##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295294_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4059686
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.148373544160805	31.0	30.0	33.0	26.0	34.0
2	30.026451553149677	31.0	30.0	34.0	25.0	34.0
3	30.475828179814894	31.0	30.0	34.0	26.0	34.0
4	33.88074250077469	35.0	35.0	37.0	28.0	37.0
5	34.199965218984914	35.0	35.0	37.0	30.0	37.0
6	34.28750819644672	37.0	35.0	37.0	30.0	37.0
7	34.262622527949205	37.0	35.0	37.0	30.0	37.0
8	34.1982402087255	37.0	35.0	37.0	30.0	37.0
9	35.27295928798434	39.0	35.0	39.0	28.0	39.0
10	35.21355789585697	39.0	35.0	39.0	27.0	39.0
11	35.575769160472014	39.0	35.0	39.0	29.0	39.0
12	35.58491617331981	39.0	35.0	39.0	29.0	39.0
13	35.49243980938427	39.0	35.0	39.0	28.0	39.0
14	35.73510389719796	39.0	34.0	41.0	25.0	41.0
15	36.328790453251806	39.0	36.0	41.0	27.0	41.0
16	36.424674469897425	39.0	36.0	41.0	27.0	41.0
17	36.257296253946734	39.0	36.0	41.0	27.0	41.0
18	36.236331332029124	39.0	36.0	41.0	27.0	41.0
19	36.297465124149994	39.0	36.0	41.0	27.0	41.0
20	36.26631493174595	39.0	36.0	41.0	27.0	41.0
21	36.26393987121171	39.0	35.0	41.0	27.0	41.0
22	36.018074796917794	39.0	35.0	41.0	27.0	41.0
23	35.70632605575899	39.0	34.0	41.0	26.0	41.0
24	36.01711708738065	39.0	34.0	41.0	27.0	41.0
25	34.7603100338302	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.317645876028962
1101	2	0.5287627936831107
1101	3	0.4459372908805612
1101	4	0.37007118483868595
1101	5	0.348086907090952
1101	6	0.26398476310715324
1101	7	0.26259954510469186
1101	8	0.20112287286052322
1101	9	0.324895130100856
1101	10	0.6822687129590577
1101	11	0.5056818037518411
1101	12	0.4730046968722519
1101	13	0.4586726519103692
1101	14	0.9953921106235768
1101	15	0.735512086354305
1101	16	0.5592038549395326
1101	17	0.821284953593505
1101	18	0.7215544204425868
1101	19	0.6960437982897432
1101	20	0.8135444268569003
1101	21	0.8303979042786622
1101	22	0.6470474659350742
1101	23	-1.4815045347646674
1101	24	0.1522727041569354
1101	25	0.5729204697477357
1102	1	0.31643155704566084
1102	2	0.21577900678223116
1102	3	0.27332378809705205
1102	4	0.4359604655626157
1102	5	0.23150119502317779
1102	6	0.229307361638881
1102	7	0.1795711777260678
1102	8	0.2962722507299702
1102	9	0.38255864730093947
1102	10	0.920128295543833
1102	11	0.46902489599572306
1102	12	0.4056737589078736
1102	13	0.42341858754592465
1102	14	0.9925531548270072
1102	15	0.6603740199493586
1102	16	0.49753419406167154
1102	17	0.8375285493084519
1102	18	0.8536185409207704
1102	19	0.7296981776008522
1102	20	0.9091457088228765
1102	21	0.7696043274161539
1102	22	0.738625969716658
1102	23	0.8887878998617254
1102	24	0.8611743831331324
1102	25	0.6492451309582847
1103	1	-0.3338065405786601
1103	2	-1.4886026767383491
1103	3	-0.2789769751611786
1103	4	0.005939762666358206
1103	5	-0.045002019939033744
1103	6	0.009712771881233095
1103	7	0.0706926991455461
1103	8	0.12160194363701038
1103	9	0.7352844212613903
1103	10	0.777316789257803
1103	11	0.47275635680983896
1103	12	0.3118081015193326
1103	13	0.116283390188336
1103	14	-0.8730183295821092
1103	15	-0.12465339062801917
