FastQCFastQC Report
Wed 25 May 2016
SRR1295295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295295_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3992654
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT125770.3150035039349766No Hit
GGTATCAACGCAGAGTACTTTTTTT98430.2465277482095869No Hit
TATCAACGCAGAGTACTTTTTTTTT75420.1888969091736975No Hit
GTGGTATCAACGCAGAGTACATGGG53380.13369553184423194No Hit
ACGCAGAGTACTTTTTTTTTTTTTT47230.11829224370556526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA3100.012.5747573
CAGCGTC3500.010.8659912
CTATGCG700.001497444510.8526569
ACCGATC700.001497444510.8526568
GCAGCGT3550.010.7263981
ACACCGC856.6228915E-410.05466656
GGTATCA39900.010.0207141
GGACTGT4350.09.1686236
TACACCG1252.7304557E-59.1273185
CTAGACA4950.09.0275534
ATAGGCG950.00181149329.0072225
TGAACCG850.00739023978.9483515
GTATTAG6200.08.9055061
CTTACAC5100.08.7620383
TTAGGGT3800.08.7571314
CTAGGAC3800.08.7571313
GCGTCAG4550.08.5674164
AGGACCG1000.0028854418.5568615
TATCCCG1000.0028854418.5568615
CTGTCGC3000.08.5464679
Produced by FastQC (version 0.11.5)