##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295292_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3280233 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.010196836627156 31.0 30.0 33.0 26.0 34.0 2 29.760942591578097 31.0 30.0 34.0 25.0 34.0 3 30.338483272377296 31.0 30.0 34.0 26.0 34.0 4 33.455833777661525 35.0 35.0 37.0 27.0 37.0 5 34.15834728813471 35.0 35.0 37.0 30.0 37.0 6 34.22085992062149 37.0 35.0 37.0 30.0 37.0 7 34.17885985538222 37.0 35.0 37.0 30.0 37.0 8 34.135241002697065 37.0 35.0 37.0 29.0 37.0 9 35.08078298096507 39.0 35.0 39.0 27.0 39.0 10 35.00492800358999 39.0 35.0 39.0 27.0 39.0 11 35.468154243921084 39.0 35.0 39.0 28.0 39.0 12 35.481963019090415 39.0 35.0 39.0 28.0 39.0 13 35.2575143290126 39.0 35.0 39.0 27.0 39.0 14 35.502032020286364 39.0 34.0 41.0 25.0 41.0 15 36.05739348393849 39.0 36.0 41.0 27.0 41.0 16 36.07424045791869 39.0 36.0 41.0 27.0 41.0 17 36.089861299486955 39.0 36.0 41.0 27.0 41.0 18 36.04216773625532 39.0 36.0 41.0 27.0 41.0 19 36.12518226601586 39.0 35.0 41.0 27.0 41.0 20 36.162947875958814 39.0 35.0 41.0 27.0 41.0 21 36.16839078199628 39.0 35.0 41.0 27.0 41.0 22 35.97426127961032 39.0 35.0 41.0 27.0 41.0 23 35.63746416794173 39.0 34.0 41.0 25.0 41.0 24 35.966946555320916 39.0 34.0 41.0 27.0 41.0 25 34.932040803199044 38.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38897833553885874 1101 2 0.6812288768280901 1101 3 0.5223607634390746 1101 4 0.4921427121920061 1101 5 0.3840261301291221 1101 6 0.3357247717722487 1101 7 0.33362323703312313 1101 8 0.4152942329921814 1101 9 0.485209474134102 1101 10 0.6438531075627125 1101 11 0.6348845421138734 1101 12 0.5645522125178033 1101 13 0.5207693067377406 1101 14 1.21723772622925 1101 15 0.9161457611264083 1101 16 0.8262160895095221 1101 17 0.9932878737058246 1101 18 0.9395395166320384 1101 19 0.9290861481136972 1101 20 1.0285017398741942 1101 21 1.0184035880953672 1101 22 0.7762761911378107 1101 23 -1.5899639217958352 1101 24 0.1701877288282816 1101 25 0.8561206753329529 1102 1 0.42108886874857276 1102 2 0.48356803241171775 1102 3 0.44185832391379876 1102 4 0.5043573889004449 1102 5 0.4125510708033957 1102 6 0.31052483754868376 1102 7 0.267774241696209 1102 8 0.35403187480841325 1102 9 0.7075120434693289 1102 10 1.058726235955966 1102 11 0.7380877143871345 1102 12 0.6837705830884531 1102 13 0.6658152536247854 1102 14 1.2764769664204678 1102 15 0.9597906623348251 1102 16 0.8444026490646621 1102 17 1.0561374294307058 1102 18 1.0534855420643368 1102 19 1.146434645391139 1102 20 1.0497075363847799 1102 21 0.949832315954076 1102 22 0.893255283248692 1102 23 1.192732859061401 1102 24 1.0979446651922302 1102 25 0.9012089828514362 1103 1 -0.20528677959772423 1103 2 -1.3727368462829936 1103 3 -0.2754059689549244 1103 4 0.11474266925561949 1103 5 -0.014043795867117126 1103 6 0.1157001845389587 1103 7 0.13794114170561045 1103 8 0.36868444182889704 1103 9 0.9228877226469052 1103 10 0.9541046267053162 1103 11 0.6782319415457039 1103 12 0.479431119600207 1103 13 0.2712193054877474 1103 14 -0.6617446535816001 1103 15 0.12784308974494252 1103 16 0.23607258990812596 1103 17 0.675343816407576 1103 18 0.8362963510376602 1103 19 0.819115322635831 1103 20 0.7930859255530436 1103 21 0.8761455507168989 1103 22 0.42875533107670805 1103 23 1.2352074963787985 1103 24 1.0591946839676893 1103 25 1.007098232141331 1104 1 0.23862581743055244 1104 2 