1103	16	0.0033928559902491884
1103	17	0.4572536775561673
1103	18	0.6254226207944384
1103	19	0.6250066307108
1103	20	0.6629099205880209
1103	21	0.682362952549127
1103	22	0.2185952733061569
1103	23	0.9989526138797018
1103	24	0.8457778568099599
1103	25	0.7452863105365779
1104	1	-0.16796783635818002
1104	2	-0.06891251283628108
1104	3	-0.4349101997393099
1104	4	-0.3581107345261785
1104	5	-0.28225389177860194
1104	6	-0.18888144141485697
1104	7	-0.07416490969989553
1104	8	-0.022875458605525978
1104	9	-2.189503296718655
1104	10	-0.6192909044930701
1104	11	-0.5160685944836843
1104	12	-0.04990655093475738
1104	13	0.07376592438986052
1104	14	-1.7793937910737867
1104	15	-0.5294961269570493
1104	16	-0.20194195364910428
1104	17	0.03904320174817144
1104	18	0.24946451768041555
1104	19	0.24106843190129723
1104	20	0.35722122917945853
1104	21	0.47988402763541416
1104	22	0.5865819511324375
1104	23	1.0442658249799663
1104	24	0.6687397529506498
1104	25	0.5414024106166977
1105	1	-0.2758389111187931
1105	2	-0.11921663715351372
1105	3	-1.4063611419105406
1105	4	-0.5708069803037219
1105	5	-0.3955907885127061
1105	6	-0.2055525322858216
1105	7	-0.13085099879703677
1105	8	-0.10573288617150212
1105	9	0.2167517669788026
1105	10	0.39503897705283464
1105	11	-0.21651550989452772
1105	12	-0.21974052322040905
1105	13	-0.28840905450602605
1105	14	0.4781493303622
1105	15	0.16866157988798847
1105	16	-0.21302487253210955
1105	17	0.23062668522802454
1105	18	0.11554763273475288
1105	19	0.18856614708622033
1105	20	-0.406465526900206
1105	21	-0.02397253509410291
1105	22	0.15492268702891465
1105	23	0.3078501686757633
1105	24	0.19577033252194553
1105	25	0.4505980811942649
1106	1	-1.6841554994677743
1106	2	-1.1594294554390352
1106	3	-1.2623958772874602
1106	4	-1.608456333354276
1106	5	-1.020359498416319
1106	6	-0.8607866480983191
1106	7	-0.683435744116295
1106	8	-0.8523097578595653
1106	9	-0.5359037084566509
1106	10	-1.9960835648357858
1106	11	-1.3884748126054731
1106	12	-1.0722021231338914
1106	13	-1.1121148067570061
1106	14	-0.4966854873442301
1106	15	-1.1716953150337446
1106	16	-1.5868965919978066
1106	17	-0.7850597893597211
1106	18	-0.7865229892367651
1106	19	-0.8204896625512106
1106	20	-0.5312009212620339
1106	21	-0.5149766411038073
1106	22	-0.4777320383707817
1106	23	-0.7760761289733082
1106	24	-1.1281911295942706
1106	25	-1.7890939855205445
1107	1	-0.039408644366048406
1107	2	0.11209950868583007
1107	3	-0.049354255917680945
1107	4	0.15008277727366703
1107	5	-0.06982758352270224
1107	6	0.027468515746996047
1107	7	-0.08139220733522023
1107	8	-0.1556436088716282
1107	9	0.3798984347332848
1107	10	-0.04691702758880467
1107	11	0.06603934590775395
1107	12	-0.0683674179519258
1107	13	0.03902279224904959
1107	14	-0.9803048381751012
1107	15	-0.501460547730332
1107	16	-0.18346697042095883
1107	17	0.023822283425907642
1107	18	0.29274656607721283
1107	19	0.2838678981466174
1107	20	0.3367897087529812
1107	21	0.49217767892518083
1107	22	-0.0823613804011174
1107	23	0.5732288873335705
1107	24	0.40578024277203895
1107	25	0.10540567798827993
1108	1	-0.07070788265903616
1108	2	0.20823548084710808
