FastQCFastQC Report
Wed 25 May 2016
SRR1295295_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295295_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3992654
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49220.12327639710328017No Hit
GTGGTATCAACGCAGAGTACATGGG48110.12049629143922815No Hit
ATTCCATTCCATTCCATTCCATTCC43440.10879981085263085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACGG1103.8086E-812.0935095
GTCTAGC2002.5465852E-119.9835251
GTATAGC3950.09.1470521
CCTACAC3900.09.0145323
ATAACGC950.00182161149.001713
TCAGGAC4350.08.9557243
GTATTAG5900.08.8635021
GTATAGG5700.08.8408831
GGTATCA33150.08.834091
TAGCACT4950.08.8301814
TACACCG1201.7609047E-48.7102055
GTCTACG1107.088015E-48.6437451
GTGTAGG5500.08.6437451
GTCTTAA5500.08.6437451
CTAGAAC4300.08.6179153
GTATTAT8000.08.5573071
TGAACCG1000.0029008188.5518385
CTACACT5500.08.4654564
TATACTG10600.08.426345
GACTCCG2053.875175E-88.340337
Produced by FastQC (version 0.11.5)