FastQCFastQC Report
Wed 25 May 2016
SRR1295293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295293_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2867248
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT103230.3600316400953109No Hit
GGTATCAACGCAGAGTACTTTTTTT86940.3032175800628338No Hit
TATCAACGCAGAGTACTTTTTTTTT60740.21184076159439297No Hit
GTGGTATCAACGCAGAGTACATGGG46550.16235079769869923No Hit
ACGCAGAGTACTTTTTTTTTTTTTT41920.14620290954950532No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA400.005256207714.2599252
GACTCGT500.001504253113.2951057
ACCGTCC853.9628703E-612.2895938
AGCGTCA2500.011.7882033
GGTATCA31450.011.7144851
CTAGGAC2900.010.1622453
CAGCGTC2950.09.9900042
GTGGTAT13350.09.7694981
TGGTATC13300.09.721052
TACACCG900.00110722279.506455
ACCGCTC800.004531159.4965048
CCTACAC3450.09.3688393
CTACACT3950.09.1456054
TTAGACT2501.8189894E-129.1263514
GGCCTAC2053.9908628E-98.8233331
GTGTAGG3200.08.627421
GTACGGA1000.00285139148.5679211
TCTGTCG1451.6978282E-58.5141078
GTCTAAG2355.9299055E-108.5071541
TCCTGTA4400.08.4263192
Produced by FastQC (version 0.11.5)