1108	3	-0.15931041667025525
1108	4	-0.27773499439184235
1108	5	-0.2319090638353316
1108	6	-0.13180247914791465
1108	7	-0.14849169165527343
1108	8	-0.20235704276156952
1108	9	0.32477387721167617
1108	10	0.3043119430448513
1108	11	0.06451277738880634
1108	12	0.002184192905296811
1108	13	-0.17165699122286782
1108	14	0.10081820796280994
1108	15	-0.053810807199262456
1108	16	-0.07546121344486778
1108	17	0.2760385567958181
1108	18	0.21297008011693208
1108	19	0.3358275949888778
1108	20	0.37467069888724325
1108	21	0.4124173650384009
1108	22	0.35343093942946524
1108	23	0.12322288979788709
1108	24	0.25037949773307844
1108	25	-0.4956124856713586
1109	1	-0.8015152673594557
1109	2	-0.690135451570562
1109	3	-0.783366219002481
1109	4	-0.7168702598533088
1109	5	-1.0374291309114838
1109	6	-1.3602869704709875
1109	7	-0.9569528298674186
1109	8	-1.2252949287170765
1109	9	-1.038708350622997
1109	10	-1.114619992946686
1109	11	-1.152949345165112
1109	12	-1.19343036297915
1109	13	-1.2576177081109705
1109	14	-0.6849350037852346
1109	15	-1.296598603234905
1109	16	-1.2778056829069016
1109	17	-1.3564492155363794
1109	18	-1.8714681858840834
1109	19	-2.091363292467399
1109	20	-1.8790893803206998
1109	21	-1.6469237719871117
1109	22	-1.7091150170941845
1109	23	-1.2205796750725995
1109	24	-2.186588937460165
1109	25	-1.1814859906785102
1110	1	0.2202159736963054
1110	2	0.2902788069887059
1110	3	0.10819256534704635
1110	4	0.38349830018267284
1110	5	0.32833559278548563
1110	6	0.24615070578697384
1110	7	0.24597286226504878
1110	8	0.1701882174374134
1110	9	0.5366395152640138
1110	10	0.35470631525588914
1110	11	0.3800209724054966
1110	12	0.35564934727593567
1110	13	0.376843512231261
1110	14	-2.1631371420266206
1110	15	-0.4506794715277138
1110	16	0.004168975591205992
1110	17	0.017415512542356737
1110	18	-2.5385394950747937
1110	19	-0.17989780989056925
1110	20	-1.6062787944549655
1110	21	-0.03811793094619276
1110	22	-1.5386628935331998
1110	23	-0.3137258504331939
1110	24	0.18308777775466467
1110	25	0.1854749248777381
1111	1	-0.1842188713421109
1111	2	-1.331554416598209
1111	3	-0.375610344064242
1111	4	-0.030056193799872233
1111	5	-0.10657182281489952
1111	6	-0.006161318973425978
1111	7	-0.07132626477415727
1111	8	-0.041610404934104395
1111	9	-1.7466376774167003
1111	10	-0.28048863240519495
1111	11	-0.20985253078188748
1111	12	-0.09138553905508218
1111	13	0.011787837689979597
1111	14	0.8428025496813021
1111	15	0.19680913598865857
1111	16	0.1939214912006051
1111	17	-0.1277406785829598
1111	18	0.10572056591175993
1111	19	-0.05921832735351984
1111	20	0.26878749463190843
1111	21	-0.5064812665692173
1111	22	-0.18873516744225327
1111	23	-0.10405828672430317
1111	24	-0.5517582438162805
1111	25	-1.816551042557954
1112	1	0.11945299404915488
1112	2	0.43195009901021564
1112	3	0.025311143133066594
1112	4	0.3539471119923334
1112	5	0.1496947302330014
1112	6	0.12327681292092763
1112	7	0.09950923043213322
1112	8	0.028545257055782258
1112	9	-0.33376254933985194
1112	10	0.440916705155054
1112	11	0.21978065141659897
1112	12	0.11458594063662986
1112	13	0.07726088879867632
1112	14	0.3690199131312184
1112	15	0.0556298988187649
