1214138731550634
1114	25	-1.5450653726372465
1115	1	0.2830197318821597
1115	2	0.30285819939375
1115	3	0.23767961729026155
1115	4	0.4519961373971668
1115	5	0.45232729852811104
1115	6	0.22179225283948512
1115	7	0.3864673820408342
1115	8	0.38402582460896184
1115	9	0.6195324770195114
1115	10	0.8243468054589584
1115	11	0.5395480878927827
1115	12	0.44049017032147475
1115	13	0.3175639235804226
1115	14	1.0053218635615906
1115	15	0.5040501564170015
1115	16	0.539529233488544
1115	17	0.5234687092807064
1115	18	0.7779000950922068
1115	19	0.11064387416409005
1115	20	0.6356807101204254
1115	21	-0.3708076492522565
1115	22	0.6991474071615542
1115	23	0.8296201114197359
1115	24	0.8071591878312034
1115	25	0.7573355956610115
1116	1	0.4316685882643725
1116	2	0.5397402254251418
1116	3	0.41575513991575974
1116	4	0.7605852179515935
1116	5	0.6532045870471848
1116	6	0.41661551262821206
1116	7	0.4548030395170741
1116	8	0.378260961000386
1116	9	0.7122603679115116
1116	10	0.49364785026975966
1116	11	0.45191010458209746
1116	12	0.4988158397700886
1116	13	-0.1627297681546409
1116	14	0.9199642012645484
1116	15	0.5992214688469915
1116	16	0.6750495660046951
1116	17	0.8350003060999285
1116	18	0.7440841966885614
1116	19	0.9490590332538673
1116	20	0.7336852116078489
1116	21	-0.07038657331703035
1116	22	0.12938591595901983
1116	23	-0.9428772901795952
1116	24	-0.4626600631943347
1116	25	-1.1279141243190907
1201	1	0.3540810063353561
1201	2	0.59430499709336
1201	3	0.4325771852510911
1201	4	0.39094738113229255
1201	5	0.3492359988999638
1201	6	0.3512178481463195
1201	7	0.3084190184128701
1201	8	0.36321606873672607
1201	9	0.5867678974874408
1201	10	0.9754232310773645
1201	11	0.5987226265444221
1201	12	0.5553933737766812
1201	13	0.5028059495868433
1201	14	1.0892527594666177
1201	15	0.7429097540796548
1201	16	0.5069426801476666
1201	17	0.5676741738625424
1201	18	0.569318161009015
1201	19	0.8850032986045804
1201	20	0.9206501075382576
1201	21	0.8156370466851115
1201	22	0.8995765362005201
1201	23	0.36056442626833984
1201	24	0.847849951625463
1201	25	0.9374277036592531
1202	1	0.37440602300790715
1202	2	0.7361858177751692
1202	3	0.4781485633845506
1202	4	0.5391245416987687
1202	5	0.2919895982095255
1202	6	0.2145551044328542
1202	7	0.2907776940238733
1202	8	0.4455068772573796
1202	9	0.9067072486309797
1202	10	0.04994980908529101
1202	11	0.4228256432954325
1202	12	0.42697777611476084
1202	13	0.39466397791056096
1202	14	1.1473186258508292
1202	15	0.7770017081026452
1202	16	0.6081872370501031
1202	17	-1.5629098361802178
1202	18	-0.0036942355230991097
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2210	21	1.0152082651005898
2210	22	1.1842668205190492
2210	23	1.5536258327799999
2210	24	1.2556769734512798
2210	25	1.050386522344894
2211	1	0.08622785404883615
2211	2	-0.5802502049193663
2211	3	-0.05900913264146723
2211	4	0.26914765492244186
2211	5	0.2503406311332057
2211	6	0.2512063785592247
2211	7	0.2734623068544835
2211	8	0.3100221587174872
2211	9	0.7291754748146602
2211	10	0.9005936469423901
2211	11	0.42186177853495366
2211	12	0.3425359296446686
2211	13	0.24238616269916946
2211	14	-0.9351864887204471
2211	15	-0.0556610243723199
2211	16	0.22661672188216642
2211	17	0.35525793884700363
2211	18	0.32508358945359106
2211	19	0.6116438195458258
2211	20	0.7110358186526611
