##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295295_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3992654
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.05634698123103	31.0	30.0	33.0	26.0	34.0
2	30.03939234403983	31.0	30.0	34.0	25.0	34.0
3	30.410433761603183	31.0	30.0	34.0	26.0	34.0
4	33.90508068067005	35.0	35.0	37.0	28.0	37.0
5	34.069698751757606	35.0	35.0	37.0	30.0	37.0
6	34.236050256295684	36.0	35.0	37.0	30.0	37.0
7	34.235711383956634	37.0	35.0	37.0	30.0	37.0
8	34.15016653083388	37.0	35.0	37.0	30.0	37.0
9	35.21792847564552	39.0	35.0	39.0	27.0	39.0
10	35.142225196573506	38.0	35.0	39.0	27.0	39.0
11	35.4994422256474	39.0	35.0	39.0	29.0	39.0
12	35.51677681061269	39.0	35.0	39.0	29.0	39.0
13	35.42074795361682	39.0	35.0	39.0	28.0	39.0
14	35.63305761030132	39.0	34.0	41.0	25.0	41.0
15	36.245093363962916	39.0	36.0	41.0	27.0	41.0
16	36.34882186134837	39.0	36.0	41.0	27.0	41.0
17	36.16079129320998	39.0	36.0	41.0	27.0	41.0
18	36.13759769817269	39.0	35.0	41.0	27.0	41.0
19	36.20882350436577	39.0	35.0	41.0	27.0	41.0
20	36.17081820763833	39.0	35.0	41.0	27.0	41.0
21	36.1656048833683	39.0	35.0	41.0	27.0	41.0
22	35.9083101115198	39.0	34.0	41.0	27.0	41.0
23	35.58574747523828	39.0	34.0	41.0	25.0	41.0
24	35.911620440939785	39.0	34.0	41.0	27.0	41.0
25	34.60807222464055	38.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.33226243845543735
1101	2	0.5925449886638283
1101	3	0.3433960608424833
1101	4	0.4506643493580498
1101	5	0.37135138311095517
1101	6	0.23753513132297144
1101	7	0.32426850916910155
1101	8	0.2941309723515957
1101	9	0.263084513030158
1101	10	0.506043698735958
1101	11	0.4895207403935231
1101	12	0.5498828766770103
1101	13	0.5428706218104153
1101	14	0.9858711165363587
1101	15	0.707679743284352
1101	16	0.5180874774338733
1101	17	0.794152316790516
1101	18	0.763999161332066
1101	19	0.8411366301693448
1101	20	0.8270773097586783
1101	21	0.9286964478544704
1101	22	0.7003481410975994
1101	23	-1.5117656702544977
1101	24	0.13996098900399545
1101	25	0.6292047299641226
1102	1	0.1911810603663895
1102	2	0.31641602830035254
1102	3	0.21034893985208214
1102	4	0.4317820227915661
1102	5	0.19397640096906343
1102	6	0.14165310521664054
1102	7	0.15735492304297338
1102	8	0.10387900755608115
1102	9	0.2885375389403535
1102	10	0.7790110171865905
1102	11	0.503001594483294
1102	12	0.3797281699656665
1102	13	0.370092059531423
1102	14	0.8696994541058629
1102	15	0.6243703552867785
1102	16	0.4832343265774526
1102	17	0.8009848341663499
1102	18	0.7230809125244235
1102	19	0.8915208115576903
1102	20	0.8456880316459774
1102	21	0.8643806716986973
1102	22	0.5695398165150749
1102	23	0.6734790625115181
1102	24	0.7540274124909772
1102	25	0.6637889498129752
1103	1	-0.5247873657119868
1103	2	-1.6612842375784105
1103	3	-0.46149920580665693
1103	4	0.016066446553026026
1103	5	-0.2708924587803665
1103	6	-0.11661025835351779
1103	7	-0.07952157583773811
1103	8	-0.0019457191901892656
1103	9	0.6497129148206255
1103	10	0.6801651065058962
1103	11	0.4504957911212415
1103	12	0.2065470406640415