1112	16	0.15694498264149104
1112	17	-3.720721793745426
1112	18	-1.0972347114491043
1112	19	-1.212187937902364
1112	20	-0.17761185391577783
1112	21	-0.6208710022461972
1112	22	-1.807460829272749
1112	23	-1.644534448313216
1112	24	-0.4473122435369916
1112	25	-0.10087381786281213
1113	1	-0.2289862377547216
1113	2	0.022418446782445756
1113	3	-0.02801386889506219
1113	4	-0.31458077134412576
1113	5	-0.2056578591352647
1113	6	-0.22499219762054423
1113	7	-0.2937064193424348
1113	8	-0.2104818513112221
1113	9	0.28494523478219236
1113	10	0.39376705131368794
1113	11	0.02608797080936398
1113	12	-0.05274254181136939
1113	13	-0.38588293620301783
1113	14	-1.4775171124237474
1113	15	-1.6876312881814926
1113	16	-0.4842575272855143
1113	17	-0.22001328629985295
1113	18	-0.6102959917579085
1113	19	-0.11654209683966599
1113	20	0.20633187828214972
1113	21	-1.5134705931356152
1113	22	-1.9518573481591304
1113	23	-0.6300668562074776
1113	24	-2.3085127418431455
1113	25	-0.5511869230959192
1114	1	0.14847156461951982
1114	2	0.27393955347717736
1114	3	0.08018869798533856
1114	4	0.33621378821094083
1114	5	0.0902039612032155
1114	6	0.20519896364404389
1114	7	0.15085439225563846
1114	8	0.17419961435746956
1114	9	0.5219374091279221
1114	10	0.34795405278634206
1114	11	0.23775183732317373
1114	12	0.2716630177980761
1114	13	0.24877858494479455
1114	14	0.9744116575361943
1114	15	0.5034007139788201
1114	16	0.4540380546261531
1114	17	-0.2739721937330657
1114	18	0.023358342508608132
1114	19	-0.34842632632091153
1114	20	-1.5173032699492737
1114	21	-0.70559262161904
1114	22	-1.3343336306283788
1114	23	-2.9804771010658797
1114	24	-1.8603310744446446
1114	25	-1.2754616210609342
1115	1	0.29104067228863784
1115	2	0.46154741558929047
1115	3	0.2479246866581022
1115	4	0.5248049631373561
1115	5	0.4280820616958536
1115	6	0.2965135139845714
1115	7	0.3846148446133739
1115	8	0.3668869884764163
1115	9	0.5304499409811996
1115	10	0.7596358602416089
1115	11	0.4732689077543668
1115	12	0.4415380468387511
1115	13	0.3584575002228476
1115	14	1.0509932540692404
1115	15	0.5968095774684201
1115	16	0.5549756798212044
1115	17	0.46668045779525613
1115	18	0.7886954796373189
1115	19	0.06999397050683598
1115	20	0.6463085764525118
1115	21	-0.40244630416005833
1115	22	0.6311861655106341
1115	23	0.7847448337476237
1115	24	0.8145586624422307
1115	25	0.8781166021800217
1116	1	0.3613918450181757
1116	2	0.4282699111355939
1116	3	0.2622630475135814
1116	4	0.44916155414505
1116	5	0.28256806370809073
1116	6	0.2252950475326685
1116	7	0.22725263225748193
1116	8	0.2121848553178438
1116	9	0.32877876677478923
1116	10	0.37835582664274625
1116	11	0.22190307705940882
1116	12	0.2264825717932908
1116	13	-0.21478737456600783
1116	14	0.7250012107230148
1116	15	0.45119759818156524
1116	16	0.43113220990787937
1116	17	0.5885347642648782
1116	18	0.5389759009153678
1116	19	0.6954382785456446
1116	20	0.5804738662427056
1116	21	0.09407755195558565
1116	22	0.12554580256907144
1116	23	-0.7775890411058413
1116	24	-0.2718890885569678
1116	25	-0.9534364683314394
1201	1	0.47097175612070075
1201	2	0.6929809943320393
1201	3	0.6041608154138594
