FastQCFastQC Report
Wed 25 May 2016
SRR1295293_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295293_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2867248
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTACATGGG40380.14083190571586415No Hit
GTATCAACGCAGAGTACTTTTTTTT39720.1385300469300179No Hit
GGTATCAACGCAGAGTACTTTTTTT34490.12028955988460015No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGT550.003072488612.08935456
TGGACCG951.3583982E-511.0020415
GTGGTAT13200.010.8757051
TGGTATC14000.09.8404252
GGTATCA27000.09.57766151
TGACCCG800.00451050569.5017625
GCTACAC1856.684786E-99.2447973
GTCTAAG1651.0540862E-79.219141
TCTAGAC2501.8189894E-129.1215333
TAACCCG1054.4860668E-49.0492985
CTGTCGC2750.08.9808219
TAGACAG4350.08.9556855
GAATCGT850.00744580348.9400266
TAAGGGT2800.08.8230654
GGGTAAG2002.507295E-88.5565151
CTACACT3500.08.4158474
TACACTG6100.08.4113975
TCTATAC3500.08.1442263
GTCCTGG4850.08.0370461
TAGAACC2408.1490725E-97.9181364
Produced by FastQC (version 0.11.5)