1103	13	0.027883615520849503
1103	14	-0.9860080756626957
1103	15	-0.25653193790965645
1103	16	-0.14142569660432258
1103	17	0.3437485595689793
1103	18	0.46504207091201977
1103	19	0.4022534151445072
1103	20	0.4073893104867281
1103	21	0.5517604524115356
1103	22	0.10613615654848729
1103	23	0.9199646841316778
1103	24	0.8013360050144058
1103	25	0.6644531188114371
1104	1	-0.09779642265329969
1104	2	0.00450010300166781
1104	3	-0.4037190064774556
1104	4	-0.21240687507638256
1104	5	-0.26449396419535987
1104	6	-0.195699566732344
1104	7	-0.18248144160744317
1104	8	-0.1300729914672658
1104	9	-2.110967961916586
1104	10	-0.6181628899816047
1104	11	-0.6528688702045997
1104	12	-0.11105243546728616
1104	13	0.05144949848475022
1104	14	-1.9160684512183508
1104	15	-0.7064151916435151
1104	16	-0.3088462701229133
1104	17	0.05078625972004147
1104	18	0.28798278682313594
1104	19	0.15895735793281318
1104	20	0.34861019356006295
1104	21	0.44726779101672065
1104	22	0.533053259238109
1104	23	0.9861337241914043
1104	24	0.5886212185247146
1104	25	0.5019037712264733
1105	1	-0.37424852744860715
1105	2	-0.22729330704575545
1105	3	-1.6113779589041748
1105	4	-0.6368242388319914
1105	5	-0.58929105424032
1105	6	-0.3846976368439954
1105	7	-0.2825496246195769
1105	8	-0.30109397766890567
1105	9	0.1505133212990657
1105	10	0.26244390140124807
1105	11	-0.32635995547328633
1105	12	-0.3678721297262868
1105	13	-0.3983529038471403
1105	14	0.40057584123700707
1105	15	0.027314607631744536
1105	16	-0.38544011906988374
1105	17	0.10846750781153247
1105	18	-0.02261338553032033
1105	19	-0.01843629340956454
1105	20	-0.6179095772263636
1105	21	-0.12404951891603133
1105	22	0.04494528307319712
1105	23	0.1906515729903404
1105	24	0.10286143258527147
1105	25	0.4235044516427564
1106	1	-1.762970301365435
1106	2	-1.1207607007991989
1106	3	-1.2166129593991428
1106	4	-1.4649177130884894
1106	5	-1.0382551742205735
1106	6	-0.9465359966839273
1106	7	-0.7877546537376645
1106	8	-0.8835444155789602
1106	9	-0.4238745733012834
1106	10	-2.046691507882649
1106	11	-1.4333397638143808
1106	12	-0.9958795767176412
1106	13	-1.115597529192783
1106	14	-0.4355966651413965
1106	15	-1.185462096197
1106	16	-1.7519344275448958
1106	17	-0.9028431189394581
1106	18	-0.7301607065993778
1106	19	-0.69417560255566
1106	20	-0.35010333018326634
1106	21	-0.40938003305723925
1106	22	-0.4085251108545478
1106	23	-0.7214862880554307
1106	24	-0.9670379826236655
1106	25	-1.6117978219913596
1107	1	-0.06777226379479373
1107	2	0.11876067785335565
1107	3	-0.0382081921403703
1107	4	0.09161800410737442
1107	5	-0.24742256975726207
1107	6	-0.07173019202249264
1107	7	-0.15280589845882275
1107	8	-0.2141983282733264
1107	9	0.3783418968415049
1107	10	-0.05470960274404746
1107	11	-0.10496318380426573
1107	12	-0.13002590467569064
1107	13	-0.04669206290655836
1107	14	-1.12880970490302
1107	15	-0.5617660915937819
1107	16	-0.252068602578845
1107	17	0.0025224194329140914
1107	18	0.2117884241597281
1107	19	0.0686759992739212
1107	20	0.18733529289877993
1107	21	0.30935118054370747