1201	4	0.5227493404045873
1201	5	0.5416691845893382
1201	6	0.39180896192053183
1201	7	0.4282237405771312
1201	8	0.33043106268087286
1201	9	0.6594773849987732
1201	10	0.9769312928713632
1201	11	0.6217583770883977
1201	12	0.7126169809149445
1201	13	0.5419654517940273
1201	14	1.1703896540508723
1201	15	0.8371037243773358
1201	16	0.6939387612654997
1201	17	0.6982376341073788
1201	18	0.6621124358462822
1201	19	0.9436644356622708
1201	20	1.0354859447043907
1201	21	0.9539893791852663
1201	22	1.0666447010133595
1201	23	0.7536101085704558
1201	24	0.9678217530702398
1201	25	1.2102102608211354
1202	1	0.35103619855399515
1202	2	0.7242316200353294
1202	3	0.5750348778600483
1202	4	0.5251536428405288
1202	5	0.45207162693453284
1202	6	0.3876445311002925
1202	7	0.2791619951752935
1202	8	0.30954538718161473
1202	9	0.8257482734774229
1202	10	0.06956616446704089
1202	11	0.4310949213641635
1202	12	0.42385010694860625
1202	13	0.3983335094908824
1202	14	1.0487766610467375
1202	15	0.7611251744872618
1202	16	0.6103873494786498
1202	17	-1.3319572048916584
1202	18	0.23743870257007416
1202	19	0.5275786051888218
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2210	18	0.6955645868186622
2210	19	0.8414983859209784
2210	20	0.9415508550314939
2210	21	1.0589696121630467
2210	22	1.1828227566755984
2210	23	1.4881125122635197
2210	24	1.2398215031479225
2210	25	0.9771493010036281
2211	1	0.14001460190239357
2211	2	-0.4708191834367277
2211	3	-0.08550247650506648
2211	4	0.2944462914363086
2211	5	0.2480271956842941
2211	6	0.23220082315173585
2211	7	0.27238037201490783
2211	8	0.3417624571910949
2211	9	0.7965756997040714
2211	10	0.8334875395333299
2211	11	0.4898566313679069
2211	12	0.3218218750138817
2211	13	0.22450639513802884
2211	14	-0.6832981707377357
2211	15	0.07435870755557517
2211	16	0.2643966912729496
2211	17	0.4111134922510189
2211	18	0.29893123255443754
2211	19	0.5694679524430981
2211	20	0.6694616106550484
2211	21	0.738420821256426
2211	22	0.013013647226614466
2211	23	0.6497927581827554
2211	24	0.06605795357469901
2211	25	0.4554559208851501
2212	1	0.11030766170049588
2212	2	-0.9459896959345393
2212	3	-0.25464520996426643
2212	4	0.2380051260318723
2212	5	0.24476994932514629
2212	6	0.22286652525021822
2212	7	0.1342718603446258
2212	8	0.12810679002789271
2212	9	-2.0679274470214537
2212	10	0.11853750789163797
2212	11	0.161584576998969
2212	12	0.07871960352707674
2212	13	0.22115222618154462
2212	14	0.7765360639554331
2212	15	0.4266724426739472
2212	16	0.40796264384155023
2212	17	0.44769260566105373
2212	18	0.22885567888116753
2212	19	-0.48330464341696455
2212	20	0.439884331975243
2212	21	0.5038604456525277
2212	22	-1.5429439836718757
2212	23	-0.24858889233920678
2212	24	0.35442326558199966
2212	25	-0.12404789918749515
2213	1	0.26154150137915266
2213	2	0.4579773018681266
2213	3	0.1662122868222653
2213	4	0.40002068649999956
2213	5	0.3658739105585127
2213	6	0.30324824396708294
2213	7	0.15060987999071784
2213	8	0.2691821252258322
2213	9	0.6577388499069059
2213	10	0.6860673043949319
2213	11	0.11248428279979095
2213	12	0.02338464800855178
2213	13	0.12485982424824726