2211	21	0.7084240083544913
2211	22	0.06809640448526011
2211	23	0.5774698946714807
2211	24	-0.1605390153717252
2211	25	0.4145319289071452
2212	1	-0.01689059981860197
2212	2	-1.1902267077118225
2212	3	-0.421115640853543
2212	4	0.11835410061772222
2212	5	0.19708216803442014
2212	6	0.10433990645172742
2212	7	0.059940252817938244
2212	8	-0.031172917016661472
2212	9	-2.4589933340267436
2212	10	-0.15865176914848433
2212	11	-0.04699047184481486
2212	12	-0.0925402444286263
2212	13	-0.047021555314103125
2212	14	0.6430640754486561
2212	15	0.29112711199749697
2212	16	0.3108562067482623
2212	17	0.24272665233905144
2212	18	0.08136380075136174
2212	19	-0.6016955893106442
2212	20	0.2894044099783386
2212	21	0.2946806718465922
2212	22	-1.7081874597161146
2212	23	-0.4083443936209008
2212	24	0.20333519459475013
2212	25	-0.5208561263932339
2213	1	0.13970527258702603
2213	2	0.4903975335646358
2213	3	0.19277740184904602
2213	4	0.32029862475039295
2213	5	0.3576588563548455
2213	6	0.2428440619594454
2213	7	0.2309432036678274
2213	8	0.23906175634422766
2213	9	0.7717524171041177
2213	10	0.672854197304467
2213	11	0.06000219262045192
2213	12	0.0828788171199335
2213	13	0.06992263035967738
2213	14	-1.6316151612840102
2213	15	-0.3510663837421717
2213	16	-0.07755600479331548
2213	17	-2.152548357785328
2213	18	-0.8822625854348658
2213	19	-0.5645486712537249
2213	20	0.16619610730163714
2213	21	-0.21186641615195612
2213	22	0.3673849835208003
2213	23	0.8172101794012789
2213	24	0.6842864238090058
2213	25	-0.958161197060619
2214	1	0.3853706258690366
2214	2	0.27331612277472317
2214	3	0.27619570459880904
2214	4	0.5558652155766737
2214	5	0.5828432143726587
2214	6	0.4457287522278719
2214	7	0.4389149907791463
2214	8	0.351904883200568
2214	9	0.8596300624033901
2214	10	0.7473521363742819
2214	11	0.1984186921196951
2214	12	0.041324042733968724
2214	13	0.18921390342097766
2214	14	1.1030690555056921
2214	15	0.767273780890946
2214	16	0.6956502277916243
2214	17	0.5903049702968417
2214	18	0.4526551709193214
2214	19	-0.6953623182424877
2214	20	0.3154282107352344
2214	21	0.6187157580250826
2214	22	0.8381361831029182
2214	23	1.5245191193868664
2214	24	1.116847214753534
2214	25	1.1475336055248064
2215	1	0.05546313351546672
2215	2	0.4454381629814854
2215	3	0.3281514675449344
2215	4	0.6507376207980329
2215	5	0.6323362078152499
2215	6	0.4795852573620678
2215	7	0.3230848713271186
2215	8	0.2819222023955632
2215	9	0.5115645924007026
2215	10	0.8754408832451546
2215	11	0.5308453466683645
2215	12	0.4172601844149284
2215	13	0.2782621125877114
2215	14	1.2473817235754439
2215	15	0.7714764074446023
2215	16	0.6550260584721173
2215	17	0.8641458059838598
2215	18	0.7826201022779955
2215	19	-0.13490984897411806
2215	20	-1.3741260714246764
2215	21	-1.6914770586175862
2215	22	-0.0163348273824937
2215	23	0.7091626281884871
2215	24	0.944608149789822
2215	25	0.0598195723947299
2216	1	0.07549252474427703
2216	2	-0.21158509288791905
2216	3	0.08236018341925799
2216	4	0.3573261479660417
2216	5	0.5238762549540752
2216	6	0.147265516081184
2216	7	0.3131739351751577
2216	8	0.07373804067545109
2216	9	0.4409534964567854
2216	10	-1.6677183837544476
2216	11	-0.38444121185161606
2216	12	-0.07088687664101911
2216	13	0.11129712256427382
2216	14	-0.47659352754749307
2216	15	-0.3857245032225194
2216	16	0.04103088998320459