1107	22	-0.30583924375218174
1107	23	0.379579424254878
1107	24	0.278478869282651
1107	25	-0.11812351620727668
1108	1	-0.1603026346933909
1108	2	0.09950246817841446
1108	3	-0.13805832559286912
1108	4	-0.287171461764558
1108	5	-0.3348797528473355
1108	6	-0.2672311572313859
1108	7	-0.13301379043553396
1108	8	-0.18916265815015976
1108	9	0.29347549757723357
1108	10	0.2946818552357868
1108	11	0.034366491774811436
1108	12	6.754002804498782E-4
1108	13	-0.14887066955016337
1108	14	0.15750856212684994
1108	15	-0.08026036572174888
1108	16	-0.10362807464335333
1108	17	0.2330784227534224
1108	18	0.2817372068302646
1108	19	0.26005399007655683
1108	20	0.351709694797151
1108	21	0.28894663805390053
1108	22	0.30774923739463134
1108	23	0.0914401255274413
1108	24	0.19770405451990314
1108	25	-0.6122779993041405
1109	1	-0.9438809923060845
1109	2	-0.7678760282236894
1109	3	-0.9058985644631505
1109	4	-0.8017152493770183
1109	5	-1.0024658351329947
1109	6	-1.321268734605873
1109	7	-0.955229758842485
1109	8	-1.4180366651893976
1109	9	-0.9749453794809853
1109	10	-1.2323286951116685
1109	11	-1.249764519019294
1109	12	-1.127616031927296
1109	13	-1.1619679060916113
1109	14	-0.593016106048033
1109	15	-1.3068955215414562
1109	16	-1.3995322053114307
1109	17	-1.47621692897755
1109	18	-2.04631642379848
1109	19	-2.196795968919325
1109	20	-1.899779340758407
1109	21	-1.7076692547529788
1109	22	-1.6578093818657038
1109	23	-1.1879842837252994
1109	24	-2.1302329212175053
1109	25	-1.2434590862255703
1110	1	0.12222213022574024
1110	2	0.2989916685478953
1110	3	0.09685151142964443
1110	4	0.30131594538482176
1110	5	0.1874920036793455
1110	6	0.2279011609643078
1110	7	0.1964757864132025
1110	8	0.09492190498369979
1110	9	0.43038956847017573
1110	10	0.4251835623220046
1110	11	0.2908349709545561
1110	12	0.34722243432747746
1110	13	0.32670065197186204
1110	14	-2.4512669647824836
1110	15	-0.5633676657304534
1110	16	-0.03183709440166638
1110	17	-0.11115896813890203
1110	18	-2.6703033297173207
1110	19	-0.41827605445067206
1110	20	-1.8258321913714965
1110	21	-0.19897562715709682
1110	22	-1.7130284479957254
1110	23	-0.47036493275649605
1110	24	0.2184255532343613
1110	25	0.3077939586199321
1111	1	-0.17511725316654392
1111	2	-1.3931971114114106
1111	3	-0.3625298549424478
1111	4	-0.10518214258011938
1111	5	-0.1389359197083806
1111	6	-0.04481644548740604
1111	7	-0.07844987763991185
1111	8	-0.13549688039901042
1111	9	-1.8578328869628606
1111	10	-0.43930354795693205
1111	11	-0.1767796599201219
1111	12	-0.1454738937347031
1111	13	-0.12821961383813374
1111	14	0.828330255814393
1111	15	0.1965354049764585
1111	16	0.2930157122443333
1111	17	-0.11464706369157796
1111	18	0.03435363017839421
1111	19	-0.1964356310472155
1111	20	0.31624824622594616
1111	21	-0.6046542265835555
1111	22	-0.2953249159843949
1111	23	-0.020076900623735128
1111	24	-0.5930436371424221
1111	25	-2.122744557026799
1112	1	0.23338631317631453
1112	2	0.4400821995320463
1112	3	0.08444673682919301
1112	4	0.3337434789705469
1112	5	0.06649714664668238
