FastQCFastQC Report
Wed 25 May 2016
SRR1295292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295292_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3280233
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCA1361444.150436874453735No Hit
GCTTATGTACTCTGCGTTGATACCA864782.6363371138574605No Hit
CTTATGTACTCTGCGTTGATACCAC571671.742772540853043No Hit
GTATCAACGCAGAGTACTTTTTTTT366831.1183047057937652No Hit
GAGTACATAAGCAGTGGTATCAACG352361.0741919857522315No Hit
CCCATGTACTCTGCGTTGATACCAC342481.0440721741412882No Hit
GCGTTGATACCACTGCTTATGTACT290830.8866138472480461No Hit
TATGTACTCTGCGTTGATACCACTG289030.8811264321772264No Hit
CATAAGCAGTGGTATCAACGCAGAG255450.7787556554671573No Hit
GGTATCAACGCAGAGTACTTTTTTT249170.7596106739978532No Hit
TATCAACGCAGAGTACTTTTTTTTT231380.7053767217145855No Hit
ACTCTGCGTTGATACCACTGCTTAT221910.6765068213142177No Hit
ACGCAGAGTACATAAGCAGTGGTAT208560.6358084928723051No Hit
CATGTACTCTGCGTTGATACCACTG206990.6310222475049792No Hit
CTGCTTATGTACTCTGCGTTGATAC206890.6307173911121557No Hit
GTATCAACGCAGAGTACATAAGCAG181660.5538021232028335No Hit
GTACTTTTTTTTTTTTTTTTTTTTT162680.49594037984496836No Hit
TATCAACGCAGAGTACATAAGCAGT154520.4710640981905858No Hit
GGTATCAACGCAGAGTACATAAGCA139010.4237808716636897No Hit
ATCATGTACTCTGCGTTGATACCAC133180.40600774396209055No Hit
ACGCAGAGTACTTTTTTTTTTTTTT126600.3859481933143164No Hit
GTACATGATAAGCAGTGGTATCAAC123440.37631473130109966No Hit
AAGCAGTGGTATCAACGCAGAGTAC122970.3748819062548301No Hit
GTGGTATCAACGCAGAGTACATGGG117880.35936471586012336No Hit
GTGGTATCAACGCAGAGTACATAAG103620.31589219424351866No Hit
GTACATGGGAAGCAGTGGTATCAAC101050.3080573849479595No Hit
ACATAAGCAGTGGTATCAACGCAGA91890.2801325393653439No Hit
GTACATGGGATAAGCAGTGGTATCA90460.2757730929479704No Hit
GCAGTGGTATCAACGCAGAGTACAT89650.2733037561661016No Hit
GCTTATCCCATGTACTCTGCGTTGA87140.2656518607062364No Hit
GTACTGGTTCACTATCGGTCAGTCA81030.2470251351047319No Hit
CCACTGCTTATGTACTCTGCGTTGA79840.24339734403013444No Hit
GTACTCTGCGTTGATACCACTGCTT76770.2340382527704587No Hit
GATACCACTGCTTATGTACTCTGCG76620.23358096818122373No Hit
GAGTACTTTTTTTTTTTTTTTTTTT70330.21440550107263723No Hit
ATACCACTGCTTATGTACTCTGCGT68280.20815594501975926No Hit
GGATACCACGTGTCCCGCCCTACTC65680.2002296788063531No Hit
GTTGATACCACTGCTTATGTACTCT63830.19458983553912176No Hit
ATACAGGGTGACAGCCCCGTACACA63180.19260826898577021No Hit
GGATAAGCAGTGGTATCAACGCAGA60710.18507831608303435No Hit
CAGTGGTATCAACGCAGAGTACATA60510.18446860329738773No Hit
GGGATAAGCAGTGGTATCAACGCAG55970.17062812306320924No Hit
TACCACTGCTTATGTACTCTGCGTT54330.16562847822090687No Hit
TGATACCACTGCTTATGTACTCTGC52060.15870823810381762No Hit
GTTAATGATAGTGTGTCGAAACACA50010.15245868205093968No Hit
GAGTACATGATAAGCAGTGGTATCA49690.15148314159390508No Hit
GATAAGCAGTGGTATCAACGCAGAG49190.1499588596297885No Hit
CCCCATGTACTCTGCGTTGATACCA48950.14922720428701255No Hit
GCAGAGTACATAAGCAGTGGTATCA48850.14892234789418923No Hit
GCAGAGTACTTTTTTTTTTTTTTTT48320.14730660901222564No Hit
ATCATTAACTGAATCCATAGGTTAA48140.1467578675051437No Hit
GAGTACATGGGATAAGCAGTGGTAT47330.14428853072327485No Hit
GAGTACATGGGAAGCAGTGGTATCA46500.14175822266284133No Hit
ACCCTGTATCGCGCGCCTTTCCAGA45300.13809994594896155No Hit
GCTTATCATGTACTCTGCGTTGATA42350.12910668236067377No Hit
GCGTTGATACCACTGCTTCCCATGT40550.1236192672898541No Hit
GTATCAACGCAGAGTACATGGGAAG39860.12151575817937323No Hit
GGTATCAACGCAGAGTACATGGGGG39770.12124138742583225No Hit
CCCATATTCAGACAGGATACCACGT39700.12102798795085594No Hit
GCGTTGATACCACTGCTTATCCCAT39280.119747591100998No Hit
GGTATCAACGCAGAGTACATGGGAA39060.11907690703678671No Hit
ATCAACGCAGAGTACATAAGCAGTG38860.11846719425114009No Hit
CAGTGGTATCAACGCAGAGTACATG38800.1182842804154461No Hit
GTATCAACGCAGAGTACATGGGATA38320.11682096972989418No Hit
GCGTTGATACCACTGCTTATCATGT37830.11532717340505994No Hit
GTACACAAGCAGTGGTATCAACGCA37400.11401629091591968No Hit
GGTATCAACGCAGAGTACATGGGAT37010.11282735098390875No Hit
ACTTAGATGTTTCAGTTCCCCCGGT35480.10816304817371206No Hit
ATCAACGCAGAGTACTTTTTTTTTT34850.10624245289892517No Hit
GGTTAATGAGGCGAACCGGGGGAAC34530.10526691244189056No Hit
GTATTTAGCCTTGGAGGATGGTCCC34290.10453525709911461No Hit
ACGCAGAGTACATGGGATAAGCAGT33960.10352923100279768No Hit
CTATCGGTCAGTCAGGAGTATTTAG33760.10291951821715103No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA32920.1003587245174352No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAG307.6292094E-419.0353661
TACTAGA802.1827873E-1117.8265932
AAATCGC3550.013.37494817
CGTAACA500.001491253413.3105232
GGACCGT1800.013.1897876
CCGCTTG655.475757E-513.14880312
GTACTAG1102.6757334E-912.9786591
TTAGGTT1550.012.8811514
TTAGACA2450.012.4179794
AGGACCG1001.4295074E-712.3597715
GCTCTCG853.9647202E-612.28921618
CTAGGAC701.0835832E-412.2239493
CTCTGTG701.09568384E-412.2094157
ATTAGAC2500.012.169623
CAGACCC1353.6743586E-1011.9724284
TAGGACT802.8455112E-511.8843954
ACTTAGG657.903316E-411.7140731
AGGACTA657.974505E-411.7015585
ATTCGTA658.054488E-411.68764618
GCTACAC1552.5465852E-1111.6543753
Produced by FastQC (version 0.11.5)