0.2529655444124579 1104 3 -0.04559344258509057 1104 4 0.007087004634151128 1104 5 0.22014655493427426 1104 6 0.16606959095718565 1104 7 0.21731949988801347 1104 8 0.18981640028783175 1104 9 -1.8542615903395046 1104 10 -0.29390509264340636 1104 11 -0.008372589744801928 1104 12 0.27022778086158183 1104 13 0.3816897178992278 1104 14 -1.3544302845005163 1104 15 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0.18159673963356937 2213 5 0.11079236960001282 2213 6 0.004748208724606684 2213 7 0.0633735718001418 2213 8 0.008480055169549416 2213 9 0.3957508659267077 2213 10 0.3922668768200168 2213 11 -0.1360793402343674 2213 12 -0.12798712190367212 2213 13 -0.08069207002154855 2213 14 -2.1815950481531914 2213 15 -0.7837040557291743 2213 16 -0.34872362018987957 2213 17 -2.325983558111872 2213 18 -0.9853087839225765 2213 19 -0.7312501775618117 2213 20 -0.00790151626311797 2213 21 -0.3511294909192486 2213 22 0.21899019068543168 2213 23 0.6096023898877192 2213 24 0.384441955034589 2213 25 -1.1578225086261753 2214 1 0.060620842454607526 2214 2 -0.005231977795926213 2214 3 -0.12018414452660053 2214 4 0.2228794205928608 2214 5 0.09239961488012227 2214 6 0.052228081607658794 2214 7 0.038699477660983916 2214 8 -0.08356505431457606 2214 9 0.2911760136166066 2214 10 0.29782406578757303 2214 11 -0.2938050844677491 2214 12 -0.2115453841032462 2214 13 -0.19532525923974475 2214 14 0.572052213578857 2214 15 0.2449844161844439 2214 16 0.13098980436817698 2214 17 0.05250806087790494 2214 18 0.08195298025879083 2214 19 -0.989940063314009 2214 20 -0.07934695735665542 2214 21 0.25617242306474 2214 22 0.4313150072479104 2214 23 0.9054706072782537 2214 24 0.6346817452723599 2214 25 0.5168415842116048 2215 1 -0.23401731998339415 2215 2 0.19021136645078585 2215 3 0.031508350267646534 2215 4 0.28348304894713294 2215 5 0.12078592063807037 2215 6 0.1656003577701881 2215 7 0.1159479136221293 2215 8 0.14393684614600488 2215 9 0.21694166077877952 2215 10 0.5865828331833143 2215 11 0.19620942974236044 2215 12 0.08729353415745322 2215 13 -0.11217977834887449 2215 14 0.8069438272995342 2215 15 0.2679318581788195 2215 16 0.29574905329469914 2215 17 0.33210295713622173 2215 18 0.2864850528302796 2215 19 -0.4978610905033207 2215 20 -1.7472737690831863 2215 21 -1.9385860174363572 2215 22 -0.35213755965700244 2215 23 0.3381059706258327 2215 24 0.494791105186664 2215 25 -0.1843091443955771 2216 1 -0.07852616860914807 2216 2 -0.3317784490736777 2216 3 -0.27343632564109654 2216 4 0.18397260754631617 2216 5 0.08701695058793035 2216 6 -0.09956578455259546 2216 7 0.04697845812899004 2216 8 -0.15231300447427998 2216 9 0.0636423160360593 2216 10 -1.8856544219167617 2216 11 -0.7424604103380119 2216 12 -0.37097315100248096 2216 13 -0.29894054436857687 2216 14 -0.8113815205980615 2216 15 -0.6492496284074178 2216 16 -0.33497229411111107 2216 17 -0.014552108292747334 2216 18 -0.15150404348073465 2216 19 -1.6048995392640322 2216 20 -1.2124545637890094 2216 21 -1.1024531782745086 2216 22 -1.062600159227614 2216 23 -4.864469458240656 2216 24 -2.4435781497842584 2216 25 -1.6868709641245232 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 11.0 5 28.0 6 55.0 7 277.0 8 162.0 9 370.0 10 934.0 11 2179.0 12 4613.0 13 8544.0 14 13424.0 15 19646.0 16 25695.0 17 29346.0 18 29207.0 19 28027.0 20 27004.0 21 25836.0 22 24195.0 23 24101.0 24 24206.0 25 25357.0 26 27426.0 27 31193.0 28 36239.0 