FastQCFastQC Report
Wed 25 May 2016
SRR1295294_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295294_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4059686
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA77010.18969447390758792No Hit
GTGGTATCAACGCAGAGTACATGGG58640.14444466887340549No Hit
GTATCAACGCAGAGTACTTTTTTTT54640.13459169009622912No Hit
GCTTATGTACTCTGCGTTGATACCA52500.12932034645043974No Hit
GGTATCAACGCAGAGTACTTTTTTT44790.11032872985743232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG550.003072745912.08941910
TCTGTCG2053.6379788E-1210.1941178
GTGCTAG3550.010.177671
GTGTTAG6050.09.5866651
TGGACCG1002.7518396E-49.5018745
TCTAGAC3000.09.1850313
GTCGCCC2304.1836756E-119.08538911
GTCTAAG2850.09.007661
ACATAAG22450.08.8034093
TGTCGCC2701.8189894E-128.79521210
TTAGACA4650.08.7866794
GTCCTAT2950.08.7023151
TACACTG7350.08.66157155
CATAAGC22350.08.5878244
GTCTTAG4100.08.5804671
TAATACG1000.0029013068.5516874
ACTGACG1000.0029090858.5491578
TAGACTG4450.08.541015
GTCTTGC7600.08.5072351
GTATTAG6600.08.4996531
Produced by FastQC (version 0.11.5)