1112	6	0.11868500898373213
1112	7	0.05905022569473317
1112	8	0.04811099625768378
1112	9	-0.35730655316255167
1112	10	0.4215813964644113
1112	11	0.13168919559536363
1112	12	0.03588345053230313
1112	13	0.06241242313086559
1112	14	0.47379943065795516
1112	15	0.15950281640233044
1112	16	0.27100966289017236
1112	17	-3.614259198312297
1112	18	-0.9069236475237972
1112	19	-1.1950858883648934
1112	20	-0.17377986464183692
1112	21	-0.6091829754346136
1112	22	-1.7624347488860295
1112	23	-1.54043493064826
1112	24	-0.4422141819822585
1112	25	-0.08323539754020715
1113	1	-0.3089856964616402
1113	2	-0.10316919200475994
1113	3	-0.14324772974620004
1113	4	-0.6302008251124178
1113	5	-0.25125412798993807
1113	6	-0.293460671996975
1113	7	-0.3812538893567492
1113	8	-0.2893700561223298
1113	9	0.242438507716372
1113	10	0.3451340106284704
1113	11	-0.0892851974850899
1113	12	-0.07615219564730324
1113	13	-0.4289633634091672
1113	14	-1.6394821048465502
1113	15	-1.7952948448391126
1113	16	-0.6804042158861137
1113	17	-0.4314051871733966
1113	18	-0.6298953704201224
1113	19	-0.22550701919895744
1113	20	0.18477455079946026
1113	21	-1.6495620906217567
1113	22	-1.9608143071378308
1113	23	-0.8218385302301101
1113	24	-2.4381126725190043
1113	25	-0.7679150804304768
1114	1	0.11181453394111429
1114	2	0.23607630490950626
1114	3	-0.03689507553425031
1114	4	0.212725346393988
1114	5	-0.029166053569660733
1114	6	0.14243889839985258
1114	7	0.08092169760627854
1114	8	0.062187081712160364
1114	9	0.33535767341537337
1114	10	0.23782263227883504
1114	11	0.18469151309169973
1114	12	0.24552603315271426
1114	13	0.10433463867585147
1114	14	0.835049465676498
1114	15	0.42711931164872396
1114	16	0.32880624257157365
1114	17	-0.550938367265978
1114	18	-0.2069974482130803
1114	19	-0.5634900342959455
1114	20	-1.7773429173982933
1114	21	-0.7734603479301043
1114	22	-1.4428464952316276
1114	23	-2.9440033977190936
1114	24	-2.252847027609114
1114	25	-1.3660293290181897
1115	1	0.4138574635566421
1115	2	0.46849642563596916
1115	3	0.3475624978223877
1115	4	0.4766837509664583
1115	5	0.4370864240974228
1115	6	0.3506939214806266
1115	7	0.4154010991196131
1115	8	0.5075778770539756
1115	9	0.6503045119276578
1115	10	0.8571928474844697
1115	11	0.5660601981205531
1115	12	0.46407774176094563
1115	13	0.43005757492947083
1115	14	0.9799696063491368
1115	15	0.6386296914980747
1115	16	0.5692020178869512
1115	17	0.5297094676446363
1115	18	0.7642781336966706
1115	19	0.16868153360773874
1115	20	0.7641118543888155
1115	21	-0.3640050061986031
1115	22	0.7266857720966939
1115	23	0.7941896157501631
1115	24	0.7562848950868286
1115	25	0.8323857525062905
1116	1	0.43165710598017526
1116	2	0.4890951055604411
1116	3	0.33933817350781226
1116	4	0.5660470478044317
1116	5	0.5225998558380596
1116	6	0.2834848900682587
1116	7	0.2842576511526289
1116	8	0.32667602107276394
1116	9	0.4932093928497139
1116	10	0.44331759531043957
1116	11	0.3560515652685652
1116	12	0.35977793884440246
1116	13	-0.19282892552610775
1116	14	0.8795781935614286
1116	15	0.4504626987633742