29 45154.0 30 56669.0 31 71562.0 32 97824.0 33 134862.0 34 188832.0 35 269667.0 36 398787.0 37 619675.0 38 989119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.19699410537107 18.360457169357694 11.877799338532324 20.564749386738914 2 13.818774015754274 23.25377528724382 38.694689442489945 24.23276125451196 3 20.18801762710996 30.654899402823865 30.078795047795744 19.078287922270434 4 12.533233386661266 20.010600527830654 32.96867842890175 34.48748765660633 5 10.242172614971985 44.689590051412466 32.418307191980425 12.649930141635126 6 28.418777098184613 33.79627653411551 23.257605785824488 14.527340581875393 7 23.803376141373253 38.02129038828492 19.488889200579827 18.686444269762 8 22.329333191015692 36.52789679937787 23.752245314954028 17.390524694652417 9 27.722795829043477 21.11924895457717 17.319100375229944 33.8388548411494 10 15.223876227964334 28.630074038431925 28.45440771236461 27.691642021239126 11 28.674438622880018 29.161972198176038 20.159417289284047 22.00417188965989 12 25.301144940761944 28.474360565326506 24.366312745580203 21.858181748331344 13 28.545335791427124 22.028226202360965 29.853917022868686 19.57252098334322 14 27.856266058753114 25.36673808126015 23.555958798100015 23.22103706188672 15 30.91541862821847 24.3172244401553 23.558383314339178 21.20897361728705 16 23.710149753257177 26.60123651940157 28.342066297345227 21.346547429996026 17 19.6031659153965 32.555006667059 26.371092193244394 21.47073522430011 18 20.54474254921046 24.737206743705524 35.19051138872422 19.52753931835979 19 22.757590106647047 32.72427300448791 23.200557856411734 21.317579032453303 20 22.041569365666078 29.449647551612596 28.532998187983242 19.975784894738077 21 23.229907517704202 31.316902402564 24.033170816779077 21.42001926295272 22 21.030043965365575 29.290373569921215 22.30826859273936 27.371313871973857 23 25.127043164489265 25.161376682601567 23.82898614889526 25.88259400401391 24 20.084875376261728 29.663450045614876 22.031099830712712 28.220574747410687 25 23.784442672962616 31.159120845851373 23.276111486160673 21.78032499502534 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13769.0 1 13769.0 2 11564.0 3 9359.0 4 9359.0 5 9359.0 6 13711.5 7 18064.0 8 18064.0 9 18064.0 10 18039.5 11 18015.0 12 18015.0 13 18015.0 14 20646.5 15 23278.0 16 23278.0 17 23278.0 18 31878.5 19 40479.0 20 40479.0 21 40479.0 22 54525.0 23 68571.0 24 68571.0 25 68571.0 26 89835.0 27 111099.0 28 111099.0 29 111099.0 30 128978.5 31 146858.0 32 146858.0 33 146858.0 34 164428.5 35 181999.0 36 181999.0 37 181999.0 38 228896.0 39 275793.0 40 275793.0 41 275793.0 42 539735.5 43 803678.0 44 803678.0 45 803678.0 46 656436.5 47 509195.0 48 509195.0 49 509195.0 50 434958.5 51 360722.0 52 360722.0 53 360722.0 54 301793.5 55 242865.0 56 242865.0 57 242865.0 58 209799.0 59 176733.0 60 176733.0 61 176733.0 62 156003.5 63 135274.0 64 135274.0 65 135274.0 66 104133.0 67 72992.0 68 72992.0 69 72992.0 70 58131.0 71 43270.0 72 43270.0 73 43270.0 74 30996.0 75 18722.0 76 18722.0 77 18722.0 78 12613.5 79 6505.0 80 6505.0 81 6505.0 82 4295.5 83 2086.0 84 2086.0 85 2086.0 86 1393.5 87 701.0 88 701.0 89 701.0 90 429.5 91 158.0 92 158.0 93 158.0 94 96.0 95 34.0 96 34.0 97 34.0 98 24.0 99 14.