2213	14	-1.5624714477649775
2213	15	-0.30617603938190285
2213	16	-0.0959085976612215
2213	17	-2.0686754852938236
2213	18	-0.7113518261032681
2213	19	-0.34983226035178916
2213	20	0.2764076760084109
2213	21	-0.14105420919732126
2213	22	0.42550098916401424
2213	23	0.8630397382855577
2213	24	0.48566370984534046
2213	25	-0.9625389708771266
2214	1	0.23218069512342154
2214	2	0.2614493067782462
2214	3	0.19574852321165537
2214	4	0.46934967207954514
2214	5	0.41884446564474587
2214	6	0.35581337884625697
2214	7	0.2500015985065076
2214	8	0.22872125727751325
2214	9	0.6653198642022957
2214	10	0.6786188418926571
2214	11	0.02058417998797779
2214	12	0.005030107075477019
2214	13	0.0953442711609469
2214	14	0.9553219641154342
2214	15	0.6129750629281574
2214	16	0.5380240979117659
2214	17	0.36971926507795416
2214	18	0.3583706919382763
2214	19	-0.8068870598330165
2214	20	0.25332559188468196
2214	21	0.5081912075959565
2214	22	0.6978090986263723
2214	23	1.0983030229780866
2214	24	0.8612785914330914
2214	25	1.0334154851788284
2215	1	0.006858195812245782
2215	2	0.2732203366861796
2215	3	0.17661243770398016
2215	4	0.4643585358068876
2215	5	0.3094599358381842
2215	6	0.21539946097480112
2215	7	0.18849096983169744
2215	8	0.18383762943969373
2215	9	0.35938038708422937
2215	10	0.6333082995157255
2215	11	0.37695031171180204
2215	12	0.27324867983342216
2215	13	0.1787138392037022
2215	14	0.9281938637603631
2215	15	0.37756652567710347
2215	16	0.41152763560469907
2215	17	0.5181753129472852
2215	18	0.3518898256323979
2215	19	-0.34226638057518244
2215	20	-1.556786231443816
2215	21	-1.8378624322507875
2215	22	-0.2006353562154075
2215	23	0.5966568130663106
2215	24	0.6155567305182288
2215	25	0.010738899455404294
2216	1	0.12866379717694443
2216	2	-0.19241195361729524
2216	3	0.01177137474271106
2216	4	0.29615261100008183
2216	5	0.2873809218637362
2216	6	0.09999624003050656
2216	7	0.19143930539342335
2216	8	0.11303234607910895
2216	9	0.4173189638207475
2216	10	-1.5988708798445117
2216	11	-0.4295103261169757
2216	12	-0.19455956250226336
2216	13	-0.04555881208764845
2216	14	-0.4830853048421133
2216	15	-0.4771893618321883
2216	16	0.007241129264123458
2216	17	0.3344249922832816
2216	18	0.17294633224487654
2216	19	-1.2684713620091799
2216	20	-0.9829096903355818
2216	21	-0.8894569805489922
2216	22	-0.6811532418781496
2216	23	-4.515411407682926
2216	24	-2.003060270762063
2216	25	-1.1023003332680403
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8.0
3	23.0
4	32.0
5	59.0
6	103.0
7	383.0
8	186.0
9	455.0
10	1362.0
11	3089.0
12	5871.0
13	9780.0
14	14203.0
15	18149.0
16	21543.0
17	23817.0
18	24931.0
19	25882.0
20	26710.0
21	27566.0
22	28627.0
23	30321.0
24	32710.0
25	35716.0
26	40141.0
27	46202.0
28	54244.0
29	66717.0
30	81329.0
31	100800.0
32	134124.0
33	178374.0
34	242032.0
35	334196.0
36	478178.0
37	721729.0
38	1250094.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.18746225032209	20.972476078106148	13.65878876599169	23.181272905580073
2	15.942513879519806	21.298995924469395	39.939928915966014	22.81856128004478
3	18.128003658599088	26.348664887304896	30.306644220951	25.216687233145013
