FastQCFastQC Report
Wed 25 May 2016
SRR1295296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295296_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4533473
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT143690.3169534703305832No Hit
GGTATCAACGCAGAGTACTTTTTTT124420.27444742695059615No Hit
TATCAACGCAGAGTACTTTTTTTTT86770.1913985150016334No Hit
GTGGTATCAACGCAGAGTACATGGG71770.15831129908571198No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55590.12262122218440477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG759.718788E-713.9289669
AGCGTCA3500.011.6780743
GGTATCA52700.011.1426231
GTGGTAT20600.010.1640871
TAGACAG5850.09.7491375
GTCTAAG3700.09.5172811
TGGTATC22250.09.2704442
CGGAACT1356.8052905E-69.1533574
ACGAGCG850.0074046928.9462675
TACCGTC850.00745984878.9381777
CTAGACA4000.08.7925034
GGACCGT1304.3875854E-58.7666756
GCGTCAG4450.08.7577934
GGTCTAG1752.5568443E-78.7015141
TATACCG1107.108619E-48.6412815
GTTAGAC2106.324626E-98.6001323
TAGACGA1000.00289168748.5548685
TAAGACA5350.08.5282174
TACACCG1804.004305E-78.4492535
CAGCGTC4750.08.4047832
Produced by FastQC (version 0.11.5)