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08148811380167202 2 0.018748668158633852 3 0.0294491275467322 4 0.03508897081396352 5 0.04438709079507462 6 0.015029420166189415 7 0.019236438387151156 8 0.03606451127099813 9 0.03588159743530414 10 0.021949660283278657 11 0.020242464483468094 12 0.015242819641165733 13 0.02216305975825498 14 0.03835093421717299 15 0.035180427731810515 16 0.021492375694043685 17 0.020608292154856073 18 0.02588230775069942 19 0.016340302655329667 20 0.014084365348437138 21 0.011035801420203991 22 0.01847429740509287 23 0.01948032350140981 24 0.018321869208681212 25 0.11063238495558089 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3280233.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.719632324746033 #Duplication Level Percentage of deduplicated Percentage of total 1 74.3821945961556 13.180251397515885 2 8.381339569760984 2.970285111300194 3 3.6746824312300768 1.953420647748024 4 2.230547961415418 1.5809795903597201 5 1.5429402133677443 1.367016663997081 6 1.131768285507459 1.2032710737600218 7 0.909430881268437 1.1280346588592527 8 0.7379469507765062 1.046091891434172 9 0.5982094400413133 0.954004619765185 >10 5.383342130729233 19.62128847600742 >50 0.6293547892287501 7.699314684352639 >100 0.33428947795097325 10.816375201837584 >500 0.028692549314092507 3.517112399920607 >1k 0.02834685594886248 10.631857677141268 >5k 0.0029383936044552568 3.662012505847636 >10k+ 0.003975473700145347 18.66868340015333 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATAAGCAGTGGTATCAACGCA 131346 4.004166777177109 No Hit GCTTATGTACTCTGCGTTGATACCA 77823 2.372483905868882 No Hit CTTATGTACTCTGCGTTGATACCAC 49851 1.519739603863506 No Hit GAGTACATAAGCAGTGGTATCAACG 32909 1.0032519031422464 No Hit TATGTACTCTGCGTTGATACCACTG 32001 0.9755709426738893 No Hit CCCATGTACTCTGCGTTGATACCAC 28348 0.8642069023755324 No Hit GCGTTGATACCACTGCTTATGTACT 25706 0.7836638433916127 No Hit CATAAGCAGTGGTATCAACGCAGAG 20898 0.637088889722163 No Hit ACTCTGCGTTGATACCACTGCTTAT 18752 0.5716667078222797 No Hit CATGTACTCTGCGTTGATACCACTG 18385 0.5604784782056641 No Hit CTGCTTATGTACTCTGCGTTGATAC 18167 0.5538326088421158 No Hit ACGCAGAGTACATAAGCAGTGGTAT 17370 0.5295355543340976 No Hit GTATCAACGCAGAGTACATAAGCAG 16734 0.5101466877505347 No Hit GTATCAACGCAGAGTACTTTTTTTT 14245 0.43426793157681176 No Hit TATCAACGCAGAGTACATAAGCAGT 13735 0.4187202555428227 No Hit GGTATCAACGCAGAGTACATAAGCA 13116 0.3998496448270596 No Hit GTACATGATAAGCAGTGGTATCAAC 12820 0.3908258955994894 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 12386 0.3775951281509576 No Hit ATCATGTACTCTGCGTTGATACCAC 11907 0.3629925069347208 No Hit GTGGTATCAACGCAGAGTACATGGG 11561 0.3524444757430341 No Hit AAGCAGTGGTATCAACGCAGAGTAC 10734 0.32723285205654595 No Hit GTACATGGGAAGCAGTGGTATCAAC 10619 0.3237270035390779 No Hit GTGGTATCAACGCAGAGTACATAAG 10121 0.3085451551764768 No Hit GTACATGGGATAAGCAGTGGTATCA 9464 0.288516090167985 No Hit GGTATCAACGCAGAGTACTTTTTTT 9139 0.2786082574012273 No Hit GCAGTGGTATCAACGCAGAGTACAT 8230 0.250896811293588 No Hit TATCAACGCAGAGTACTTTTTTTTT 8213 0.25037855542578835 No Hit GCTTATCCCATGTACTCTGCGTTGA 8063 0.24580570953343864 No Hit GTACTGGTTCACTATCGGTCAGTCA 8049 0.24537891058348602 No Hit GTACTCTGCGTTGATACCACTGCTT 7710 0.23504427886677565 No Hit ACATAAGCAGTGGTATCAACGCAGA 7579 0.2310506601207902 No Hit CCACTGCTTATGTACTCTGCGTTGA 6895 0.21019848285167547 No Hit GATACCACTGCTTATGTACTCTGCG 6699 0.20422329755233848 No Hit GTTGATACCACTGCTTATGTACTCT 6473 0.19733354307453158 No Hit ATACCACTGCTTATGTACTCTGCGT 6032 0.1838893761510234 