2216	17	0.41882714203695315
2216	18	0.2362817860266304
2216	19	-1.3117424791195162
2216	20	-1.0131868793973453
2216	21	-0.8977501546354176
2216	22	-0.803451625725522
2216	23	-4.374012661720066
2216	24	-1.9719601818367067
2216	25	-1.1929750581822418
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	26.0
4	59.0
5	80.0
6	110.0
7	356.0
8	176.0
9	545.0
10	1532.0
11	3622.0
12	7229.0
13	11870.0
14	17230.0
15	21829.0
16	25874.0
17	28829.0
18	30780.0
19	32503.0
20	33435.0
21	34759.0
22	37270.0
23	39984.0
24	43517.0
25	48501.0
26	54482.0
27	62830.0
28	73666.0
29	90131.0
30	109262.0
31	134558.0
32	174999.0
33	227793.0
34	300490.0
35	404857.0
36	552525.0
37	783904.0
38	1143857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58930727832186	20.483573077057233	13.603379298134225	23.323740346486677
2	16.04741942380189	20.68096862422293	40.07489785603012	23.19671409594506
3	18.434101165295843	25.743545772040488	30.110205760372526	25.71214730229114
4	13.252126725029918	16.84777104035035	37.27957935738932	32.620522877230414
5	14.166881650760049	36.83126613897911	34.72114117161292	14.280711038647926
6	31.82113715311292	37.43599473595381	17.284096336898955	13.45877177403431
7	26.404904039270793	32.44023566182092	23.640424876120452	17.514435422787837
8	23.862802604806017	37.26983918228986	20.816337608845274	18.05102060405885
9	24.854603565776383	16.998915208877456	21.7309082499733	36.415572975372854
10	15.93135329726047	29.197133116284242	32.99950047525455	21.87201311120074
11	32.705976368816444	25.402761141390624	23.35252330938944	18.5387391804035
12	21.3444296922468	26.840873500714153	31.05460416382617	20.76009264321288
13	26.397319145349606	23.989461388800265	26.814569954323268	22.798649511526854
14	21.59702393995625	22.62923553538915	27.382184199103676	28.39155632555092
15	23.14147919049838	30.23818917792936	23.746076593370898	22.87425503820136
16	22.939397235562737	30.483605528345002	25.046736230269893	21.530261005822364
17	21.13000266307119	29.732803719739003	27.822739627315652	21.314453989874153
18	21.17019804848579	29.689841936748664	28.29594104196817	20.844018972797375
19	21.912329502132106	28.110703594928125	28.70731375843664	21.26965314450312
20	22.953669252669744	27.837519731464553	27.888658834015224	21.32015218185047
21	23.35853427165832	28.365152551346817	27.099009464139957	21.177303712854904
22	22.478224226485693	27.784629436786272	28.505987786151753	21.231158550576275
23	22.667483274396965	28.14808016287441	27.96229160838482	21.222144954343804
24	22.322544889828063	27.165419736190977	28.096607750479468	22.415427623501493
25	23.05590420913444	27.394263748163866	27.52083898269903	22.028993060002666
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4192.0
1	4192.0
2	4409.5
3	4627.0
4	4627.0
5	4627.0
6	6075.0
7	7523.0
8	7523.0
9	7523.0
10	11130.0
11	14737.0
12	14737.0
13	14737.0
14	24751.0
15	34765.0
16	34765.0
17	34765.0
18	54044.5
19	73324.0
20	73324.0
21	73324.0
22	105809.5
23	138295.0
24	138295.0
25	138295.0
26	183877.5
27	229460.0
28	229460.0
29	229460.0
30	283495.5
31	337531.0
32	337531.0
33	337531.0
34	392018.0
35	446505.0
36	446505.0
37	446505.0
38	493882.0
39	541259.0
40	541259.0
41	541259.0
42	552857.5
43	564456.0
44	564456.0
45	564456.0
46	547336.5
47	530217.0
48	530217.0
49	530217.0
50	507086.5
51	483956.0
52	483956.0