1116	16	0.48197726176584155
1116	17	0.6222937723787396
1116	18	0.5389495492516048
1116	19	0.6282890978615043
1116	20	0.5377421145514347
1116	21	0.021948068201631088
1116	22	0.19913195775645676
1116	23	-0.6577151035313307
1116	24	-0.22676251805760472
1116	25	-1.0480221981110418
1201	1	0.2635738912329053
1201	2	0.5740398989588655
1201	3	0.4691400174883924
1201	4	0.32612260094328605
1201	5	0.37296892770432777
1201	6	0.29820326365246785
1201	7	0.3227245732925468
1201	8	0.33228366835877665
1201	9	0.6670574252247974
1201	10	0.9360346733811582
1201	11	0.5610615305032667
1201	12	0.6229556339719906
1201	13	0.4582621969043643
1201	14	1.0541315995917273
1201	15	0.7162654194784679
1201	16	0.4654294107484773
1201	17	0.542532596071446
1201	18	0.5599610868247566
1201	19	0.8151390131788077
1201	20	0.7669560681807894
1201	21	0.7231680055879934
1201	22	0.7480125813009053
1201	23	0.3603579470453582
1201	24	0.7729192577957988
1201	25	0.9155609329952341
1202	1	0.40514476477139283
1202	2	0.7350169250653948
1202	3	0.5721665496489088
1202	4	0.4982813179542731
1202	5	0.3694786978293152
1202	6	0.2543473244810883
1202	7	0.24914131965898179
1202	8	0.2924355037051285
1202	9	0.8175535432933714
1202	10	0.0323264755156103
1202	11	0.3885505093158983
1202	12	0.44259848914342115
1202	13	0.3422160972789996
1202	14	1.020637721640604
1202	15	0.7553767287275122
1202	16	0.655654692606582
1202	17	-1.436855324588585
1202	18	0.1863236114166682
1202	19	0.5960464067063427
1202	20	0.8185886303808161
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2210	23	1.603124931550262
2210	24	1.1846494734230717
2210	25	1.2863289195889678
2211	1	0.11685626856760223
2211	2	-0.6234119063310892
2211	3	-0.07639183944842287
2211	4	0.24426995117337214
2211	5	0.2550875346257513
2211	6	0.12630370065961927
2211	7	0.11021175687934459
2211	8	0.31070876727230967
2211	9	0.7464675821528388
2211	10	0.7213535622729239
2211	11	0.41037915018306137
2211	12	0.17515304972909007
2211	13	0.17604726409746974
2211	14	-0.9530082112379503
2211	15	0.032662175043022046
2211	16	0.136729663394199
2211	17	0.24938178844773518
2211	18	0.2300977800168198
2211	19	0.4494434639207867
2211	20	0.6081353595464805
2211	21	0.6121776390401195
2211	22	-0.1926632321303643
2211	23	0.5407049250242579
2211	24	-0.17376999018500072
2211	25	0.29748495126507635
2212	1	0.03090399626393392
2212	2	-1.131661883657685
2212	3	-0.3316909534360235
2212	4	0.10503681929413489
2212	5	0.19469379577053303
2212	6	0.06678926144969921
2212	7	0.00984962510793963
2212	8	0.14006257879668738
2212	9	-2.176357074229024
2212	10	0.036154047019614666
2212	11	0.05551507992478122
2212	12	-0.044700355102236244
2212	13	0.12409817249762511
2212	14	0.6047496996380701
2212	15	0.33280492822997587
2212	16	0.29899590415791266
2212	17	0.346526153410295
2212	18	0.1535442947038277
2212	19	-0.4315937140365236
2212	20	0.35423121341978003
2212	21	0.33056873421200805
2212	22	-1.5578668036022947
2212	23	-0.37433671905827026
2212	24	0.2761304253631707
2212	25	-0.36714317989646617
