FastQCFastQC Report
Wed 25 May 2016
SRR1295294_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295294_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4059686
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT140840.3469233827443797No Hit
GGTATCAACGCAGAGTACTTTTTTT116890.28792867231603625No Hit
TATCAACGCAGAGTACTTTTTTTTT84850.2090063123108536No Hit
GTACATAAGCAGTGGTATCAACGCA82630.2035379090895207No Hit
GTGGTATCAACGCAGAGTACATGGG63750.1570318492612483No Hit
GCTTATGTACTCTGCGTTGATACCA58810.14486342047143547No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55770.13737515660078145No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAT500.001475553113.3293551
AGCGTCA3300.012.100863
CAGCGTC3550.011.2486872
TCGAGCG600.00589692111.0786569
GCAGCGT3700.09.7782931
CGTAGAC900.0011059759.5078183
TGAACCG1002.7349472E-49.5077025
GTACCGT1106.872728E-59.4961086
CGCCAAA1151.1165834E-49.08312117
GTCCTAA3500.08.9769131
GTACGTA850.00730573768.9609111
CCGCTTG850.00746486578.93740314
GCGTTCA850.00746486578.9374039
TATACTG9350.08.8467385
TACACGC1107.0911896E-48.6433645
TGTACCG1554.1653875E-68.5876015
GGACAAT3650.08.5855226
GCTTATG14650.08.578621
GGTATCA63950.08.5606821
CTACACG1451.6731909E-58.524254
Produced by FastQC (version 0.11.5)