53	483956.0
54	443809.0
55	403662.0
56	403662.0
57	403662.0
58	350784.0
59	297906.0
60	297906.0
61	297906.0
62	250275.5
63	202645.0
64	202645.0
65	202645.0
66	159366.0
67	116087.0
68	116087.0
69	116087.0
70	87903.5
71	59720.0
72	59720.0
73	59720.0
74	43223.5
75	26727.0
76	26727.0
77	26727.0
78	18687.5
79	10648.0
80	10648.0
81	10648.0
82	7231.0
83	3814.0
84	3814.0
85	3814.0
86	2443.0
87	1072.0
88	1072.0
89	1072.0
90	673.0
91	274.0
92	274.0
93	274.0
94	164.0
95	54.0
96	54.0
97	54.0
98	35.5
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08326949338840223
2	0.019852329549552846
3	0.030991692241246392
4	0.03838117046246884
5	0.046983846600608406
6	0.018484724625028096
7	0.023072818565369198
8	0.03741061212893515
9	0.038050298303309624
10	0.025697737694698967
11	0.02364633030791184
12	0.017977387314317302
13	0.026183016861465812
14	0.04067521743263939
15	0.037432670272879096
16	0.024749237505109215
17	0.024528656065669743
18	0.02867558712713189
19	0.019080294511514682
20	0.016278910230633336
21	0.01263931647988198
22	0.020139085420824168
23	0.019874387693496795
24	0.019322934094898103
25	0.10898928922704514
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4533473.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.6671333425186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.56923493881946	53.713199289709856
2	12.037698024755846	16.050376387067413
3	3.4466988381918378	6.893445931117176
4	1.382461621246169	3.6865901297813113
5	0.7327037254694584	2.442362848321626
6	0.4348664640256678	1.7394780326033255
7	0.28759349270835627	1.3421123608780048
8	0.20765103405870955	1.107479934104309
9	0.14625777679225183	0.8775528037010436
>10	0.7103974189054415	8.151442111934932
>50	0.031667111657054506	1.4309411131028682
>100	0.011703494674686407	1.3144793672582331
>500	4.330865856175488E-4	0.19846605132296247
>1k	5.996577878666751E-4	0.9199703246502879
>5k	3.331432154802286E-5	0.13210331444668888
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTATCAACGCAGAGTACATGGG	5948	0.1312018401786004	No Hit
ATTCCATTCCATTCCATTCCATTCC	4914	0.10839371934055855	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4548	0.10032043865707371	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.411628788789522E-5	0.0	0.0	0.0
6	0.0	4.411628788789522E-5	0.0	0.0	0.0
7	0.0	4.411628788789522E-5	0.0	0.0	0.0
8	0.0	6.617443183184283E-5	0.0	0.0	0.0
9	0.0	6.617443183184283E-5	0.0	0.0	0.0
10	0.0	6.617443183184283E-5	0.0	2.205814394394761E-5	0.0
11	0.0	6.617443183184283E-5	0.0	2.205814394394761E-5	0.0
12	0.0	8.823257577579044E-5	0.0	4.411628788789522E-5	0.0
13	0.0	8.823257577579044E-5	0.0	4.411628788789522E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3620	0.0	10.479604	1
TCCGTAC	165	9.276846E-10	10.366088	3
TCGACCG	110	6.852033E-5	9.498786	9
GTATACG	105	4.458898E-4	9.055058	1
GTCCTAA	480	0.0	8.913571	1
TGGTATC	1685	0.0	8.91002	2
GTATTAT	740	0.0	8.736906	1
GTGGTAT	1845	0.0	8.657518	1
GTCCTAG	435	0.0	8.524243	1
GTACCGT	145	1.6941904E-5	8.515871	6
GGTCTAG	205	3.8135113E-8	8.348321	1
TATACTG	1200	0.0	8.314466	5
ATAGGAC	275	2.1827873E-11	8.29287	3
TGGACTG	1025	0.0	8.158027	5
TAATACG	105	0.004505693	8.144783	4
TCTATAC	565	0.0	8.072705	3
TAGGACT	425	0.0	8.048963	4
CTAAGAC	475	0.0	8.001892	3
CTATACT	725	0.0	7.9949937	4
GTCTATA	345	0.0	7.992072	1
>>END_MODULE