2213	1	0.23542853645759365
2213	2	0.42808682538894516
2213	3	0.14736191418725397
2213	4	0.32318967141662824
2213	5	0.3484130184326446
2213	6	0.26122316735411744
2213	7	0.14290512980822
2213	8	0.2699281421149635
2213	9	0.6215515910303822
2213	10	0.6901724504163411
2213	11	0.09138933705254715
2213	12	0.01372770536210055
2213	13	0.08770725425815584
2213	14	-1.722670244324874
2213	15	-0.38204015001578284
2213	16	-0.05912400418026209
2213	17	-2.1456344056686234
2213	18	-0.8590725781791875
2213	19	-0.47234320172596966
2213	20	0.20967876612895964
2213	21	-0.16049249747944572
2213	22	0.3095733705640953
2213	23	0.9255293407212619
2213	24	0.6413062478043514
2213	25	-0.9478458494402418
2214	1	0.45508217596924183
2214	2	0.3401513182237892
2214	3	0.2171134520877125
2214	4	0.5091159842052662
2214	5	0.666459084076223
2214	6	0.5025228576339842
2214	7	0.4241590686946992
2214	8	0.3326560492551849
2214	9	0.6688708867392634
2214	10	0.715601883811182
2214	11	0.14241252136339
2214	12	0.1397045580509939
2214	13	0.28375946921612893
2214	14	1.0866744327342488
2214	15	0.6902665866512194
2214	16	0.634795938616108
2214	17	0.5383325126489495
2214	18	0.5532811092481893
2214	19	-0.7047416364365091
2214	20	0.25939724385352037
2214	21	0.605846902671523
2214	22	0.7222846042394337
2214	23	1.2187823732202148
2214	24	0.9815060081997871
2214	25	1.0217601019102176
2215	1	-0.019568192652979377
2215	2	0.3378145166583728
2215	3	0.24045107763668483
2215	4	0.5287439702889287
2215	5	0.4398572042590203
2215	6	0.3831017146499889
2215	7	0.21207014186855133
2215	8	0.2830127058162546
2215	9	0.3814978397973334
2215	10	0.7321474755372321
2215	11	0.3601469873314258
2215	12	0.27086315927417814
2215	13	0.2140704601820076
2215	14	1.1335417102682044
2215	15	0.5389856187294626
2215	16	0.5324998697868324
2215	17	0.7552518003372484
2215	18	0.5608571935062159
2215	19	-0.2204913025023174
2215	20	-1.4189372241706621
2215	21	-1.7166633647576006
2215	22	-0.014010743413855664
2215	23	0.6593213123451918
2215	24	0.7293001434853963
2215	25	0.04729116959338597
2216	1	0.16945423276685645
2216	2	-0.18972865764532898
2216	3	0.022667381910061124
2216	4	0.2529493280433357
2216	5	0.35679142396151065
2216	6	0.18715112498255593
2216	7	0.10437196971305696
2216	8	0.10355447444494814
2216	9	0.3856594083625211
2216	10	-1.5250665326446367
2216	11	-0.34382026962534695
2216	12	-0.06279902106318502
2216	13	0.015265128268360684
2216	14	-0.38007469612286826
2216	15	-0.4278846995788754
2216	16	0.0951927025566448
2216	17	0.37175626175979914
2216	18	0.24191393265154204
2216	19	-1.2823116204993212
2216	20	-0.9744325884008234
2216	21	-0.8514828530483101
2216	22	-0.616584350659231
2216	23	-4.535528320902834
2216	24	-1.993184702310657
2216	25	-1.2443143976891022
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	10.0
4	18.0
5	54.0
6	74.0
7	250.0
8	149.0
9	427.0
10	1237.0
11	2734.0
12	5521.0
13	9214.0
14	13183.0
15	17207.0
16	20156.0
17	22404.0
18	23922.0
19	24817.0
20	25773.0
21	26757.0
22	28585.0
23	30800.0
24	33427.0
25	36855.0
26	41691.0
27	48349.0