No Hit GGATACCACGTGTCCCGCCCTACTC 6026 0.18370646231532944 No Hit GGATAAGCAGTGGTATCAACGCAGA 5483 0.16715276018502345 No Hit ATACAGGGTGACAGCCCCGTACACA 5400 0.16462245212458992 No Hit CAGTGGTATCAACGCAGAGTACATA 5062 0.15431830604716187 No Hit GTTAATGATAGTGTGTCGAAACACA 5048 0.15389150709720925 No Hit TGATACCACTGCTTATGTACTCTGC 4831 0.14727612337294332 No Hit GGGATAAGCAGTGGTATCAACGCAG 4825 0.14709320953724933 No Hit TACCACTGCTTATGTACTCTGCGTT 4711 0.14361784665906355 No Hit GAGTACATGATAAGCAGTGGTATCA 4669 0.14233744980920562 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 4627 0.1410570529593477 No Hit GAGTACATGGGATAAGCAGTGGTAT 4463 0.13605740811704534 No Hit ATCATTAACTGAATCCATAGGTTAA 4460 0.13596595119919835 No Hit GAGTACATGGGAAGCAGTGGTATCA 4401 0.1341672984815408 No Hit GATAAGCAGTGGTATCAACGCAGAG 4301 0.13111873455330764 No Hit GCAGAGTACATAAGCAGTGGTATCA 4230 0.1289542541642621 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 4000 0.12194255712932586 No Hit CCCCATGTACTCTGCGTTGATACCA 3989 0.12160721509722024 No Hit GTATCAACGCAGAGTACATGGGATA 3844 0.11718679740128216 No Hit GCTTATCATGTACTCTGCGTTGATA 3823 0.11654659897635321 No Hit GGTATCAACGCAGAGTACATGGGGG 3785 0.1153881446836246 No Hit GGTATCAACGCAGAGTACATGGGAA 3748 0.11426017603017834 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3745 0.11416871911233135 No Hit GTATCAACGCAGAGTACATGGGAAG 3725 0.11355900632668472 No Hit GCGTTGATACCACTGCTTATCCCAT 3622 0.11041898548060458 No Hit GTATTTAGCCTTGGAGGATGGTCCC 3580 0.10913858863074664 No Hit GGTATCAACGCAGAGTACATGGGAT 3575 0.108986160434335 No Hit GCGTTGATACCACTGCTTCCCATGT 3563 0.10862033276294702 No Hit GGTTAATGAGGCGAACCGGGGGAAC 3512 0.10706556515954811 No Hit GTACACAAGCAGTGGTATCAACGCA 3511 0.1070350795202658 No Hit GCGTTGATACCACTGCTTATCATGT 3510 0.10700459388098345 No Hit CCCATATTCAGACAGGATACCACGT 3318 0.10115135113877582 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.048563928233147E-5 0.0 2 0.0 0.0 0.0 3.048563928233147E-5 0.0 3 0.0 0.0 0.0 3.048563928233147E-5 0.0 4 0.0 0.0 0.0 3.048563928233147E-5 0.0 5 1.2194255712932587E-4 0.0 0.0 3.048563928233147E-5 0.0 6 1.5242819641165734E-4 0.0 0.0 3.048563928233147E-5 0.0 7 1.5242819641165734E-4 0.0 0.0 3.048563928233147E-5 0.0 8 1.8291383569398882E-4 0.0 0.0 3.048563928233147E-5 0.0 9 1.8291383569398882E-4 3.048563928233147E-5 0.0 3.048563928233147E-5 0.0 10 2.4388511425865175E-4 3.048563928233147E-5 0.0 3.048563928233147E-5 0.0 11 2.4388511425865175E-4 3.048563928233147E-5 0.0 6.097127856466294E-5 0.0 12 2.4388511425865175E-4 3.048563928233147E-5 0.0 6.097127856466294E-5 0.0 13 2.4388511425865175E-4 3.048563928233147E-5 0.0 9.145691784699441E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTTGTA 415 0.0 14.877225 11 GTCTACG 45 6.728566E-4 14.788354 1 GTTGTAT 445 0.0 14.3011675 12 CCCCTAT 75 9.604337E-7 13.943305 1 ATTCGTA 75 9.70731E-7 13.930124 18 TTGTATC 450 0.0 13.720107 13 GCAGTTG 465 0.0 13.685854 9 TAGACTT 105 1.369699E-9 13.573269 5 CTTCGTT 315 0.0 13.569338 12 TGTATCA 475 0.0 13.398343 14 CAGTTGT 470 0.0 13.338166 10 GACGCTA 50 0.0015019095 13.297952 13 TAGTACT 140 3.6379788E-12 12.894607 4 TTCGTAT 75 1.4647803E-5 12.679215 19 ATGTACG 150 1.8189894E-12 12.668385 4 CGACTAG 75 1.48281215E-5 12.664135 17 TTCGTTG 325 0.0 12.567294 13 TACTTGC 85 3.9559145E-6 12.29166 7 GCAACCG 155 1.8189894E-12 12.255989 9 TACGCTG 155 1.8189894E-12 12.255615 7 >>END_MODULE