FastQCFastQC Report
Wed 25 May 2016
SRR1295292_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295292_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3280233
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1313464.004166777177109No Hit
GCTTATGTACTCTGCGTTGATACCA778232.372483905868882No Hit
CTTATGTACTCTGCGTTGATACCAC498511.519739603863506No Hit
GAGTACATAAGCAGTGGTATCAACG329091.0032519031422464No Hit
TATGTACTCTGCGTTGATACCACTG320010.9755709426738893No Hit
CCCATGTACTCTGCGTTGATACCAC283480.8642069023755324No Hit
GCGTTGATACCACTGCTTATGTACT257060.7836638433916127No Hit
CATAAGCAGTGGTATCAACGCAGAG208980.637088889722163No Hit
ACTCTGCGTTGATACCACTGCTTAT187520.5716667078222797No Hit
CATGTACTCTGCGTTGATACCACTG183850.5604784782056641No Hit
CTGCTTATGTACTCTGCGTTGATAC181670.5538326088421158No Hit
ACGCAGAGTACATAAGCAGTGGTAT173700.5295355543340976No Hit
GTATCAACGCAGAGTACATAAGCAG167340.5101466877505347No Hit
GTATCAACGCAGAGTACTTTTTTTT142450.43426793157681176No Hit
TATCAACGCAGAGTACATAAGCAGT137350.4187202555428227No Hit
GGTATCAACGCAGAGTACATAAGCA131160.3998496448270596No Hit
GTACATGATAAGCAGTGGTATCAAC128200.3908258955994894No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA123860.3775951281509576No Hit
ATCATGTACTCTGCGTTGATACCAC119070.3629925069347208No Hit
GTGGTATCAACGCAGAGTACATGGG115610.3524444757430341No Hit
AAGCAGTGGTATCAACGCAGAGTAC107340.32723285205654595No Hit
GTACATGGGAAGCAGTGGTATCAAC106190.3237270035390779No Hit
GTGGTATCAACGCAGAGTACATAAG101210.3085451551764768No Hit
GTACATGGGATAAGCAGTGGTATCA94640.288516090167985No Hit
GGTATCAACGCAGAGTACTTTTTTT91390.2786082574012273No Hit
GCAGTGGTATCAACGCAGAGTACAT82300.250896811293588No Hit
TATCAACGCAGAGTACTTTTTTTTT82130.25037855542578835No Hit
GCTTATCCCATGTACTCTGCGTTGA80630.24580570953343864No Hit
GTACTGGTTCACTATCGGTCAGTCA80490.24537891058348602No Hit
GTACTCTGCGTTGATACCACTGCTT77100.23504427886677565No Hit
ACATAAGCAGTGGTATCAACGCAGA75790.2310506601207902No Hit
CCACTGCTTATGTACTCTGCGTTGA68950.21019848285167547No Hit
GATACCACTGCTTATGTACTCTGCG66990.20422329755233848No Hit
GTTGATACCACTGCTTATGTACTCT64730.19733354307453158No Hit
ATACCACTGCTTATGTACTCTGCGT60320.1838893761510234No Hit
GGATACCACGTGTCCCGCCCTACTC60260.18370646231532944No Hit
GGATAAGCAGTGGTATCAACGCAGA54830.16715276018502345No Hit
ATACAGGGTGACAGCCCCGTACACA54000.16462245212458992No Hit
CAGTGGTATCAACGCAGAGTACATA50620.15431830604716187No Hit
GTTAATGATAGTGTGTCGAAACACA50480.15389150709720925No Hit
TGATACCACTGCTTATGTACTCTGC48310.14727612337294332No Hit
GGGATAAGCAGTGGTATCAACGCAG48250.14709320953724933No Hit
TACCACTGCTTATGTACTCTGCGTT47110.14361784665906355No Hit
GAGTACATGATAAGCAGTGGTATCA46690.14233744980920562No Hit
GTACTTTTTTTTTTTTTTTTTTTTT46270.1410570529593477No Hit
GAGTACATGGGATAAGCAGTGGTAT44630.13605740811704534No Hit
ATCATTAACTGAATCCATAGGTTAA44600.13596595119919835No Hit
GAGTACATGGGAAGCAGTGGTATCA44010.1341672984815408No Hit
GATAAGCAGTGGTATCAACGCAGAG43010.13111873455330764No Hit
GCAGAGTACATAAGCAGTGGTATCA42300.1289542541642621No Hit
ACCCTGTATCGCGCGCCTTTCCAGA40000.12194255712932586No Hit
CCCCATGTACTCTGCGTTGATACCA39890.12160721509722024No Hit
GTATCAACGCAGAGTACATGGGATA38440.11718679740128216No Hit
GCTTATCATGTACTCTGCGTTGATA38230.11654659897635321No Hit
GGTATCAACGCAGAGTACATGGGGG37850.1153881446836246No Hit
GGTATCAACGCAGAGTACATGGGAA37480.11426017603017834No Hit
ACGCAGAGTACTTTTTTTTTTTTTT37450.11416871911233135No Hit
GTATCAACGCAGAGTACATGGGAAG37250.11355900632668472No Hit
GCGTTGATACCACTGCTTATCCCAT36220.11041898548060458No Hit
GTATTTAGCCTTGGAGGATGGTCCC35800.10913858863074664No Hit
GGTATCAACGCAGAGTACATGGGAT35750.108986160434335No Hit
GCGTTGATACCACTGCTTCCCATGT35630.10862033276294702No Hit
GGTTAATGAGGCGAACCGGGGGAAC35120.10706556515954811No Hit
GTACACAAGCAGTGGTATCAACGCA35110.1070350795202658No Hit
GCGTTGATACCACTGCTTATCATGT35100.10700459388098345No Hit
CCCATATTCAGACAGGATACCACGT33180.10115135113877582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTGTA4150.014.87722511
GTCTACG456.728566E-414.7883541
GTTGTAT4450.014.301167512
CCCCTAT759.604337E-713.9433051
ATTCGTA759.70731E-713.93012418
TTGTATC4500.013.72010713
GCAGTTG4650.013.6858549
TAGACTT1051.369699E-913.5732695
CTTCGTT3150.013.56933812
TGTATCA4750.013.39834314
CAGTTGT4700.013.33816610
GACGCTA500.001501909513.29795213
TAGTACT1403.6379788E-1212.8946074
TTCGTAT751.4647803E-512.67921519
ATGTACG1501.8189894E-1212.6683854
CGACTAG751.48281215E-512.66413517
TTCGTTG3250.012.56729413
TACTTGC853.9559145E-612.291667
GCAACCG1551.8189894E-1212.2559899
TACGCTG1551.8189894E-1212.2556157
Produced by FastQC (version 0.11.5)