28	56590.0
29	69245.0
30	85328.0
31	105876.0
32	138697.0
33	184516.0
34	247550.0
35	340315.0
36	478643.0
37	708771.0
38	1163509.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.48744309091141	21.655194402674837	13.894828820920639	22.96253368549311
2	15.313687477125661	21.418971677531534	41.08167024431107	22.185670601031735
3	17.74619820422005	26.824134172155667	30.46146669308969	24.968200930534596
4	12.195394500138805	17.527187121357134	39.44577959465107	30.831638783852988
5	13.16478025874317	37.699150302067494	35.57821305342498	13.557856385764364
6	30.15111743031983	38.984850181397526	18.139652675647994	12.72437971263465
7	25.288340499938002	33.32194358819558	24.329873405988884	17.059842505877533
8	22.851997336183558	37.85563277416239	21.710504887421685	17.581865002232373
9	23.99234529935687	17.61718629620174	22.7492581940649	35.64121021037649
10	14.712186421231419	30.16330896683718	34.10381730639157	21.020687305539827
11	31.460851484900488	26.263261395092503	24.12042035647632	18.155466763530693
12	20.471088461787033	27.726640337829412	32.17598170775391	19.626289492629645
13	25.706018663493456	24.704803657543685	27.61525646646208	21.973921212500784
14	20.553641332780114	23.55423564383029	28.652682818297166	27.239440205092436
15	22.195360947302987	31.257796666690886	24.690394506636828	21.856447879369295
16	21.603852709612838	31.729816312890364	26.01307985293808	20.653251124558718
17	19.768299724810518	30.717534342319684	29.054047114142882	20.46011881872691
18	19.854265451226702	30.508730619812752	29.795515177842898	19.841488751117648
19	20.559734431252625	29.19507739910161	29.91729032503715	20.327897844608618
20	21.791203671232328	28.937707283348196	29.149299119264953	20.121789926154523
21	22.224312371501743	29.386324378699648	28.14354833538396	20.24581491441465
22	20.972754019636948	28.94198721682179	29.82230555628619	20.262953207255073
23	21.310920193269244	29.244899227043263	29.09174076590253	20.35243981378496
24	20.952797500596194	28.290963508944905	29.44134156581476	21.314897424644137
25	21.696530499823357	28.686204466148098	28.721207120181713	20.896057913846835
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2828.0
1	2828.0
2	3220.5
3	3613.0
4	3613.0
5	3613.0
6	5480.0
7	7347.0
8	7347.0
9	7347.0
10	12334.5
11	17322.0
12	17322.0
13	17322.0
14	29164.0
15	41006.0
16	41006.0
17	41006.0
18	63965.0
19	86924.0
20	86924.0
21	86924.0
22	123864.5
23	160805.0
24	160805.0
25	160805.0
26	208506.5
27	256208.0
28	256208.0
29	256208.0
30	306437.0
31	356666.0
32	356666.0
33	356666.0
34	399694.5
35	442723.0
36	442723.0
37	442723.0
38	471574.0
39	500425.0
40	500425.0
41	500425.0
42	498115.0
43	495805.0
44	495805.0
45	495805.0
46	469683.0
47	443561.0
48	443561.0
49	443561.0
50	414625.5
51	385690.0
52	385690.0
53	385690.0
54	346777.0
55	307864.0
56	307864.0
57	307864.0
58	263391.5
59	218919.0
60	218919.0
61	218919.0
62	178686.0
63	138453.0
64	138453.0
65	138453.0
66	105896.0
67	73339.0
68	73339.0
69	73339.0
70	53114.5
71	32890.0
72	32890.0
73	32890.0