4	12.72517680119879	17.611565443734722	38.01740178690019	31.6458559681663
5	13.337041510471822	37.74520978515164	35.301928506771944	13.615820197604602
6	30.661748093880586	38.18457262199792	17.812087557691108	13.341591726430382
7	25.850414554227623	32.91780588696323	23.726186014871274	17.50559354393787
8	23.42111105936264	36.90200638674687	21.717231082299534	17.959651471590952
9	24.460437859577695	17.373946509895493	22.039329640106267	36.12628599042054
10	15.040640891421345	29.750379256674165	33.73806268520871	21.470917166695784
11	31.779078253405167	25.72780944374608	23.87238135692192	18.620730945926834
12	21.14555950025746	27.338385617742784	31.272564666694592	20.243490215305172
13	26.26276961847789	24.191492596263846	27.19115331328188	22.354584471976384
14	21.29740796378877	23.322066610678053	27.969663634607272	27.4108617909259
15	22.95586728495608	30.60325602415725	24.302416069873473	22.1384606210132
16	22.182200398707046	30.81755148734089	25.87190371897436	21.1283443949777
17	20.262619279164433	30.2536975955804	28.41072070498301	21.072962420272155
18	20.400561593435132	29.78268561510645	29.380906808802965	20.435845982655458
19	21.20966792202551	28.80792319825291	29.254919970257703	20.727488909463876
20	22.26199703273891	28.58565810667851	28.592236143132162	20.560108717450422
21	22.75559830141067	28.886784443870155	27.598233415855063	20.759383838864114
22	21.660815612840505	28.382337397657885	28.92276633587209	21.034080653629523
23	22.05939997876232	28.540392128456944	28.29416311083287	21.10604478194787
24	21.610932940907198	27.939765248950877	28.498051050382955	21.951250759758974
25	22.25870822454115	28.322012698970095	28.061330679247927	21.35794839724083
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3456.0
1	3456.0
2	3865.5
3	4275.0
4	4275.0
5	4275.0
6	6017.5
7	7760.0
8	7760.0
9	7760.0
10	11849.5
11	15939.0
12	15939.0
13	15939.0
14	26124.5
15	36310.0
16	36310.0
17	36310.0
18	56906.5
19	77503.0
20	77503.0
21	77503.0
22	111185.0
23	144867.0
24	144867.0
25	144867.0
26	189040.5
27	233214.0
28	233214.0
29	233214.0
30	282321.5
31	331429.0
32	331429.0
33	331429.0
34	374006.5
35	416584.0
36	416584.0
37	416584.0
38	449596.5
39	482609.0
40	482609.0
41	482609.0
42	498709.0
43	514809.0
44	514809.0
45	514809.0
46	490483.0
47	466157.0
48	466157.0
49	466157.0
50	441968.0
51	417779.0
52	417779.0
53	417779.0
54	379011.5
55	340244.0
56	340244.0
57	340244.0
58	293641.5
59	247039.0
60	247039.0
61	247039.0
62	204295.5
63	161552.0
64	161552.0
65	161552.0
66	125228.5
67	88905.0
68	88905.0
69	88905.0
70	65411.0
71	41917.0
72	41917.0
73	41917.0
74	29711.5
75	17506.0
76	17506.0
77	17506.0
78	12070.5
79	6635.0
80	6635.0
81	6635.0
82	4459.5
83	2284.0
84	2284.0
85	2284.0
86	1495.5
87	707.0
88	707.0
89	707.0
90	433.5
91	160.0
92	160.0
93	160.0
94	97.5
95	35.0
96	35.0
97	35.0
98	23.0
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08276502172828144
2	0.02039566606875507
3	0.03207144591970906
4	0.039338017767876626
5	0.048944672075623585
6	0.019336470850208613
7	0.02436149002656856
8	0.039288752873990744
9	0.04007699117616486
10	0.026824734720862647
11	0.024632446942940905