74	22956.0
75	13022.0
76	13022.0
77	13022.0
78	8993.0
79	4964.0
80	4964.0
81	4964.0
82	3315.0
83	1666.0
84	1666.0
85	1666.0
86	1075.0
87	484.0
88	484.0
89	484.0
90	291.0
91	98.0
92	98.0
93	98.0
94	58.5
95	19.0
96	19.0
97	19.0
98	16.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07957113238462436
2	0.01840880777547967
3	0.029679506413528445
4	0.0357155916841279
5	0.04568389847955771
6	0.014852276205250944
7	0.01900991170284227
8	0.03476384379913711
9	0.03458852182032302
10	0.0208883614758504
11	0.020136981566647148
12	0.014526678244596202
13	0.02264158126399132
14	0.037017983526746866
15	0.034187785868747954
16	0.021689833379000534
17	0.021514511400186443
18	0.025371594934096468
19	0.015478426129586985
20	0.013374562383817882
21	0.009567570843854739
22	0.01725669191470135
23	0.017782657851143625
24	0.016580449996418424
25	0.10982669672854196
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3992654.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.64581049280999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.16397158697862	69.42245162817173
2	9.720830829948053	15.48446900229415
3	1.7343861598669956	4.144097742303575
4	0.5347473001294908	1.703615285106208
5	0.25830358715651136	1.0286399276140266
6	0.14007021810759154	0.6693603628250279
7	0.08785409329921724	0.4898047326148973
8	0.06529960324747942	0.41606718604035403
9	0.05030562672675487	0.36059691717010395
>10	0.22051975298885843	3.084000577922632
>50	0.014789515433172402	0.8172452984846413
>100	0.007816307253071532	1.091546927636008
>500	6.948347579709254E-4	0.38169973169915555
>1k	4.105841062795101E-4	0.906404680117585
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4922	0.12327639710328017	No Hit
GTGGTATCAACGCAGAGTACATGGG	4811	0.12049629143922815	No Hit
ATTCCATTCCATTCCATTCCATTCC	4344	0.10879981085263085	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.009199394688345E-5	0.0
5	0.0	0.0	0.0	5.009199394688345E-5	0.0
6	0.0	0.0	0.0	5.009199394688345E-5	0.0
7	0.0	0.0	0.0	5.009199394688345E-5	0.0
8	2.5045996973441725E-5	0.0	0.0	7.513799092032517E-5	0.0
9	2.5045996973441725E-5	0.0	0.0	1.5027598184065035E-4	0.0
10	2.5045996973441725E-5	0.0	0.0	1.5027598184065035E-4	0.0
11	2.5045996973441725E-5	0.0	0.0	1.5027598184065035E-4	0.0
12	2.5045996973441725E-5	0.0	0.0	1.5027598184065035E-4	0.0
13	2.5045996973441725E-5	0.0	0.0	1.7532197881409207E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGACGG	110	3.8086E-8	12.093509	5
GTCTAGC	200	2.5465852E-11	9.983525	1
GTATAGC	395	0.0	9.147052	1
CCTACAC	390	0.0	9.014532	3
ATAACGC	95	0.0018216114	9.00171	3
TCAGGAC	435	0.0	8.955724	3
GTATTAG	590	0.0	8.863502	1
GTATAGG	570	0.0	8.840883	1
GGTATCA	3315	0.0	8.83409	1
TAGCACT	495	0.0	8.830181	4
TACACCG	120	1.7609047E-4	8.710205	5
GTCTACG	110	7.088015E-4	8.643745	1
GTGTAGG	550	0.0	8.643745	1
GTCTTAA	550	0.0	8.643745	1
CTAGAAC	430	0.0	8.617915	3
GTATTAT	800	0.0	8.557307	1
TGAACCG	100	0.002900818	8.551838	5
CTACACT	550	0.0	8.465456	4
TATACTG	1060	0.0	8.42634	5
GACTCCG	205	3.875175E-8	8.34033	7
>>END_MODULE