12	0.019607427766580962
13	0.027760767704694402
14	0.04231854384797248
15	0.039608974684248975
16	0.02628282088811795
17	0.025494582585943837
18	0.030396439527589077
19	0.020198606493211542
20	0.017833891586689216
21	0.013621743159446321
22	0.02150412618118741
23	0.021257801711758003
24	0.020518828303469776
25	0.1121761633781529
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4059686.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.50726803834299
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.04774067801678	64.97229819468842
2	9.870460359748726	14.905829920907731
3	2.0726278036057355	4.694953893317411
4	0.7404038980742618	2.236235023541091
5	0.3399725561875504	1.2835199462866993
6	0.21306283750440733	0.9652675668273119
7	0.13786586268769863	0.7286912253108214
8	0.09692228965626118	0.5854669843172184
9	0.07828286314753674	0.5319832617440982
>10	0.3701926413702984	4.808271670746725
>50	0.02144449062646358	1.1040305207069885
>100	0.009451742663197317	1.2336777327470108
>500	9.169864628813286E-4	0.5159552365118399
>1k	5.239921985731323E-4	0.8334413464010721
>5k	1.3099804964327322E-4	0.6003774759456106
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATAAGCAGTGGTATCAACGCA	7701	0.18969447390758792	No Hit
GTGGTATCAACGCAGAGTACATGGG	5864	0.14444466887340549	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5464	0.13459169009622912	No Hit
GCTTATGTACTCTGCGTTGATACCA	5250	0.12932034645043974	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4479	0.11032872985743232	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	2.4632446942940906E-5
3	0.0	0.0	0.0	0.0	2.4632446942940906E-5
4	0.0	0.0	0.0	0.0	2.4632446942940906E-5
5	0.0	2.4632446942940906E-5	0.0	0.0	2.4632446942940906E-5
6	0.0	2.4632446942940906E-5	0.0	0.0	2.4632446942940906E-5
7	0.0	2.4632446942940906E-5	0.0	2.4632446942940906E-5	2.4632446942940906E-5
8	2.4632446942940906E-5	2.4632446942940906E-5	0.0	7.389734082882273E-5	2.4632446942940906E-5
9	2.4632446942940906E-5	2.4632446942940906E-5	0.0	1.2316223471470452E-4	2.4632446942940906E-5
10	2.4632446942940906E-5	2.4632446942940906E-5	0.0	1.2316223471470452E-4	2.4632446942940906E-5
11	2.4632446942940906E-5	2.4632446942940906E-5	0.0	1.2316223471470452E-4	2.4632446942940906E-5
12	2.4632446942940906E-5	2.4632446942940906E-5	0.0	1.2316223471470452E-4	2.4632446942940906E-5
13	4.926489388588181E-5	2.4632446942940906E-5	0.0	1.2316223471470452E-4	2.4632446942940906E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGCG	55	0.0030727459	12.089419	10
TCTGTCG	205	3.6379788E-12	10.194117	8
GTGCTAG	355	0.0	10.17767	1
GTGTTAG	605	0.0	9.586665	1
TGGACCG	100	2.7518396E-4	9.501874	5
TCTAGAC	300	0.0	9.185031	3
GTCGCCC	230	4.1836756E-11	9.085389	11
GTCTAAG	285	0.0	9.00766	1
ACATAAG	2245	0.0	8.803409	3
TGTCGCC	270	1.8189894E-12	8.795212	10
TTAGACA	465	0.0	8.786679	4
GTCCTAT	295	0.0	8.702315	1
TACACTG	735	0.0	8.6615715	5
CATAAGC	2235	0.0	8.587824	4
GTCTTAG	410	0.0	8.580467	1
TAATACG	100	0.002901306	8.551687	4
ACTGACG	100	0.002909085	8.549157	8
TAGACTG	445	0.0	8.54101	5
GTCTTGC	760	0.0	8.507235	1
GTATTAG	660	0.0	8.499653	1
>>END_MODULE